Browsing by Author "Dalar, Abdullah"

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Amino Acid and Dicyclohexylurea Linked Pyrazole Analogues: Synthesis, in Silico and in Vitro Studies
(Wiley-v C H verlag Gmbh, 2023) Cetin, Adnan; Donmez, Ali; Dalar, Abdullah; Bildirici, Ishak
Pancreatic lipase (PL) inhibitors have received considerable attention by several researchers because of its ability to hydrolyse the triglycerides in the small intestine. This study reports the (i) synthesize of new pyrazole derivatives binding amino acid and Dicyclohexylurea (DCU), (ii) their pharmaceutical potentials- via enzyme inhibitory activity towards PL and antioxidant activities (using complementary antioxidant methods including FCR, FRAP and ORAC), (iii) the possible interactions between pyrazole compounds and PL enzyme through in silico studies, and the pharmacokinetic properties of the tetra-substituted pyrazole analogues by PreADMET. Enzyme activities with IC50 values of the pyrazole analogues were found to be in a high range of 6.6 +/- 0.4 mu M to 13.5 +/- 0.2 mu M. However, antioxidant activities of the pyrazole analogues exhibited low binding affinities against FCR, FRAP, and ORAC. The pyrazole analogues with docking scores were in the range of -7.3 to -15.2 and their SAR analysis were demonstrated to highlight the importance of amino acid and DCU linked scaffolds. Two web tools were utilized for the purpose of predicting ADMET parameters of drugs and drug-like pyrazole analogues. These results suggested that the amino acid and DCU linked pyrazole analogues have potential as PL inhibitors.
Analysis of in Vitro Biological Activities and Bioactive Composition of Helichrysum Pallasii (sprengel) Ledeb Plant
(2020) Işık, Nejmi; Dalar, Abdullah
Helichrysum pallasii (Sprengel) Ledeb. Bitkisinin İn Vitro Biyolojik Aktivite ve Biyoaktif Kompozisyon Analizi, Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Eczacılık Bölümü, Temel Eczacılık Bilimleri Anabilim Dalı, Yüksek Lisans Tezi, Van, 2020. Bu çalışma, Van ili ve çevresinde böbrek rahatsızlıkları, karın ağrısı, astım, ishal, sarılık, diyabet gibi çeşitli hastalıkların tedavisinde etkin bir şekilde kullanılan Helichrysum pallasii bitki yaprak organından elde edilen çeşitli ekstrelerin (etanol, infüzyon ve dekoksiyon) in vitro biyolojik aktivite (antioksidan ve enzim inhibisyonu) ve biyoaktif kompozisyonunun incelenmesini amaçlamıştır. Laboratuvar analizleri etanol bazlı ekstrenin geleneksel hazırlanma biçimlerine (infüzyon ve dekoksiyon) göre daha yüksek antioksidan (FCR: 103 mg Gallik asit Eş./g; FRAP: 2205 µmol Fe2+/g; ORAC: 2540 µmol Troloks Eş./g; DPPH: IC50-0.58 mg/ml; CUPRAC: IC50-0.37 mg/ml; Phosphomolybdenum: IC50-1.34 mg/ml ve metal şelatlama: IC50-1.42 mg/ml) ve enzim inhibisyonu (Asetilkolinesteraz: IC50-1.49 mg/ml; Bütirilkolinesteraz: IC50-1.98 mg/ml; Tirozinaz: IC50-0.68 mg/ml; Alfa-amilaz: IC50-2.09 mg/ml; Alfa-glukozidaz: IC50-0.51 mg/ml; ve Pankreatik lipaz: IC50-42.5 µg/ml) aktivitelerine sahip olduğunu ve daha yüksek miktarlarda fenolik (Klorocenik asit izomerleri ve rutin) ve yağ asidi (Palmitik, linoleik ve linolenik asitler) bileşiklerini içerdiğini göstermiştir. Bu çalışmadan elde edilen bulgular, Helichrysum pallasii bitki yapraklarının yüksek antioksidan ve enzim inhibisyonu aktivitelerine sahip zengin bir fitokimyasal kaynak olduğunu göstermekte ve biyoterapötik ajan araştırma ve geliştirme çalışmalarında kullanılabilecek bir aday olarak önermektedir.
Analysis of Phytochemical Composition and Biological Activities of Verbascum Cheiranthifolium Var. Cheiranthifolium Stem and Flowers
(2018) Bengü, Aydın Şükrü; Dalar, Abdullah; Allahverdiyev, Oruc
Within this study phytochemical composition, antioxidant and enzymeinhibitory activities of extracts obtained from stem and flower of Verbascumcheiranthifolium var. cheiranthifolium were analysed. Both of the extracts weredetected as rich sources of phenolics (verbascoside and luteolin hexoside), variousvolatile and fatty acid compounds. Luteolin hexoside rich stem extract hadpronounced FCR, FRAP and α-glucosidase inhibitory activities. Flower extract hadhigh levels of ORAC assay and effectively suppressed activity of pancreatic lipaseenzyme, which was rich in verbascoside compound. Phenolic compounds andvolatile compounds present in the extracts might be the main contributors ofantioxidant capacity and enzyme inhibitory activities of the stem and flowerextracts. Pronounced antioxidant and enzyme inhibitory activities and richbioactive composition determined in this study reveal that Verbascumcheiranthifolium var. cheiranthifolum extracts might be a good source for naturalhealth attributing sources.
Analysis of Phytochemicals and Evaluation of Potential Health Benefits of Plantago Anatolica Tutel & R.r. Mill Plant
(2018) Mükemre, Muzaffer; Ünal, Murat; Dalar, Abdullah
Bu çalışma ile beraber ülkemizin Doğu Anadolu Bölge'sinde yayılış gösteren ve yöre halkı tarafından halk hekimliğinde çeşitli hastalıkların (diyabet, karın ağrısı) tedavisinde yoğun bir biçimde kullanılan endemik bir tıbbi bitki türü olan Plantago anatolica Tutel & R.R. Mill bitkisi farmakobotanik ve farmakognozik açıdan incelenmiştir. Farmakobotanik çalışmalar çiçeklenme ve meyvelenme dönemlerinde toplanan P. anatolica bitki örneklerinin morfolojik ve anatomik açıdan karşılaştırılması aşamalarını kapsamaktadır. Farmakognozik çalışmalar, P. anatolica bitki örneklerinden elde edilen liyofilize ardışık (sırasıyla; n-hekzan, kloroform, etil asetat, aseton, etanol ve saf su), infüzyon, dekoksiyon ve su ekstrelerinin antioksidan kapasite ve enzim inhibisyonu aktiviteleri ve fitokimyasal kompozisyonunun ayrıntılı bir şekilde incelenmesi aşamalarından oluşmaktadır. Farmakobotanik çalışmalar P. anatolica bitkisinin diğer Plantago türlerinden köklerin inulin sferokristallerini içermesi, skapusun enine kesite 11 oluklu, yaprak sapının enine kesitte parankimatik hücreler içine gömülü 7 gelişmiş ve 2 tane az gelişmiş iletim demeti ile yaprağın alt ve üst yüzeylerinde anomositik ve polositik tipte stomaların olması ile anatomik olarak farklı olduğunu göstermiştir. Farmakognozik çalışmalar neticesinde P. anatolica bitki örneklerinden elde edilen ekstrelerin fenolik (kafeik asit, klorojenik asit, apicenin ve kemferol) ve lipofilik bileşikler (palmitik asit, linolenik asit ve benzotiazol) yönünden zengin kaynaklar olduğu tespit edilmiştir. Kafeik asit başta olmak üzere fenolik bileşikler yönünden zengin olarak tespit edilen ekstreler içerisinde, etanol fraksiyonu ve infüzyon ekstresi oldukça yüksek ORAC (1923 ve 1010 µmol Troloks eşdeğeri/g ekstre), FRAP (891 ve 660 µmol Fe2+/g ekstre), DPPH (IC50: 0,87 ve 0,34 mg/ml), demir şelatlama (IC50: 0,09 mg/ml), orta düzeyde antiamilaz (IC50: 4,31 ve 5,32 mg/ml), yüksek antiglukozidaz (IC50: 0,76 ve 0,64 mg/ml) ve antilipaz (IC50: 0,07 ve 0,04 mg/ml) aktiviteleri göstermiştir. Diğer Plantago türlerinden farmakobotanik açıdan farklı olan Plantago anatolica bitkisi fenolik ve lipofilik bileşikler açısından zengin bir kaynak olup, bu bitkiden elde edilen etanol fraksiyonu ve infüzyon ekstresi yüksek antioksidan ve enzim inhibitörlüğü özelliklerinden dolayı in vivo çalışmalar için seçilmiş aktif ekstreler olarak kullanılabilir.
Antioxidant Capacity and Phenolic Constituents of Malva Neglecta Wallr. and Plantago Lanceolata L. From Eastern Anatolia Region of Turkey
(Elsevier Gmbh, 2012) Dalar, Abdullah; Turker, Musa; Konczak, Izabela
Plantago lanceolata and Malva neglecta are two plants from eastern regions of Turkey used by the local population to cure multiple medical conditions, such as promoting maturation of abscesses, wound healing, abdominal pains and cancer. Both plants exhibited high oxygen radical absorbance capacity (ORAC assay) and total reducing capacity (ferric reducing antioxidant power, FRAP assay), comparable to or higher than herbs such as rosehip, cinnamon or oregano and multiple Chinese medicinal plants. Phenolic compounds were the major constituents of hydrophilic extracts and their levels correlated well with antioxidant activities. Leaf and flower/fruit, which are predominantly used as a medicine, contained the highest levels of phenolics, and comprised predominantly flavonoids and hydroxycinnamic acids. This may suggest that flavonoids and hydroxycinnamic acids play an essential role as physiologically active constituents of these traditional medicinal plants. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
Botanicals From Eastern Anatolia Region of Turkey: Antioxidant Capacity and Phenolic Constituents of Endemic Herbal Medicines
(Elsevier Gmbh, 2012) Dalar, Abdullah; Konczak, Izabela
Antioxidant capacity and polyphenolic composition of four endemic medicinal plants from the Eastern Anatolia Region of Turkey; Verbascum cheiranthifolium Boiss. var. cheiranthifolium Boiss., Dactylorhiza chuhensis Renz & Taub., Eryngium bornmuelleri Nabelek and Centaurea karduchorum Boiss. were evaluated for the first time. Aqueous plant extracts, representing decoctions traditionally used for medical purposes by the local population, exhibited high total reducing capacities (ferric reducing antioxidant power assay) and oxygen radical scavenging abilities (oxygen radical absorbance capacity assay) comparable to or higher than numerous Chinese and Ayurvedic medicinal plants and commonly used medicinal herbs. Flavonoids and hydroxycinnamic acids were two major phenolic groups present in the extracts. Extracts of leaves and flowers which are predominantly used for medical purposes exhibited the highest antioxidant capacities and polyphenolic content. Beside their application as traditional medicine, leaves and flowers might be utilized as sources of natural antioxidants. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
Centaurea Karduchorum Boiss. From Eastern Anatolia: Phenolic Composition, Antioxidant and Enzyme Inhibitory Activities
(Elsevier Gmbh, Urban & Fischer verlag, 2015) Dalar, Abdullah; Uzun, Yusuf; Mukemre, Muzaffer; Turker, Musa; Konczak, Izabela
The phenolic composition of lyophilized hydrophilic extracts obtained from root, stem, leaf and flower of Centaurea karduchorum Boiss. a medicinal plant endemic to Eastern Anatolia (Turkey) used as antidiabetic agent was characterized for the first time. Liquid chromatography mass spectrometry (LC/MS) analysis revealed that luteolin derivatives and chlorogenic acid were the dominating compounds contributing, respectively, from 16.1% (root) to 33.7% (stem) and from 7.3% (flower) to 12.0% (root) of total phenolics. Total reducing capacities of the extracts varied from 24.1 (root) to 38.5 (flower) mg gallic acid Eq./g DW [Folin-Ciocalteu assay] and from 274.0 (root) to 441.0 (stem) mu mol Fe2+/g DW [FRAP assay]. The oxygen radical absorbance capacity (ORAC assay) values varied from 930.5 (root) to 1853.5 (stem) mu mol trolox Eq./g DW. The leaf extract exhibited a pronounced inhibitory activity towards alpha-glucosidase (IC50: 0.63 +/- 0.00 mg/ml) and a weak inhibitory activity towards amylase (IC50: 14.63 +/- 0.67 mg/ml), which suggests a potential to reduce postprandial hyperglycaemia and supports its traditional use as antidiabetic agent. (C) 2015 Elsevier GmbH. All rights reserved.
Comprehensive Appraisement of Antioxidant Potential and Phytochemical Profile of Native Botanicals From Turkey
(Springer, 2019) Erez, Mehmet Emre; Dalar, Abdullah; Fidan, Mehmet; Pinar, Suleyman Mesut
This study presents the first comprehensive evaluation of antioxidant potential and phytochemical profiling of nine plant taxa utilized in southeastern and eastern parts of Turkey for various medicinal purposes, in order to contribute the scientific database, which was focused on exploring novel candidates of antioxidant agents from natural sources for industry and complementary medicine. Sequential extracts were prepared from plant materials were prepared through treating different solvents (acetone, ethanol, and water) based on polarity index. According to antioxidant assays via hydrogen atom transfer (HAT) and single atom transfer (SET) mechanisms, acetone extracts were found as the efficient antioxidant sources. Chromatographic studies revealed that phenolic compounds (mainly 5-O-Caffeoylquinic acid, luteolin hexoside, quercetin and rutin) were characterized as major hydrophilic phytochemical compounds and fatty acids (princibally linolenic, palmitic, and linoleic acids) were found as the major volatile compounds that dominantly contribute to the pronounced antioxidant capacities. According to the chemical structure, catechol moiety containing plant extracts had superior antioxidant capacity both for HAT and SET mechanisms. Data obtained from this study showed that among native botanicals, Crepis hakkarica, Crepis gemicii, Laserpitium carduchorum and Hedysarum vanaense might serve bioactive antioxidant agents both for industrial and pharmaceutical use.
Determination of Antioxidant Activities and Chemical Composition of Sequential Fractions of Five Edible Mushrooms From Turkey
(Springer india, 2020) Sezgin, Sema; Dalar, Abdullah; Uzun, Yusuf
Tricholoma scalpturatum, Tricholoma populinum, Neolentinus cyathiformis, Chlorophyllum agaricoides, and Lycoperdon utriforme have been traditionally utilized as food in Turkey for a long time. The present study focused on determining antioxidant activities, total phenolic groups contents (flavonols, hydroxycinnamic acids, proanthocyanidins, and anthocyanins), phenolic compounds and fatty acids of sequential extracts (n-hexane, ethyl acetate, chloroform, acetone, ethanol, and pure water) obtained from five wild edible macrofungi species. Ethanol and acetone (or ethyl acetate) were found as the most efficient solvents in terms of antioxidant activities and extraction efficiency. Antioxidant studies showed that L. utriforme, C. agaricoides, and T. populinum exhibited the highest radical scavenging and reducing activities and contained the highest phenolic contents. Chromatographic studies revealed that phenolic acids (protocatechuic, gallic, and chlorogenic acids) and fatty acids (oleic, linoleic, and palmitic acids) were the major contributors of the antioxidant activities of the extracts. The results obtained suggest the utilization of these macrofungi species as significant sources of natural antioxidants.
Determination of Biological Activity and Active Substances of Thecocarpus Carvifolius (Boiss.) Hedge & Lamond
(Springer, 2021) Pinar, Suleyman Mesut; Erez, Mehmet Emre; Fidan, Mehmet; Erodlu, Huseyin; Dalar, Abdullah
We have studied the antioxidant activity and analyzed active substances of the aqueous and methanol extracts of endemic species Thecocarpus carvifolius Hedge and Lamond. The antioxidant activity was identified and assessed using the DPPH, FRAP and iron chelating capacity assay. Active substances were detection, total phenolic, flavonoids and volatile compounds were analyzed. According to DPPH analyzes, the inhibition percentage was 48.57% for aqueous extract and 76.34% for methanol extract. The FRAP reduction capacity was determined as 3.32 mg/mL for aqueous extract and 11.75 mg/mL FESO4 for methanol extract. The total concentrations of phenolics and flavonoids were calculated as 64.08 - 35.29 mu g/mL and 146.84 - 555.66 mu g/mL in aqueous and methanol extracts, respectively. Finally, five volatile compounds were detected in methanol extracts. The results indicated that the endemic species of T. carvifolius can be evaluated as a natural antioxidant plant.
Doğu ve Güneydoğu Anadolu’da Yayılış Gösteren Bazı Endemik Türlerin Antioksidan Kapasitelerinin Belirlenmesi ve Etken Maddelerinin Tespit Edilmesi
(2016) Erez, Mehmet Emre; Dalar, Abdullah; Fidan, Mehmet; Pınar, Süleyman Mesut
Ülkemiz, cografik konum ve jeolojik yapı bakımından, farklı topografik yapılara ve toprak gruplarına sahip, degisik iklim tiplerinin etkisi altında ve üç farklı bitki cografyası bölgesinin birlestigi yerde bulunmasınedeni ile zengin bir flora ile çok degisik vejetasyon tiplerine sahiptir. Ayrıca ekolojik ve floristik özelliklerine de baglı olarak,bazı bitki cinslerinin gen merkezi konumundadır. Bütün bu özellikler Türkiye Florası?nın ilginçligini ve çesitliligini açıkça ortaya koymaktadır. Bu amaçla özellikle Dogu ve Güneydogu Anadolu?da endemik tür oldukları bilinen; ? Crepis hakkarica Lamond, ? Laserpitium carduchorum Hedge, Lamond, ? Crepis gemicii Yıldırım, Bingöl ,Armagan, ? Jurinea cataonica Boiss. , Hausskn. ex Boiss., ? Hedysarum vanense Hedge , Hub.-Mor., ? Trigonella macrorrhyncha Boiss., ? Anacyclus anatolicus Behçet , Almanar, ? Dianthus vanensis Behçet , Ilçim, ? Astragalus güzelsuensis F. Ghahrem. Behçet, Demir bitki türlerinin antioksidan kapasiteleri ve etken madde içerikleri tespit edilmeye çalısıldı. Bu baglamda bitki örneklerinin analizi için farklı ekstraksiyon yöntemleri uygulandı. Elde edilen veriler degerlendirildiginde bitkilerin yüksek fenolik ve flavonoid bilesiklerini içerdikleri, ayrıca yüksek DPPH ve FRAP aktivitelerine sahip olduklarıtespit edildi. Ayrıca, bitkilerin habitatlarına baglı olarak enzimatik antioksidan seviyelerinin de yüksek oldugu belirlendi. Bitkiler familya ve taksonlarına baglı olarak, uçucu yag ve etken madde bilesikleri açısından farklı kalitatif ve kantitatif degerlere sahip oldukları tespit edildi. Proje; bölgede bulunan güvenlik sorunlarına ragmen bir yıl gibi kıssa bir süredeön görülen zamanda tamamlandı. Elde edilen veriler degerlendirildiginde, tüm bitkilerin farklı farmokolojik özelliklere sahip olduklarıancak özellikle Crepis hakkarica bitkisinin çalısılan diger bitki türlerindendaha iyi sonuçlar gösterdigi tespit edildi. Bu bitkiden elde edilen ektraktlar içiniçin yeni proje ve biyo-uygulamaların yapılması gerektigi ileri sürüldü. Sonuç olarak çalısılan bitkilerden elde edilen veriler ısıgında yayınlanacak çalısmaların, ülke ve bölge degerlerinin anlasılmasınayardımcı olacagı düsünülmektedir.
The Effects of Abiotic Stressors and Signal Molecules on Phenolic Composition and Antioxidant Activities of in Vitro Regenerated Hypericum Perforatum (St. John's Wort)
(Elsevier, 2020) Eray, Nese; Dalar, Abdullah; Turker, Musa
Hypericum perforatum L. is a pharmaceutically and industrially significant natural source due to its rich phenolic content and antioxidant potential. This study aimed to establish an optimized development protocol through plant tissue culture methods and evaluate the effects of abiotic stressors and signal molecules on phenolic composition and antioxidant responses. Methanol based extracts prepared from lyophilized powders of plant samples obtained from nature and aseptic seedlings, and calluses obtained from abiotic stress factors and signal molecules were analysed for phenolic composition using HPLC-MS/MS and antioxidant responses through complementary methods (FCR, FRAP and ORAC). Experimental analysis revealed the use of B5 and SH media for in vitro plant regeneration as the most proper chemical environments for the development and standardization of Hypericum perforatum. Chromatographic findings showed that quercetin, pseudohypericin and partially chlorogenic acid compounds as key phenolic compounds of calluses and plant samples of Hypericum perforatum. The analyses deduced that UV and sucrose increased the level of chlorogenic acid, while ascorbic acid increased the level of quercetin and drought applications increased the concentration of pseudohypericin compared to the control. Rutin compound was only produced in pot samples, which indicates a significant response to the drought stress. Additionally, it was found that B5, SH, and MS media containing of 2 mg/L NAA+1 mg BA with elicitors of 50 mu mol of ascorbic acid and 6% sucrose and a UV stress of 5 (or 10) min-24 h harvesting might be a proper protocol for the optimization and standardization of Hypericum perforatum L. Findings obtained within this study revealed novel contributions for optimization, standardization and production of antioxidative phenolic compounds of Hypericum perforatum plant samples for pharmaceutical and industrial utilization. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
Electrochemical Biosensing: From Interaction Between Epilobium Species and Dna To an Approach To Detect New Electroactive Components in Plant Extracts and Their Effects on Dna
(Springer Heidelberg, 2024) Subak, Hasret; Dalar, Abdullah; Mukemre, Muzaffer; Ozkan-Ariksoysal, Dilsat
Here, a simple and label-free biosensor based on disposable pencil graphite electrode (PGE) was developed for the first time to investigate the interaction between Epilobium sp. plant extract containing active compounds (quercetin and kaempferol) and DNA using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The solution of extract was interacted with calf thymus double-stranded DNA (dsDNA) which immobilized onto the biosensor surface by simple adsorption. The effect of Epilobium on double-stranded DNA (dsDNA) was then analyzed by monitoring the changes in electrochemical signals arising from both guanine bases in DNA and the electroactive compounds in Epilobium extract. Epilobium content was also detected by using HPLC/MS/MS technique, and the gained results were compared with those obtained by electrochemical method. It was found that both methodologies supported each other in terms of active ingredients in Epilobium sp. Numerous factors affecting the extract-DNA interaction were optimized such as Epilobium concentration, interaction time. The developed DNA sensor can well detect Epilobium extract-DNA interaction in 60-min detection time with 26 ng of detection limit in 50 mu L of sample volume with a linear range from 1 to 10 mu g/mL. This study includes alternative method that can be used to detect new electroactive components present or to be found in different plant extracts and their interactions with DNA.
Electrochemical Detection of Interaction Between Verbascum Sp and Dna by Using Disposable Biosensors
(Elsevier Science Bv, 2017) Subak, H.; Dalar, Abdullah; Ozkan-Ariksoysal, D.
Verbascum sp. known as mullein, comprise approximately 250 species of flowering plants, native to Europe and Asia. Multiple species of mullein are commonly used as a plant based nutritional supplement and herbal tea. Infusion and/or decoction prepared from Verbascum cheiranthifolium Boiss. var. cheiranthifolium Boiss. (Scrophulariaceae), known as masicerk, is extensively used to cure rheumatism, menstrual pains and haemorrhoids in Eastern Anatolia. There has still been groving interest in studying DNA-targetted bio(nano) sensors and DNA interactions in recent years. In this study, the electrochemical behavior of the plant extract was monitored and the effect of this extract on DNA was investigated by using biosensor technology. The interaction mechanism of the extract was also examined and evaluated with disposable carbon electrodes by using differential pulse voltammetry (DPV). Thus, the optimal experimental parameters were determined and compared with GC-MS data. The obtained results showed that newly-developed biosensor could be used as a model device for the rapid, cost effective and sensitive detection of plant extract-DNA interaction.
Evaluation of the in Vitro and in Vivo Effects of the Ethanolic Lyophilized Extract Obtained From Pholiota Aurivella Mushroom on Cci4-Induced Toxicity in Rats
(Parlar Scientific Publications (p S P), 2019) Dogan, Abdulahad; Dalar, Abdullah; Battal, Abdulhamit; Uyar, Ahmet; Sadullahoglu, Cemil; Celik, Ismail; Uzun, Yusuf
The aim of this study was to evaluate Pholiota aurivella extract's chemical and mineral composition, total antioxidant capacity, histopathological changes in liver and kidney, as well as its possible protective/harmful role against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, twenty four rats were divided into four experimental groups: Control, CCl4, CCl4+P. aurivella (100 mg/kg, extract) and CCl4+P.aurivella (500 mg/kg, extract) groups. Some biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) in serum, malondialdehyde (MDA) and antioxidant defense system (ADS) constituents such as reduced glutathione (GSH) levels, glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities in the liver, brain and kidney tissues, histopathological changes in liver and kidney of rats were observed. P.aurivella extract was rich in phenolic (p-coumaric and protocatechuic acids) and fatty acid (linoleic, oleic and palmitic acids) compounds and high level of Arsenic. In vivo studies revealed that P.aurivella extract suppressed the levels of ADS enzymes and increased the MDA content and AST, ALT and LDH in treated groups. According to histopathological investigation, liver and kidney revealed severe histopathological changes both in CCl4 group and P.aurivella- treated groups. These results have shown that oral administration of P. aurivella extract caused a significant toxic effects in rats. Therefore, in the context of the data obtained from this study the P. aurivella could not be regarded as a safe food source.
Health Attributes of an Endemic Orchid From Eastern Anatolia, Dactylorhiza Chuhensis Renz&taub. - in Vitro Investigations
(Elsevier Gmbh, 2015) Dalar, Abdullah; Guo, Yu; Esim, Nevzat; Bengu, Aydin Sukru; Konczak, Izabela
Phytochemical composition and potential health attributes of Dactylorhiza chuhensis Renz&Taub., an endemic orchid from Eastern Anatolia, were investigated. Lyophilized methanol-based extracts obtained from leaf, flower, stem and tuber were investigated for the presence of phenolic compounds [Folin-Ciocalteu assay and high performance liquid chromatography analysis (HPLC)], antioxidant capacities [ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assay] and enzyme-inhibitory activities [lipase, alpha-amylase, alpha-glucosidase and angiotensin converting enzyme (ACE)]. The tuber, used as a traditional remedy and utilized by the pharmaceutical industry, had the lowest content of total phenolics, inferior antioxidant and enzyme-inhibitory activities. The highest phenolic content (44.9 +/- 0.8 mg GAE/g DW) was exhibited by the leaf extract, which also showed superior reducing (736.8 +/- 16.2 mu mol Fe2+/g DW) and oxygen radical scavenging capacities (2715.8 +/- 83.5 mu mol Trolox E/g DW). The inhibitory activities of the leaf extract toward alpha-amylase, alpha-glucosidase and ACE were moderate. Applied at non-toxic concentrations, the leaf extract effectively reduced accumulation of nitric oxide (NO) in lipopolysaccharide (LPS)-activated hepatocellular carcinoma (HepG2) cells. Further studies towards potential utilization of D. chuhensis leaf as a source of physiologically active phytochemcials are justified. Crown Copyright (C) 2015 Published by Elsevier GmbH. All rights reserved.
Identification of Phenolic Compounds and Evaluation of Potential Health Benefits of Eryngium Bornmuelleri Nab.
(2013) Dalar, Abdullah; Türker, Musa
Bu çalışmada ülkemizin Doğu Anadolu Bölgesinde yerel halk tarafından, ağrı ve kanser tedavisinde kullanılan Eryngium bornmuelleri Nab. bitkisine ait yaprak organından elde edilen ardışık fraksiyonların fenolik kompoziyonunun tanımlanması ve potansiyel sağlık değerlerinin belirlenmesi ile ilgili çalışmalar yürütülmüştür. Sıvı metanol ekstreleri üzerine yürütülen ön çalışmalar sonucunda, E. bornmuelleri bitkisine ait yaprak organının, incelenen örnekler içerisinde en yüksek oksijen radikal absorbans kapasitesi (1489±17 µmol Troloks Eş./g KA), ferrik indirgeme gücü (250.3±2.2 µmol Fe2+/g KA ), Folin-Ciocalteu indirgeme aktivitesi (25.8±0.2 mg Gallik asit Eş./g KA) gösterdiği ve yüksek miktarda flavonoid (6.89±0.72 mg Rutin Eş./g KA), flavonol (5.27±0.20 mg Rutin Eş./g KA), hidroksisinnamik asit (2.89±0.02 mg Kafeik asit/g KA) ve düşük miktarda proantosiyanidin (0.012±0.001 mg Katekin Eş./g KA) bileşiklerini içerdiği tespit edilmiştir. Daha sonra yürütülen çalışmalar sadece E. bornmuelleri?nin yaprak örnekleri ile devam edilmiştir. Yaprak örnekleri sırasıyla etanol, aseton ve saf su çözücüleri ile ardışık olarak fraksiyonlara ayrılmıştır. Sıvı kromatografisi ve kütle spektrumu analizleri sonucunda hidrofilik ardışık fraksiyonların, kuersetin rutinosid (rutin), çeşitli flavonoid glikozitleri (kamferol glukozit, kamferol rutinosid ve kuersetin glukozit), klorojenik asit, kaftarik asit ve hidroksibenzoik asit hekzositten oluşan zengin fenolik bileşikler kaynağı olduğu tespit edilmiştir. Korelasyon analizleri (R2 ?0.7-0.9), fenolik bileşiklerin E. bornmuelleri bitkisinin yaprak kısmından elde edilen ardışık fraksiyonlardaki biyoaktivitelerden sorumlu bileşikler olduklarına işaret etmektedir. Kuersetin rutinosid (Rutin) yönünden zengin olarak tespit edilen fraksiyonlar içerisinde, etanol fraksiyonu en yüksek fenolik içerik (173.7±3.1 mg Rutin Eş./g KA) ve biyoaktiviteye sahip olup, bu fraksiyonda oldukça yüksek ORAC (4415±177.1 µmol Troloks Eş./g KA), FRAP (890.2±41.6 µmol Fe2+/g KA) ve FCR (809±1.6 mg Gallik asit Eş./g KA), orta düzeyde antiamilaz (IC50:8.3±0.1 mg/ml) ve antiglukozidaz (IC50:8.47±0.14 mg/ml), yüksek antilipaz (IC50:5.92±0.10 mg/ml), antihipertansiyon (IC50:2.6±0.1 mg/ml), hücresel antioksidan (EC50:1.048±0.02 mg/ml), H2O2 kaynaklı hücre ölümüne karşı koruyucu aktivite (0.8 mg/ml uygulamasında, %92.58 hayatta kalma oranı), antienflamatuar ve antiproliferativ (IC50:5.27±0.04 mg/ml) değerleri belirlenmiştir. Sonuç olarak, fenolik bileşikler açısından zengin bir kaynak olan Eryngium bornmuelleri Nab. bitkisine ait yaprak organı antioksidan, antihipergilisemik, antihiperlipidemik, antihipertansiyon, hücresel kemokoruyucu, antienflamatuar ve antikanser aktivite gösterdiğinden dolayı, alternatif veya tamamlayıcı bir polifarmakolojik ajan olarak kullanılabilir.
In Vitro Antioxidant and Enzyme Inhibitory Properties and Phenolic Composition of M. Neglecta Wallr. (Malvaceae) Fruit: a Traditional Medicinal Fruit From Eastern Anatolia
(Elsevier, 2013) Turker, Musa; Dalar, Abdullah
Malva neglecta Wallr. (Malvaceae) fruit is an edible traditional medicinal plant used in Eastern Anatolia to cure multiple medical conditions such as asthma, stomach ache, diarrhoea, etc. In this study, lyophilized ethanol-based hydrophilic extract was investigated for total phenolic content and phenolic composition using liquid chromatography coupled with mass spectrometry, in vitro antioxidant capacity (FCR, FRAP and ORAC assays) and enzyme inhibitory activities. Ethanol-based hydrophilic extract prepared from M. neglecta fruit was identified as a rich source of phenolic compounds, exhibited pronounced Folin-Ciocalteu reducing activity (12.8 +/- 0.7 mg gallic acid Eq./g DW), ferric reducing antioxidant power (174.3 +/- 9.8 mu mol Fe2+/g DW) and oxygen radical scavenging activity (1656.9 +/- 69.7 mu mol Trolox Eq./g DW) and weakly suppressed the activity of alpha-amylase (IC50: 15.2 +/- 0.8 mg/ml), a-glucosidase (IC50: 14.19 +/- 0.10 mg/ml) and pancreatic lipase (IC50: 17.55 +/- 0.20 mg/ml). Flavonoid glycosides were tentatively detected as the major phenolic compounds. Furthermore, traces of rutin, chlorogenic acid, hydroxybenzoic acid and hydroxybenzoic acid-O-hexoside were also detected in the extract. High levels of antioxidant capacities and complex mixture of phenolic compounds of M. neglecta fruit revealed that this fruit might be a good source for natural antioxidants. (C) 2013 Elsevier B.V. All rights reserved.
In Vitro Propagation and Secondary Metabolites Investigation of Hypericum Perforatum L
(Parlar Scientific Publications (p S P), 2019) Mohammed, Namam Rebaz; Dalar, Abdullah; Ozdemir, Fethi Ahmet; Turker, Musa
Hypericum perforatum L. was regenerated in plant tissue culture and secondary metabolites (hypericin, pseudohypericin, quercetin, rutin, and chlorogenic acid) of the plants collected from field and regenerated in vitro were quantitatively compared. The liquid, semi solid and solid form of Murashige and Skoog (MS) basal medium supplemented with plant growth regulators (PGRs) in different concentration and combination were employed for regeneration and secondary metabolite product amplification. Based on preliminary tests nodal segment was preferred as an explant. At the end of 50 days of regeneration period, no statistically significant difference was observed between the length of root and shoot and root number of plants regenerated in solid, semi solid and liquid media supplemented with different PGRs. The quantitative secondary metabolite analyses was performed by High Performance Liquid Chromatography (HPLC). The highest concentrations of chlorogenic acid, quercetin, and pseudohypericin were observed in shoots and roots of the plants collected from field. Whereas the compounds were detected in low quantity in plants regenerated in the liquid, semi solid and solid medium, except for quercetin which was found higher concentration than chlorogenic acid, quercetin, and pseudohypericin in vitro regenerated plants. Hypericin and rutin were not detected by HPLC analysis in all of plants regenerated in vitro and in vivo.
The Investigation of The Medicinal Potential of Alcea Kurdica Alef. in Nature and Tissue Culture
(Soc Stiinte Farmaceutice Romania, 2022) Gormez, Gul; Battal, Abdulhamit; Dalar, Abdullah; Turker, Musa
Alcea kurdica Alef. (AK) a hollyhock has been used in folk medicine to treat various diseases. Though its extensive utilization in traditional medicine, experimental studies regarding to its phytochemical composition is limited. The aim of this study was to determine the antioxidant capacity and phenolic composition of ethanol extract of AK plant collected from the natural environment in different vegetation periods (June-flowering, July-fruiting, August-seeding) and to control its antioxidant capacity and phenolic composition by in vitro shoot tip culture. AK plant at the flowering period had the highest antioxidant capacity, while the lowest activity was determined at the seeding period according to results. Phenolic acids dominated by gallic, chlorogenic and caffeic acids were determined as the major phenolics. According to in vitro results, the use of 1-naphthylacetic acid (NAA) and 6-benzylaminopurine (BA) alone caused significant decrease in the three phenolic acids content compared to the control. On the other hand, combination of NAA and BA caused significant increase in phenolic acids content compared to the control. Moreover, significant increase in ORAC was observed in AK extract obtained from in vitro Murashige & Skoog (MS) media culture supplemented with NAA and BA combination. 15 min of UV exposure negatively affected the antioxidant capacity and phenolic content.