Browsing by Author "Furan, Mehmet Alp"

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Bazı Türk Yazlık Ekmeklik Buğday Çeşitleri Arasındaki Genetik Farklılığın Ssr Markörleriyle Belirlenmesi
(2017) Geboloğlu, Merve Dilek; Furan, Mehmet Alp
Tahıllar içerisinde buğday, ilk çağlardan beri kültürü yapılan ve stratejik önemi yüksek olan bir besin kaynağıdır. Nüfusun artmasıyla birlikte birim alandan alınan verimi arttırma ihtiyacı doğmuştur. Buğdayda birim alandan alınan verimi arttırmak çeşitli ıslah yöntemleri ile mümkün olabilir. Gen kaynaklarının genetik benzerlik-farklılıklarının bilinmesi bitki ıslahı açısından oldukça önemlidir. Son yıllarda buğdayda yapılan çalışmalarda moleküler markör yöntemleri sıklıkla tercih edilmektedir. Bu çalışmada, Türkiye'nin farklı karakterizasyonunun belirlenmesi amaçlanmıştır. Bu amaçla 32 adet SSR primeri kullanılarak spesifik DNA bölgelerinin çoğaltılması sağlanmış ve kapillar yöntemle elektroforez işlemini gerçekleştirilmiştir. Yapılan moleküler markör analizi sonucunda çeşitler arasındaki genetik benzerlik ve farklılıklar moleküler düzeyde ortaya konmuştur. Ayrıca bu çalışmayla moleküler markör kullanımının ıslah çalışmaları için önemi ve gerekliliği bir kez daha vurgulanmıştır.
Chemical and Genetic Variability of Selected Turkish Oregano (Origanum Onites L.) Clones
(Springer Wien, 2010) Tonk, Fatma Aykut; Yuce, Sueer; Bayram, Emine; Giachino, R. Refika Akcali; Sonmez, Cigdem; Telci, Isa; Furan, Mehmet Alp
Turkish oregano (Origanum onites L.) is a major aromatic plant that belongs to Labiatae family. In this work fourteen Turkish oregano clones have been characterized chemically and genetically. Essential oil obtained by hydro-distillation was characterized by gas chromatography. Genetic variation was determined by use of random amplified polymorphic DNA (RAPD) markers. Variation of essential oils in the selected clones was subjected to cluster analysis, and two chemotypes, carvacrol and thymol, were identified. Carvacrol was found to be the main component in all clones except clone-661, the main component of which was thymol. The oregano clones were divided into three main groups by clustering on the basis of RAPD markers. Genetic similarity values among the oregano clones ranged between 0.49 and 0.73 which was indicative of a low level of genetic variation. Clones originating from locations close to each other had similar RAPD markers. Correlation analysis of the genetic distance matrix and the Euclidian distance matrix revealed no significant correlation between them. The results also indicated that there is no relationship between genetic structure of the selected clones and essential oil composition.
Comparative Assessment of Four Different Markers (Ssr, Issr, Srap and Ipbs) in Genetic Diversity Studies
(Parlar Scientific Publications (p S P), 2018) Furan, Mehmet Alp
There are many molecular markers used by researchers to assess genetic relationships among species or intra-species in molecular studies. They use most of them to determine the precise similarities or dissimilarities. When researchers determine which to use, they consider a number of criteria, such as infrastructure, finance, labor and purpose. In this study, genetic diversity has been observed with using four different marker systems such as: Simple Sequence Repeat (SSR), Inter Simple Sequence Repeat (ISSR), inter primer-binding site (iPBS) and sequence related amplified polymorphism (SRAP) and all binary data set from these marker systems were calculated with the objective of assessing the marker system efficiency. The highest polymorphism percentages respectively were obtained from SSR marker with 96.6% and SRAP marker with 95% and the Cophenetic correlation coefficient respectively was estimated for each marker system r=0.85; 0.84; 0.98; 0.97. In a combined analysis of these markers, genetic similarities among all individuals ranged from 0.51 to 0.84, with a mean similarity of 0.64. However, correlations between the different molecular marker systems have shown that complementary information produced by different markers (codominant and dominant) for genetic studies involving polymorphism, and that the prediction of relationships is important.
Comparison of Some Yield and Quality Characteristics of Hulled and Hull-Less Barley Cultivars in Southeastern Anatolia Region Conditions
(2022) Ateş, Eren; Furan, Mehmet Alp
Yıllık bir bitki olan kavuzlu ve kavuzsuz arpanın kullanımı Türkiye ve diğer ülkelerde benzerlik göstermektedir. Kavuzsuz arpalar genelde insan gıdası temininde kullanılırken, kavuzlu arpa hayvan yemi olarak kullanılmaktadır. Kavuzlu ve kavuzsuz arpa çeşitleri besin içeriği, uygulama bölgeleri, bulunabilirlik ve fiziksel özellikler açısından farklılık göstermektedir. Küresel ısınma, nüfus artışı vb. çevresel kaygıların bir sonucu olarak gıda talebi son yıllarda istikrarlı bir şekilde artmaktadır. Gerçekten de, arpanın gıda arzındaki önemi göz ardı edilemez. Bu araştırmada materyal olarak 6 kavuzlu arpa ve 2 kavuzsuz arpa çeşidi kullanılmıştır. Saksı denemesi olarak açık sera koşularında yetiştirilen arpa bitkilerinde verim ve verim unsurları ile kalite analizleri yürütülmüştür. Denemede bitki boyu, başak uzunluğu, başakta tane sayısı, başakta tane ağırlığı, bitki tane verimi, bin tane ağırlığı, saksı tane verimi gibi tarımsal karakterler ve protein oranı, selüloz oranı gibi kalite karakterleri incelenmiştir. 2018- 2019 yılı iklim koşullarında Diyarbakırda Yapılan çalışmada incelenen parametrelerdeki ölçümler sonucunda ortalamalara göre kavuzlu arpada bitki tane verimi ve başaktaki tane sayısının kavuzsuz arpaya oranla belirgin olarak daha yüksek olduğu gözlenmiştir. Ancak protein oranları bakımından kavuzsuz arpaların protein oranının kavuzlu arpaya oranla daha fazla olduğu gözlenmiştir.
Determination of Variety Mixtures in Local Bread Wheat (triticum Aestivum L.) Varieties Grown in the Erciş District of Van
(2022) Kapan, Duygu; Furan, Mehmet Alp
Bu araştırma; 2022 yılında Van'ın Erciş ilçesinde 11 farklı köyde yerel ekmeklik buğday yetiştiriciliği yapılan 12 farklı tarlada yürütülmüştür. Belirlenen tarlalardan alınan örneklerle, o tarlada yetiştirilen yerel ekmeklik buğday çeşitlerindeki çeşit karışımını tespit etmek ve karışımda yer alan yerel ekmeklik buğday çeşitlerini verim unsurları bakımından karşılaştırmak amaçlanmıştır. Elde edilen veriler tesadüf blokları deneme desenine göre analiz edilmiştir. Burada blok olarak lokasyonlar alınmış ve bloklar arasındaki farklılıklar lokasyonlar arasındaki farklılıklar olarak değerlendirilmiştir. Örnekleme yapılan tarlalarda yerel ekmeklik buğday çeşitlerinden sadece Karakılçık, Kırmızı Tir ve Beyaz Tir çeşitleri ve bazı tarlalarda önemli oranda yabani çavdar tespit edilmiştir. Tüm lokasyonlarda Karakılçık'ın baskın olduğu (% 59.79 - 94.75) görülmüş olup, bazı yerlerde tarladaki çeşit ve tür karışımı göreli olarak daha yüksek çıkmıştır. Lokasyonlardan alınan örneklerde bitki boyu, başak boyu, başakta başakçık sayısı, steril başakçık sayısı, başakta tane sayısı ve bin tane ağırlığı bakımından ölçümler yapılmıştır. Çalışmada incelenen verim unsurlarından bitki boyu, başak boyu, başakta başakçık sayısı ve başakta tane sayısı bakımından en yüksek değerler karakılçık çeşidinden (Sırasıyla 84 cm, 10.2 cm, 17.0 adet ve 28.8 adet) elde edilirken bin tane ağırlığı bakımından en yüksek değer 43.7 g ile Kırmızı Tir çeşidinden elde edilmiştir. Başakta steril başakçık sayısı ve başak tane verimi bakımından ise çeşitlere ait ortalamalar arasında bir fark bulunmamıştır. Örneklemelerin yapıldığı lokasyonlarda sadece bitki boyu ve başakta tane sayısına ait ortalamalar arasında farklılıklar önemli bulunurken diğer verim unsurlarına ait ortalamalar arasında bir farklılık tespit edilmemiştir.
Effects of Arbuscular Mycorrhizal Fungi (Amf) and Biochar on the Growth of Pepper (Capsicum Annum L.) Under Salt Stress
(Springer, 2023) Gunes, Hasret; Demir, Semra; Erdinc, Ceknas; Furan, Mehmet Alp
Soil salinity is a significant abiotic factor that negatively affects the growth and yield of many plants. Separately, biochar and arbuscular mycorrhizal fungus contributed positively to salt-stressed plant growth. To alleviate salinity stress and promote pepper growth, biochar and mycorrhiza are rarely researched together. In this work, the effects of salt stress (AMF) and biochar (BC) applications on the pepper (Capsicum annum L.) plant's morphological and physiological growth characteristics and some soil properties were investigated. Biochar (2% and 4%) and AMF inoculum (Funneliformis mosseae (Fm) and ERS commercial AMF inoculum) were applied to a commercial pepper cultivar (Sera Demre 8) at varying salt concentrations (0 mM, 50 mM, 100 mM, and 150 mM) in order to determine the optimal treatments (AMF inoculum and biochar concentration). In general, when the salt concentration increased, the morphological growth values of the pepper plant and the AMF density decreased, but the EC value of the soil increased. In general, the plants were able to endure a salt concentration of 100 mM, but their sensitivity increased when exposed to 150 mM NaCl. Biochar and AMF independently increased plant growth parameters, leaf relative water content (LRWC), and phosphorus (P), while decreasing membrane injury index (MII) values. The interaction biochar and AMF positively improved microbial activity under conditions of salt stress, while mycorrhizal dependency did not arise in AMF treatments. Based on the results, it was found that the 2% biochar ratio and F. mosseae were beneficial to the morphological development and growth of AMF. Therefore, it was determined that the synergistic effect of AMF and biochar might be used as a viable and sustainable agricultural alternative to prevent salt stress damage in pepper growing.
Effects of Arbuscular Mycorrhizal Fungi (Amf) and Biochar on the Growth of Pepper (Capsicum Annuum L.) Under Salt Stress (Jun, 10.1007/S10343-023-00897-2, 2023)
(Springer, 2023) Gunes, Hasret; Demir, Semra; Erdinc, Ceknas; Furan, Mehmet Alp
Exploring the Complete Chloroplast Genomes of Key Lamiaceae Species Uncovers the Secrets of Evolutionary Dynamics and Phylogenetic Relationships
(Springer, 2025) Furan, Mehmet Alp; Yildiz, Faruk; Kaya, Ozkan
Chloroplast genome analysis provides crucial insights into plant evolution, classification, and conservation strategies. This study aimed to conduct a comprehensive comparative analysis of chloroplast genome architecture, gene content, and evolutionary relationships among five species of Lamiaceae (Lavandula angustifolia, Mentha x piperita, Ocimum x africanum, Salvia japonica, and Thymus serpyllum). Complete chloroplast genome sequences were retrieved from the NCBI database and analyzed using a systematic bioinformatics pipeline. Genome annotation was performed using Geneious Prime software, while repetitive sequence analysis was conducted using Tandem Repeats Finder. Phylogenetic relationships were reconstructed using MEGA software, implementing both the DualBrothers model and Neighbor-Joining method. The analyzed genomes exhibited the characteristic quadripartite structure, with sizes ranging from 152,048 to 153,995 base pairs and GC content between 37.8 and 38.0%. Each genome contained 131-134 genes, including 50 protein-coding sequences, 8 rRNA genes, and 37 tRNA genes. Comparative analysis revealed region-specific GC content variations, with IR regions showing the highest (43.0-43.4%), followed by LSC (35.9-36.2%) and SSC regions (31.6-32.1%). Codon usage analysis demonstrated a significant bias toward T/C-ending codons, particularly TTT, AAA, and AAT, correlating with the high AT content. Notable variations were observed at the LSC/IR/SSC junction regions, attributed to IR expansion and contraction. Molecular clock analyses indicated consistent evolutionary rates across the studied species. These findings provide valuable insights into the molecular evolution of Lamiaceae chloroplast genomes and establish a foundation for future research in plant molecular biology, systematic studies, and conservation efforts.
Peroxidase Gene Markers Revealed Genetic Diversity and Population Structure in Okra Germplasm
(Springer, 2022) Yildiz, Mehtap; Furan, Mehmet Alp; Kocak, Metin; Sadik, Gokhan; Kuzgun, Cansu; Al-Khafaji, Mahdi; Bahjat, Noor Maiwan
Okra is a widely grown vegetable crop in various regions of the world and provides significant amounts of vitamins and minerals. Numerous researchers have documented the efficacy of phenotypic and molecular classification of the diverse okra germplasm collections found throughout the world. The genetic variation of Turkish okra germplasm, which included 39 genotypes, was characterized using 14 POGP (peroxidase gene polymorphism) primer pairs in comparison to other world okra genotypes and accessions, which contained 27 genotypes. A total of 84 bands were generated using 14 POGP primer pairs, with 43 (51%) of them being polymorphic between okra accessions. The values of polymorphism information content ranged from 0.03 to 0.99, with an average of 0.48. The range and mean values for gene diversity (h) were 0.03-0.42 and 0.25, respectively. Shannon's information index (I) varied between 0.07 and 0.62 for each POGP marker, with a mean of 0.40. The most divergent genotypes were found to have a GS value of 0.23, with IN-7 and TR20-3 being the genetically most distant. The STRUCTURE, PCoA and Neighbor-joining analysis separated the okra germplasm into three populations. AMOVA revealed 23 and 77% variance across and within populations, respectively. Although the germplasm of okra is generally homogenous, the influence of shared genes is more significant. For the first time, the genetic composition of okra was determined using peroxidase gene markers.
Rapd and Essential Oil Characterization of Turkish Basil (Ocimum Basilicum L.)
(Springer Wien, 2014) Giachino, R. Refika Akcali; Sonmez, Cigdem; Tonk, Fatma Aykut; Bayram, Emine; Yuce, Suer; Telci, Isa; Furan, Mehmet Alp
Sweet basil (Ocimum basilicum L., Lamiaceae), an important medicinal plant and culinary herb due to its delicate aroma and fragrance, shows great variation in both morphology and essential oil components. Genetic variation among basil accessions in Turkey has not been extensively examined with molecular markers. Genetic diversity was determined using random amplified polymorphic DNA (RAPD) markers of 14 genotypes of basil. A total of 375 bands were obtained from the RAPD analysis, and 273 of them (70.3 %) were polymorphic. The RAPD analysis allowed the grouping of samples into two main clusters. Genetic similarity values among the basil genotypes ranged between 0.46 and 0.87. Considerable genetic diversity was determined among basil genotypes. Essential oils were obtained by hydro-distillation and were characterized by gas chromatography. A total of 17 chemical components were identified. The evaluated genotypes of O. basilicum can be classified into seven chemotypes: (1) Linalool (7, 12, 16, 22, 25A and 33), (2) Methyl chavicol (6, 10A), (3) Citral/methyl chavicol (10L, 17), (4) Methyl eugenol (11), (5) Methyl cinnamate/linalool (23), (6) Linalool/methyl eugenol (25K), and (7) Methyl chavicol/linalool (Let). The chemical variability obtained from the essential oil composition of the genotypes in the study was remarkable. The chemical characterization of genotypes 10L and 17 was rich in citral (42.17 and 44.80 %) and methyl chavicol (30.56 and 32.03 %). Citral/methyl chavicol can be assessed as a new chemotype of basil cultivated in Turkey. The basil genotypes were grouped into two major clusters for both the RAPD analysis and chemical characterization with very few exceptions (genotype n. 6). A correlation analysis of the genetic distance matrix and the Euclidian distance matrix showed relatively low values (r = -0.40). The results demonstrated a certain degree of correspondence between chemical and molecular data.