An Investigation of the Protective Effects of Resveratrol on Some Biochemical Parameters and Histopathological Findings in Experimentally-Induced Chronic Flurosis in Rats

Abstract

This study was carried out to determine the protective effects of resveratrol on some biochemical parameters and histopathological findings in experimentally-induced chronic fluorosis in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into four groups of 10 rats and treated for 12 weeks as follows: (i) control group (drinking water=tap water), (ii) resveratrol group (drinking water=50 mg resveratrol/L), (iii) NaF group (drinking water=10 mg NaF/L), and (iv) NaF+resveratrol group (drinking water=10 mg NaF + 50 mg resveratrol/L). The animals were sacrificed at the end of the 12 weeks. The hematological values of the control, resveratrol, NaF, and NaF+resveratrol groups were, respectively: RED BLOOD CELLS: 6.99 +/- 0.35, 6.77 +/- 0.41, 6.60 +/- 0.57, and 6.84 +/- 0.65 x10(6)/mm(3); HEMATOCRIT VALUE: 40.93 +/- 2.47, 43.54 +/- 3.38, 40.67 +/- 3.12, and 40.73 +/- 5.21 %; HEMOGLOBIN CONCENTRATION: 13.74 +/- 0.41, 14.62 +/- 0.37, 13.00 +/- 0.49, and 13.38 +/- 0.52 g/dL; PLATELETS: 369.85 +/- 65.73, 383.67 +/- 71.15, 291.25 +/- 66.51, and 351.83 +/- 71.23 x10(3)/mm(3); VITAMIN E: 1.042 +/- 0.045, 1.060 +/- 0.088, 0.977 +/- 0.070, and 0.998 +/- 0.060 mu mol/L; VITAMIN A: 3.141 +/- 0.107, 3.071 +/- 0.134, 2.555 +/- 0.093, and 2.90 +/- 0.131 mu mol/L; VITAMIN D: 0.653 +/- 0.043, 0.621 +/- 0.039, 0.419 +/- 0.039, and 0.582 +/- 0.046 mu mol/L, and VITAMIN K: 0.778 +/- 0.047, 0.756 +/- 0.068, 0.706 +/- 0.052, and 0.725 +/- 0.053 mu mol/L. The hematocrit hemoglobin levels in the resveratrol group were significantly increased (p<0.05) compared to the other groups. The platelet counts, vitamin A, and vitamin D levels were significantly lower (p<0.05) in the NaF group than in the other groups. The histopathological findings were: (i) in the control and resveratrol groups, the liver and bone tissue were found to have a normal histological structure, (ii) in the NaF group, hydropic degeneration and colangiohepatitis were detected in the liver, (iii) in the NaF+ resveratrol group, liver degeneration and colangiohepatitis were not found in the liver, (iv) in the NaF group, thinning of the bone tissue trabeculae and a significant decrease in the cellular density of the epiphyseal growth plate were observed, (v) in the NaF+ resveratrol group, a slight degree of thinning was detected in the bone trabeculae and the epiphyseal plate. It was concluded that resveratrol has protective effects on some biochemical parameters and histopathological findings in chronic fluorosis.