Browsing by Author "Cinar, D. Ali"

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Effect of Chronic Exposure To Sodium Fluoride and 7,12-Dimethylbenz[a]anthracene on Some Blood Parameters and Hepatic, Renal, and Cardiac Histopathology in Rats
(int Soc Fluoride Research, 2018) Yildirim, Serkan; Ekin, Suat; Huyut, Zubeyir; Oto, Gokhan; Comba, Arzu; Uyar, Hasan; Cinar, D. Ali
This study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker's necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity.
An Investigation of Protective Effects of Litium Borate on Blood and Histopathological Parameters in Acute Cadmium-Induced Rats
(Humana Press inc, 2018) Yildirim, Serkan; Celikezen, Fatih Caglar; Oto, Gokhan; Sengul, Emin; Bulduk, Mehmet; Tasdemir, M.; Cinar, D. Ali
This study was carried out to determine the protective effects of lithium borate (LTB) on blood parameters and histopathological findings in experimentally induced acute cadmium (Cd) toxicity in rats. Twenty-eight male Wistar albino rats were used, weighing 200-220 g, and they were randomly divided into four groups, including one control and the following three experimental groups: a Cd group (0.025 mmol/kg), a LTB group (15 mg/kg/day orally for 5 days), and a LTB + Cd group (15 mg/kg/day orally for 5 days and Cd 0.025 mmol/kg by intraperitoneal injection on the fifth day). All the rats in the study were anesthetized with ketamine at the end of the sixth day, blood was taken from their hearts, and then the rats were decapitated. The values in the control and LTB group were usually close to each other. White blood cell (WBC), neutrophil %, and C-reactive protein (CRP) levels increased in the Cd and LTB + Cd groups while lymphocyte and monocyte levels decreased in a statistically significant manner, in comparison to the other groups. It was determined that the levels of red blood cells (RBCs), hematocrit (Htc), and hemoglobin (Hb) did not change in the groups. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the Cd and LTB + Cd groups significantly increased, in comparison to the other groups, while the glucose, alkaline phosphatase (ALP), albumin (ALB), and total protein (TP) levels decreased. According to histopathological findings in the control and LTB groups, the liver and kidney tissues were found to have normal histological structures. In the Cd group, severe necrotic hemorrhagic hepatitis, mild steatosis, and mononuclear cell infiltration were detected in the liver. In the LTB + Cd group, degeneration and mild mononuclear cell infiltration were found in the liver. Regarding the kidney tissue in the Cd group, severe intertubular hyperemia in both kidney cortex and medulla, as well as degeneration and necrosis in the tubulus epithelium, was observed. In the LTB + Cd group, mild interstitial hyperemia and mononuclear cell infiltration was detected. Resultantly, it can be said that LTB at this dose has non-toxic effects and some beneficial effects for liver and kidney damage caused by acute Cd toxicity.
An Investigation of the Protective Effects of Resveratrol on Some Biochemical Parameters and Histopathological Findings in Experimentally-Induced Chronic Flurosis in Rats
(int Soc Fluoride Research, 2017) Yildirim, Serkan; Oto, Gokhan; Comba, Bahat; Ekin, Suat; Cinar, D. Ali
This study was carried out to determine the protective effects of resveratrol on some biochemical parameters and histopathological findings in experimentally-induced chronic fluorosis in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into four groups of 10 rats and treated for 12 weeks as follows: (i) control group (drinking water=tap water), (ii) resveratrol group (drinking water=50 mg resveratrol/L), (iii) NaF group (drinking water=10 mg NaF/L), and (iv) NaF+resveratrol group (drinking water=10 mg NaF + 50 mg resveratrol/L). The animals were sacrificed at the end of the 12 weeks. The hematological values of the control, resveratrol, NaF, and NaF+resveratrol groups were, respectively: RED BLOOD CELLS: 6.99 +/- 0.35, 6.77 +/- 0.41, 6.60 +/- 0.57, and 6.84 +/- 0.65 x10(6)/mm(3); HEMATOCRIT VALUE: 40.93 +/- 2.47, 43.54 +/- 3.38, 40.67 +/- 3.12, and 40.73 +/- 5.21 %; HEMOGLOBIN CONCENTRATION: 13.74 +/- 0.41, 14.62 +/- 0.37, 13.00 +/- 0.49, and 13.38 +/- 0.52 g/dL; PLATELETS: 369.85 +/- 65.73, 383.67 +/- 71.15, 291.25 +/- 66.51, and 351.83 +/- 71.23 x10(3)/mm(3); VITAMIN E: 1.042 +/- 0.045, 1.060 +/- 0.088, 0.977 +/- 0.070, and 0.998 +/- 0.060 mu mol/L; VITAMIN A: 3.141 +/- 0.107, 3.071 +/- 0.134, 2.555 +/- 0.093, and 2.90 +/- 0.131 mu mol/L; VITAMIN D: 0.653 +/- 0.043, 0.621 +/- 0.039, 0.419 +/- 0.039, and 0.582 +/- 0.046 mu mol/L, and VITAMIN K: 0.778 +/- 0.047, 0.756 +/- 0.068, 0.706 +/- 0.052, and 0.725 +/- 0.053 mu mol/L. The hematocrit hemoglobin levels in the resveratrol group were significantly increased (p<0.05) compared to the other groups. The platelet counts, vitamin A, and vitamin D levels were significantly lower (p<0.05) in the NaF group than in the other groups. The histopathological findings were: (i) in the control and resveratrol groups, the liver and bone tissue were found to have a normal histological structure, (ii) in the NaF group, hydropic degeneration and colangiohepatitis were detected in the liver, (iii) in the NaF+ resveratrol group, liver degeneration and colangiohepatitis were not found in the liver, (iv) in the NaF group, thinning of the bone tissue trabeculae and a significant decrease in the cellular density of the epiphyseal growth plate were observed, (v) in the NaF+ resveratrol group, a slight degree of thinning was detected in the bone trabeculae and the epiphyseal plate. It was concluded that resveratrol has protective effects on some biochemical parameters and histopathological findings in chronic fluorosis.