Browsing by Author "Cetin, Sedat"

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Apoptotic and Oxidative Mechanisms in Liver and Kidney Tissues of Sheep With Fluorosis
(Springernature, 2021) Efe, Ugur; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat
This study was planned to determine the molecular basis and causes of damage to the kidney and the liver, which are the most affected tissues in sheep exposed to chronic fluoride. For this purpose, liver and kidney tissues were obtained from sheep with signs of fluorosis in the age range of 4-6 years. The control group consisted of clinically healthy sheep without fluorosis. The apoptotic and oxidative genes expression of target genes was determined using the real qRT-PCR method. According to the control gene (Gapdh) that was detected that in the liver, the apoptotic genes caspase-8, caspase-9, and Bim increased and caspase-3, Bcl-2, and Bak decreased, while in the kidney, caspase-3 and Bax and caspase-8, Bcl-2, Bcl2l-1, Bim, and Bak decreased. According to the 2-Delta Ct values of the oxidative stress genes, it was determined that Cygb, Gstp1, and Ncf1 genes increased significantly in the fluorosis group and Gpx1, sod1, and sod2 genes decreased significantly. In the kidney tissue, Cygb and Gpx1 increased in the fluorosis group, while sod1, sod2, Gstp1, Ncf1 and Ccs, and Nos2 were found to decrease significantly. As a result, it was shown that apoptosis and oxidative mechanisms are activated in the liver and the kidney tissues of sheep with fluorosis and these parameters have an important role in understanding the molecular basis of tissue damage in fluorosis.
April Cetina, Dede, Oto, Yuksek, Bulduk, the Effect of Resveratrol on Serum Protein Fractions in Rats Exposed To Experimental Chronic Fluorosis
(int Soc Fluoride Research, 2022) Cetin, Sedat; Dede, Semiha; Oto, Gokhan; Yuksek, Veysel; Bulduk, Mehmet; Ozdemir, Hulya
Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and alpha-1 and alpha-2 globulin compared to the control group (p < 0.05), these values were significantly increased (p < 0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The 0- and gamma-globulin concentrations were the lowest in the NaF group statistically (p < 0.05). Despite a significant increase (p < 0.05) in these values in the NaF+Res group, compared to the NaF group, they were still lower compared to the control group. The examination of the percentage of serum protein fractions revealed a reduced albumin in the NaF group compared to the control group but the finding was not statistically significant (p > 0.05). The albumin of the NaF+Res group was statistically higher than that of the control group (p < 0.05). No statistical differences were observed in alpha-1 and alpha-2 globulin across the groups. The 0 -globulin of the NaF group was the highest but not statistically higher than that of the control group. The gamma-globulin percentages in all the groups were found to be lower than the levels in the control group. The albumin/globulin (A/G) ratio decreased in the NaF group but was not significantly different than that of the control group. In conclusion, the alterations in the serum protein fractions due to NaF-induced toxicity, especially the alterations in their concentrations, approached values closer to those of the control group with the administration of resveratrol. We concluded that these results are of potential importance in indicating a favorable role for resveratrol use in preventing and treating fluoride toxicity.
The Concentration of Certain Trace Elements in The Wool of Sheep With Fluorosis
(int Soc Fluoride Research, 2020) Cetin, Sedat; Deger, Yeter; Dede, Semiha; Yur, Fatmagul
The aim of this study was to evaluate the trace element levels in the wool of sheep, with and without fluorosis, living in a volcanic area of Turkey. Fifteen Akkaraman sheep with fluorosis in the Agri region, to the north of Lake Van, and 10 Akkaraman sheep without fluorosis in the Van region, to the south of Lake Van in the eastern part of Turkey, were investigated. The urinary fluoride levels were measured with an ion selective electrode. The sheep with fluorosis were identified by clinical examination and the presence of a high urinary fluoride level. The wool samples were obtained from the neck region. The trace element concentrations in these samples were measured using an atomic absorption spectrophotometer. It was determined that in the fluorosis group, compared to the control group, the copper and zinc levels were significantly decreased (p=0.05), and a non-significant decrease (p=0.05) was present for the levels of the nickel, manganese, iron, and cobalt.
Determination of Blood Serum Protein Fractions of Calves With Clinically Diagnosed Pneumonia
(Agricultural Research Communication Centre, 2021) Ekici, Pinar Tanritanir; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia and evaluate the possibility uses of these parameters as clinical diagnostic parameters. Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis. Result: Albumin, alpha 1-globulin percentages and A/G ratio decreased in the patient group (p <= 0.01), other globulin fractions were higher in the patient group (p <= 0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, alpha 1-globulin (p <= 0.05) and A/G ratio (p <= 0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient groups (p <= 0.01). As a result, the serum protein fractions should be evaluated as a useful biochemical blood parameter for diagnosis and follow up of lung diseases especially in calves.
Dna Damage-Induced by Sodium Flouride (Naf) and the Effect of Cholicalciferol
(Tech Science Press, 2020) Yuksek, Veysel; Dede, Semiha; Usta, Ayse; Cetin, Sedat; Taspinar, Mehmet
It is known that the high electronegativity of fluorine affects various soft tissues, especially the bone structure in organisms. Of these tissues are the kidneys, which play an important role in the excretion of fluoride from the body. Fluoride affects many cellular mechanisms. One of these effects is DNA damage. Our study aimed to investigate the likely protective effect of cholecalciferol (vitamin D3) on genomic DNA damage-induced NaF depending on concentration and time. The IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D-3 through MTT assay in NRK-52E kidney cells were determined. DNA damage was significantly increased (p < 0.05) compared to the control group in all groups except for vitamin D-3 It was determined that treatment with NaF together with vitamin D-3 decreased the DNA damage compared to NaF treated groups for 3 and 12 h. NaF combined with vitamin D3 was determined statistically to decrease (p < 0.05) DNA damage compared to NaF treated groups for 24 h. As a result, it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased (p < 0.05) the genomic DNA damage, but NaF treatment together with vitamin D-3 decreased the DNA damage in renal cells depending on the time. It was concluded that vitamin D-3 may be useful in preventing DNA damage caused by NaF.
The Effect of Lycopene on Dna Damage and Repair in Fluoride-Treated Nrk-52e Cell Line
(Springernature, 2021) Cetin, Sedat; Usta, Ayse; Yuksek, Veysel
Exposure of fluorine at toxic concentrations causes serious damage by accumulating in especially bones, kidneys, and other soft tissues. Fluorine at cytotoxic concentrations may cause DNA damage. This study aims to determine the level of DNA damage due to sodium fluoride (NaF) at different hours (3rd, 12th, and 24th hours) and in IC(50)concentrations designated for each hour and reveal the protective effect of lycopene on possible damage. The best enhancer concentrations (1 mu M) of microtitration (MTT) viability test and proliferation of lycopene and IC(50)values of NaF at the 3rd, 12th, and 24th hour were 9600, 5500, and 3200 mu M, respectively. DNA damage significantly increased in all NaF-treated groups in comparison with the control group (p < 0.05). DNA damage due to NaF+LYC application significantly decreased in comparison with the control group (p < 0.05). Lycopene application significantly increased the expression levels of the Ku70 and Ku80 genes which have a part in DNA repair (p < 0.05). The statistical data showed that application of lycopene which is an important antioxidant molecule may be beneficial for decreasing NaF-induced DNA damage. In conclusion, applying lycopene for cytotoxicity due to fluorine in NRK-52E cell line had different effects based on the dosage and time; thus, it can be a potential option for preventing fluorosis-induced toxicity and developing new treatment approaches.
The Effect of Quinoa (Chenopodium Quinoa) on Apoptotic, Autophagic, Antioxidant and Inflammation Markers in Glucocorticoid-Induced Insulin Resistance in Rats
(Springer, 2022) Erfidan, Siber; Dede, Semiha; Usta, Ayse; Yuksek, Veysel; Cetin, Sedat
Background Insulin resistance plays an important role in predicting type 2 diabetes that may develops. This study was planned in order to investigate the beneficial effects of quinoa (Chenopodium quinoa) use in glucocorticoid induced-insulin resistance. Methods and results Forty-two rats were used as the material (experimental) groups: the control group (C), the quinoa-administered group (Q), the insulin resistance-created group (IR), the IR + metformin group (IM), the IR + quinoa for treatment group (IQ) and the quinoa + IR for prophylaxis group (QI). Blood glucose, insulin levels and HOMA-IR were found to be highest (p < 0.05) in the IR group (p < 0.05). Glucose levels decreased significantly with the administration of quinoa and approached the levels of the control, but the insulin levels and the HOMA-IR did not significantly change. It was also observed that other biochemical parameters (ALT, AST, ALP, total cholesterol, total protein, urea and creatinine) changed significantly in the IR group and approached the levels of the control group with the administration of quinoa. Apoptotic (BCL2 5, BAX 9, CAS 3), autophagic (SQSTM1 7, ATG5) and inflammation (IL-1 beta, TNF-alpha) genes were upregulated by 5-11-fold in the IR group. In the groups in which quinoa was administered for treatment and protection, all these genes were found to be upregulated to a lower extent than the IR group. Antioxidant genes (GPX1, SOD1) increased by nine to tenfold in the quinoa groups. Conclusion As a result, after administration of quinoa, it was determined that the glucose level increased due to experimental insulin resistance and the liver and kidney damage indicators decreased. It was determined that quinoa (Chenopodium quinoa) had significant beneficial effects on biochemical parameters and apoptotic, autophagic, antioxidant and inflammatory markers in experimental glucocorticoid-induced insulin resistance.
The Effect of Vitamin E and Selenium Combination in Repairing Fluoride-Induced Dna Damage To Nrk-52e Cells
(Springer, 2020) Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
Prolonged and excessive fluoride exposure can lead to fluorosis. The kidney is one of the organs that are injured mostly due to fluoride-induced damage. Fluoride can induce DNA damage at cytotoxic concentrations. This study aims to determine the extent of NaF-induced DNA damage and to investigate the effect of vitamin E and selenium combination (ES) in preventing and repairing this damage. For this purpose, we administered different combinations of NaF and ES to NRK-52E cells and determined the effective concentrations of ES and the NaF IC(50)values associated with different incubation times (3, 12, and 24 h) by using the MTT assay. The determined quantities of NaF IC(50)in association with time and the NaF IC50+ ES combination were administered to the cells. The extent of DNA damage was determined with the comet assay and the expression levels of the Ku70/80 and PARP-1 genes were determined with the RT-qPCR method. DNA damage significantly increased in all experimental groups compared to the control group (p < 0.05). It was found out that the NaF and ES combination statistically reduced the DNA damage compared to the damage observed in the NaF-treated groups (p < 0.05). Treatment of the ES combination significantly increased the expressions of Ku70 and Ku80 genes involved in DNA repair (p < 0.05). We concluded that vitamin E and selenium can potentially be effective in the repair of fluoride-induced DNA damage based on the results of this in vitro study. Our results may shed light on the prevention of DNA damage associated with fluorosis.
The Effects of Some Minerals on Apoptosis and Dna Damage in Sodium Fluoride-Administered Renal and Osteoblast Cell Lines
(int Soc Fluoride Research, 2019) Cetin, Sedat; Yur, Fatmagul; Taspinar, Mehmet; Yuksek, Veysel
The present study was planned to investigate the effects of some minerals (MgCl2, Na2SeO3, AlCl3 CaCl2) on the expression and translocation of certain apoptotic markers in NaF-administered (at the rate of IC50) rat renal epithelial (NRK-52E) and human osteoblast (hFOB 1.19) cell lines. The NaF IC50 and the non-toxic mineral doses were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For the biochemical analysis, the cells were collected by trypsin treatment following a 24-hour incubation period, the cells were broken up by the freeze/thaw method, and the analysis was conducted. Caspase 9, 8, and 3 levels and gene expression, and M30 and 8-OHdG levels were determined. Target gene DNAs were propagated with the real time-PCR method. In the MTT studies, it was determined that the cell proliferation in rat renal epithelial cells (NRK-52E) treated with NaF+minerals was higher than that of all the NaF-treated groups and in the human osteoblast cells (hFOB 1.19), the cell proliferation was higher than in all the NaF-treated groups except for the MgCl2 group. The reason why the NaF administration in the NRK-52E cells resulted in an average of a 2-fold decrease in caspase 3 expression compared to the control group could be attributed to the apoptotic effect of NaF based on the time we obtained the RNA. However, based on this time, when the results are assessed based on the NaF and other mineral groups, the NaF-induced cytotoxic apoptosis might have used a pathway other than the apoptotic pathway. Thus, it is considered that minerals could usually prevent NaF-induced apoptosis by a synergistic mechanism due to the ionic character of NaF. NaF+mineral administration protected the NRK-52E cells from apoptosis. In the osteoblasts, on the other hand, it was concluded that NaF+mineral administration may be useful since it inhibits increased apoptosis.
The Effects of Thymoquinone on Dna Damage, Apoptosis and Oxidative Stress in an Osteoblast Cell Line Exposed To Ionizing Radiation
(Taylor & Francis Ltd, 2021) Yilmaz, Osman; Yuksek, Veysel; Cetin, Sedat; Dede, Semiha; Tugrul, Taylan
Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell death, etc.) in living organisms by affecting them directly and indirectly in a time- and dose-dependent manner. This study was planned to present the effects of thymoquinone (TQ) in preventing oxidative stress, DNA damage and apoptotic cell death that may occur after IR exposure. For this purpose, hFOB 1.19 osteoblast cells were used. Using the MTT viability test, the dose of IR reducing the proliferation of cells and the concentration of TQ increasing cell proliferation the most were determined. Five groups were formed as the Control (C), Thymoquinone (TQ), Ionizing radiation (IR), Ionizing radiation + Thymoquinone (IR + TQ) and Thymoquinone + Ionizing radiation (TQ + IR) groups. By applying the determined concentrations on the cells, DNA damage level was determined with the Comet assay, and the gene expression levels of enzymes effective in the oxidative and apoptotic mechanisms were determined with the RT-qPCR method expression. It was determined that, as a result of IR application for 24 and 72 h, DNA damage occurred in the osteoblast cell line. While no significant change was observed in the oxidative and apoptotic gene expression levels at the end of 24 h, an increase in comparison to the control group was determined in the Sod-1 (2.4-fold), Caspase-3, and Caspase-9 gene expression levels at the end of 72h. . Application of TQ in 72 h of incubation before and after IR application significantly reduced the expression levels of Caspase-3, Caspase-9, and DNA damage together with the increased GPx-3 and Sod-1 gene expression.Consequently, it was seen that IR application affected apoptotic and oxidative stress-regulating genes in the hFOB 1.19 osteoblast cell line in a time- and dose-dependent manner, and it was thought that the harmful effects on this cell line may have been caused by the activation of the mitochondrial pathway. It was determined that TQ significantly reduced the gene expression values that changed due to IR and brought them close to those of the control group, and it was concluded that TQ became useful by showing antioxidant properties against the harmful effects of IR.
The Effects of Vitamin D Application on Naf-Induced Cytotoxicity in Osteoblast Cells (Hfob 1.19)
(Springernature, 2023) Dede, Semiha; Taspinar, Mehmet; Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to fluoride exposure in vitro. NaF (IC50) and vitamin D (proliferative) were applied to human osteoblast (hFOB 1.19) cells. The major genes of apoptotic, autophagic, and necrotic pathways were determined by RT-PCR. 2-Delta Delta Ct formulation was used for expression analysis. In the NaF group, caspase 3, Bax, Bad, Bak, Bclx, Atg3, Atg5, Atg6, pG2, LC3-I, LC3-II, RIP1, and RIP3 genes were increased (2.6-15 times). It was observed that the expressions of these genes approached the control when vitamin D was given together with NaF. The Bcl2 gene increased significantly (sixfold) with the effect of NaF, and was down-regulated to some extent with additional vitamin D administration, but still more than in the control. As a result, it was determined that apoptotic, necrotic, and autophagic pathways were activated as the molecular basis of the damage in the bone tissue, which was most affected by fluorine, and these genes were down-regulated and approached the control group with the addition of vitamin D. It was concluded that this is an important data to explain the molecular basis of the protective and therapeutic effect of vitamin D against fluorine toxicity.
The Effects of Vitamins A, D, E, and C on Apoptosis and Dna Damage in Sodium Fluoride-Treated Renal and Osteoblast Cell Lines
(int Soc Fluoride Research, 2017) Yuksek, Veysel; Dede, Semiha; Taspinar, Mehmet; Cetin, Sedat
This study was planned to investigate the effects of the antioxidant and protective vitamins A, D, E, and C, on the expression and translation of certain apoptotic markers in the NRK-52E and hFOB 1.19 cell lines treated with NaF at half the maximal inhibitory concentration (IC50) for 24 hours. The IC50 for NaF and nontoxic vitamin doses were determined by the MTT viability test. For the biochemical assays, cells were harvested by trypsinization and lysed by the freeze/thaw method. The levels and gene expression of caspases 3, 8, and 9, and the levels of M30 and 8-OHdG were also measured with methods that included the use of ELISA and qRT-PCR. In the MTT studies, compared to the NaF-treated groups, it was found that the cell viability was higher in all the NaF+vitamin D-treated groups in the NRK-52E cell line, in some of the NaF+vitamin D-treated groups in the hFOB 1.19 cell line, and in some of the NaF+vitamin A, E, and C-treated groups for both cell lines. In the NRK-52E cell line, the NaF IC50 value was determined and found not to induce apoptosis sufficiently so that it was considered that mechanisms other than the apoptotic pathways were instrumental in causing cell death. In the hFOB 1.19 cell line, it was observed that the apoptotic M30 protein level was increased in the NaF+vitamin D and NaF+vitamin C groups. In addition, in the hFOB 1.19 cell line, the qRT-PCR results showed that, while the expression of caspase-3 increased with vitamin A and that of caspase-8 increased with NaF, treatment with NaF+vitamin A led to a lower levels of caspases 3 and 8. Future studies to investigate the most valid and active mechanism for NaF-induced cell death and to elucidate the inhibitory-activating effects of vitamins on this mechanism using different doses, durations of exposure, and analytic methods should be considered.
Exploring the Combined Anti-Cancer Effects of Sodium Butyrate and Celastrol in Glioblastoma Cell Lines: a Novel Therapeutic Approach
(Humana Press inc, 2024) Kartal, Bahar; Denizler Ebiri, Farika Nur; Gueven, Mustafa; Taspinar, Filiz; Canpinar, Hande; Cetin, Sedat; Taspinar, Mehmet
Glioblastoma, a highly aggressive and lethal brain cancer, lacks effective treatment options and has a poor prognosis. In our study, we explored the potential anti-cancer effects of sodium butyrate (SB) and celastrol (CEL) in two glioblastoma cell lines. SB, a histone deacetylase inhibitor, and CEL, derived from the tripterygium wilfordii plant, act as mTOR and proteasome inhibitors. Both can cross the blood-brain barrier, and they exhibit chemo- and radiosensitive properties in various cancer models. GB cell lines LN-405 and T98G were treated with SB and CEL. Cell viability was assessed by MTT assay and IC50 values were obtained. Gene expression of DNA repair, apoptosis, and autophagy-related genes was analyzed by RT-PCR. Cell cycle distribution was determined using flow cytometry. Viability assays using MTT assay revealed IC50 values of 26 mM and 22.7 mM for SB and 6.77 mu M, and 9.11 mu M for CEL in LN-405 and T98G cells, respectively. Furthermore, we examined the expression levels of DNA repair genes (MGMT, MLH-1, MSH-2, MSH-6), apoptosis genes (caspase-3, caspase-8, caspase-9), and an autophagy gene (ATG-6) using real-time polymerase chain reaction. Additionally, flow cytometry analysis revealed alterations in cell cycle distribution following treatment with SB, CEL and their combination. These findings indicate that SB and CEL may act through multiple mechanisms, including DNA repair inhibition, apoptosis induction, and autophagy modulation, to exert their anti-cancer effects in glioblastoma cells. This is the first study providing novel insights into the potential therapeutic effects of SB and CEL in glioblastoma.
In Vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative Dna Damage in High-Glucose Condition
(Wolters Kluwer Medknow Publications, 2019) Bazyel, Bunyamin; Dede, Semiha; Cetin, Sedat; Yuksek, Veysel; Taspinar, Mehmet
Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 mM), L2 (20 mM), and L3 (40 mM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P % 0.001), whereas L1 caused a decrease in HG group (P % 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P % 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P % 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P % 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.
In Vitro Evaluation of Thymoquinone and Lycopene Supplementation on Oxidative Dna Damage and Oxidant Status in High Glucose Conditions
(Colegio Farmaceuticos Provincia de Buenos Aires, 2019) Dede, Semiha; Yur, Fatmagul; Taspinar, Mehmet; Cetin, Sedat; Usta, Ayse; Yuksek, Veysel
The present study was planned to investigate the effects of thymoquinone (TQ) and lycopene (LYC), known to possess pro-inflammatory and antioxidant properties, on oxidative DNA damage (8-hydroxy-2-cleoxyguanosine) in BHK-21 cell line treated with high glucose (FIG) and the antioxidant system. BHK-21 cell line was cultured with regular passages (5% FBS, 10% host serum, 1% L-glutamine, 1% penicillin/streptomycin - RPMI 1640, 5% CO2 and 95%, 37 degrees C incubation). MTT cell viability tests were conducted. Proliferative TQ and LYC and glucose IC50 values were determined. Control, study groups; glucose (285 mM), TQ (10 mu M), and LYC (50 mu M)) and cross groups were designed. After incubation, trypsinized cells were broken by the freeze/thaw method and analyzed. Oxidative DNA damage, TAS, TOS and OSI values were determined for the obtained samples. It was determined that 8-OHdG levels were affected by high glucose (p <= 0.05), they increased further with the administration of TQ and LYC in addition to HG. TOS and OSI values increased in all study groups when compared to the control (p <= 0.05), and TAS levels significantly decreased (p <= 0.05) with the administration of HG when compared to TQ and LYC groups. In conclusion, TQ and LYC administration in addition to high glucose exacerbated oxidative DNA damage and OSI, and decreased TAS when compared to TQ and LYC groups. The TQ and LYC dose and administration duration in addition to high glucose in the present study led to an improvement in oxidative balance in the BHK cell line.
In Vivo Evaluation of Thymoquinone on Apoptosis and Oxidative Dna Damage in High Glucose Condition
(C M B Assoc, 2018) Gumus, Ali Furkan; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat; Taspinar, Mehmet
The study was planned to investigate the effects of thymoquinone (TQ), which is a compound in N. sativa, on caspase dependent apoptosis and oxidative DNA damage in high glucose treated PC12 cells. PC12 cells were treated with high glucose (G1-150 mM, G2-250 mM, G3-350 mM), TQ (20 mu M), and their combinations. Oxidative DNA damage (8-OHdG (8-Oxo-2'-deoxyguanosine)), and apoptosis (caspase 3, caspase 8, caspase 9 enzymes and M30 protein) parameters were analyzed with ELISA. The 8-OHdG levels decreased in all combination groups compared to the control (p <= 0.001). There was no statistically significant difference between caspase 3 and 9. Caspase 8 in TQ, G3, TQG1, TQG2 groups were higher than the control (p <= 0.002). Low M30 levels were observed in TQG1 group (p <= 0.002). In conclusion, it was observed that in PC12 cell line treated with the high glucose concentrations, TQ administration had a statistically significant effect on oxidative DNA damage and some apoptotic parameters (caspase 8 and M30 protein).
Levels of Trace Elements in Muscle and Kidney Tissues of Sheep With Fluorosis
(Humana Press inc, 2016) Cetin, Sedat; Yur, Fatmagul
In this study, we report the concentrations of four trace elements in muscle and kidney tissues of sheep grown in an area with endemic fluorosis. Fifteen 3- to 4-year-old fluorotic sheep were selected for the study. Ten age-matched sheep with no sign of fluorosis were used as the control group. The animals were killed in a slaughterhouse in the village of DogubeyazA +/- t, located in the AgrA +/- province of Eastern Turkey, where kidney and muscle tissue samples were surgically obtained to be analyzed for copper, zinc, nickel, and iron. In muscle tissue of the fluorotic sheep, the copper levels were higher than those of the control group (p < 0.05). In the case of zinc, its levels were significantly higher in the controls than in the sheep with fluorosis (p < 0.01). No statistically significant differences were found in the muscle contents of nickel and zinc (p > 0.05). Compared to controls, the concentrations of zinc (0.01), iron (p < 0.05), and nickel (p < 0.05) were significantly higher in kidney tissues of fluorotic sheep, but there were no significant differences of the copper levels (p > 0.05). These results suggest that fluorosis significantly alters the mineral metabolism in muscle and kidney resulting in higher levels of mineral accumulation and excretion caused by fluoride intoxication. Further research shall focus on the enzymatic and metabolic activities of these and other trace elements.
Lycopene Prevents Cell Death in Nrk-52e Cells by Inhibition of High Glucose-Activated Dna Damage and Apoptotic, Autophagic, and Necrotic Pathways
(Wiley, 2024) Usta, Ayse; Yuksek, Veysel; Cetin, Sedat; Dede, Semiha
This study aims to investigate the effects of lycopene on apoptotic, autophagic, and necrotic pathways, oxidative status, and DNA damage in diabetic nephropathy at the molecular level. The sample of the study includes seven groups: lycopene (L), high glucose (G), high glucose + lycopene (GL), and control (C) groups tested at 12 and 24 h. The expression levels of genes in oxidative, apoptotic, autophagic, and necrotic cell death pathways are determined by reverse transcription-quantitative polymerase chain reaction analysis. The comet assay method is used for the analysis of DNA damage. It is observed that adding lycopene to high glucose for protective purposes reduces the expression of genes related to apoptosis, autophagy, and necrosis, as well as the DNA damage index, compared to cells given high glucose alone. Lycopene can be a safe and effective alternative agent. In this study, where the molecular mechanisms were examined, it was concluded that lycopene, used as an agent of necrosis, autophagy and apoptosis inhibition, had beneficial effects on cells treated with high glucose. image
Vitamin D May Assist the Upr Against Sodium Fluoride-Induced Damage by Reducing Ripk1, Atg5, Becn1, Oxidative Stress and Increasing Caspase-3 in the Osteoblast Mc3t3-E1 Cell Line
(Elsevier Gmbh, 2023) Yuksek, Veysel; Dede, Semiha; Cetin, Sedat; Usta, Ayse; Taspinar, Mehmet
Background: Out of all measure systemic exposure to fluorides can cause defect of skeletal and dental fluorosis. Endoplasmic reticulum (ER) stress is caused by fluorine-induced oxidative stress and importance of vitamin D in its prevention is not known enough in bone cells. This study was carried out to investigate fluorine-induced oxidative stress, ER stress, and death pathways and the effect of vitamin D on them.Methods: MC3T3-E1 mouse osteoblast cell line was used as the material of the study. The NaF and vitamin D concentrations were determined by the MTT assay. NaF treatments and vitamin D supplementation (pre-add, co-add, and post-add) was administered in the cell line at 24th and 48th hours. The expression of the genes in oxidative stress, ER stress, and death pathways was determined using RT-qPCR and Western blotting techniques.Results: Vitamin D significantly reduced mRNA expression levels of SOD2, CYGB, ATF6, PERK, IRE1, ATG5 and BECN1 whereas caused an increase in levels GPX1, SOD1, NOS2 and Caspase-3 in MC3T3-E1 mouse osteoblast cell line of NaF-induced. In addition, GPX1, SOD1, ATF6, PERK, IRE1, BECN1, Caspase-3 and RIPK1 protein levels were examined by Western blot analysis, and it was determined that vitamin D decreased IRE1 and PERK protein levels, but increased GPX1, SOD1, ATF6 and Caspase-3 protein levels.Conclusion: The findings of the study suggest that vitamin D has protective potential against NaF-induced cytotoxicity reasonably through the attenuation of oxidative stress, ER stress, ATG5, IRE1 and by increasesing caspase-3 in vitro conditions.