Browsing by Author "Firat, Mehmet"

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7-Acetoxyhorminone From Salvia Multicaulis Vahl. as Promising Inhibitor of 3-Hydroxy Coenzyme a (hmg-Coa) Reductase
(Mdpi, 2022) Yigitkan, Serkan; Ertas, Abdulselam; Salmas, Ramin Ekhteiari; Firat, Mehmet; Orhan, Ilkay Erdogan
3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is a key enzyme involved in cholesterol biosynthesis and one of the most important targets for the treatment of hypercholesterolemia. A limited number of studies on the HMG-CoA reductase inhibitory potential of natural products are available. Thus, in the current study, we aimed to test the HMG-CoA reductase inhibitory capacity of extracts from the roots and aerial parts of Salvia multicaulis Vahl., through activity-guided isolation. Our findings revealed that the root extract prepared with dichloromethane-acetone (1:1) showed the highest inhibition (71.97 +/- 0.37%) at 100 mu g/mL. The extract was then initially fractionated by column chromatography and the obtained fractions were monitored by thin layer chromatography. Fractions which were similar to each other were combined and a total of 15 fractions were obtained. Further conventional chromatographic studies were carried out on the active fractions. Based on these fractions, 10 known compounds, comprising 9 terpenes and 1 steroid derivative in total, were isolated and their structures were verified by a combination of IT-TOF-MS, and 1D and 2D NMR techniques. According to the enzyme inhibition data of the identified compounds, 7-acetoxyhorminone exerted the highest inhibition (84.15 +/- 0.10%, IC50 = 63.6 +/- 1.21 mu g/mL). The molecular docking experiments on 7-acetoxyhorminone and horminone indicated that both compounds strongly bind to the active site of the enzyme.
Allium Hoshabicum a New Species of A. Sect. Codonoprasum (Amaryllidaceae) From Van (Turkey)
(Magnolia Press, 2017) Firat, Mehmet
A new species, Allium hoshabicum (A. sect. Codonoprasum), is described from Hosab/ Van province, Turkey. From the morphological point of view, it appears to be similar to Allium huber-morathii, but it differs in several morphological features including scape height, outer tunics shape of bulb, inner tunics colour of bulb, leaf width, umbels size or number, and gynoecium features. A comprehensive description of this species is provided, including detailed illustrations and photographs, geographical distribution, habitat and ecology, vernacular names, and IUCN conservation status.
Allium Taiseba, a New Species of Allium Sect. Scorodon (Amaryllidaceae) From Van Province (Turkey)
(Wiley, 2025) Firat, Mehmet
A new species, Allium taiseba (A. sect. Scorodon), is described from Ho & scedil;ap (Xo & scedil;ap)/Van province, Turkey. It is morphologically similar to A. bingoelense, but it differs in several morphological features including inner tunic's colour of bulb, spathe length, tepal colours and size, stamen colour and length, ovary shape, style colour and length, capsule and seeds size. A comprehensive description of the new species is provided, including detailed photographs, geographical distribution map, habitat and ecology, vernacular names and IUCN conservation status.
Antioxidant and Antiradical Properties of Rhabdosciadium Anatolyi Flowers and Contents of Vitamin, Trace Element and Mineral
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2023) Bakir, Ahmet; Ekin, Suat; Firat, Mehmet
The aim of this study was to investigate the antioxidant and antiradical activity of the Rhabdosciadium anatolyi ( R. anatolyi) flowers, an endemic plant grown in the Eastern Anatolia Region of Turkey, by determining the level of vitamins E and C, mineral (Ca, Na, Mg, P, K), and trace elements (Mn, Zn, Cu, Fe, V, Cr, Mo, Co, Sr, Pb, Ti, Tl, Sn, Cd, As). Within the scope of the study, mineral and trace element analyzes were carried out by ICP-OES and AAS, Vitamin E by HPLC, Vitamin C, total phenolic content, total flavonoid, antioxidant capacity, hydrogen peroxide, DPPH, ABTS, superoxide, hydroxyl and hemolysis of erythrocytes with phenylhydrazine, radical scavenging activity of the R. anatolyi flowers methanol extract were determined spectrophotometrically. According to the results, a-tocopherol, ascorbic acid, phenolic content, flavonoid content and total antioxidant activity of the R. anatolyi flowers were 3,99 +/- 0,35 mu mol kg(-1), 346.27 +/- 6.51 mg 100 g(-1), 21.94 +/- 0.37 mg gallic acid g(-1), 5.60 +/- 0.13 mg quercetin g(-1) ve 29.65 +/- 0.26 mM ascorbic acid g(-1), respectively. Consequently, the mineral, trace element, vitamin E and vitamin C, total phenol and flavonoid levels in the R. anatolyi flowers were high, and the R. anatolyi flowers methanol extract effectively inhibited free radicals. From this point of view, it is thought that it can be used in the preventive treatment of many diseases that may be caused by free radical species and that these data will be a reference for other studies.
Appraisal of the Antimicrobial and Cytotoxic Potentials of Nanoparticles Biosynthesized From the Extracts of Pelargonium Quercetorum Agnew
(Imr Press, 2021) Dumlupinar, Berrak; Karatoprak, Gokce Seker; Firat, Mehmet; Akkol, Esra Kupeli
Aim: The aim of this study is the synthesis of nanosilver particles (AgNPs) from Pelargonium quercetorum Agnew. (Geraniaceae) and evaluation of the antimicrobial and the cytotoxic potential of AgNPs. Methods: The synthesized AgNPs were evaluated for antimicrobial and anticancer efficacy using the minimum inhibition concentration method and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay. Results: The AgNPs inhibited approximately 90% the growth of gram-positive Staphylococcus aureus and gram-negative Esh-erichia coli and yeast Candida albicans pathogens at a concentration of 500 mu g/mL. The synthesized AgNPs showed excellent toxicity in MCF-7 cells, and specifically, pq70 AgNP inhibited the growth of MCF-7 cells by 52% at a concentration of 3.125 mu g/mL. Conclusion: It was determined that the AgNPs, which had been synthesized from extracts that contained a high phenolic composition, were smaller in size, and showed high anticancer and antimicrobial properties.
Arabis Watsonii (Phdavis) Fkmey.:an Overlooked Cruciferous Species From Eastern Anatolia and Its Phylogenetic Position
(Pensoft Publishers, 2016) Ozudogru, Baris; Firat, Mehmet
Arabis watsonii (P.H.Davis) F.K.Mey. was initially reported as Thlaspi watsonii P.H. Davis in Flora of Turkey. Although F. K. Meyer transferred this species to Arabis L., this species has been overlooked and treated as Thlaspi L. in relevant literature for Flora of Turkey. In this study this species was evaluated using molecular (nuclear ITS and plastidic trnL-F sequences) and morphological data. Results clearly show that A. watsonii is sister to the A. hirsuta aggregate and its relatives. In conclusion, our results increased the number of known Arabis species in Turkey to 23. Furthermore, detailed description and distribution of the species are given and a new IUCN threat category for A. watsonii is proposed.
Astragalus Bahcesarayensis (leguminosae-Papilionoideae), a New Species of Section Alopecuroidei Dc. From Turkey
(Oxford Univ Press, 2008) Akan, Hasan; Firat, Mehmet; Ekici, Murat
Astragalus bahcesarayensis H. Akan, M. Firat & M. Ekici (Leguminosae-Papilionoideae) from Turkey is described and illustrated. It is compared with A. dipsaceus Bunge and A. panduratus Bunge. The geographical distributions of the new species and related species are mapped. (C) 2008 The Linnean Society of London.
Astragalus Miksensis Fırat (Fabaceae), a New Species in Section Hymenostegis From Van Province, Türkiye
(Magnolia Press, 2024) Firat, Mehmet
Astragalus miksensis F & imath;rat (sect. Hymenostegis Bunge), a new species is described from Bah & ccedil;esaray (Miks)/Van province, T & uuml;rkiye. The new species is morphologically similar to Astragalus zohrabi Bunge , from which it differs in several morphological features including stipules, leaves, peduncle, indumentum, inflorescence shape, flower, ovary, fruit and seed characters. A comprehensive description of Astragalus miksensis is provided, including detailed photographs, distribution map, habitat and ecology, vernacular names and IUCN conservation status.
Astragalus Nordizensis (Fabacaea), a New Species From Van Province (Türkiye) Belonging To Section Hymenostegis
(Wiley, 2024) Firat, Mehmet
Astragalus nordizensis Firat (sect. Hymenostegis Bunge), is described as a new species from Gurpinar district of Van province, Turkiye. From the morphological point of view, the new species appears to be most similar to Astragalus zohrabi Bunge and A. rubrostriatus Bunge, but it differs in several morphological characters including peduncle indumentum, inflorescence shape, leaf and flower, fruit and seed morphology, etc. A comprehensive description of this new species is provided, including detailed photographs, geographical distribution map, habitat and ecology, vernacular name and IUCN conservation status.
Bioguided Isolation of Secondary Metabolites From Salvia Cerino-Pruinosa Rech. F. Var. Cerino-Pruinosa
(Acg Publications, 2021) Ertas, Abdulselam; Cakirca, Hatice; Yener, Ismail; Akdeniz, Mehmet; Firat, Mehmet; Topcu, Gulacti; Kolak, Ufuk
In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemic species, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and tested for determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, and cupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolated from the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4-hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2-dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone (11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid (15), oleanolic acid (16), taraxasterol (17), lupenone (18), beta-sitosterol (19), and stigmasterol (20). Rosmarinic acid, which was obtained from the aerial parts, was found to be the best antioxidant compound among the isolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays (IC50: 1.20 +/- 0.04 mu g/mL, IC50: 1.74 +/- 0.06 mu g/mL, A(0.5): 1.22 +/- 0.02 mu g/mL, respectively). Chlorogenic and caffeic acids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidant activity in the mentioned assays.
Biological and Chemical Comparison of Natural and Cultivated Samples of Satureja Macrantha Camey
(Acg Publications, 2021) Akdeniz, Mehmet; Yener, Ismail; Ertas, Abdulselam; Firat, Mehmet; Resitoglu, Baris; Hasimi, Nesrin; Oksuz, Sevil
In this study, investigation on the essential oils and ethanol extracts of naturally grown and cultivated Satureja macrantha samples were reported. The essential oil, flavour and terpenoid-steroid-flavonoid contents of S. macrantha samples were determined by GC-MS and their phenolic contents by LC-MS/MS. Besides, the biological activities of the samples were investigated for their antioxidant, anti-Alzheimer, antimicrobial, cytotoxic, antityrosinase, antiurease, antielastase and anticollagenase properties. The phenolic content and antioxidant capacity of the cultivated sample were higher than those of the naturally grown sample. According to the GC-MS results, terpinene-4-ol (30.9%) and p-cymene (56.7%) were determined as the major components in the essential oils of the naturally grown and cultivated S. macrantha, respectively. The flavour analysis results showed that cis-sabinene hydrate (20.7%) and carvacrol (42.2%) were found to be the major components in the naturally grown and cultivated samples, respectively. While the naturally grown sample was rich in abietane diterpenoids (ferruginol (17.5 mg analyte/g extract) and sugiol (4.2 mg analyte/g extract)), these components were not detected in the cultivated sample. The rosmarinic acid content (0.20 and 24.87 mg analyte/g extract, respectively) of the cultivated sample was found to be significantly higher than that of the natural sample. The biological activities of the samples were determined to be changed in parallel with their chemical contents that are due to factors such as climatic conditions, and soil structure.
Chemical Profile by Lc-ms/Ms, Gc/Ms and Antioxidant Activities of the Essential Oils and Crude Extracts of Two Euphorbia Species
(Taylor & Francis Ltd, 2015) Ertas, Abdulselam; Yilmaz, Mustafa Abdullah; Firat, Mehmet
In this study, it was aimed to investigate the chemical composition and antioxidant activities of two Euphorbia species. The major component of the fatty acid compositions obtained from the petroleum ether extracts was identified as palmitic acid for Euphorbia gaillardotii and Euphorbia macroclada. The main constituents of the essential oils were identified as arachidic acid for E. gaillardotii and tetratetracontane for E. macroclada. Among the 27 studied compounds, hesperidin, rutin, hyperoside and quinic, malic, gallic and tannic acids were found to be the most abundant compounds in the two Euphorbia species. The methanol extracts of E. gaillardotii and E. macroclada showed strong antioxidant activity in all tested methods. Particularly, IC50 values of E. macroclada methanol extract that was the richest in terms of total phenolic-flavonoid contents were found to be lower than alpha-tocopherol and butylated hydroxytoluene in beta-carotene bleaching, 2,2-diphenyl-1-picrylhydrazyl free and ABTS cation radical scavenging methods.
Cirsium Semzinanicum (Asteraceae), a New Species From Hakkari, Turkey
(Pensoft Publishers, 2016) Firat, Mehmet
Cirsium semzinanicum sp. nov. (Asteraceae) is described as a new species from Hakkari, Turkey. The new species is a part of the sect. Epitrachys (Cardueae) and similar to Cirsium karduchorum, from which it differs in morphological characters such as leaves, involucre, phyllaries, corolla, achens and pollen morphology. Geographical distribution, habitat and IUCN conservation status of this species are given.
Clinopodium Hakkaricum (Lamiaceae), a New Species From Turkey
(Finnish Zoological Botanical Publishing Board, 2009) Firat, Mehmet; Dirmenci, Tuncay
Clinopodium hakkaricum Dirmenci & Firat (Lamiaceae) sp. nova from Hakkari province, SE Anatolia, Turkey is described and illustrated. It clearly differs from the other Clinopodium taxa in Turkey and adjacent areas. Affinities to and differences from C. insulare, C. menthifolium, C. molle and C. nepeta are elaborated. These related species are revised for Turkey and adjacent areas and an identification key is provided. The geographic localities of the examined specimens are presented in a distribution map.
Clinopodium Serpyllifolium Subsp Sirnakense (Lamiaceae), a New Taxon From South-Eastern Anatolia, Turkey
(Magnolia Press, 2015) Firat, Mehmet; Akcicek, Ekrem; Kaya, Ayla
Clinopodium serpyllifolium subsp. sirnakense (Lamiaceae) is described as a new subspecies from south-eastern Anatolia, Turkey. It is considered morphologically similar to Clinopodium serpyllifolium subsp. brachycalyx, from which it differs in indumentum, calyx characters and mericarp surface. Detailed illustrations are provided along with a distribution map, an IUCN ranking and notes on taxonomic affinities.
Comparison of Chemical and Biological Properties of in Vivo and in Vitro Samples of Salvia Siirtica Kahraman, Celep & Dogan Extracts Prepared With Different Solvents
(Elsevier, 2021) Fidan, Hilal Saruhan; Kilinc, Fatih Mehmet; Yilmaz, Mustafa Abdullah; Akdeniz, Mehmet; Yener, Ismail; Firat, Mehmet; Ertas, Abdulselam
The use of Salvia species among the public and their importance in the scientific world increase due to their numerous pharmacological and biological activities on a daily basis. In this study, the phytochemical contents of different parts (root, branch, leaf, flower, whole) of different specimens of Salvia siirtica (SS) (in vivo and in vitro) extracts prepared with various solvents (petroleumether, chloroformand ethanol) were determined using different techniques (GC-MS and LC-MS/MS) and the results were compared. In addition, biological activities (antioxidant, cytotoxic, anticholinesterase antiurease, antityrosinase, antielastase and anticollagenase) of all samples were determined and compared. The antioxidant potential of the analysed samples was found to be high, and their enzyme activity potential was low. Besides, in vitro SS-TIS (Temporary Immersion System) sample showed high cytotoxic activity (viability% 2.12 +/- 0.06) against MCF-7 (breast cancer) cell line. The results of GC-MS and LC-MS/MS analyses indicated that ferruginol and sugiol could be isolated from the ethanol extract of S. siirtica roots, salvigenin and beta-sitosterol from the chloroform extract of the aerial parts, and phenolic compounds from the ethanol extract of the aerial parts. In addition, the amount of rosmarinic acid, caffeic acid and 12-demethylmulticauline of in vitro samples were found to be higher than those of in vivo samples. Furthermore, all samples, both in vivo and in vitro, contained high levels of rosmarinic acid and beta-sitosterol. The whole chloroform extract (SSWC) could be the source extract for salvigenin (33952.13 mu g/g) and beta-sitosterol (16369.71 mu g/g), and the root ethanol extract (SSRE) for ferruginol (17721.99 mu g/g). As a result, it is understood that the nature of the plant material, the choice of an appropriate solvent and the parts of the material used are quite effective in chemical content. S. siirtica promise potential natural antioxidant agent in food and/or pharmaceutical industry due to its phytochemical content (rosmarinic and caffeic acids, ferruginol, salvigenin) and antioxidant activity. (C) 2021 Published by Elsevier B.V. on behalf of SAAB.
Comprehensive Study of All Gundelia L. Taxa Exists in the Globe: an Insight on Lc-ms/Ms Based Phytochemical Investigation and Bioactivity Potential of 22 Species
(Pergamon-elsevier Science Ltd, 2023) Tarhan, Abbas; Firat, Mehmet; Topal, Giray
Species from the Gundelia genus are among the important main therapeutic herbs widely used in Middle Eastern nations' traditional medicine. The emphasis of the current investigation was on the bioactive phytochemicals and biopharmaceutical efficacy of all 22 species of Gundelia taxa worldwide. Herewith, ethanolic extracts of root, stem-leaf and flower parts of twenty two Gundelia species were prepared and their bioactive phytochemicals were quantified by a comprehensive and validated LC-MS/MS (liquid chromatography tandem mass spectrometry) method. The results of the LC-MS/MS study revealed that, chlorogenic acid (0.042-93.52 mg/g extract), quinic acid (3.860-63.761 mg/g extract), kaempferol-3-O-glucoside (0.019-42.737 mg/g extract), luteolin-7-O-glucoside (cymaroside) (0.013-34.017 mg/g extract), quercetin-3-O-glucoside (0.021-24.241 mg/g extract), apigenin-7-O-glucoside (0.006-15.859 mg/g extract), 1,5-dicaffeoylquinic acid (0.014-4.827 mg/g extract) and caffeic acid (0.020-4.082 mg/g extract) amounts were remarkable in the overall assessment of the quantification results. Furthermore, bioactivities of the studied extracts were determined by evaluating total phenolic-flavonoid contents and ABTS (cation radical scavenging activity), DPPH (free radical scavenging activity) and CUPRAC (cupric reducing antioxidant capacity) antioxidant test assays. With a few exceptions, all antioxidant test assays showed that the root ethanol extracts of the examined Gundelia species had stronger antioxidant activity than their flower and stem-leaf extract counterparts. Based on the results of the ABTS assay, it was discovered that the antioxidant capacities of root-ethanol extracts of G. purpurascens, G. dersim, G. armeniaca, G. armata, G. aragatsi, G. microcephala, G. tournefortii and G. tehranica (IC50 < 10 & mu;g/mL) were at least as high as those of BHT (Butylated hydroxytoluene) (IC50: 11.35 & PLUSMN; 0.22 & mu;g/mL) and & alpha;-tocopherol (IC50: 9.23 & PLUSMN; 0.12 & mu;g/mL), which were used as the reference compounds. To conclude, this is the first study in which the phytochemical contents and antioxidant activities of different parts of all Gundelia taxa in the world were determined together. Moreover, in this study, the phytochemical quantitation and bioactivity of G. microcephala, G. cappadocica, G. tehranica, G. aragatsi, and G. tenuisecta species were evaluated for the first time.
Comprehensive Study of Chemical Composition and Biological Activity of Thymus Pubescens Boiss. Et Kotschy Ex Celak
(Elsevier, 2022) Yigitkan, Serkan; Akdeniz, Mehmet; Yener, Ismail; Seker, Zeki; Yilmaz, Mustafa Abdullah; Firat, Mehmet; Orhan, Ilkay Erdogan
The use of Thymus species amongst the public and their importance in the scientific world is increasing day by day. In addition to being consumed as tea and spice, Thymus species are used as folk medicine for respiratory, digestive, skin, circulatory, genital, nervous, visual and urinary diseases. In this study, it was aimed to examine the essential oil and ethanol extract of the root and aerial parts of Thymus pubescens Boiss. et Kotschy ex Celak in the terms of their biological activity and chemical content. The phenolic content of the species was determined by LC-MS/MS, while triterpenoid content, the chemical composition of the essential oil as well as flavour was determined by GC-MS. In addition, their antioxidant and cytotoxic activities, as well as acetyl- (AChE) and butyrylcholinesterase (BChE), urease, tyrosinase, elastase, collagenase, HMG-CoA reductase and angiotensin-converting enzyme (ACE) inhibitory activities were studied. Thymol (53.33%) was determined as the major component in the essential oil, while b-fenchyl alcohol (43.87%) was determined as the major component of the flavour. According to the LC-MS/MS and GC-MS results, it was found that especially the aerial parts of the species have a high amount of rosmarinic acid (3875.76 mg analyte/g extract), quinic acid (2392.55), naringenin (970.39), oleanolic (92785.96) and ursolic (63373.32) acids. The essential oil of T. pubescens species was observed to show high activity in four antioxidant assays, e.g. inhibition of lipid peroxidation, DPPH and ABTS radical scavenging activity as well as CUPRAC, while the ethanol extracts showed moderate antioxidant activity. In enzyme inhibition assays, the aerial parts exerted marked BChE, elastase, and collagenase inhibitory activities (92.43 +/- 1.28%, 42.59 +/- 0.56, and 48.61 +/- 0.39 at 100 mg/mL, respectively). On the other hand, AChE, urease, tyrosinase, HMG-CoA reductase and ACE inhibitory activities of all extracts were from low to moderate levels. In particular, the aerial parts of the species displayed a high cytotoxic effect (vitality%: 6.82 +/- 0.01 at 200 mg/mL) in breast cancer (MCF-7) cell line. Due to its remarkable antioxidant capacity, high content of rosmarinic, oleanolic, and ursolic acids and especially BChE, elastase, and collagenase inhibitory activity, T. pubescens has the potential for using in food supplements, food preservatives, cosmetics, and pharmaceutical industries.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.
A Comprehensive Study on Chemical and Biological Investigation of Thymus Brachychilus Jalas: A Rich Source of Ursolic and Oleanolic Acids
(Taylor & Francis inc, 2024) Akdeniz, Mehmet; Yigitkan, Serkan; Yilmaz, Mustafa Abdullah; Yener, Ismail; Varhan Oral, Elif; Firat, Mehmet; Ertas, Abdulselam
The significance of Thymus species in the scientific community is growing steadily due to their extensive utilization in traditional medicine, food industry, and pharmaceutical sector, owing to their abundance in essential oil and phytochemical content, rendering them commercially significant species. The current work focuses on conducting a comprehensive analysis of the ethanol extract and essential oil derived from the root and aerial portions of Thymus brachychilus Jalas, an endemic species that has not been previously investigated. Additionally, a novel GC-MS (gas chromatography-mass spectrometry) technique was developed to quantify the levels of triterpenoids, which are frequently found in many plants, particularly those belonging to the Lamiaceae family. The approach was then used to assess the triterpenoid content of the species. While the phenolic content of the species was determined by LC-MS/MS (liquid chromatography-tandem mass spectrometry), the chemical composition of triterpenoid, essential oil and flavor (aroma) of the plant was determined by GC-MS. Eucalyptol was the primary ingredient in both the essential oil and the flavor, accounting for 11.05% and 12.35%, respectively. In the DPPH and ABTS radical scavenging techniques, the root ethanol extract exhibited the greatest antioxidant activity with IC50 values of 26.70 +/- 0.23 and 19.16 +/- 0.11 mu g/mL, respectively. There was a high level of urease (59.54 +/- 1.67% at 100 mu g/mL) inhibitory activity observed in the root ethanol extract in enzyme inhibition assays, as well as angiotensin (94.80 +/- 0.56%), elastase (40.19 +/- 0.39%), and collagenase (48.26 +/- 0.12%) inhibition in the aerial ethanol extract. Moreover, the MCF-7 cell line for breast cancer had a strong cytotoxic impact when exposed to the essential oil of the species (vitality%: 1.45 +/- 0.01 at 200 mu g/mL. The LC-MS/MS and GC-MS studies revealed that the roots had a significant concentration of rosmarinic acid (15,801 mu g analyte/g extract), but the aerial portions had a substantial quantity of ursolic acid (152,374 mu g analyte/g extract). The plant exhibits potential in the food, cosmetic, and pharmaceutical sectors on account of its elevated levels of rosmarinic and ursolic acid, superior antioxidant capacity, and angiotensin and urease inhibitory effect.
Content Analysis, Nanoparticle Forming Potential and Acaricidal Effects on Tick (Hyalomma Marginatum) of Essential Oils From Two Common Thymbra Species
(Taylor & Francis Ltd, 2024) Kaya, Eda Cavus; Akdeniz, Mehmet; Ugurlu, Pelin; Yener, Ismail; Firat, Mehmet; Ertas, Abdulselam
In this study, the fatty acid, essential oil and aroma composition of Thymbra sintenisii Bornm. & Aznav. subsp. sintenisii Bornm. & Aznav (TSS) and Thymbra sintenisii Bornm. & Azn. subsp. isaurica P.H.Davis (TSI) were determined by GC-MS. The cytotoxic activity was determined with MTT assay and antioxidant activities were determined with DPPH, ABTS, CUPRAC methods. Urease, acetyl, butyryl, angiotensin, alpha-amylase, tyrosinase, elastase, and collagenase enzyme inhibition activities of these species were also determined. The acaricidal effects of essential oils from aerial parts of TSS and TSI on ticks and the nanoparticle formation potential of their essential oils with AgNO3 were determined and compared. The main components of TSS essential oil and aroma were determined as carvacrol and o-cemen (66.78 and 35.44%, respectively), the major components of TSI essential oil and aroma were carvacrol and gamma-terpinene (63.78 and 31.44%, respectively). The species exhibited remarkably high antioxidant activity, particularly in the ABTS and CUPRAC methods. It was also determined that the butyrylcholinesterase and acetylcholinesterase enzyme inhibition activities (Inhibition%: 76.12 +/- 0.83 and 74.13 +/- 0.33, respectively) of the species were quite high. Moderate antimicrobial activity against the bacteria was observed for both essential oils, with MIC values ranging from 312 to 1250 mu g/mL. Moreover, essential oils of these species showed good acaricidal activity on Hyalomma marginatum (LC50: 11.82 mu g/mL for TSS; LC50: 57.64 mu g/mL for TSI). The high antioxidant, anticholinesterase, and acaracidal activities observed in both species are attributed to the presence of carvacrol in their essential oils. The species have potential to be used in food supplements-preservatives, pharmaceutical industry.