Browsing by Author "Citoglu, Guelcin Saltan"

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Analgesic Compounds From Scorzonera Latifolia (Fisch and Mey.) Dc
(Elsevier Ireland Ltd, 2010) Bahadir, Ozlem; Citoglu, Guelcin Saltan; Smejkal, Karel; Dall'Acqua, Stefano; Ozbek, Hanefi; Cvacka, Josef; Zemlicka, Milan
Ethnopharmacological relevance: A traditional mastic named yaki sakizi prepared from the roots of Scorzonera latifolia (Fisch. and Mey.) DC. is used as a folk remedy for treatment of pain in Turkish folk medicine. Aim of the study: To isolate and identify the compounds responsible for the antinociceptive activity of S. latifolia using bioassay-guided fractionation. Materials and methods: The methanolic extract of the S. latifolia roots was prepared and subjected to isolation procedures such as solvent-solvent partitioning and column chromatography. Writhing and tail-flick tests were used to determine the antinociceptive activity. Results: The n-hexane fraction of the S. latifolia root methanolic extract showed potent antinociceptive activity in both writhing and tail-flick tests. Three compounds were isolated from n-hexane fraction using bioassay-guided chromatographic purification. Isolated compounds were the triterpene taraxasteryl myristate, taraxasteryl acetate, and fern-7-en-3-beta-one, structures were elucidated by means of MS and NMR techniques. Both taraxasterol derivatives showed promising antinociceptive activity when compared to reference compounds. Conclusion: Results of the present study support the usage of S. (Walla in Turkish folk medicine. Both plant root extract and the isolated compounds showed promising antinociceptive activities. Our results suggested that antinociceptive activity of the plant extract is probably caused by the synergistic interaction of the isolated compounds. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Evaluation of Anti-Inflammatory Effect of Zosima Absinthifolia and Deltoin
(Academic Journals, 2010) Bahadir, Oezlem; Citoglu, Guelcin Saltan; Ozbek, Hanefi
In this study, n-Hexane extracts prepared from aerial part of Zosima absinthifolia (ZAA) and the root of Z. absinthifolia (ZAR) (Umbelliferae) as well as deltoin were evaluated for their anti-inflammatory activity using the carrageenan-induced rat paw oedema test. The anti-inflammatory activity of Z. absinthifolia was not found to be significantly different at doses of 25 and 50 mg/kg. However, the aerial part of ZAA at 75 mg/kg, i.p., and Deltoin at 10 mg/kg, i.p. showed a significant reduction with 86.56 and 71.05% respectively in rat paw oedema induced by carrageenan against the reference anti-inflammatory drug indomethacin (3 mg/kg, i.p.) (87%). The ED50 of deltoin, ZAA and ZAR were determined as 5.08, 69.82 and 38.51 mg/kg, respectively. Furthermore, content of deltoin and columbianadin in both ZAA and ZAR were also analyzed by HPLC. Deltoin content of plant was found higher than the columbianadin content in ZAA and ZAR. The present study reveals that the aerial part of Z. absinthifolia possesses promising anti-inflammatory activity in rats.