Browsing by Author "Eroglu, Mehmet"

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Association of DGAT1 and POU1F1 Gene Polymorphisms With the Milk Traits in the Cross-Bred Hamdani Sheep Bred Under Extensive Management
(Wiley, 2025) Turgut, Ali Osman; Koca, Davut; Eroglu, Mehmet
Background: Milk production and composition are affected by genetic and environmental factors. Among key genetic regulators, the POU1F1 and DGAT1 genes play significant roles. POU1F1 affects pituitary gland functions and hormone secretion, indirectly impacting milk production. DGAT1 is crucial for milk fat synthesis. Understanding genetic variations in these genes can enhance breeding strategies for improved milk yield and quality. Objective: This study investigates the relationship between POU1F1 and DGAT1 genes and milk composition in cross-bred Hamdani sheep. Methods: Blood and milk samples from 70 sheep were analysed for genetic markers using PCR-RFLP technique. Statistical analysis assessed the relationship between genotypes and milk composition. Results: Results showed that DGAT1 genotypes (CC and CT) were in Hardy-Weinberg Equilibrium (HWE), whereas POU1F1 genotypes were not. C and T allele frequencies were found 0.9 and 0.1 for DGAT1, respectively. On the other hand, the frequencies of C and T alleles were 0.52 and 0.48 for POU1F1, respectively. For DGAT1 gene, CT genotypes carrying ewes had higher milk fat compared to CC genotypes (p < 0.05). However, no differences were observed in milk solids-not-fat, protein and lactose content between genotypes (p > 0.05). Effect sizes were detected as 0.83 (large) for fat percentage comparison, 0.09 (small) for percentage of solids-not-fat and 0.06 (small) for percentage of protein and lactose. Association analysis did not perform for POU1F1 gene due to low sample size in CC genotype. Conclusion: These findings suggest that DGAT1 variations may influence milk fat content, whereas POU1F1's role remains unclear due to limited genotype variation. Further research is needed to clarify genetic influences of DGAT1 and POU1F1 genes on sheep milk in cross-bred Hamdani sheep.
Comparison of Serum Biochemical Parameters in Ketotic and Healthy Siirt Colored Mohair Goats During Early Lactation to Identify Potential Biomarkers of Lactation Ketosis
(Univ Zulia, Facultad Ciencias Veterinarias, 2025) Irmak, Mehmet; Turgut, Ali Osman; Eroglu, Mehmet; Irak, Kivanc; Tufan, Tuncay; Koca, Davut; Unver, Ali
This study aimed to compare serum biochemical parameters between ketotic and healthy Siirt colored mohair goats during early lactation, to diagnose subclinical lactation ketosis and identify potential biomarkers. A total of 77 female goats, aged 2 to 5 years and within 30 days postpartum, were evaluated under similar management conditions. The animals were classified into two groups based on serum beta-hydroxybutyric acid levels: subclinical lactational ketosis (n = 37) and health controls (n = 40). beta-hydroxybutyric acid levels were significantly elevated in the ketosis group (0.891 +/- 0.0141 mmolL-1) compared to the control group (0.595 +/- 0.0159 mmolL-1, P<0.001), confirming its diagnostic value. Other serum parameters did not show significant differences between the groups (P>0.05). On the other hand, effect size analysis revealed that glucose level decreased in does with subclinical lactational ketosis while cholesterol increased (large effect size). The study concluded that most liver and metabolic indicators remained within normal ranges, while glucose and cholesterol levels changed due to subclinical lactational ketosis. These data indicate that feeding based on pasture under extensive conditions may be insufficient to meet the energy requirements of does in lactation and emphasize the importance of early biochemical screening during lactation for effective management of metabolic disorders during early lactation stage in goats. Therefore, it is recommended the further studies to expand the knowledge of the effect of lactational ketosis on metabolic changes in goats.
An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep
(2024) Ayan, Adnan; Eroglu, Mehmet; Küçük, Mürsel; Gülendağ, Erman; Koca, Davut; Turgut, Ali Osman; Önen, Muhammed Furkan
Milk traits of sheep are affected by many environmental and genetic factors. These traits are quantitative traits and are determined by many genes. This study aimed to investigate polymorphisms of milk-related Fatty-acid synthase (FASN), Stearoyl-CoA desaturase (SCD1), and solute carrier 27A3 (SLC27A3) genes in cross-bred Hamdani sheep. Blood samples were collected from 100 healthy cross-bred Hamdani ewes from the jugular vein into K3-EDTA-containing tubes. Genomic DNA was extracted using a commercial DNA isolation kit. PCR products of FASN (275 bp), SCD1 (225 bp), SLC27A3-P1 (341 bp), and SLC27A3-P2 (319 bp) were subjected to restriction fragment length polymorphism (RFLP) analysis using SSil (AciI), Cfr13I, AluI, and Hpy188III the restriction enzyme, respectively. However, all gene regions were found to be monomorphic. In the study, only TT, AA, AA, and GG genotypes were detected for FASN, SCD1, SLC27A3-P1, and SLC27A3-P2, respectively. Allele and genotype frequencies were 1.00 for all genotypes and alleles. Although this study did not reveal favorable genotypes in FASN, SCD1, and SLC27A3 genes that can be used for milk traits, more comprehensive studies with larger sample sizes should be conducted to investigate polymorphisms in cross-bred Hamdani sheep.
Prevalence of Subclinical Pregnancy Toxemia and Its Effect on Metabolic Profile of Ewes: A Field Study
(2025) İrak, Kıvanç; Eroglu, Mehmet; Irmak, Mehmet; Koca, Davut; Turgut, Ali Osman; Keskin, İbrahim Halil; Ünver, Ali
This study investigated the prevalence of subclinical pregnancy toxemia (SPT) in pregnant ewes and its effects on biochemical parameters. A total of 114 pregnant ewes were included in the study, and the animals were classified into two groups: healthy and SPT, based on their serum β-hydroxybutyrate (β-HBA) levels. The findings demonstrated a significant increase in β-HBA levels as pregnancy progressed (first trimester: 0.45 mmol/L; second trimester 0.58 mmol/L; third trimester: 0.74 mmol/L), indicating an elevated risk of pregnancy toxemia in later gestational stages. The most notable difference was observed in triglyceride (TRIG) levels, which significantly increased in the SPT group, reaching 51.1 mg/dL (p < 0.05). Although the aspartate aminotransferase (AST) level was not changed statistically, large effect size (0.91) indicates an increase tendency of AST in SPT group. Similarly, total bilirubin (TBILC) levels were elevated in the SPT group with moderate effect size (0.61). No significant differences were found between groups in terms of glucose (GLUC), cholesterol (CHOL), total protein (TP), albumin (ALB), gamma-glutamyl transferase (GGT), or alanine aminotransferase (ALT) levels. These results suggest that SPT exhibits a subclinical nature and causes limited hepatic involvement during the early stages. In conclusion, TRIG levels emerge as a potential supportive biomarker in addition to serum β-HBA for the early diagnosis of subclinical pregnancy toxemia. Therefore, routine monitoring of these parameters may be beneficial for the early detection of SPT and for taking preventive measures before the development of clinical symptoms.