Browsing by Author "Ercan, Kerem"

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Determination of the Prevalence and Parasite Burden of Coeunurus Cerebralis and Oestrus Ovis in Sheep in Siirt Province of Türkiye
(veterinarni A Farmaceuticka Univerzita Brno, 2025) Kara, Murat; Celik, Burcak Aslan; Celik, Ozgur Yasar; Ayan, Adnan; Selcuk, Muhammed Ahmed; Ercan, Kerem; Ayan, Ozge Oktay
The aim of this study was to determine the prevalence and parasite burdens of Coeunurus cerebralis and Oestrus ovis in sheep in Siirt province, T & uuml;rkiye. Between December 2022 and November 2023, a total of 520 sheep (260 male, 260 female) heads (10 heads per week) were randomly sampled from a butcher. The total prevalence of C. cerebralis was determined to be 12.9%. The prevalence was higher in males, in the Morkaraman breed, in animals younger than two years of age, in dark-coloured ones, in October, and in the right brain hemisphere. The total prevalence of O. ovis was determined to be 38.3%. The prevalence was higher in females, in the Akkaraman breed, in dark-coloured breeds, in age groups older than two years, and in December. In terms of larvae, L1 was detected in 108 sheep, L2 in 106 sheep, and L3 in 139 sheep. A total of 1,039 larvae (278 L1, 321 L2, and 440 L3) were detected. The significant presence of O. ovis found in the sheep in this study indicates that Siirt province has suitable climatic conditions for O. ovis and larval development. It is recommended to include more animals in a future study in order to determine the exact seasonal prevalence of O. ovis infestation in the region, and to use an antiparasitic drug effective against O. ovis in the routine treatment of sheep to reduce the incidence of this disease.
Microscopic and Molecular Prevalence of Cryptosporidium Spp. in Lambs in Siirt, Turkey
(Natl information Documentation Centre, 2023) Celik, Ozguer Yasar; Celik, Burcak Aslan; Ayan, Adnan; Kilinc, Oezlem Orunc; Ercan, Kerem; Selcuk, Muhammed Ahmed; Ayan, Oezge Oktay
CRYPTOSPORIDIOSIS is a zoonotic disease that occurs acutely or chronically in young or immune-compromised animals and humans, caused by Cryptosporidium species. Cryptosporidium is recognized as one of the major enteropathogens associated with neonatal diarrhea in ruminants. The aim of this study is to determine the prevalence of Cryptosporidium spp. in lambs in Siirt province using microscopic and molecular methods. The fecal materials of the study collected from 194 randomly selected lambs of different sexes, up to 4 weeks of age, in various farms. As a result of microscopic examination, Cryptosporidium spp. oocysts were found in 54 (27.84%) of 194 samples, while specific bands were obtained in 63 (32.47%) samples as a result of Nested PCR analysis. A statistically significant relationship was found between lambs with and without diarrhea (P<0.001) while no statistically significant relationship was found between age groups, gender, and locations (P>0.05). In conclusion, the data obtained from this study revealed that Cryptosporidium infection is present in Siirt province, and Cryptosporidium spp. should be considered as one of the agents in the etiology of neonatal diarrhea in lambs.
Molecular Identification and Phylogenetic Analysis of Babesia Ovis in Sheep in Siirt, Turkiye: Relationship With Some Oxidant/Antioxidant Parameters
(veterinarni A Farmaceuticka Univerzita Brno, 2024) Aslan Celik, Burcak; Kara, Murat; Celik, Ozgur Yasar; Irak, Kivanc; Selcuk, Muhammed Ahmed; Ercan, Kerem; Ayan, Adnan
The Babesia genus includes tick-borne haemoprotozoan parasites that infect a wide variety of vertebrate hosts, both domestic and wild, around the world. Babesia spp. cause oxidative stress by increasing the number of free radicals in erythrocytes. Among this genus, Babesia ovis causes babesiosis in sheep and goats, especially in tropical and subtropical regions, causing significant economic losses. In this study, we aimed to determine the presence and prevalence of B. ovis in sheep in Siirt province, T & uuml;rkiye, using molecular method and to evaluate some oxidant/ antioxidant parameters in infected sheep. The animal material used in this study consisted of a total of 500 sheep. DNA extraction, Polymerase Chain Reaction (PCR) amplification and sequence analysis of blood samples and Enzyme-Linked ImmunoSorbent Assay (ELISA) analysis of serum samples were performed. As a result of the study, 84 of the 500 samples (16.80%) examined were PCR positive. Advanced oxidation protein products (AOPP) and nitric oxide (NO) concentrations were found to be higher in sheep with babesiosis compared to the control group. Superoxide dismutase (SOD) activity was higher in the control group and catalase (CAT) activity was higher in infected sheep. As a result of this study, the presence of Babesia ovis was detected in all districts of Siirt province. It has been determined that infection caused by Babesia ovis in sheep causes oxidative stress as a result of increased nitric oxide and oxidized protein levels and this process may participate in the pathology of the disease.
Molecular Prevalence of Giardia Duodenalis and Subtype Distribution (Assemblage E and B) in Calves in Siirt, Turkey
(Natl information Documentation Centre, 2023) Celik, Burcak Aslan; Celik, Ozguer Yasar; Ayan, Adnan; Akyildiz, Guerkan; Kilinc, Oezlem Orunc; Ayan, Oezge Oktay; Ercan, Kerem
GIARDIA duodenalis is a common intestinal protozoan parasite that infects domestic and wild mammals, birds, and humans, especially livestock, worldwide. Studies on the prevalence of Giardia duodenalis in calves in Turkey are very limited. The aim of this study was to determine the molecular prevalence and subtype distribution of Giardia duodenalis in calves in Siirt. The animal material of the study consisted of 100 calves of different breeds and sexes in Siirt province. Using a disposable latex glove, feces samples were taken from the rectum of each calf and put in separate fecal containers. Sex and age information was recorded for each sample collected. As a result, 5% and 9% positivity was detected by microscopic and nested-PCR methods, respectively. The prevalence was 9.68% in females and 7.89% in males (P>0.05). Prevalence by age groups was as follows: 11.32% in the 1-6 month group, 7.41% in the 7-12 month group, and 5% in the older than 12 months group (P>0.05). As a result of the sequence analysis of the five PCR-positive samples, 80% Assemblage E and 20% Assemblage B were detected. As a result of this study, in addition to Assemblage E, Assemblage B was detected as well, which also has zoonotic properties. This situation may pose a risk for breeders. To better understand the distribution of G. duodenalis assemblages in calves in Siirt, studies in larger herds are needed.
Preliminary Investigation of the Prevalence and Genotype Distribution of Cryptosporidium Spp., and Giardia Duodenalis in Cats in Siirt, Turkey
(Sciendo, 2023) Aslan Celik, Burcak; Celik, Ozgur Yasar; Ayan, Adnan; Akyildiz, Gurkan; Orunc Kilinc, Ozlem; Oktay Ayan, Ozge; Ercan, Kerem
Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis. Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the beta-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans.
Prevalence and Genotypes of Giardia Duodenalis in Shelter Dogs of Southeastern Türkiye
(Urmia Univ, 2023) Celik, Burcak Aslan; Celik, Ozgur Yasar; Kochan, Akin; Ayan, Adnan; Kilinc, Ozlem Orunc; Akyildiz, Guerkan; Ercan, Kerem
Giardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakir province being located in the southeastern Anatolia region of Turkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.(c) 2023 Urmia University. All rights reserved.