Browsing by Author "Deger, Yeter"

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The Concentration of Certain Trace Elements in The Wool of Sheep With Fluorosis
(int Soc Fluoride Research, 2020) Cetin, Sedat; Deger, Yeter; Dede, Semiha; Yur, Fatmagul
The aim of this study was to evaluate the trace element levels in the wool of sheep, with and without fluorosis, living in a volcanic area of Turkey. Fifteen Akkaraman sheep with fluorosis in the Agri region, to the north of Lake Van, and 10 Akkaraman sheep without fluorosis in the Van region, to the south of Lake Van in the eastern part of Turkey, were investigated. The urinary fluoride levels were measured with an ion selective electrode. The sheep with fluorosis were identified by clinical examination and the presence of a high urinary fluoride level. The wool samples were obtained from the neck region. The trace element concentrations in these samples were measured using an atomic absorption spectrophotometer. It was determined that in the fluorosis group, compared to the control group, the copper and zinc levels were significantly decreased (p=0.05), and a non-significant decrease (p=0.05) was present for the levels of the nickel, manganese, iron, and cobalt.
Concentration of Products of Nitric Oxide Oxidation and Some Vitamins in Sheep With Naturally Acquired Babesiosis
(Tubitak Scientific & Technological Research Council Turkey, 2009) Mert, Handan; Yoeruek, Ibrahim; Deger, Yeter; Mert, Nihat; Dede, Semiha; Yur, Fatmaguel
The aim of the present study was to determine serum concentrations of the products of nitric oxide oxidation (nitrate and nitrite) and some vitamins (retinol acetate, alpha- and delta-tocopherol, and vitamin D 3) in sheep naturally infected with Babesia ovis. The investigation included 30 infected and 10 control sheep. Serum a- tocopherol levels were significantly lower (P < 0.05), and nitrate and nitrite concentrations were significantly higher (P < 0.05) in infected animals than in controls. It is thought that the elevated nitrate and nitrite levels of the sheep infected with babesiosis were due to the result of damage caused by Babesia. On the other hand, a significant decrease was observed in a- tocopherol levels in sheep with babesiosis because of damage and pathophysiological changes to erythrocytes.
Determination of the Levels of Serum Oxidative Indicator, Cytokine and Some Biochemical Parameters in Horses Naturally Infected With Theileria Equi
(Ankara Univ Press, 2020) Ozdek, Ugur; Oguz, Bekir; Komuroglu, Ufuk; Deger, Yeter
Equine theileriosis is considered as a serious problem because of harmful effects on the health and performance of equids. Despite the importance of this disease, there are few studies in related to clinical pathologic changes in equine theileriosis especially in horses. In this study, the oxidative stress biomarkers, cytokines, enzymes, lipid profile, electrolytes, minerals and some metabolites were evaluated in horses naturally infected with Theileria equi (n=22) and healthy (n=7). In infected horses, the serum concentrations of MDA, IFN-gamma, total protein, globulin, bilirubin (total, direct, indirect), triglyceride, glucose, iron, chloride, sodium and copper with enzyme activities of ALP, AST and GGT were found significantly higher, however concentrations of GSH, albumin, total cholesterol, HDL, TIBC, calcium, phosphorus, magnesium, potassium, zinc and manganese with enzyme activity of CAT were found lower when compared to the healthy horses (P<0.05). The changes in TNF-alpha, creatinine, urea and LDL concentrations were not statistically significant (P>0.05).The results indicate that there are significant changes in the oxidative indicator, cytokine, and biochemical parameters of horses in T. equi infection and that these changes may be useful in the evaluation of the diagnosis, prognosis and treatment of theileriosis. In addition, comprehensive studies are needed to better understand the role of cytokines in the pathogenesis of theileriosis.
The Effect of Chitosan on the Erythrocyte Antioxidant Potential of Lead Toxicity-Induced Rats
(Humana Press inc, 2018) Toz, Hasan; Deger, Yeter
In the present study, the effects of chitosan on erythrocyte malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH) enzyme activities in lead toxicity-induced rats were investigated. Twenty-eight male Wistar albino rats were divided into four groups of control (C), lead group (Pb group), lead + chitosan group (Pb + CS group), and chitosan group (CS group). Lead groups were administered 50 mg/kg lead acetate intraperitoneally (ip) for 5 days and chitosan groups were administered 200 mg/kg chitosan for 28 days via gavage. At the end of the study, lead levels were measured in the blood; MDA and GSH levels and GPx, GR, and G6PDH activities were measured in the erythrocyte. It was determined that, in parallel with the increase of full blood lead levels in the Pb group, erythrocyte MDA levels increased significantly, while GSH levels and GSH-Px, GR, and G6PDH activities decreased when compared to those in the C and CS groups (p E, 0.05). There was a statistically significant decrease in lead and MDA levels and GSH level and GSH-Px activity increased (p E, 0.05) in the Pb + CS group, where chitosan was administered as a protective agent in addition to lead, when compared to the Pb group. There were no differences between the Pb + CS group and the other three groups based on GR and G6PDH activities (p E integral 0.05). No statistically significant difference was found between the C and CS groups based on the parameters of analysis (p E integral 0.05). The findings of the present study demonstrated that lead increased oxidative stress by increasing free radical production in erythrocytes, and chitosan was effective in removing the lead from the circulation and enforced the antioxidant defense system.
The Effect of Diplotaenia Turcica Root Extract in Streptozotocin-Induced Diabetic Rats
(Walter de Gruyter Gmbh, 2020) Ozdek, Ugur; Yildirim, Serkan; Deger, Yeter
Background: Diplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated. Materials and methods: In this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50,100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests. Results: It was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT. Conclusion: These results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.
The Effect of Glutathione Treatment on the Biochemical and Immunohistochemical Profile in Streptozotocin-Induced Diabetic Rats
(Springer, 2013) Yur, Fatmagul; Dede, Semiha; Karaca, Turan; Yegin, Sevim Ciftci; Deger, Yeter; Ozdemir, Hulya
This study investigated the possible role of glutathione (GSH) in diabetic complications and its biochemical safety in experimental diabetic rats. Serum biochemical parameters and the histology of the pancreas were investigated. Seven rats were separated as controls. To create the diabetes in rats, 45 mg/kg single-dose streptozotocin (STZ) was administered i.p. The treatment was continued for 1 month. STZ was administered to the diabetes + GSH group, then reduced GSH, dissolved in isotonic salt solution (200 mg/kg), was applied i.p. two times a week. The GSH group received i.p. GSH. Serum biochemical parameters were determined by autoanalyzer. Immunohistochemical procedures were used to determine the percentage of the insulin-immunoreactive beta-cell area in the islets of Langerhans. The biochemical parameters changed to different degrees or did not change. Pancreatic cells of the control and GSH groups were healthy, but in the diabetic and GSH-treated diabetic groups we found damage in different numbers. The results from these analyses show that GSH supplementation can exert beneficial effects on pancreatic cells in STZ-induced diabetic rats and can safely be used for therapy in and protection from diabetes and complications of diabetes.
Effects of 900-Mhz Electromagnetic Field Emitted From Cellular Phone on Brain Oxidative Stress and Some Vitamin Levels of Guinea Pigs
(Elsevier, 2007) Meral, Ismail; Mert, Handan; Mert, Nihat; Deger, Yeter; Yoruk, Ibrahim; Yetkin, Aysen; Keskin, Siddik
This study was designed to demonstrate the effects of 900-MHz electromagnetic field (EMF) emitted from cellular phone on brain tissue and also blood malondialdehyde (MDA), glutathione (GSH), retinol (vitamin A), vitamin D3 and tocopherol (vitamin E) levels, and catalase (CAT) enzyme activity of guinea pigs. Fourteen male guinea pigs, weighing 500-800 g were randomly divided into one of two experimental groups: control and treatment (EMF-exposed), each containing seven animals. Animals in treatment group were exposed to 890- to 915-MHz EMF (217-Hz pulse rate, 2-W maximum peak power, SAR 0.95 w/kg) of a cellular phone for 12 h/day (11-h 45-min stand-by and 15-min spiking mode) for 30 days. Control guinea pigs were housed in a separate room without exposing EMF of a cellular phone. Blood samples were collected through a cardiac puncture and brains were removed after decapitation for the biochemical analysis at the end of the 30 days of experimental period. It was found that the MDA level increased (P < 0.05), GSH level and CAT enzyme activity decreased (P< 0.05), and vitamins A, E and D3 levels did not change (P > 0.05) in the brain tissues of EMF-exposed guinea pigs. In addition, MDA, vitamins A, D3 and E levels, and CAT enzyme activity increased (P < 0.05), and GSH level decreased (P < 0.05) in the blood of EMF-exposed guinea pigs. It was concluded that electromagnetic field emitted from cellular phone might produce oxidative stress in brain tissue of guinea pigs. However, more studies are needed to demonstrate whether these effects are harmful or/and affect the neural functions. (C) 2007 Elsevier B.V. All rights reserved.
Effects of Ellagic Acid on Levels of Sialic Acid and Cardiac Markers in Rats With Myocardial Infarction
(Parlar Scientific Publications (p S P), 2021) Taskin, Ergin; Deger, Yeter
It has been shown that ellagic acid (EA) normalizes the activities of antioxidative enzymes, decreases the levels of inflammatory cytokines, and modulate the expression of apoptotic protein. The aim of this study was to evaluate the effect of EA on levels of sialic acid (SA) and cardiac markers in isoproterenol (ISO)-induced MI in rats. 32 female Wistar-albino rats were divided into 4 groups each containing 8 rats: group I (healthy control group), group II (ISO group), group III (EA ISO group), and group IV (EA group). Serum total SA (TSA). lipit-bound SA (LSA), and creatine kinase-myocardial band (CK-MB) levels were lower in EA+ISO group than in ISO group. Remarkably. serum LSA levels showed a significant positively correlation with all cardiac markers (CKMB, cardiac troponin I (cTnI), and lactate dehydrogenase (LDH)). In the histopathological examination, there was less degeneration in myocardial tissue of the rat in EA+ISO group than in those of ISO group. This study shows that EA has a protective effect in ISO -induced MI in rats. Also, it is seen that serum levels of SA, especially LSA, can be used as a cardiac damage marker.
Effects of Green Tea on Electrocardiography of Guinea Pigs Exposed To Electromagnetic Field Emitted by Mobile Phones
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Kilicalp, Dide; Deger, Yeter; Cinar, Ali
This study was carried out to determine the effects of green tea on Electrocardiography (EKG) of Guinea pigs exposed to a 900 MHz electromagnetic field emitted by mobile phones. Because green tea has a beneficial effect on endothelial function, tea consumption is associated with decreased cardiovascular risk. Twenty-eight healthy guinea pigs weighing 600-800 g were used. After one week adaptation period, animals were randomly divided into four groups. Four experimental groups labeled as controls (Group A), irradiated (Group B), irradiated receiving green tea extract (Group C) and green tea only (Group D) were formed with seven randomly chosen animals. Group A and D were housed in a separate room without exposing EMF of mobile phones. Irradiation was accomplished by exposing the animals in Group B and C to a 900 MHz electromagnetic field from a 217-Hz pulse rate, 2-W maximum peak power mobile phone placed in the cage were the animals were kept. ECG of guinea-pigs in four groups was recorded by a direct writing electrocardiograph at the end of the 30-day experimental period during exposure to mobile phone. Sinus bradycardia (lower heart beat) and prolongation of the P-R interval was observed in Group B and C after exposure to electromagnetic field (P<0.05). Whereas the heart rate in the green tea extract supplemented group was close to the control group's level, it was found to have been the lowest in the only irradiated (Group B). We found measurable effects in the heart rate and P-R interval parameters in the EM-exposed guinea pigs. It was found that EMF exposure for 30 days have some effects on ECG findings of guinea-pigs. The results suggest that exposure of guinea pigs to EM fields can cause disturbances in autonomic cardiac regulation.
Effects of Vitamin E and Selenium on Serum Trace and Major Elements in Horses
(Humana Press inc, 2008) Yur, Fatmaguel; Dede, Semiha; Deger, Yeter; Kilicalp, D.
The combined effects of vitamin E and selenium were studied in native Anatolian horses subject to strenuous exercise. The concentrations of copper, zinc, iron, calcium, potassium, and magnesium were determined in serum by atomic absorption spectrometry in two study groups (n=25 each), one of which served as untreated controls. After exercising the horses by running 1,500 m in about 7 min, only the copper level and the copper/zinc ratio significantly increased (p<0.05), but the concentrations of calcium, potassium, iron, and magnesium remained unchanged. In horses treated with vitamin E and selenium, the calcium and potassium levels decreased to levels lower than those of untreated controls before and after exercise. The iron levels were not changed by exercise or treatment alone but increased when the horses had been supplemented and exercised. The copper level and the copper/zinc ration increased as a result of exercise in both treated and untreated horses. These changes suggest that supplementation with vitamin E and selenium had an important effect on the serum concentrations of calcium, potassium, copper, iron, and the copper/zinc ratio.
Effects of X-Ray Radiation on Oxidation Products of Nitric Oxide in Rabbits Treated With Antioxidant Compounds
(Walter de Gruyter Gmbh, 2009) Dede, Semiha; Deger, Yeter; Kahraman, Tahir; Kilicalp, Dide
Objectives: The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the nitric oxide oxidation products nitrite and nitrate in rabbits after exposure to X-rays. Methods: The animals were divided into two experimental, one control group. The vitamin-supplemented group was given daily oral doses of vitamins E and C. Supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the mineral-supplemented group. Blood samples were taken from all groups before and after 4 weeks of vitamin and mineral administration and after irradiation with 550-rad X-rays. The nitric oxide oxidation products levels were analyzed in blood serum. Results: After irradiation, the control group showed increased levels of nitric oxide oxidation products (p<0.05). In the vitamin group, the concentration of nitric oxide oxidation products was lower (p<0.05) when compared to controls. In the MG, the mineral treatment was not affected nitric oxide oxidation products, but, irradiation was increased nitrate levels. Conclusion: It is concluded that the elevated levels of nitrate and nitrite concentrations in serum is the result of damage caused by the x-ray radiation. The results suggest that supplementation with antioxidant vitamins and minerals may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by X-rays.
Effects of Α-Tocopherol on Serum Trace and Major Elements in Rats With Bleomycin-Induced Pulmonary Fibrosis
(Humana Press inc, 2006) Dede, Semiha; Mert, Handan; Mert, Nihat; Yur, Fatmagul; Ertekin, Ali; Deger, Yeter
The study was undertaken to investigate the influence of a-tocopherol on zinc, copper, iron, calcium, magnesium, and potassium concentrations in serum of rats with bleomycin-induced pulmonary fibrosis. Fourteen Wistar albino rats were randomly divided into two groups of seven animals each. The first group was treated intratracheally with bleomycin hydrochloride (BM group); the second group was also instilled with BM but received injections of alpha-tocopherol twice a week (BM+E group). The third group was treated in the same manner with saline solution only, acting as controls (C). The zinc concentrations of the BM and BM+E groups were significantly decreased compared to the controls (p < 0.05). The iron concentration of the controls was significantly higher than the other two groups. The magnesium concentration in the controls and the BM+E group was significantly higher than that of the BM group. The serum copper, calcium, and potassium concentrations were not found to be statistically different among the three groups. Distinct histopathologic changes were found in the BM group compared to the untreated rats. Less severe fibrotic lesions were also observed in the BM+E group. The results of this study show that lungs of rats treated with bleomycin were seriously damaged and that vitamin E seemed to counteract some of the damage, as indicated by differences in the serum concentrations of major elements.
Evaluation of Oxidative Stress, Thyroid Hormones, Trace Elements and Some Biochemical Markers in Goats Naturally Infected With Theileria Ovis
(Springer int Publ Ag, 2024) Caglar, Yunus Emre; Deger, Yeter; Oguz, Bekir
Background Theileriosis is a tick-borne disease caused by protozoon species in the Theileria genus of the Theileriidae family. The biochemical changes induced by infection are considered to be an important understanding of the pathophysiology of caprine theileriosis. In this study, it was aimed to determine oxidative stress, thyroid hormones, trace elements, and biochemical parameters in theileriosis infection. Materials and Methods A sample of 14 goat was used for this purpose, of which 7 were healthy and 7 were infected with Theileria ovis. Theileria infection was diagnosed from the polymerase chain reaction (PCR). Sera from blood samples was tested for total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), alanine aminotransferase (ALT), aspartate transaminase (AST), gamma glutamyl transferase (GGT), total protein, albumin, triglyceride, cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), urea, blood urea nitrogen (BUN), creatinine, triiodothyronine (T3), thyroxine (T4), copper (Cu), zinc (Zn), cobalt (Co), manganese (Mn), selenium (Se), iron (Fe). Result TOC, OSI, AST, ALT and GGT values were higher in the patient group than in the healthy group (P < 0.05). On the other hand, there were decreases in TAC, T3, T4, total protein, albumin, creatinine, Cu, Zn, Se, and Co values (P < 0.05). However, there was not found to be a statistical difference between the healthy and patient groups in terms of triglyceride, cholesterol, HDL, LDL, urea, BUN, Mn, and Fe values (P > 0.05). Conclusions It can be stated that oxidative stress is a complication of caprine theileriosis and it may be accompanied with hypothyroidism and deficits in trace minerals.
Evaluation of Serum Lipoprotein and Tissue Antioxidant Levels in Sheep With Fluorosis
(int Soc Fluoride Research, 2013) Yur, Fatmagul; Mert, Nihat; Dede, Semiha; Deger, Yeter; Ertekin, Ali; Mert, Handan; Isik, Alper
The aim of this study was to evaluate serum lipoprotein and tissue antioxidant levels of sheep with and without fluorosis living in a volcanic area of Turkey. Fifteen Akkaraman sheep with fluorosis in the Agri region north of Lake Van and 10 Akkaraman sheep without fluorosis in the Van region just south of Lake Van in the eastern part of Turkey were investigated. In the kidney tissues, the MDA levels and SOD activities in the fluorosed sheep showed nonsignificant increases, but the GSH level and GPx activities significantly decreased. In the liver tissues of the fluorosed sheep, a significant increase in the MDA level and GPx activity was observed, but the GSH level showed no change, and the SOD activity exhibited a small decrease. By contrast, in the muscle tissues, the MDA level decreased and the SOD activity increased significantly, whereas the GPx activity increased but not significantly, and the GSH levels deoreased. Finally, the serum lipoprotein levels of the fluorosed and nonfluorosed sheep were not significantly different. In conclusion, different degrees in the pro-oxidant/antioxidant status of soft tissues such as kidney, liver, and muscle were affected by F intoxication, but no differences were found in the serum lipoprotein levels.
Investigating Erythrocyte Membrane Lipid and Protein Oxidation With Na+ Activity in Caprine Anaplasmosis
(Sivar-soc Italiana veterinari Animali Reddito, 2020) Ilkaya, Salim; Deger, Yeter; Oguz, Bekir; Ozdek, Ugur
Anaplasmosis is an infectious disease that is caused by the genus Anaplasma belonging to the family Anaplasmataceae in the order Rickettsiales and is seen in the mammals inhabiting tropical and subtropical climate zones. Anaplasma ovis and A. phagocytophilum are the most remarkable species causing anaplasmosis in goats. Anaplasmosis induces both cellular and humoral immunity. As animals develop a long-term immunity against the disease, it becomes difficult to treat the disease. Immunity develops mainly depending on premunition. Increased osmotic fragility of erythrocytes in animals infected with anaplasmosis has been found, and it has been reported that this may be developed by various immune-mediated mechanisms including oxidative damage. Moreover, it may also be associated with high cell membrane ATPase activity and erythrocyte morphological changes. The aim of this study was to evaluate the effect of anaplasmosis on erythrocyte membrane malondialdehyde (MDA), advanced oxidation protein products (AOPP), Sodium-potassium adenosine 5'-triphosphatase (Na+/K(+)ATPase), and hematological and biochemical parameters in goats. For this purpose, 45 male hair goats (35 infected and 10 healthy) that were brought to the slaughterhouse of Van Metropolitan Municipality were enrolled in the study. In infected goats, the hematological and biochemical concentrations of RBC, Hb, Hct, MCV, MCH, MCHC as well as serum total protein, albumin, total cholesterol and TIBC were found significantly lower, however concentrations of of WBC, globulin, total bilirubin, direct bilirubin, triglyceride, and iron as well as enzyme activities of AST, ALT, GGT were found higher when compared to the healthy goats (p<0.05). In addition, the MDA and AOPP levels were markedly increased in erythrocyte membrane from infected animals while the Na+/K(+)ATPase enzyme activity was significantly decreased compared to the the healthy goats (p<0.05). Therefore, it can be concluded that oxidative stress in erythrocyte membrane may play an important role in the pathogenesis of anemia in caprine anaplasmosis.
Investigation of Blood Antioxidant Enzyme Levels and Glutathione Peroxidase, Catalase, and Superoxide Dismutase Gene Polymorphism in Sheep With Fluorosis
(int Soc Fluoride Research, 2017) Ozbey, Ulku; Deger, Yeter; Yur, Fatmagul; Cambay, Zafer; Ozbey, Gokben
Reactive oxygen species have been considered to play a significant part in the development of fluorosis, a critical public health issue in many parts of the world. OBJECTIVES: The aims of this study, conducted on two groups of sheep, with and without fluorosis, was (i) to determine the effect of fluorosis on antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and on malondialdehyde (MDA); and (ii) to investigate the relationship between the SOD, CAT, and GSH-Px genotypes and the antioxidant enzyme levels. METHODS: The two groups of sheep were: (i) 24 Akkaraman sheep with fluorosis living in Dogubeyazit province of Agri and (ii) 20 Akkaraman sheep with no signs of fluorosis living in Van. The MDA level and the antioxidant enzyme activities were identified spectrophometrically. The genotype characteristics were determined by the PCR-RFLP method using DNA extracted from blood. RESULTS: Compared to the control sheep without fluorosis, we found in the fluorotic sheep: (i) an increase in plasma MDA levels and GSH-Px-1 activity (p<0.05), and (ii) a decrease in SOD and CAT activities (p< 0.01). There were significant differences found in the genotype and allele frequencies in both groups (p<0.01). CONCLUSIONS: Fluoride-induced oxidative stress affected the membrane structure resulting in elevated erythrocyte MDA levels and reduced enzyme activity of the enzymes SOD and CAT. According to our data, the genepolymorphisms show a tendency towards fluorosis for our local sheep population.
Investigation of the Effects of Diplotaenia Turcica Plant Root Extract on Rat Over Tissue by Histological and Stereological Methods
(Parlar Scientific Publications (p S P), 2019) Colcimen, Nese; Ozdek, Ugur; Deger, Yeter; Altindag, Fikret; Arihan, Okan
Diplotaenia turcica is a plant species which is found in Bitlis-Van-Hakkari region and used in traditional treatments due to its medical and protective activities. Aim of this study is to investigate effects of root extract of Diplotaenia turcica plant on rat over tissue with histological and stereological methods. Four groups, with seven rats each, were used in experiments. Experimental groups were as follows; (I): control group, standard food and water was administered, Diplotaenia turcica plant root extract groups; (II): 250 mg/kg, (III): 550 mg/kg, (IV): 1000 mg/kg were administered as single dose/day with gastric gauge for 28 days. At the end of the experiments rats were decapitated and right over tissue was obtained. Histologically an increase in over medulla structure and an increase in corpus luteum and Graaf follicle number in cortex in plant administered groups were observed. Total volume comparison showed no difference between groups (p>0.05). When follicle numbers were evaluated Diplotaenia turcica administered groups showed a significant decrease in Primordial follicle number compared to control (p<0.05). No change in Primary follicle number (p>0.05) and an increase in Graaf follicle number (p<0.05) was observed. These results showed that Diplotaenia turcica increases follicular maturation by acting on this process.
Investigation of the Effects of Α-Tocopherol on the Levels of Fe, Cu, Zn, Mn, and Carbonic Anhydrase in Rats With Bleomycin-Induced Pulmonary Fibrosis
(Humana Press inc, 2007) Ertekin, Ali; Deger, Yeter; Mert, Handan; Mert, Nihat; Yur, Fatmagul; Dede, Semiha; Demir, Halit
This study was designed to examine the effects of vitamin E on the levels of Zn, Mn, Cu, Fe, and carbonic anhydrase in rats with bleomycin-induced pulmonary fibrosis. Twenty-one male Wistar albino rats were randomly divided into three groups: bleomycin alone, bleomycin+vitamin E, and saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single dose) bleomycin hydrochloride intratracheally. The bleomycin+vitamin E group was also instilled with bleomycin hydrochloride but received injections of a-tocopherol twice a week. The control group was treated with saline alone. Animals were sacrified 14 d after intratracheal instillation of bleomycin. Tissue Zn, Mn, Cu, Fe, and carbonic anhydrase activities were measured in the lung and liver. Lung Cu, Fe, and carbonic anhydrase activity increase in both experimental groups. Zn and Mn levels decreased, except for the Mn level in the bleomycin group. Liver Zn, Mn, and Cu levels decreased in both experimental groups compared to the control group, whereas Fe and carbonic anhydrase activity increased in comparison to the control group. However, the liver tissue Fe level decreased compared to the control group. In the histopathologic assesment of lung sections in the bleomycin+vitamin E group, partial fibrotic lesions were observed, but the histopathologic changes were much less severe compared to the bleomycin-treated group.
Na+ Activity in Sheep With Natural Babesiosis
(veterinarni A Farmaceuticka Univerzita Brno, 2010) Yur, Fatmaguel; Yazar, Mete; Deger, Yeter; Dede, Semiha
The aim of this study was to determine the Na+/K(+)ATPase activity in the erythrocytes of sheep naturally infected with Babesia ovis before and after treatment. Seven diseased sheep and seven control animals were used for the study. Babesia infection was confirmed with Giemsa's staining of blood smears. Na+/K(+)ATPase activity in erythrocyte was determined colorimetrically by the release of inorganic phosphate from ATP in the presence and absence of ouabain. A marked decrease of Na+/K(+)ATPase activity (p < 0.05) was obtained in infected sheep (0.81 +/- 0.28 mu mol inorganic phosphate/h.mg protein) compared to control animals (3.63 +/- 0.72 mu mol Pi/h.mg protein). Beside this decline, Na+/K(+)ATPase activity of treated animals (1.29 +/- 0.81 mu mol Pi/h.mg protein) were significantly (p < 0.05) decreased compared to control animals. It can be concluded that decreased erythrocyte Na+/K(+)ATPase activity in babesia ovis may be due to the usage of new permeation pathways (NPPs) for the flux of ions instead of Na+/K+ pump.
Plasma Levels of Zinc, Copper, Copper/Zinc Ratio, and Activity of Carbonic Anhydrase in Equine Piroplasmosis
(Humana Press inc, 2008) Dede, Semiha; Deger, Yeter; Deger, Serdar; Tanritanir, Pinar
We have determined the plasma concentrations of copper, zinc, copper/zinc ratio, and carbonic anhydrase activity in horses infected with Babesia equi. The study was conducted in 14 horses with the disease and 10 healthy animals that served as controls. The infection was confirmed by the clinical manifestations of the disease and by Giemsa staining of thin blood smears showing the parasites inside red blood cells. The horses with piroplasmosis had lower plasma levels of zinc, elevated copper, and increased activity of carbonic anhydrase. Consequently, the copper/zinc ratio was also higher than in the healthy controls.