Browsing by Author "Golen, Gokcenur Sanioglu"

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Development of a Simple and Rapid Dna Extraction Method for Aspergillus Flavus
(Sciendo, 2024) Golen, Gokcenur Sanioglu; Akar, Kadir
Aspergillus species are known to be very important in human and domestic animal health. Aspergillus species commonly cause severe systemic and skin infections, as well as allergic lung diseases. With the development of PCR techniques, these methods are used to identify and diagnose fungi. DNA extraction from Aspergillus species is difficult because the fungal cell wall structure is very durable and complex. Fungal DNA extraction methods containing proteinase K and liquid nitrogen are widely used to break down the cell wall. However, these methods cause DNA loss during the extraction in Aspergillus species. In this study, on the contrary, the commonly used DNA extraction by means of ammonium hydroxide, which is generally used to break down chitin in DNA extraction of ticks and plants, is used. The efficiency of the cell wall lysis method from A. flavus with ammonium hydroxide was compared with methods containing proteinase K and liquid nitrogen. For this purpose, DNA extraction of A. flavus was tried using three different methods. As a result, the cell wall of A. flavus was lysed using ammonium hydroxide in this study. The obtained DNA's quality, concentration, and PCR performance were sufficient. This method has been evaluated as a faster, more straightforward, and more economical alternative.
Investigation of the Correlation Between Elisa and Serum Amyloid a in the Diagnosis of Bordetella Bronchiseptica in Dogs
(Wiley, 2025) Akar, Kadir; Golen, Gokcenur Sanioglu; Ekin, Ismail Hakki
BackgroundBordetella bronchiseptica is an essential bacterial pathogen characterized by chronic respiratory disease in dogs known as Kennel cough. The presence of causative antibodies in animals can also be detected by lipopolysaccharide antigen-based enzyme linked immunosorbent assay (ELISA). In recent years, it has been determined that there is a significant relationship between acute phase proteins and diseases, and disease follow-up can be done within the framework of this relationship.MethodsIn this study, blood sera from 150 dogs in an animal shelter in Van province were evaluated for B. bronchiseptica by the homemade ELISA method, and their correlations with serum amyloid A (SAA) were investigated. Blood serum samples were analysed for antibodies against B. bronchiseptica using a homemade ELISA method. Positive animals were also molecularly confirmed using nasal swabs by PCR. A commercial ELISA kit determined SAA levels in blood sera.ResultsEighteen (12%) of the analysed blood serum samples were found positive by the homemade ELISA method. SAA concentrations in the positive blood sera were elevated from 12.7 to <= 38.98 mg/L. SAA concentrations in blood sera serologically positive for B. bronchiseptica were statistically significant.ConclusionsIn this study, in which the relationship between SAA concentration and B. bronchiseptica was investigated for the first time in Turkey, it was concluded that SAA concentration analysis may help diagnose and monitor the disease. In addition, the presence and prevalence of this critical and zoonotic agent causing chronic respiratory tract disease in dogs in Van province was revealed for the first time in this study.
Prevalence of Dermatophytosis in Cats and Dogs in Turkiye: Dominance of Microsporum Canis and First Detection of Trichophyton Rubrum
(BMC, 2025) Golen, Gokcenur Sanioglu; Balevi, Asli; Uslu, Ali; Akar, Kadir; Tasmertek, Melih; Aras, Zeki
Background: Dermatophytosis is a fungal infection that can be zoonotic, with transmission occurring in both directions between humans and companion animals, particularly in settings involving close contact. This study aimed to determine the prevalence and identify the causative agents of dermatophytosis in dogs and cats using conventional and molecular diagnostic methods. A total of 150 animals with dermatological lesions were sampled, including 105 cats and 45 dogs from both household and shelter environments. This cross-sectional study employed direct microscopy and fungal culture as the initial diagnostic methods. PCR targeting the CHS1 gene was subsequently performed on fungal isolates obtained from 38 culture-positive samples, followed by species-specific amplification to identify Microsporum canis and Trichophyton rubrum. For molecular identification, DNA was extracted from pure cultures derived from hair, skin scrapings, and nail specimens. ITS region sequencing was also performed on two of the PCR-confirmed T. rubrum isolates. Prevalence was compared across animal species, age groups and living environments. Results Dermatophytes were detected in 25.3% (38/150) of samples. In cats, only M. canis 76% (19/25) was identified. In dogs, both M. canis (5/13) and T. rubrum (2/13) were found. This represents the first report of T. rubrum in a dog in T & uuml;rkiye, with ITS sequencing confirming > 99% identity to reference strains. Infection rates were significantly higher in animals under one year of age (p = 0.0097 ), while no statistically significant difference was observed between dogs and cats (p = 0.529). PCR and sequencing provided rapid and accurate identification. Conclusions Dermatophyte infections are more prevalent among juvenile animals and pose a growing zoonotic threat. Molecular diagnostics improve early detection and control strategies. These findings highlight the need for routine surveillance and reflect the critical importance of the One Health approach, which integrates human, animal, and environmental health to prevent and manage zoonotic disease transmission.