Browsing by Author "Celik, Ismail"

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Abcisic Acid and Gibberellic Acid Cause Increased Lipid Peroxidation and Fluctuated Antioxidant Defense Systems of Various Tissues in Rats
(Elsevier Science Bv, 2007) Celik, Ismail; Turker, Musa; Tuluce, Yasin
The study was aimed at demonstrating changes in the antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] forming an antioxidative barrier and oxidative stress parameter (Malondialdehyde = MDA) in the various tissues of Sprague-Dawley rats which were administrated plant growth regulators (PGRs) [Abcisic acid (ABA) and Gibberellic acid (GA(3))] during 25 days. Seventy five parts per million of ABA and GA(3) as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 25 days continuously. The PGRs treatments caused different effects on antioxidant defense systems and MDA content of experimented rats compared to controls. The lipid peroxidation end product MDA significantly increased in the spleen and lungs of rats treated with ABA and GA(3) without significantly change in the other tissues. The GSH levels were significantly increased in the lungs and stomach of rats treated with ABA without any change in the tissues of rats treated with GA(3). Antioxidant enzyme activities such as SOD significantly increased in the spleen of rats treated with ABA and GA(3). Meanwhile, SOD significantly increased in the kidney of rats treated with GA. CAT significantly decreased in the lungs treated with ABA but did not change significantly in all the rest of rat tissues treated with both the PGRs. On the other hand, the ancillary enzyme GR activity decreased in the spleen and increased in the kidney with GA(3) treatment. The drug metabolizing enzyme GST activity significantly decreased in the heart of rats treated with GA(3) but increased in the spleen and lungs of rats treated with both PGRs. The observations presented led us to conclude that administration of subacute ABA and GA(3) promotes lipid peroxidatin content and alters in the antioxidative systems in the rat's various tissues. These data, along with changes, suggest that the PGRs produced oxidative stress in rats during the period of a 25-day subchronic exposure. (C) 2007 Elsevier B.V. All rights reserved.
Acute Effects of Fenthion on Certain Oxidative Stress Biomarkers in Various Tissues of Frogs (Rana Ridibunda)
(Sage Publications inc, 2012) Kanter, Ahmet; Celik, Ismail
This study was aimed mainly to assess the effects of fenthion on certain oxidative stress biomarkers in various tissues of frogs (Rana ridibunda). Biomarkers selected for stress monitoring were malondialdehyde (MDA) and antioxidant defense system (ADS) such as reduced glutathione (GSH) level, glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), and superoxide dismutase (SOD) activities in the liver, kidney, heart, and brain of frogs exposed to 10 and 20 ppm dosages of fenthion for 24, 48, 72, and 96 h. The results demonstrate an increase in MDA levels in selected tissues following exposure to both concentrations of fenthion. The ADS, GSH-Px, GST, SOD activities and GSH levels also fluctuated after 24, 48, 72, and 96 h in all the treatment groups compared with controls. From the evidence obtained here, it is concluded that the exposure of frogs to fenthion induced an increase in MDA combined with fluctuated ADS. This may reflect the potential role of these parameters as useful biomarkers for oxidative stress in amphibian species.
Acute Effects of Methyl Parathion and Diazinon as Inducers for Oxidative Stress on Certain Biomarkers in Various Tissues of Rainbowtrout (Oncorhynchus Mykiss)
(Academic Press inc Elsevier Science, 2008) Isik, Ismail; Celik, Ismail
Present study aimed mainly to assess oxidative stress pesticides such as methyl parathion (MP) and diazinon, which are widely used insecticides and contaminate aquatic ecosystems, on certain biomarkers in various tissues of rainbowtrout (Oncorhynchcus mykiss). Biomarkers selected for stress monitoring were malondialdehyde (MDA) and antioxidant defense system (ADS) mainly reduced glutathione (GSH), glutathione reductase (GR), peroxidase (GSH-PX), transferase (GST) and superoxidedismutase (SOD) activities in the liver, gills and muscle of fishes exposed to 0.5 and 1 ppm dosages of MP and diazinon for 24,48 and 72 h. According to these results, after the administrations of MP and diazinon promote MDA content in some of the tissues of fishes treated with both dosages of MP and diazinon. With regard to the ADS, GSH-Px, GST, SOD, GR activities and GSH levels fluctuated after 24, 48 and 72 h in all the treatment groups compared with controls. Collective results demonstrated that exposure of fish to pesticides induced an increase in MDA joined with fluctuated ADS. This may reflect the potential role of these parameters as useful biomarkers for assessment of water pollution. (C) 2008 Elsevier Inc. All rights reserved.
Alıç Meyvesi Liyofilize Ekstraktının Sıçanlarda Karbon Tetraklorür Toksisitesine Karşı Antioksidan ve Koruyucu Rolünün Değerlendirilmesi
(2021) Altınbaşak, Murat; Celik, Ismail
Bu makale, karbon tetraklorür (CCl4) toksisitesine karşı alıç (Crataegus orientalis L.) meyve liyofilize ekstrakt (LEH), böbrek ve karaciğer koruyucu potansiyelini ve antioksidan rolünü araştırmayı amaçlamaktadır. Deneysel dizayn; Normal kontrol (NK) CCl4 grubu (0,5 ml / kg CCl4, vücut ağırlığı (wb)), LEH1grubu (100 mg / kg LEH bw), LEH4 grubu (200 mg / kg LEH bw), CCl4+LEH1 grubu (0,5 ml / kg + 100 mg / kg LEH bw) ve CCl4+LEH4 grubu (0,5 ml / kg + 200 mg / kg LEH bw) şeklinde 3 haftalık ekstre muamelesi ile yapılmıştır. Deneysel muamelenin sonunda: LEH'nin böbrek ve karaciğer koruyucu potansiyeli ve antioksidan rolünü, karaciğer ve böbrek hasarı serum biyobelirteçleri, eritrosit, beyin, börek ve karaciğerdeki antioksidan savunma sistemleri bileşenleri (ASSB) ile malondialdehit (MDA) içeriği ölçülerek değerlendirildi. Sonuçlara göre; karaciğer hasarı serum enzim aktiviteleri ve dokuların MDA içeriği CCl4 grubunda NK göre anlamlı olarak daha yüksekti. Bu parametreler, ekstre takviyeli gruplarda CCl4 grubuna kıyasla önemli ölçüde daha düşüktü. Sonuçlara göre, LEH takviyeli diyetinin ASSB'lerdeki dalgalanmaları NK değerlerine doğru parametrelerin çoğunu geri çektiğini göstermektedir. Bu nedenle, CCl4 ile toksisite oluşturulan sıçanlarda alıç bitkisi meyve ekstresinin antioksidan kapasiteye ve karaciğer koruyucu etkilere sahip olabileceği düşünülmektedir.
Antidiabetic Properties of Lyophilized Extract of Acorn (Quercus Brantii Lindl.) on Experimentally Stz-Induced Diabetic Rats
(Elsevier Ireland Ltd, 2015) Dogan, Abdulahad; Celik, Ismail; Kaya, Mehmet Salih
Ethnopharmacological relevance: Acorn obtained from the Quercus brantii Lindl. (QB) tree has been used in Turkish folk medicine. Some studies have reported as an antioxidant, antimicrobial, anti-inflammatory, gastroprotective, antitumoural of QB properties previously, however, its effect on the management of type diabetic 2 and oxidative stress complications is still unexplored. The aims of our study were the evaluation of the protective effect and antioxidant role of acorn lyophilized seed plant extract against STZ-induced diabetic complications as oxidative stress, hepatotoxicity and nephropathy, lipidemia and serum biomarkers of diabetes (SBD). Materials and methods: In-vivo studies were performed on STZ-induced diabetic rats Experiment was designed as I [Normal Control (NC)], II [Diabetes mellitus (DM)], III [DM+Acarbose (20 mg/kg b.w) (DM+AC-20)], IV [DM+ QB (100 mg/kg b.w) (DM+QB-100)], V [DM+QB (250 mg/kg b.w) (DM+ QB-250)] and VI [DM+QB (500 mg/kg b.w) (DM+QB-500)] groups. Results: This study showed that the biochemical analysis showed a considerable increase in the HRDB, DB, LP, MDA and fluctuated ADSC in the II group as compared to that of control group whereas, AC and the plant lyophilized seed plant extract supplementations diet restored the STZ-induced diabetic complications towards the control. alpha-glycosidase activity in DM group showed statistically significant increase with respect to control group in small intestine. Moreover, in accordance with the effects of seed extract; in diabetic rat groups to whom acorn seed extract and acarbose were given, the levels of almost all the concerned parameters were reached to the ones measured at control group. As a result, it was concluded that acorn seed extract had certain healing effects on many complications caused by diabetes. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
Antioxidant and Hepatoprotective Properties of Dried Fig Against Oxidative Stress and Hepatotoxicity in Rats
(Elsevier, 2016) Turan, Abdullah; Celik, Ismail
The aim of this study was to investigate the hepatoprotective effect and antioxidant role of dried fig (DF) (Ficus carica L.) against ethanol-induced oxidative stress. Experiment was designed as normal Control, 20% ethanol, 10% DF and 10% DF+ 20% ethanol groups. The hepatoprotective and antioxidant role of the dried DF supplementation feed against ethanol induced oxidatif stress were evaluated by liver histopathological changes, measuring liver damage serum enzymes (LOSE), antioxidant defense system (ADS) and malondialdehyde (MDA) content in various tissues of rats following the exposure of experimental for 50 days. The biochemical analysis showed a considerable increase the LDSE in the ethanol group as compared to that of control group whereas, decreased in 10% DF +20% ethanol group as compared to that of ethanol group. In addition, the DF supplementation diet restored the ethanol-induced MDA and ADS towards to control. The hepatoprotection of DF is further substantiated by the almost normal histologic findings of liver in 10% DF+ 20% ethanol group against degenerative changes in ethanol group. The results indicated that the DF could be as important as diet-derived antioxidants and antihepatotoxicity in preventing oxidative damage in the tissues by inhibiting the production of ethanol-induced free radicals and hepatotoxicity in rats. (C) 2016 Elsevier B.V. All rights reserved.
Antioxidant and Immune Potential Marker Enzymes Assessment in the Various Tissues of Rats Exposed To Indoleacetic Acid and Kinetin: a Drinking Water Study
(Academic Press inc Elsevier Science, 2006) Celik, Ismail; Tuluce, Yasin; Turker, Musa
In the present study, the influence of two different PGRs, indoleacetic acid (IAA) and kinetin (Kn) on immune potential enzymes, adenosine deaminase (ADA) and myeloperoxidase (MPO), and antioxidant defense enzymes such as glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD) in various tissues of rats were investigated during the treatment as a drinking water model. 100 ppm of IAA and Kn as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 21 days continuously. The PGRs treatments caused different effects on the immune potential and antioxidant defense enzymes of experimented rats compared to controls. Results show that IAA caused a significant decrease in GR activity in the lungs and liver and an increase in the spleen. Also, IAA caused a significant decline in GPx activity in the lungs and an increase in the heart. SOD was significantly reduced in the heart, while increased in the lungs. Furthermore, IAA caused a significant decrease in ADA activity in the heart and blood whereas an increase in the kidney and spleen. MPO activity was also significantly increased in the heart by IAA treatment. The activity of enzymes were also seriously affected by Kn; GR activity decreased in the lungs, brain, and blood while GPx activity decreased in the spleen, brain, and heart. ADA activity was also significantly reduced in the blood whereas MPO activity rose in the spleen. In addition, SOD activity lowered in all tissues except for lungs where a significant increment was determined. As a conclusion, the results indicate that PGRs might affect on antioxidant and immune potential enzymes. These data, along with the determined changes suggest that PGRs produced substantial systemic organ toxicity in the erythrocyte, liver, brain, heart, lungs, spleen, and kidney during the period of a 21-day subacute exposure. (c) 2006 Elsevier Inc. All rights reserved.
The Antioxidant Role of Sideritis Caesarea Infusion Against Tca Toxicity in Rats
(Cambridge Univ Press, 2011) Celik, Ismail; Kaya, Mehmet Salih
Sideritis caesarea (SC) Duman, Aytac&Baser is a member of the Lamiaceae family. The present study was designed to investigate the antioxidant properties of the aerial parts of island green tea SC against TCA effects in rats. Biomarkers selected for monitoring antioxidant capacity were the activities of glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase, catalase (CAT), GSH level and malondialdehyde (MDA) content in various organs of rats. Three experimental groups, A (untreated - control), B (only TCA-treated) and C (TCA + SC-treated), were studied. At the end of the 50 d experiment, the MDA content in tissues increased significantly in group B, whereas no significant changes were observed in group C as compared with that of the control group. Antioxidant enzyme activities such as SOD and CAT increased significantly in the brain, liver and kidneys of group B but decreased significantly in group C as compared with group B. The GSH level and GR activity increased significantly in the brain and liver of group C as compared with the control and TCA-exposed rats. Hence, the study reveals that the constituents present in SC impart protection against chemical-induced oxidative injury that may result in the development of cancer.
Antiproliferative Activity of Malus Sylvestris Miller Against Hepg2 Cell Line With Their Antioxidant Properties and Phenolic Composition
(Walter de Gruyter Gmbh, 2023) Vuran, Nese Eray; Kartal, Deniz Irtem; Celik, Ismail
Objectives: The purpose of this study was to investigate the in vitro antiproliferative activities of Malus sylvestris Miller (MS) ethanolic and water extracts against HepG2 cells together with their antioxidant and phytochemical profiles.Methods: The extracts' antioxidant capacity was assessed by DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) methods and the total phenolic and flavonoid contents were measured spec-trophotometrically. The antiproliferative activities of ex -tracts were tested via XTT colorimetric assay. The phenolic compounds in the extracts were analyzed using HPLC-DAD analysis.Results: The most abundant phenolic compounds in both extract types were rutin, chlorogenic and gallic acid. In parallel to the phenolic composition, the ethanolic extracts showed the higher antioxidant activity, total phenolic and flavonoid content than the water extracts. The ethanolic extracts displayed 4.5-fold more antiproliferative activity than the water extracts against HepG2 cells. The IC50 values for the ethanolic and water extracts of MS were 0.86 and 3.95 mg/mL, respectively.Conclusions: These results contributed to the understanding of the cytotoxic potential of MS. Since wild apple exhibited strong cytotoxic activity against HepG2 cells, it may be used as a natural agent to treat cancer and the further studies are needed to reveal the effects of wild apple on cancer.
Bazı Bitki Büyüme Düzenleyicilerin Subkronik Uygulamasının Sıçanlarda Hematolojik ve Biyokimyasal Parametreler Üzerine Etkileri
(2003) Özbek, Hanefi; Tülüce, Yasin; Celik, Ismail
Bazı bitki büyüme düzenleyicilerinin subletal konsantrasyonda sıçanların hematolojik ve biyokimyasal parametreleri üzerine etkileri laboratuar şartlarında araştırıldı. Sekizer adet dişi sıçandan oluşan gruplar deneme boyunca içebildiğince 100 ppm'lik indolasetik asit (IAA), indolbütirikasit (IBA) ve kinetin uygulamasına üç hafta maruz bırakıldı. Sonuçlar, bu bitki büyüme düzenleyicilerinin kanın hematolojik ve biyokimyasal parametreleri üzerine farklı etkilere sahip olduklarını göstermiştir. Sonuçlara göre, IAA alyuvar (KKH), akyuvar (BKH), trombosit (KP) sayısını ve hemoglobin (Hb) miktarını önemli derecede artırmıştır. IBA uygulaması alyuvar, akyuvar ve trombosit sayısını arttırırken, ortalama hücre hacmini (OHH) ve ortalama hücresel hemoglobin (OHHb) düzeyini azaltmıştır. Akyuvar, trombosit ve hücresel hemoglobin konsantrasyonu (HHbK) kinetin uygulaması ile önemli oranda artmıştır. Biyokimyasal özellikler ile ilgili olarak, IAA total protein miktarını önemli derecede arttırırken, trigliserit ve çok düşük dansiteli lipoprotein seviyelerini (VLDL) azaltmıştır. Diğer yandan, IBA uygulaması albümin oranını arttırdı, ancak trigliserit, kolesterol, total bilirubin ve direkt bilirubin seviyelerini azaltmıştır. Kinetin ise biyokimyasal parametreler üzerinde etkisiz bulunmuştur. Sonuç olarak, bu kimyasalların subkronik uygulamalarda etkili oldukları görülmüştür.
Bir Demir Kaynağı Olarak Sahra Çölü Tozunun Kanatlı Hayvan Verim Özellikleri Üzerine Etkisi
(2001) Demirel, Murat; Saydam, A. Cemal; Celik, Ismail; Söğüt, Bünyamin
Bu çalışmada, Sahra Çölü Tozu' nun kanatlı verim özelliklerine etkisini belirlemek amacıyla bıldırcın kullanılmıştır. Litresinde 120 g Sahra Çölü Tozu bulunan su ışık muamelesinden sonra püskürtme ve içirme şeklinde uygulanmıştır. Muamele uygulaması hayvanların ne kan demir seviyelerine ne de büyüme- gelişme ve karkas özellikleri üzerine olumlu etki etmediği gözlenmiştir. Kan demir seviyesi ile karkas randımanı arasında negatif ve önemli (r = -0,5772; P<0,01), ciğer ağırlığı arasında ise pozitif ve önemli ( r = 0,4642; P<0,05) ilişki saptanmıştır. Diğer taraftan, ansature demir bağlama kapasitesi ile karkas randımanı arasında pozitif ve önemli (r = 0,6011; P<0,01), ansature demir bağlama kapasitesi ile ciğer ağırlığı arasında negatif ve önemli derecede ilişki gözlenmiştir (r = -0,504; P<0,01)
Characterisation of Carbonic Anhydrase Purified From Erythrocytes of Van Cat (Felis Catus)
(Parlar Scientific Publications (p S P), 2013) Soyler, Muhammed; Demir, Halit; Celikezen, Fatih Caglar; Celik, Ismail; Akan, Zafer
Carbonic anhydrase plays different roles in various tissues. It is a key enzyme that regulates the acid-base homeostasis under both normal and pathological conditions. In this study, carbonic anhydrase (CA; E.C 4.2.1.1) was purified from erythrocytes of Van cat (Fells catus) by affinity chromatography. The purification rate was found to be 367.21-fold. SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was used to control the purity of enzyme. Optimal pH, ionic strength, and temperature for enzyme activity were determined. Molecular weight of CA was identified to be about 30 kDa by SDS-PAGE. Optimal pH and temperature were 8.0 and 40 degrees C. The highest activity was found at a concentration of 0.12 M (NH4)(2)SO4 as ionic strength. The Van cat (Fells catus) is a unique and endemic species; therefore, the characterisations of carbonic anhydrase enzyme are crucial. This study will shed light to future studies regarding the Van cat.
Consumption of the Syrian Mesquite Plant (Prosopis Farcta) Fruit and Seed Lyophilized Extracts May Have Both Protective and Toxic Effects in Stz-Induced Diabetic Rats
(Taylor & Francis Ltd, 2022) Agirman, Esvet; Celik, Ismail; Dogan, Abdulahad
The aim of study was to investigate the antidiabetic and antioxidant properties of extracts obtained from dried Prosopis farcta fruit and seeds against streptozotocin-induced diabetes in rats. According to the results, glucose, haemoglobin A1c, alpha-glycosidase activity, liver and kidney damage biomarkers, and malondialdehyde contents of all of the diabetic groups were found to have increased significantly according to the control group. Furthermore, the insulin and C-peptide secretions increased, and liver malondialdehyde level decreased, which were determined as the result of fluctuations in the antioxidant enzyme activities with a dose of 400 mg/kg fruit extract, while seed extract dosages of 100 and 400 mg/kg caused an increase in hepatic demage biomarkers. It was concluded that fruit extract may have insulin secretion stimulating and lipid peroxidation inhibitory effects, whereas seed extract might have caused hepatocyte damage changes to the transport functions and membrane permeability of these cells, thus causing enzymes to leak.
Deneysel Hipertiroidizm Oluşturulan Tavşanlarda Keratin Kinaz ve Kalb Kası Kreatin Kinaz Değerleleri
(1998) Köylü, Halis; Kara, Mehmet; Celik, Ismail; Yeğin, Eşref
Bu çalısmada canlı agırlıkları 1500+50 g olan 20 erkek, beyaz, Yeni Zelanda ırkı tavsan (Oryrctolagus cuniculus huxley) kullanıldı. Tavsanlardan 10 tanesine 15 gün süreyle hergün fizyolojik su (0.15 mo1/1) içinde 50 μg L-tiroksin/100 g, kulak veninden enjekte edilerek hipertiroidizm (tirotoksikoz) olusturuldu. Kontrol grubu olarak ayrılan diger 10 tavsana, aynı hacimde, tiroksinin çözüldügü % 0.9'luk tuzlu su verildi. Çalısma sonunda, total kreatin kinaz (CK) ve kalp kreatin kinaz (CK-MB) degerleri ise sırasıyla, kontrol grubunda 1073 ± 597 U/L ve 874 ± U/L, hipertiroidizm grubunda 3558 ± 1341 U/L ve 902 ± 367 U/L idi. Yapılan istatiksel analiz sonucu, plazma CK degerinin önemli oranda (P<0.001) yükseldigi, CK-MB artısının ise, anlamlı olmadıgı (P>0.05) görüldü. Ayrıca, hipertiroidizm gurubunda CK-Triiyodotronin (T3) (r=0.76, P<0.02) ve CK-Tiroksin (T4) (r=0.64, P<0.05) arasında anlamlı bir korelasyon bulundu. Sonuç olarak; plazma total CK enzim ölçümünün tiroid hastalıklarının tanı ve izlenmesinde yararlı olabilecegi kanaatine varıldı.
Determination Hematotoxic and Hepatotoxic Effects of Trichloroacetic Acid at Sublethal Dosage in Rats
(Pergamon-elsevier Science Ltd, 2009) Celik, Ismail; Temur, Atilla
The present study was designed to understand the effects of sublethal concentration of trichloroacetic acid (TCA) on hematological constituents [Red Blood Corpuscles (RBC) White Blood Corpuscles (WBC), Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Platelet (PLT) counts, Hemoglobin (HB) and Hematocrite (HCT) levels] and liver damage biomarker [cholesterol (CHL), protein (PRT) and bilirubin (BIL)] in rats at subchronic period under laboratory conditions. 2000 ppm dosage of TCA was administered orally to 6 male rats ad libitum during the tests for 52 days consecutively. Results showed that TCA caused a significant increase in the serum BIL whereas decrease in PRT and CHL With regards to hematological constituents, while RBC, MCV, MCH, MCHC, HB and HCT level decreased significantly. PLT counts increased in rats treated with 2000ppm of TCA exposure. The observations presented led us to conclude that TCA administration of subchronic at sublethal dosage induced the liver damage and possessed hematotoxic in rat. These data, along with the determined changes suggest that TCA produce substantial systemic organ toxicity in rats during the period of a 52-day subchronic exposure. (C) 2009 Elsevier Ltd. All rights reserved.
Determination of Chemopreventive Role of Foeniculum Vulgare and Salvia Officinalis Infusion on Trichloroacetic Acid-Induced Increased Serum Marker Enzymes Lipid Peroxidation and Antioxidative Defense Systems in Rats
(Taylor & Francis Ltd, 2008) Celik, Ismail; Isik, Ismail
Today's world is increasingly seeking ways to replace the synthetic drugs with the therapeutic power of natural products. This study was designed to investigate the protective effects of Foeniculum vulgare (FV) and Salvia officinalis (SO) waters infusions against carcinogen chemical trichloroacetic acid (TCA)-exposure in rats. The chemopreventive potential of the plant infusions were evaluated by measuring levels of serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), creatine phosphokinase (CPK), acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] and lipid peroxidation level (Malondialdehyde = MDA) in various tissues of rats. Female Sprague-Dawley rats, weighing 150-200 g, were randomly allotted into four experimental groups. While the control group (A) received only natural spring water, the treatment B group (0.2% TCA) supplied with the drinking water containing 0.2% TCA, the treatment C (TCA + FV infusion) and D (TCA + SO infusion) groups drank the drinking water containing 0.2% TCA and 2.5% the plant grains and leaves ad libitum for 50 days during experiment. At the end of the 50 days experiment, TCA and the plant's infusions caused different affect on the serum marker enzymes, tissues antioxidant defense systems and lipid peroxidation against TCA-exposed in rats with comparison to those of TCA exposed and control rats. According to the results, both TCA and TCA + plants infusions caused a significant increase in serum AST, ALT and CPK activity. Non-enzymic antioxidant GSH level significantly increased in the brain whereas reduced in the erythrocytes and kidney of TCA + FV and TCA + SO as compared to TCA group and control. While MDA content slightly increased in tissues of TCA group in comparison to those of control, significantly decreased in the brain, liver and kidney of rats of TCA + FV and TCA + SO groups as compared to TCA group and control. Antioxidative enzyme activity such as CAT and SOD significantly increased in the brain, liver and kidney tissues of TCA induced group whereas reduced the same enzymes activities as compared to TCA group. The ancillary enzyme GR activity significantly depleted in the brain and kidney of TCA + FV and TCA + SO groups in comparison to those of TCA exposed and control rats. In addition, the drug metabolizing enzyme GST activity significantly declined in the brain and kidney of TCA + FV and TCA + SO groups in comparison to those of TCA exposed and control rats, whereas, also reduced in the liver of TCA + FV and TCA + SO groups in comparison to those of TCA exposed rats. It was concluded that the levels of serum marker enzymes were found not to be decreased in plants treated groups due to hepatic damage induced by TCA. Also the four antioxidant enzymes were found to be activated in different degrees following TCA treatment and declined the activation of the enzymes the plant infusions accompanied by significant reduction in MDA concentration in the tissues. The observations, along with changes, might suggest that the both FV and SO may possess antioxidant properties during the period of a 50-day protective exposure.
Determination of Hepatoprotective and Antioxidant Role of Walnuts Against Ethanol-Induced Oxidative Stress in Rats
(Humana Press inc, 2015) Bati, Bedia; Celik, Ismail; Dogan, Abdulahad
The aims of our study were the evaluation of the hepatoprotective effect and antioxidant role of walnuts against ethanol-induced oxidative stress. The hepatoprotective and antioxidant role of the walnuts supplementation feed against ethanol-induced oxidative stress were evaluated by measuring liver damage serum marker enzymes; aspartate aminotransferase (AST); alanine aminotransferase (ALT); gamma glutamyl transpeptidase (GGT); lactate dehydrogenase (LDH); and antioxidant defense systems such as reduced glutathione, glutathione reductase, superoxide dismutase, glutathione-S-transferase, catalase, and glutathione peroxidase and Malondialdehyde (MDA) content in various tissues of rats. Rats were divided into six experimental groups: I (control), II (20 % ethanol), III (10 % walnuts), IV (20 % ethanol + 10 % walnuts), V (5 % walnuts), and VI (20 % ethanol + 5 % walnuts). According to the results, the biochemical analysis showed a considerable increase in the serum aspartate AST, ALT, GGT, and LDH in the group II as compared to that of group I, whereas decreased in group IVas compared to that of group II. In addition, administration of walnuts supplementation restored the ethanol-induced imbalance between MDA and fluctuated antioxidant system toward close control group particularly in the tissues. The results indicated that walnuts could be as important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the lipid oxidation or inhibiting the production of ethanol-induced free radicals in rats.
Determination of Toxicity of Subacute Treatment of Some Plant Growth Regulators on Rats
(Wiley, 2007) Celik, Ismail; Tuluce, Yasin
The effects of some plant growth regulators (PGRs), 2,3,5-triiodobenzoic acid (TIBA), Naphthaleneacetic acid (NAA) and 2,4-Dichlorofenoxyacetic acid (2,4-D), at sublethal concentrations on antioxidant defense system [glutathione peroxiclases (GPx), reduced glutathione (GSH), glutathione reductase (GR), glutathione-S-transferase (GST) and catalase (CAT], immune potential enzymes [adenosine deaminase (ADA) and myeloperoxidase (MPO)], and lipid peroxidation content [Malondialdehyde, (MDA)] were investigated in lung and speen tissues of rats. Sprague-Dawley albino rats were exposed to 0, 50, or 100 ppm (parts per million) TIBA, NAA, or 2,4-D in drinking water ad libitum for 25 days continuously. According to the results, MDA concentration significantly increased in the tissues treated with 100 pprn dosage of NAA or 2,4-D without any change in the tissues of rats treated with both dosage of TIBA. The GSH depletion in the spleen tissue of rats treated with both the dosage of NAA and 2,4-D were found to be significant. Also, GSH level in the spleen was significantly reduced with 100 ppm of 2,4-D and NAA. The activity of antioxidant enzymes were also seriously affected by PGRs; GPx significantly decreased in the lung of rats treated with both dosages of the PGRs, whereas GPx activity in the spleen were significantly increased with 100 ppm dosage of 2,4-D and NAA. On the other hand, CAT activity significantly decreased in the lung of rats treated with both dosages of NAA, 100 ppm of 2,4-D and 50 ppm of TIBA, and also in the spleen treated with 50 ppm NAA and 2,4-D. The ancillary enzyme GR activity significantly decreased in the spleen with both doses of the PGRs, also in the lung treated with both dosages of 2,4-D, 50 ppm of NAA and 100 ppm of TIBA. The drug metabolizing enzyme GST activity significantly reduced in the lung of rats treated with both dosages of the PGRs and also in the spleen treated with 100 ppm dosage of 2,4-D and TIBA and 50 pprn of NAA. Meanwhile, immune potential enzyme MPO activity significantly increased in the spleen of rats treated with both doses of NAA and TIBA whereas ADA activity significantly decreased in the spleen of rats treated with 100 ppm dose of NAA and TIBA. The observations presented led us to conclude that the administrations of subacute NAA, 2,4-D, and TIBA promote MDA content, inhibit the antioxidative defense system and activate or inhibit immune potential enzymes in the rat's spleen and lung tissues. These data suggest that PGRs produced substantial organ toxicity in the lung and spleen during the period of a 25-day subacute exposure. (C) 2007 Wiley Periodicals, Inc.
Determination of Toxicity of Trichloroacetic Acid in Rats
(Academic Press inc Elsevier Science, 2007) Celik, Ismail
This study aims to investigate the effects of the trichloroacetic acid (TCA) on serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), creatine phosphokinase (CPK), acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] and lipid peroxidation content (Malondialdehyde, MDA) in various tissues of rats. TCA (2000 ppm) as drinking water was administered orally to rats (Sprague-Dawley albino) ad libitum for 50 days continuously. TCA treatments caused different effects on the serum marker enzymes, antioxidant defense systems and the MDA content in experimented rats compared to controls. Results showed that TCA caused a significant increase in serum AST, ALT, CPK and ACP activity. The lipid peroxidation end product MDA slightly increased in the erythrocytes, liver and kidney of rats treated with TCA, whereas did not change in the brain. In addition, antioxidant enzyme activity such as CAT and SOD significantly increased in the brain, liver and kidney tissues of TCA induced group whereas the ancillary enzyme GR and the drug metabolizing enzyme GST activity did not significantly change in the all tissues. The observations presented led us to conclude that the administration of subchronic TCA promotes lipid peroxidation content, elevates tissue damage serum marker enzymes and fluctuates in the antioxidative systems in rats. Also the rats resisted to oxidative stress via antioxidant mechanism but the antioxidant mechanism could not prevent the increases in lipid peroxidation in rat's tissues. These data, along with the determined changes suggest that TCA produced substantial systemic organ toxicity in the erythrocyte, liver, brain and kidney during the period of a 50-day subchronic exposure. (C) 2007 Elsevier Inc. All rights reserved.
Effect of Experimental Diabetes Mellitus on Plasma Lactate Deydrogenase and Glutamic Oxaloacetic Transaminase Levels in Rabbits
(2002) Yeğin, Eşref; Celik, Ismail; Odabaşoğlu, Fehmi
Bu çalışmada, diabet 20 erkek, beyaz, Yeni Zelanda ırkı tavşan (Oryctolagus cuniculus huxley) alloxan verilerek (100 mg/kg vucut i.v.) oluşturuldu ve insulin tedavisi yapılmaksızın 16 hafta bekletildi. Plazma enzimlerini ölçümü için kan örnekleri hem diabetik hemde kontrol grublarından alındı. Muameleden sonra laktat dehidrogenaz (LDH) ve glutamik oksalasetik transaminaz (GOT) seviyeleri belirlendi. LDH ve GOT seviyeleri sırasıyla, kontrol grubunda 155,6 ± 41,9 U/L ve 47,0 ± 20,4 U/L iken, diabetli grubunda 286,9 ± 142,9 U/L ve 93,9 ± 62,5 U/L idi. Yapılan istatistiki analiz sonucu, diabetli, plazma LDH değerinin önemli oranda (p < 0,05) yükseldiği ancak GOT yüksekliğinin önemsiz olduğu (p > 0,05) görüldü. Sonuç olarak, LDH diabetes mellitus için bir indikatör olabilirler ancak, diabetes mellitus’un mevcut karakteristik paremetreleri daha iyi birer indikatördürler.