Browsing by Author "Dede, S."

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Age and Serum Mineral Profile in Turkey
(indian veterinary Journal, 2007) Dede, S.; Yur, F.; Yoruk, I.
The objective of this research was to determine the effect of age on the concentration of minerals in the turkey.
Antioxidant Vitamins and Microminerals in Cows With Foot-And Disease
(Agricultural Research Communication Centre, 2014) Yoruk, I. H.; Tanritanir, P.; Dede, S.; Ceylan, E.; Ragbetli, C.
Foot and-mouth disease (FMD) is an acute, highly contagious inflammatory viral disease of domestic and wild animals which causes decrease in productivity and lead to mortality. This study was conducted to investigate the levels of copper, zinc, magnesium and iron, alpha-tocopherol, vitamin D-3, vitamin C and lipid peroxidation in cow with foot-and-mouth disease. Sixty native black cows infected by foot-and-mouth disease and twenty healthy controls were selected for the study after clinical and serological examination. In the diseased animals, the malondialdehyde (p<0.00) and-copper (p<0.001) levels were increased while the vitamin C, alpha-tocopherol (p<0.001), vitamin D3 (p<0.05) magnesium (p<0.001) and iron (p<0.005) levels were decreased relative to controls. Although the zinc level was not changed, the Cu/Zn ratio was elevated in the diseased group. The obsereved changes may be the result of secondary infections due to suppressed immune system and losses of tissue and mucosa associated with foot-and-mouth disease.
Effect of Substituting Barley Grain With Wet Sugar Beet Pulp Silage on Some Blood Metabolites in Lambs
(Agricultural Research Communication Centre, 2019) Aldemiri, R.; Bingol, N. T.; Karsli, M. A.; Dede, S.
The objective of this experiment was to evaluate the effects of substituting grain barley with we sugar beet pulp (WSBP) silage prepared with wheat bran (WSBPS) at different levels on some blood parameters of lambs. A barley grain based control diet was prepared. Then, three experimental diets were prepared by substituting 35, 70 and 100% of barley grain with WSBPS (35, 70 and 100% WSBPS diets). Moreover, negative control was also established. A total of sixty, 6-7 month old male Akkaraman lambs with 24.29+1.50 kg mean body weight were used in the study. These lambs were fed one of five diets for a period of 75 days. Blood samples were taken at the beginning and end of the experiment. Serum glucose, total protein, BUN, phosphorus, and calcium levels were determined. Serum BUN and phosphorus levels were similar at the beginning and end of the experiment (P>0,05). Serum glucose levels were significantly higher in control, 100% WSBPS and negative control whereas Ca level was higher in all groups, except negative control at the end of experiment (P<0,05). Total protein levels were significantly lower in 70 and 100% WSBPS (P<0,05). All of the parameters, except BUN level were statistically similar among groups at the end of the experiment. In conclusion, substituting barley with wet sugar beet pulp silage did not have significant effects on blood parameters, except BUN. Substituting barley with WSBPS could be an alternative feeding strategy for farmers without affecting animal health based on the results of this experiment.
The Effect of Thymoquinone Treatment on Total Oxidant and Total Antioxidant Level in Experimental Diabetic Rats
(Ataturk Universitesi, 2018) Usta, A.; Dede, S.; Çetin, S.
In this study, the effects of thymoquinone implementation to total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were investigated in liver and kidney tissues of diabetic rats induced with STZ. The 24 male Wistar-Albino rats, weighing between 200-250 g were used as material. Rats were divided into four groups, each consisting of six rats; control (C), thymoquinone (TQ), diabetes (D) and diabetes+ thymoquinone (DT) groups. To induce diabetes, a single dose of 45mg/kg streptozotocin (STZ) were administered intraperitoneally (ip) to the rats in groups D and DT. TQ was administered to the rats in DT and TQ groups as 30 mg/kg/21 days by oral gavage. The values of TAS and TOS were measured in liver and kidney tissues at the end of the study. OSI was calculated according to TAS and TOS levels. TAS value in liver tissue was found to be the lowest in the diabetes group (P≤0.05). It was noticed that there was no difference between K and TQ groups with DT group. No difference was detected among groups in terms of liver TOS. It was determined that the OSI in the liver increased in all groups compared to the control (P≤0.05), the highest value was determined in group D, it approached to the control by partially decrease after the application of TQ. While TAS and TOS levels in kidney tissue were found to be the highest in the TQ group and the lowest in the diabetic group (P≤0.05), there was no difference between the other groups. There was no significant difference among the groups in the kidney OSI. As a result, it was determined that TOS, TAS values in liver and kidney tissues of experimental diabetic rats were affected from the TQ application and the OSI value in the liver of the STZ treated groups approached that of the control group, after TQ application. © 2016 Ataturk Universitesi. All rights reserved.
The Effect of Vitamin C and Vitamin E Administration for Treatment and Protection on Serum Vitamin Levels in Rats With Experimentally Fluorosis Created
(Ataturk Universitesi, 2021) Öner, A.C.; Çetin, S.; Yörük, I.H.; Dede, S.
This study aimed to determine the vitamins A-D and E levels in rats with experimental fluorosis and in whom vitamins, C and E were administered for prevention and treatment. One-month-old Wistar-Albino rats were used as material in the study. Nine groups of 8 animals each were studied. Sodium fluoride (NaF) solutions (150 mg/kg) prepared in drinking water were administered ad-libitum during the study. Vitamin C (100 mg/kg), Vitamin E (300 mg/kg) and Vitamin C + Vitamin E (100 mg/kg + 300 mg/kg) were administered to the protection group with water containing 150 ppm NaF for 16 weeks/day. Water containing 150 ppm NaF was given ad-libitum for 16 weeks in the treatment group, and then Vitamin C (100 mg/kg), Vitamin E (300 mg/kg) and Vitamin C + Vitamin E (100 mg/kg + 300 mg/kg) were administered along with normal drinking water for 4 weeks. At the end of sixteen weeks, blood samples were taken from the rats and their serums were removed. Serum Retinol, α-Tocopherol, Vitamin D3 levels were determined by HPLC. In the fluorosis group, it was found that there was no change in serum vitamin A and vitamin D levels with the application of vitamins C and E for treatment and protection, but a decrease in vitamin E level. As a result, it was concluded that the administration of vitamin E is effective in both the prevention and treatment of fluorosis cases. © 2021 Ataturk Universitesi. All rights reserved.
Effects of Green Tea on Mineral Levels of Liver and Testis of Guinea Pigs Electromagnetic Field Emitted by Mobil Phones
(2009) Kiliçalp, D.; Dede, S.; Deger, Y.; Aslan, L.
It was reported that the effects of green tea on the mineral levels of testis and liver of Guinea pigs exposed to a 900 MHz electromagnetic field. Four experimental groups labeled as controls (Group A), irradiated (Group B), irradiated receiving green tea extract (Group C) and green tea only (Group D) were formed with seven randomly chosen animals of both sexes in each group. After exposure for one month, the animals were sacrificed by decapitation and testis and liver samples were collected for biochemical analysis. In female Guinea pigs irradiation with and without green tea as well as green tea alone caused significant changes of the iron levels in liver, but no significant changes of manganese, copper, zinc and the copper/zinc ratio. In males, irradiation caused significant increases of manganese and a decrease of the iron levels in liver and of manganese, copper, zinc in testis. Combined with green tea, electromagnetic radiation resulted in changes of manganese, iron, copper and copper/zinc ratio in liver and of manganese only in testis. Green tea alone changed the levels of hepatic iron, zinc and copper/zinc ratio and of testicular concentrations of iron and zinc. The highest levels of copper were found in the liver tissue of the irradiated animals that were also treated with green tea. From present findings we can state that testis tissue is more sensitive to electromagnetic radiation than liver tissue, showing greater changes in trace mineral metabolism. Green tea brings the trace element levels to near normal values; supporting the idea that green tea as a supplement has a protective effect against the damaging effects of electromagnetic radiation. © 2009 Academic Journals Inc.
Effects of Plantago Major Extract on Serum Levels of Antioxidant Vitamins and Minerals in Broiler
(Agricultural Research Communication Centre, 2018) Dede, S.; Bingol, N. T.; Kilinc, D. Kilicalp; Deger, Y.; Yoruk, I. H.; Ekici, P. Tanritanir; Karsli, M. A.
The aim of this study was to evaluate the effects of Plantago major liquid extract on serum antioxidant vitamin and mineral concentrations of broiler. The experiment consisted of the control and 2 treatment groups, composed of 28 Ross 308 broiler chicks (Total, 84). A basal (control) diet was formulated. P Major was added to the control diet as following: PM1 (5 g/kg feed), PM2 (10 g/kg feed). The chicks were feed for 42 days ad libitum. The serum micronutrients (Retinol, alpha-tocopherol, vitamin D, calcium, magnesium, iron, manganese, and zinc) levels were determined. The retinol, alpha-tocopherol, Fe, Mg, Mn levels were not affected, and the Zn levels decreased in the PM1 and PM2. The supplementation of P. major has affected the serum vitamin D and Zn levels for this study, and should be analyzed for the alterations on the metabolism in subsequent studies.
Effects of the Replacement of Soybean Meal With Pea as Dietary Protein Source on the Serum Protein Fractions of Broilers
(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2016) Bingol, N. T.; Dede, S.; Karsli, M. A.; Deger, Y.; Kilinc, Kilicalp D.; Cetin, S.
The aim of this study was to determine the effects of the replacement of different levels of protein derived from soybean meal with that from peas in broiler diets on serum protein fractions. A corn-soybean meal basal diet was formulated as the control diet (Control=C) (NRC, 1994), and then pea was added to the control diet to replace 20% (P20) or 40% (P40) of the crude protein of the control diet. The diets were randomly fed to 12 pens per treatment, each housing five birds, for 42 days. Blood samples were collected from 36 birds (3 birds x 4 pens x3 treatments) and the serum protein fractions were separated. Gamma-globulin percentage was higher in group P20 compared with C and P40 groups. Total protein, beta-globulin, and gamma-globulin concentrations were significantly higher in group P20 compared with those of both control and P40 group (p<0.05).
The Effects of Vitamin D Onto the Expression of Caspase Enzymes in Osteoblastic Cell Line Treated With Sodium Fluoride (Naf)
(Wiley, 2017) Yuksek, V.; Dede, S.; Taspinar, M.
Electrophoretic Profile of Serum Protein Fractions From Sheep Naturally Infected With Babesia Ovis
(Ecole Nationale veterinaire Toulouse, 2010) Apaydin, B.; Dede, S.
The aim of this study was to investigate the alterations of the serum protein electrophoretic profile in sheep babesiosis. For that, blood sera were sampled from 20 clinically healthy without protozoon Akkaraman sheep, 1.5 to 2.0 years old, stemming from the Van region (Turkey) in one hand and from 36 Akkaraman sheep, naturally infected with B. ovis and with clinical signs before and 5 days after anti-parasitic treatment in the other hand and were analyzed by cellulose acetate electrophoresis. The total proteinemia (P < 0.01) as well as the concentrations of all serum protein fractions (albumin, alpha(1)-, beta-, gamma-globulins and particularly alpha(2)-globulins, P < 0.05 to P < 0.001) were dramatically decreased in diseased animals before treatment compared to controls. By contrast, the total protein concentrations and the absolute globulin concentrations significantly increased 5 days after treatment (except for the alpha(1)-globulin concentrations) but they remained weakly below the control values. Furthermore, the ratio A/G significantly increased in parallel. These results suggest that B. ovis infection has induced an intense proteolysis of the circulating proteins probably coupled to withdraw of liver protein synthesis and dehydratation which was alleviated since parasite eradication.
Expression Levels of Some Apoptotic and Oxidative Genes in Sheep With Sarcocystosis
(Hellenic veterinary Medical Soc, 2022) Yuksek, V.; Kilinc, O. O.; Dede, S.; Cetin, S.; Ayan, A.
Sarcocystosis is a zoonotic protozoon-related disease with a very broad intermediate host spectrum. These protozoon parasites lead to tissue loss in their intermediate hosts. The purpose of this study was to present the mRNA expression levels of some genes belonging to the oxidative stress and apoptosis pathway systems in tissue damage caused by sarcocystosis. In this study, the material consisted of infected tissue taken from sheep esophagus determined to be sarcocystosis-infected and esophageal tissues taken from healthy sheep. The expression levels of the GPX1, SOD1, SOD2, NCF1and Nos2 genes that play a role in the oxidative stress mechanism and the caspase 3, 8, 9 and BCL-2 genes that play a role in the apoptosis mechanism were determined by RT-qPCR. As a result of the study, it was determined that, with increased oxidative stress, the gene expressions related to the relevant enzyme systems also increased, and in relation to this increase, the caspase enzyme genes that are effective in cell death were up-regulated. These results may shed light on similar studies for understanding and preventing damage mechanisms that may form as a result of sarcocystosis. As a result, it is understood that increased oxidative stress parameters and increased apoptosis in sarcocystic tissue in sheep cause tissue loss. We think that understanding the molecular mechanisms of this disease is clinically important in the treatment of parasitic diseases and in the prevention of economic losses that may occur as a result of the disease.
Genetic Variability of Alpha-Casein, Beta-Casein, and Kappa-Casein Genes in Holstein-Friesian, Simmental and Brown Swiss Cattle
(Hellenic veterinary Medical Soc, 2025) Cak, B.; Dede, S.; Yuksek, V; Yilmaz, O.; Demirel, Af
This study aims to determine the genotypes and allele frequencies of polymorphisms of bovine alpha S1-ca-sein (CSN1S1), alpha S2-casein (CSN1S2), /3-casein (CSN2) and kappa-casein (CSN3) genes in Holstein-Friesian, Simmental, and Brown Swiss breeds. DNA was isolated from milk samples and gene regions were amplified using optimized PCR protocols. Sanger sequencing was performed to genotyping PCR products. CSN1S1 and CSN1S2 genes in three cattle breeds were observed to be monomorphic. Three different genotypes were observed for the CSN2 gene in Holstein-Friesian and two genotypes in Brown Swiss, while the Simmental breed was monomorphic. For the CSN3 gene was observed three different genotypes in Holstein-Friesian and Brown Swiss, two different genotypes in Simmental. Also, a novel single nucleotide polymorphism (SNP) was identified in the sequence region in the CSN2 gene exon 7 of three cattle breeds in this study. This SNP, c.249C>G, were non-synonymous, which leads to the change of p.N68K in amino acid residue of bovine /3-casein. This SNP is thought to be detected for the first time in Bos taurus genus. However, it was concluded that it would be appropriate to carry out more studies on this new SNP point in larger populations.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride
(Humana Press inc, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Prolonged exposure to high doses of fluoride causes chronic poisoning called fluorosis, which affects many tissues and causes serious health problems. This study was planned to investigate the apoptotic, autophagic, and necrotic molecular pathways of fluoride. Sodium fluoride (NaF) was administered to normal rat kidney epithelial (NRK-52E) cells. The NaF IC50 value was determined using the MTT assay. The expression of the genes in the autophagic, apoptotic, and necrotic pathways was determined by real-time PCR. It was determined that there were significant changes in NaF-induced molecular pathways depending on the time. There were no increases in apoptotic and necrotic pathway markers except for Atg3, an autophagy gene, at the 3rd and the 12th hours. However, there was an induction in all cell death signaling pathways at 24 h. The molecular mechanisms demonstrated NaF-induced cellular death in the NRK-52E cell line. It was concluded that these molecular mechanisms were activated with NaF, and different mechanisms accelerated the cellular death at the 24th hour.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride (Nov, 2020, 10.1007/S12011-020-02491-3)
(Springernature, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Investigation of the Effects of Carrying Heavy Load on Prooxidation/Antioxidant Status and Vitamin D3 in Healthy Horses
(Academic Journals inc, 2009) Ceylan, E.; Dede, S.; Deger, Y.; Yoeruek, I.
The aim of the study was to investigate the effect of carrying heavy load for a long time on lipid peroxidation (MDA: malondialdehyde), NO2 (nitrite), NO3 (nitrate), antioxidants (GSH: reduced glutathione, retinol, alpha-tocopherol) and vitamin D-3 in healthy horses. Blood samples from seventeen native 3-5 years age and 450-500 kg live weight Anatolian horses carried a load which comprised at least 30% of their body weight and for 4 h on mountainous terrain (hard working) were evaluated. Blood samples were collected in the morning before the animals started to carrying load and immediately after they finished carrying (working). It is observed that the level of MDA, NO2 and NO3 increased significantly (p<0.05) after working. While GSH concentration, increased after working; levels of retinol, alpha-tocopherol and vitamin D-3 levels decreased significantly (p<0.05). On the other hand, the vitamin D-3 levels were affected by hard working as other lipid soluble vitamins. There were a correlation between the physiological response to hard-working and some oxidant markers in healthy-hard working horses. These observations provide evidence that hard-working increases oxygen consumption and cause a disturbance of intracellular prooxidant-antioxidant homeostasis.
The Investigations of Total Antioxidant Status and Biochemical Serum Profile in Thymoquinone-Treated Rats
(African Networks Ethnomedicines, 2015) Kurt, E.; Dede, S.; Ragbetli, C.
Background: This study was planned to determine the dosage and duration of thymoquinone (TQ) at which it demonstrates the optimum effect on the total antioxidant status (TAS) and the biochemical parameters in the blood serum. Materials and Methods: 48 male rats (Wistar-albino) weighing between 200-250 g were used as material. Group 1 (control) (TQ solution 5 mg/kg/day), Group 2 (15 mg/kg/day), Group 3 (30 mg/kg/day), Group 4 (45 mg/kg/day) and Group 5 (60 mg/kg/day) were designated, each containing 8 rats. Different doses of TQ (oral gavage) were administered for four weeks. Results: The TAS levels were determined to be considerably low statistically in all TQ-administered groups in comparison with the control group. It was determined that the serum biochemical parameters exerted a significant effect depending on the TQ doses. Conclusion: As a result, rats administered with TQ orally, at 60 mg/kg dosage, show that the liver and kidney function parameters in particular as different from normal. This brings us to the conclusion that at this dosage, there is reliable biochemical wise but future protective studies in which 30 mg/kg doses can be used safely is encouraged.
Serum Biochemical Profile and Protein Fractions in Cattle With Theileriosis
(Ecole Nationale veterinaire Toulouse, 2014) Dede, S.; Altug, N.; Deger, Y.; Ozdal, N.; Ceylan, E.
Theileriosis encountered frequently in Van region and causes huge economic losses in cattle, sheep and goats. This study was carried out to examine the serum biochemical profile and serum protein fractions, determined before and 7 days after buparvaquone treatment, in 28 cattle, diagnosed to be infected with T. annulata and in 15 healthy control animals from the same region. The ALT (alanine amino transferase) and ALP (alkaline phosphatase) activities and the concentrations of bilirubin and urea were markedly increased in sera from infected animals whereas the concentrations of glucose, minerals (Na, K and Ca), total proteins, albumin and of a-globulins and the albumin/globulin (A/G) ratios were significantly depressed compared to the controls. In addition, buparvaquone treatment succeeded in alleviating the serum biochemical anomalies. These results show that liver and kidney failures occur during theileriosis leading to a global protein deficiency and that serum biochemistry and serum protein analysis may allow biochemical evaluation of treatment.
Serum Copper, Zinc, and Calcium Concentrations in Lice-Infested Sheep
(2002) Deger, Y.; Dede, S.; Deger, S.
The purpose of this study was to investigate changes in serum concentration of copper, zinc, and calcium in sheep naturally infested with lice (Bovicola caprae, Linognathus africanus, Linognatus ovillus, and Linognattus pedalis). Twenty sheep naturally infested with lice and 20 healthy sheep were used as subjects. Blood samples were collected from the sheep before and 8 and 15 d after treatment with Avermectin, a veterinary antiparasitic drug. The samples were analyzed for their serum copper, zinc, and calcium concentrations by atomic absorption spectrometry. The concentrations of these elements in the infested animals were lower than in the healthy controls, mainly because the general condition of the affected sheep was poor. When the infested animals were treated with an ectoparasitic drug, the serum levels of the studied elements rose to normal ranges while the health of the animals improved.
Serum Protein Fraction Profile in Sheep Infected With Sarcoptes Ovis
(Ataturk Universitesi, 2020) Çetin, S.; Usta, A.; Ekici, P.; Dede, S.; Yüksek, V.
Scabies is an ectoparasitic infestation that is rapidly transmitted to sheep of any age. The present study was conducted to identify the significance of serum protein fractions in the diagnosis and treatment of animals infected by Sarcoptes ovis. The study group included 1-4 years old 18 Akkaraman sheeps with excessive itching and molting and infected with natural Sarcoptes ovis scabies and the control group included 1-4 years old 18 healthy sheeps, a total of 36 sheeps were included in the present study. Serum protein fractions were determined with the cellulose-acetate electrophoresis method in collected blood samples. There were no significant differences between total protein and α-2 globulin serum protein concentrations in the study and control groups. Although α-1, β-globulin and albumin concentrations were statistically higher in the patient group, gamma globulin levels decreased in the patient group (P<0.05). While the percentage (%) g protein did not differ based on α-2 globulin when compared to control, α-1, beta globulin, albumin and A/G ratio statistically increased in the patient group when compared to the control (P<0.05). There was a significant decrease in gamma globulins in the patient group when compared to the control (P<0.01). It was concluded that monitoring the serum protein parameters might be significant in the follow-up and treatment of scabies in sheep. © 2020 Ataturk Universitesi. All rights reserved.
The Serum Protein Fractions in Streptozotocin (Stz) Administrated Rat Models
(EManuscript Services, 2017) Ragbetli, C.; Dede, S.; Koc, F.; Yuksek, V.; Ragbetli, M.C.
Background: Experimental diabetes can be induced using chemical agents such as streptozotocin Objective: This study aimed to investigate the effect of streptozotocin (STZ) which is most important agent to produce experimental diabetic model at two different doses on serum protein fractions in rat models. Materials and Methods: Twenty-four male rats that weighed an average of 250 g and were 3-4 months old were used as the experimental models. They were sorted into three groups composed of eight rats each of STZ 55 mg/kg, STZ 65 mg/kg and control. Diabetes was induced by administering STZ 55 mg/kg and 65 mg/kg intraperitoneally. The serum protein fractions were analyzed by cellulose acetate electrophoresis. Results: No significant difference was observed between the groups for all fractions except alpha-2 and beta globulins. The alpha-2 and beta globulin levels were significantly higher in the 55 mg/kg group than in the 65 mg/kg STZ and control groups (p<0.05). Conclusion: This increase may be due to the involvement of different proteins in the alpha-2 and beta globulin protein fractions. © 2016 Phcog.Net.