Browsing by Author "Gecit, Ilhan"

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Application of Ureteroscopic Pneumatic Lithortripsy in Patients With Ureter Calculi: Our Five Years Experience
(Professional Medical Publications, 2012) Gecit, Ilhan; Pirincci, Necip; Taken, Kerem; Benli, Erdal; Ceylan, Kadir
Objectives: To evaluate the efficacy and reliability of ureteroscopic pneumatic lithoripsy (URS-PL) in patients with ureter calculi. Methodology: A total of 612 patients who were treated with URS-PL in Medical school of Yuzuncu Yil University, Van State Hospital and Bingol State Hospital in the period between April 2005-May 2010 were studied. Diagnoses of ureter stones were made with intravenous pyelography (IVP) or computerize tomography (CT). Ureteral balloon dilation was not performed on any patient. On the post-operative 1st, 7th and 21st days, control direct urinary system graphic (DUSG) was taken. The rates of stone free and complication were analyzed with the ki-kare test. P < 0.05 was accepted as significant. Results: The mean age of the patients was 39 +/- 10 (18-75). There were 417 males (68.13%) and 195 females (31.86%). The mean volume of calculi in patients was 10.1 +/- 2.3 mm. Of these patients, 121 had proximal ureter stone, 169 medial ureter stones and 322 had distal ureter stones. In the first session of URS-PL, the calculi of 96 patients in proximal ureter (80%), those of 143 patients in medial ureter (85%) and those of 305 patients (95%) in distal ureter were cracked successfully. The mean stone free rate was 92.4% (79.4% in upper ureter, 94.1% in middle ureter and 93.7% in distal ureter (P < 0.05). Overall complication rate was 7.6% (7.7% in distal ureter, 3.9% in middle ureter and 11.8% in proximal ureter) (P>0.05). Most common peroperative complications of the procedure were ureteral perforation 4,2% (n=26), infection 2.2% (n=14), and mucosal laceration 1.9% (n=12). The most common late complication was ureteral stricture in 1.3% (n=8) of patients. Conclusion: Although URS-PL is a commonly used application for the treatment of patients with proximal and middle ureter stones, its common usage could also be extended to patients with distal ureter stones in the first stage of treatment, and it could be considered as an efficient and reliable procedure.
The Bacteria Isolated From the Urine Cultures of Patients Admitted To Duzce Ataturk State Hospital Urology Outpatient Clinic and Their Antibiotic Resistances
(Duzce Univ, 2013) Gecit, Ilhan; Yuce, Muhterem; Kebeci, Faik; Ozaydin, Cigdem; Oksuz, Sukru; Yavuz, Tevfik
Objectives: The aim of this study was to determine the bacteria and their antibiotic resistance ratios isolated from the urine cultures of patients admitted to our hospital's urology outpatient clinics in order to outline the empirical treatment. Methods: Positive cultures of urine samples were evaluated retrospectively. Cultures of >= 10(5) cfu/ml growth were evaluated. Antibiotic resistance tests were performed according to the Clinical and Laboratory Standards Institute (CLSI) suggestions with Kirby-Bauer disc diffusion method using MuellerHinton agar. Results: In this study, E. coli (69%) was the most common isolate; followed by Klebsiella spp. (9%), S. aureus (9%), Enterococcus spp. (7%), Enterobacter spp. (3%), Proteus spp. (2%) and P. aeruginosa (1%). In E. coli strains, resistance to amoxicillin clavulanic acid (AMC), ciprofloxacin (CIP), and trimethoprim sulfamethoxazole (SXT) were 49%, 45%, 44%; respectively. In S. aureus strains, resistance to oxacillin was 58%. Conclusion: As a result, it is put forward that the treatment of urinary tract infections should be arranged according to the urine culture results because of the high resistance ratios of the frequently used empiric antibiotics.
Beneficial Influence of Topical Extra Virgin Olive Oil Application on an Experimental Model of Penile Fracture in Rats
(Sage Publications inc, 2015) Gunes, Mustafa; Ozkol, Halil; Pirincci, Necip; Gecit, Ilhan; Bilici, Salim; Yildirim, Serkan
Penile fracture (PF) is known as a traumatic rupture of the tunica albuginea of corpus cavernosum. In this study, we aimed to investigate the healing influence of topical extra virgin olive oil (EVOO) on PF through evaluating levels of some oxidative stress biomarkers for the first time. Histopathological evaluation was also realized. A total of 18 male Sprague-Dawley albino rats were divided into three groups of six rats each as control group, in PF (alone) group, and PF + EVOO group. Experimental PF was formed via incising from the proximal dorsal side of the penis in the rats of all groups except control. While in PF (alone) group, fracture was formed and the incision was primarily closed, in PF + EVOO group in addition to foregoing processes, EVOO was also administrated topically twice a day for 3 weeks. At the end of the experiment, all rats were killed and penectomy was carried out. While malondialdehyde, myeloperoxidase, lipid hyroperoxide, and total oxidant status significantly (p < 0.05) increased, reduced glutathione and total free sulfhydryl groups markedly (p < 0.05) decreased in PF (alone) group when compared with PF + EVOO group. Levels of these parameters were reversed to nearly normal values by topical EVOO application. Protection by EVOO is further substantiated via the improved histological findings in PF + EVOO group as against degenerative changes in the rats of PF (alone) group. Our data revealed that EVOO has protective effect in penile cavernosal tissue through probably its antioxidant, free radical defusing, anti-inflammatory, and antimicrobial effects.
Comparative Study in Distal Hypospadias Repair: a Meatal-Based Flaps Technic Vs. Tubularized Incised Plate Urethroplasty
(Bmc, 2024) Kilic, Sinan; Kaba, Mehmet; Gecit, Ilhan
ObjectivesHypospadias is the most common anomaly of the male genital system after undescended testis. More than 200 surgical repair techniques have been described for the treatment of hypospadias due to many different meatus localisations and other accompanying anomalies. Kutlay defined a new technique in 2010 with 10 patients with distal hypospadias patients. We compared this new technique with tubularized incised plate urethroplasty (TIP) which is one of the most frequently performed methods. Methods148 patients with hypospadias who underwent surgery at the Urology and Pediatric Surgery Clinic during the period of January 2010 to December 2022 in the first and 2nd clinic, were retrospectively analyzed. The study focused on the remaining 83 patients with distal hypospadias for the purpose of comparison. ResultsThe mean age of group one was 10.0 (+/- 6.9, range 2-27), while for group two it was 10.8 (+/- 5.9, range 3-23). There was no statistically significant difference between the two groups in terms of anatomical position of the meatus, stricture and fistula complications (p > 0.5). ConclusionThe Kutlay technique has been described as an easy-to-learn and feasible method with reliable results. No significant difference in complications was observed in this study compared with TIP, which is known as the standard technique worldwide. We would like to suggest that the method be considered for further evaluation with larger patient groups and different techniques.
Comparison of Cystoscopy With Diffusion-Weighted Magnetic Resonance Images Used in the Diagnosis and Follow-Up of Patients With Bladder Tumors
(Asian Pacific Organization Cancer Prevention, 2010) Ceylan, Kadir; Taken, Kerem; Gecit, Ilhan; Pirincci, Necip; Gunes, Mustafa; Tanik, Serhat; Karaman, Ihsan
Purpose: To compare diffusion-weighted magnetic resonance imaging (DW-MRI) with cystoscopy in the diagnosis and follow-up of patients with bladder tumor and to investigate any histopathological correlation. Materials and Methods: Totally 59 patients, between 31-85 years (mean age 60 +/- 13) referred to our clinic due to a hematuria complaint were enrolled and evaluated by upper urinary system pathology and then DW-MRI (average 7 days) and cystoscopy. Apparent diffusion coefficients (ADCs) of images were calculated. Results: While a mass in bladder was determined with cystoscopy in 43 out of 59 patients, the mass was not deternined in 16 of the patients(n=34 malign, n=9 benign). While a mass was determined in 40 out of 59 patients with DW-MRI, the mass was not determined in 19 of the patients(n=40 malign, n=19 benign). Regarding ADC values, mean ADC values of 34 patients who were diagnosed with a bladder tumor (1.05 +/- 0.22 x 10(-3)mm(2)/s), were significantly lower than the mean ADC values obtained from the normal bladder wall (1.830 +/- 0.18 x 10(-3)mm(2)/s) whereas a statistically significant difference was found (p<0.001). ADC values in 9 patients with a benign lesion (1,73 +/- 0.12 x 10(-3)mm(2)/s), were not found statistically different from the mean ADC values obtained from the normal bladder wall (1.78 +/- 0.2x10(-3)mm(2)/s) (p>0.05). A significant difference was determined between ADC values of benign lesions and the ADC values of malign lesions (p<0.001). Conclusions: According to cystoscopy, values of DW-MRI's related with sensitivity, specificity and accuracy were found, respectively 90%, 93% and 91%. As the DW-MRI is a non-invasive and a rapid technique, and does not contain ionized radiation and because it is accepted as an important marker of tumor cellularity, it may be used as an alternative in future diagnosis and follow-up of bladder tumors.
Comparison of Dartos Flap and Dartos Flap Plus Spongioplasty To Prevent the Formation of Fistulae in the Snodgrass Technique
(Springer, 2011) Bilici, Salim; Sekmenli, Tamer; Gunes, Mustafa; Gecit, Ilhan; Bakan, Vedat; Isik, Daghan
The aim of our study was to evaluate the role of paraurethral spongial tissue plus dartos flap using an additional urethral cover to prevent fistula formation in patients who underwent surgery with the Snodgrass technique. A retrospective study was performed on 161 patients aged 10 months to 15 years who underwent midpenile and distal hypospadias repair using the Snodgrass technique. The patients were assigned to one of two groups. In Group I (75 patients), the neourethra was covered with the dartos flap, and in Group II (86 patients), the neourethra was covered with the dartos flap plus spongioplasty. Urethral fistulae were encountered in six cases (8%) in Group I, and no fistulae were encountered in Group II. The use of corpus spongiosum as an intermediate layer in urethral coverage, combined with the dartos flap, reduces the likelihood of fistula formation. This procedure can be applied easily and effectively to prevent the formation of fistulae.
Complete Duplication of the Bladder and Urethra in the Coronal Plane: Case Report With Review of the Literature
(Karger, 2013) Pirincci, Necip; Gecit, Ilhan; Gunes, Mustafa; Tanik, Serhat; Ceylan, Kadir
Complete duplication of the bladder and urethra without other congenital anomalies is a very rare congenital anomaly. We report the case of an 8-year-old boy with complete duplication of the bladder and urethra in the coronal plane, without any other associated congenital anomalies and without complaint associated with the urinary tract. Copyright (C) 2012 S. Karger AG, Basel
Conservative Approach in the Treatment of Renal Trauma in Children
(Karger, 2014) Pirincci, Necip; Kaba, Mehmet; Gecit, Ilhan; Gunes, Mustafa; Tanik, Serhat; Ceylan, Kadir
Objective: Our aim was to evaluate the role of a conservative approach in the treatment of renal trauma in the pediatric age group. Materials and Methods: A total of 31 pediatric patients with renal trauma (male = 25, female = 6) in whom we used a conservative approach in our clinic between the years 2002 and 2010 were included in the study. Patients were assessed according to the type of trauma, type of treatment, findings of radiological imaging, concomitant other organ injuries and complications occurring during follow-up. Results: Surgery was required in just 4 of 31 pediatric patients who were treated with the conservative approach. Of the patients who underwent surgery, only one child needed nephrectomy. No complications were detected in the clinical and radiological follow-up of the patients who received the conservative approach. Conclusions: The main objective in the treatment of renal trauma occurring in childhood is to protect the kidneys. If the hemodynannic findings are stable, a conservative approach should be the first preferred method of treatment in every grade of renal trauma that can occur in childhood. Copyright (C) 2013 S. Karger AG, Basel
Dickkopf-1 Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2014) Kaba, Mehmet; Pirincci, Necip; Benli, Erdal; Gecit, Ilhan; Gunes, Mustafa; Yuksel, Mehmet Bilgehan; Kemik, Ahu Sarbay
Background: Evidence indicates that Dickkopf-1 (DKK-1) levels may be a biomarker for cancer risk. The aim of this study was to assess DKK-1 and its correlation with clinic-pathological features in patients with bladder cancer. Materials and Methods: DKK-1 levels were determined in serum samples from 90 patients with bladder cancer before transurethral tumor resection. The concentrations of DKK-1 were determined by using enzyme linked immune-sorbent assay (ELISA). Results: Elevated preoperative DKK-1 levels were associated with tumor stage (p<0.001), grade (p<0.001) and histological grade (p<0.001). Conclusions: The results of our study demonstrated that the level of serum DKK-1 is correlated with both disease progression and increase in the tumor grade. Preoperative serum DKK-1 elevation may thus represent a novel marker for the determination of bladder cancer and the detection of patients with a likely poor clinical outcome.
Effect of Short-Term Treatment With Levosimendan on Oxidative Stress in Renal Tissues of Rats
(Sage Publications inc, 2014) Gecit, Ilhan; Kavak, Servet; Yuksel, Mehmet Bilgehan; Basel, Halil; Bektas, Hava; Gumrukcuoglu, Hasan Ali; Demir, Halit
The aim of this study is to evaluate the influences of short-term treatment with levosimendan (chemical formula: C14H12N6O) on oxidative stress and some trace element levels in renal tissues of healthy rats. A total of 20 male Wistar-albino rats were randomly divided into two groups, each consisting of 10 rats. Animals in the first group were not treated with levosimendan and served as control. Animals in the second group were injected intraperitoneally with 12 mu g/kg levosimendan and served as levosimendan group. Animals in both the groups were killed 3 days after the treatment, and their kidneys were harvested for the determination of tissue oxidant/antioxidant statues and trace element levels in renal tissues. The tissue malondialdehyde level was significantly (p < 0.001) lower in levosimendan group than in controls. The protective enzyme activities such as superoxide dismutase, catalase, and glutathione peroxidase and antioxidant glutathione level were significantly (p < 0.001) higher in levosimendan group than in controls. It was concluded that levosimendan reduced oxidative stress by avoiding lipid peroxidation and production of reactive oxygen species, and overactivating and/or increasing the protective antioxidant enzyme levels in renal tissues of rats. It is supposed that this experimental study provides beneficial data for clinicians in the management of renal tissue damage related to obstruction and/or ischemia.
Effects of Extracorporeal Shock-Wave Lithotripsy Directed at the Parotid Gland on Oxidative Stress Parameters and Some Trace Element Levels in Facial Nerve of Rats
(Wiley, 2012) Kavak, Servet; Garca, M. Fatih; Gecit, Ilhan; Meral, Ismail; Cengiz, Nurettin; Demir, Halit
Introduction: This study was designed to assess the effect of extracorporeal shock-wave lithotripsy (ESWL) exposure of the parotid gland on oxidative stress and some trace element levels in the facial nerves of rats. Methods: Twelve male Wistar albino rats were divided into two groups, each consisting of 6 animals. The rats in the first group served as controls. The left parotid glands of animals in the second group were treated with 1000 18-kV shock waves while anesthetized with ketamine. The animals in both groups were euthanized 72 h after the ESWL treatment, and the right facial nerve was harvested for determination of oxidant/antioxidant status and trace element levels. Results: Lipid peroxidation product malondialdehyde (MDA) and antioxidant glutathione (GSH) levels increased, and the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), decreased in the facial nerves of ESWL-treated rats. The levels of iron, lead, manganese, and cobalt increased, and magnesium, cadmium, and copper levels decreased. Conclusions: ESWL treatment of the parotid gland may increase lipid peroxidation and decrease antioxidant enzyme activity in adjacent tissues such as the facial nerve. It also causes a decrease or increase in many mineral levels of the facial nerve, which is an undesirable condition for normal physiological function. Muscle Nerve, 2012
Effects of Shock Waves on Oxidative Stress and Some Trace Element Levels of Rat Liver and Diaphragm Muscles
(Wiley, 2012) Gecit, Ilhan; Kavak, Servet; Meral, Ismail; Gunes, Mustafa; Pirincci, Necip; Sayir, Fuat; Ceylan, Kadir
This study was designed to investigate whether the short-term extracorporeal shockwave lithotripsy (ESWL) exposure to kidney produces an oxidative stress and a change in some trace element levels in liver and diaphragm muscles of rats. Twelve male Wistar albino rats were divided randomly into two groups, each consisting of six rats. The animals in the first group did not receive any treatment and served as control group. The right-side kidneys of animals in group 2 were treated with two-thousand 18?kV shock waves while anesthetized with 50?mg kg-1 ketamine. The localization of the right kidney was achieved after contrast medium injection through a tail vein under fluoroscopy control. The animals were killed 72 h after the ESWL treatment, and liver and diaphragm muscles were harvested for the determination of tissue oxidative stress and trace element levels. Although the malondialdehyde level increased, superoxide dismutase and glutathione peroxidase enzyme activities decreased in the livers and diaphragm muscles of ESWL-treated rats. Although glutathione level increased in liver, it decreased in diaphragm muscles of ESWL-treated animals. Fe, Mg and Mn levels decreased, and Cu and Pb levels increased in the livers of ESWL-treated animals. Fe and Cu levels increased, and Mg, Pb, Mn and Zn levels decreased in the diaphragm muscles of ESWL-treated animals. It also causes a decrease or increase in many mineral levels in liver and diaphragm muscles, which is an undesirable condition for the normal physiological function of tissues. Copyright (c) 2012 John Wiley & Sons, Ltd.
Effects of Shock Waves on Oxidative Stress in Parotid Gland of Rat
(Sage Publications inc, 2014) Garca, M. Fatih; Kavak, Servet; Gecit, Ilhan; Meral, Ismail; Demir, Halit; Turan, Mahfuz; Cankaya, Hakan
This study was designed to investigate whether extracorporeal shock wave lithotripsy (ESWL) exposure to parotid gland produces an oxidative stress in parotid glands of rats. Twelve male Wistar-albino rats, 6 months of age with an average body weight of 250-300 g, were divided randomly into two groups, each consisting of six rats. The animals in the first group did not receive any treatment and served as control. The left parotid glands of animals in group 2 (ESWL treated) received a thousand 18 kV shock waves after anesthetizing the rats with 50 mg/kg of ketamine. The animals in both groups were killed 72 hours after the ESWL treatment, and the parotid glands were harvested for the determination of lipid peroxidation product malondialdehyde (MDA), antioxidant glutathione (GSH) levels and the activities of antioxidant enzymes such as superoxide dismutase (SOD), GSH-Px and catalase (CAT). It was found that MDA level increased in parotid glands of rats after the ESWL treatment. The SOD, GSH-Px and CAT enzyme activities, and the level of antioxidant GSH decreased in parotid gland of rats after the ESWL treatment. It was concluded that short-term ESWL treatment caused an increase in the free radical production and a decrease in the antioxidant enzyme activity in parotid glands of ESWL-treated rats.
Effects of Shock Waves on Oxidative Stress, Antioxidant Enzyme and Element Levels in Kidney of Rats
(Springernature, 2011) Gecit, Ilhan; Kavak, Servet; Meral, Ismail; Pirincci, Necip; Gunes, Mustafa; Demir, Halit; Ceylan, Kadir
The aim of this study was to investigate the effect of extracorporeal shock wave lithotripsy (ESWL) on kidney oxidative stress and trace element levels of adult rats. Twelve male Wistar albino rats were divided equally into two groups. First group was used as control. The right-side kidneys of animals in second group were treated with 2,000 18-kV shock waves under anesthesia. Localization of the right kidney was achieved following contrast medium injection through a tail vein under flouroscopy control. The animals were sacrificed 72 h after the ESWL treatment, and the kidneys were taken. Malondialdehyde level was higher in the ESWL group than in the control. Reduced glutathione levels, superoxide dismutase, and glutathione peroxidase activities were lower in the ESWL group than those of the control. Fe, Cu, Pb, Mn, Cd, and Ni levels were lower in the ESWL group than in the control, although Mg level was higher in the ESWL group than in the control. In conclusion, the result of the present study indicated that ESWL treatment produced oxidative stress in the kidney and caused impairments on the antioxidant and trace element levels in the kidneys of rats.
Haptoglobin Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2012) Pirinicci, Necip; Gecit, Ilhan; Gunes, Mustafa; Kemik, Ahu Sarbay; Yuksel, Mehmet Bilgehan; Kaba, Mehmet; Aslan, Mehmet
Although alteration in the haptoglobin phenotype has been reported in patients with bladder cancer, serum haptoglobin levels have not been evaluated. We hypothesized that serum haptoglobin can be used as a biomarker. The aim of this study was to evaluate the expression of haptoglobin in bladder cancer and to determine the relationship with clinicopathological features. A total of 68 serum specimens obtained before surgery were used to investigate haptoglobin expression using the sandwich ELISA technique. Serum haptoglobin levels were higher in the patients with bladder cancer compared to healthy controls (p<0.0001). Additionally, the levels of haptoglobin protein increased with increasing tumor grades (p<0.001) and were significantly higher in patients with metastatic disease and the presence of lymphovascular involvement, lymph node metastases and increasing tumor burden (p<0.0001). This study suggests that elevated haptoglobin levels are associated with a higher stage, grade, and extent of distant metastasis and larger tumor size. Haptoglobin may therefore provide a useful diagnostic and treatment biomarker for patients with bladder cancer.
Kutlay Technique for Hypospadias Repair
(Springer, 2012) Gecit, Ilhan; Isik, Daghan; Pirincci, Necip; Bilici, Salim; Gunes, Mustafa; Canbaz, Yasin; Kocak, O. Faruk
Although many techniques have been described, new techniques with a wide range of therapeutic options are needed. The Kutlay technique is a novel technique that is based on the reconstruction of the neourethra with two horizontal meatal-based skin flaps. In the present study, the data of 31 patients who underwent surgery with the Kutlay technique are presented. Thirty-one patients with hypospadias with an average age of 5.6 years who did not have previous hypospadias repair were operated on with the Kutlay technique. Ten patients had chordee. Among those patients, three patients were circumcised. The patients were followed up for 4-13 months (average, 9.3 months). During the follow-up period, a fistula was observed in only one patient. None of the patients developed neourethral dehiscence, meatal stenosis, urethral stricture, wound infection, penile torsion, hematoma, or persistent or recurrent chordee. On uroflowmetry studies, the maximum flow rate of the patients was approximately 10.5 ml/s (range, 6-17 ml/s). The patients were observed to void with a single straight urinary stream in a forward direction. The Kutlay technique is a technique that is easily applied in patients with chordee and that provides a low risk of fistulas and acceptable functional and esthetic results. The factors that reduce the risk of fistula are the lack of the superposition of the suture lines of the skin and the urethra, the reconstruction of the urethra with well-vascularized flaps, and the replacement of the neourethra in its appropriate location through the tunnel created in the glans.
Levels of Serum Trace Elements in Renal Cell Carcinoma Cases
(Asian Pacific Organization Cancer Prevention, 2013) Pirincci, Necip; Gecit, Ilhan; Gunes, Mustafa; Kaba, Mehmet; Tanik, Serhat; Yuksel, Mehmet Bilgehan; Demir, Halit
Trace elements which are essential components of biological structures may also be toxic when present at levels above the amounts required for biological function. In our study, trace element levels were measured with furnace atomic absorption spectrophotometry in 33 newly diagnosed renal cell carcinoma cases (preoperative) and 32 healthy controls. When compared with the control group, it was found that the levels of cadmium (Cd), lead (Pb) were higher and the levels of zinc (Zn), iron (Fe) and manganese (Mn) were lower in the patient group. These changes may be important in the formation of renal cell carcinoma, a question which should be explored with postoperative comparative studies.
Peripheral Mononuclear Leukocyte Dna Damage, Plasma Prolidase Activity, and Oxidative Status in Patients With Benign Prostatic Hyperplasia
(Taylor & Francis Ltd, 2015) Gecit, Ilhan; Meral, Ismail; Aslan, Mehmet; Kocyigit, Abdurrahim; Celik, Hakim; Taskin, Abdullah; Ceylan, Kadir
Objectives: Prolidase plays a major role in collagen turnover, matrix remodeling, and cell growth. Benign prostatic hyperplasia (BPH) may be associated with an increased extracellular matrix deposition. Therefore, the present study was designed to investigate the plasma prolidase activity, oxidative status, and peripheral mononuclear leukocyte DNA damage in patients with BPH. Patients and methods: Twenty-six male patients with BPH and 24 healthy male subjects were included in this study. Blood samples were collected from antecubital vein after an overnight fasting period, and the plasma was separated. Plasma prolidase activity, total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined. The peripheral lymphocyte oxidative DNA damage was determined using an alkaline single cell gel electrophoresis assay (comet assay). Results: The plasma prolidase activity, TOS levels, OSI values, and peripheral mononuclear leukocyte DNA damage were significantly higher (P < 0.001), while the TAC levels were significantly lower (P < 0.001) in patients with BPH than controls. In BPH patients, the prolidase activity was significantly associated with TAC levels (r = -0.366, P < 0.05), TOS levels (r = 0.573, P < 0.001), and OSI (r = 0.618, P < 0.001) and peripheral mononuclear leukocyte DNA damage (r = 0.461, P < 0.001). Conclusions: Our results showed that BPH might be associated with an increased oxidative stress, and also an increased plasma prolidase activity. Increased prolidase activity might play an important role in the etiopathogenesis and/or progression of BPH.
Plasma Human Neutrophil Proteins-1, And-3 Levels in Patients With Bladder Cancer
(Springer, 2013) Gunes, Mustafa; Gecit, Ilhan; Pirincci, Necip; Kemik, Ahu Sarbay; Purisa, Sevim; Ceylan, Kadir; Aslan, Mehmet
Human neutrophil proteins-1, -2, and -3 (HNPs -1, -2, and -3) are expressed in several tumor types. However, the role of HNPs 1-3 in human bladder cancer has not yet been determined. We investigated the association between the plasma levels of HNPs 1-3 and clinicopathological parameters in bladder cancer patients. The plasma levels of HNPs 1-3 were measured in 60 patients with bladder cancer and in 58 healthy controls. The plasma levels of HNPs 1-3 were determined by a solid-phase enzyme-linked immunosorbent assay (ELISA). Plasma samples were obtained before surgery. Plasma samples were permitted to clot and were then stored at -80 A degrees C until use. The levels of the HNPs increased from grade 1 to 4 tumors and this difference was statistically significant (p < 0.001). Additionally, plasma HNP levels were significantly higher in patients with metastatic bladder cancer and in patients with lymphovascular involvement, metastasis of the lymph nodes, and increased tumor burden (p < 0.001). The preoperative plasma levels of HNPs 1-3 paralleled the progression and pathological stages of the malignancies. This study suggests that HNPs 1-3 promote tumor invasion and are potential indicators of disease progression in patients with bladder cancer.
Pregnancy and Renal Cyst Hydatid Disease: Case Report
(Academic Journals, 2012) Gecit, Ilhan; Pirincci, Necip; Taken, Kerem; Eryilmaz, Recep; Ozveren, Huseyin; Deveci, Serkan
Hydatid cyst disease is an important health problem in many countries, including Turkey. While it can impact any organ, it is most often found in the lung and liver. Renal involvement is relatively rare. A diagnosis of renal cystic hydatidosis was established with ultrasound and magnetic resonance in a 22 year old patient who was 25 weeks pregnant after she presented with severe right flank pain. Albendazol was administered at a dose of 10 mg/kg/day. She was closely followed during her pregnancy. At 38 weeks, she gave birth to a healthy baby via vaginal delivery. Following her delivery, the cyst was resected. Post-operatively, she completed an 8 week course of albendazol at a dose of 10 mg/kg/day. 1 year later, the patient has done well with no complications. We presented this case to highlight the lack of consensus in regards to the treatment of renal hydatid cyst disease in pregnancy.