Browsing by Author "Golen, Gokcenur Sanioglu"

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Development of a Simple and Rapid Dna Extraction Method for Aspergillus Flavus
(Sciendo, 2024) Golen, Gokcenur Sanioglu; Akar, Kadir
Aspergillus species are known to be very important in human and domestic animal health. Aspergillus species commonly cause severe systemic and skin infections, as well as allergic lung diseases. With the development of PCR techniques, these methods are used to identify and diagnose fungi. DNA extraction from Aspergillus species is difficult because the fungal cell wall structure is very durable and complex. Fungal DNA extraction methods containing proteinase K and liquid nitrogen are widely used to break down the cell wall. However, these methods cause DNA loss during the extraction in Aspergillus species. In this study, on the contrary, the commonly used DNA extraction by means of ammonium hydroxide, which is generally used to break down chitin in DNA extraction of ticks and plants, is used. The efficiency of the cell wall lysis method from A. flavus with ammonium hydroxide was compared with methods containing proteinase K and liquid nitrogen. For this purpose, DNA extraction of A. flavus was tried using three different methods. As a result, the cell wall of A. flavus was lysed using ammonium hydroxide in this study. The obtained DNA's quality, concentration, and PCR performance were sufficient. This method has been evaluated as a faster, more straightforward, and more economical alternative.
Investigation of the Correlation Between Elisa and Serum Amyloid a in the Diagnosis of Bordetella Bronchiseptica in Dogs
(Wiley, 2025) Akar, Kadir; Golen, Gokcenur Sanioglu; Ekin, Ismail Hakki
BackgroundBordetella bronchiseptica is an essential bacterial pathogen characterized by chronic respiratory disease in dogs known as Kennel cough. The presence of causative antibodies in animals can also be detected by lipopolysaccharide antigen-based enzyme linked immunosorbent assay (ELISA). In recent years, it has been determined that there is a significant relationship between acute phase proteins and diseases, and disease follow-up can be done within the framework of this relationship.MethodsIn this study, blood sera from 150 dogs in an animal shelter in Van province were evaluated for B. bronchiseptica by the homemade ELISA method, and their correlations with serum amyloid A (SAA) were investigated. Blood serum samples were analysed for antibodies against B. bronchiseptica using a homemade ELISA method. Positive animals were also molecularly confirmed using nasal swabs by PCR. A commercial ELISA kit determined SAA levels in blood sera.ResultsEighteen (12%) of the analysed blood serum samples were found positive by the homemade ELISA method. SAA concentrations in the positive blood sera were elevated from 12.7 to <= 38.98 mg/L. SAA concentrations in blood sera serologically positive for B. bronchiseptica were statistically significant.ConclusionsIn this study, in which the relationship between SAA concentration and B. bronchiseptica was investigated for the first time in Turkey, it was concluded that SAA concentration analysis may help diagnose and monitor the disease. In addition, the presence and prevalence of this critical and zoonotic agent causing chronic respiratory tract disease in dogs in Van province was revealed for the first time in this study.
Microbial Contamination of in Vitro-Derived Cattle Embryos and Resistance Genes
(Sciendo, 2025) Golen, Gokcenur Sanioglu; Akar, Kadir; Karasahin, Tahir; Senturk, Goktug; Golen, Selcuk; Aras, Zeki
The global trend of in vitro embryo systems, particularly the in vitro fertilization (IVF) culture system, is gaining momentum. Despite the strict standards followed in in-vitro embryo procedures, microbiological contamination is occasional, and the relevant literature is scarce. In this study, for the first time, IVF culture dishes with microbial contamination and resistance genes of isolates were evaluated in veterinary medicine. Samples were microscopically taken from IVF tissue cultures suspected of bacterial or fungal contamination and sent to the microbiology laboratory for further examination. The total contamination rate was 11.1% in IVF cultures where cell division did has stopped or turbidity occurred. Identification of contaminant microorganisms showed that infections were mainly caused by E. coli 9.5% and Candida spp. 1.58%. A set containing multiplex antibiotic primers was used during the IVF protocol to determine antibiotic resistance genes. All E. coli isolates were resistant to penicillin used in the Kirby-Bauer, and 16% was resistant to streptomycin. This study is the first systematic evaluation of microbial contamination of bovine IVF culture vessels in veterinary medicine. IVF culture should be evaluated in more detail to learn more about the source of the microorganism and to develop adequate measures to prevent microbial contamination.