The Effect of Vitamin C and Vitamin E Administration for Treatment and Protection on Serum Vitamin Levels in Rats With Experimentally Fluorosis Created

Abstract

This study aimed to determine the vitamins A-D and E levels in rats with experimental fluorosis and in whom vitamins, C and E were administered for prevention and treatment. One-month-old Wistar-Albino rats were used as material in the study. Nine groups of 8 animals each were studied. Sodium fluoride (NaF) solutions (150 mg/kg) prepared in drinking water were administered ad-libitum during the study. Vitamin C (100 mg/kg), Vitamin E (300 mg/kg) and Vitamin C + Vitamin E (100 mg/kg + 300 mg/kg) were administered to the protection group with water containing 150 ppm NaF for 16 weeks/day. Water containing 150 ppm NaF was given ad-libitum for 16 weeks in the treatment group, and then Vitamin C (100 mg/kg), Vitamin E (300 mg/kg) and Vitamin C + Vitamin E (100 mg/kg + 300 mg/kg) were administered along with normal drinking water for 4 weeks. At the end of sixteen weeks, blood samples were taken from the rats and their serums were removed. Serum Retinol, α-Tocopherol, Vitamin D3 levels were determined by HPLC. In the fluorosis group, it was found that there was no change in serum vitamin A and vitamin D levels with the application of vitamins C and E for treatment and protection, but a decrease in vitamin E level. As a result, it was concluded that the administration of vitamin E is effective in both the prevention and treatment of fluorosis cases. © 2021 Ataturk Universitesi. All rights reserved.