Browsing by Author "Guler, Emrah"

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Genetic Diversity Assessment of Promising Walnut (Juglans Regia L.) Genotypes Using RAPD, ISSR, and IPBS Markers
(Sciendo, 2025) Basak, Ibrahim; Muradoglu, Ferhad; Ozer, Goksel; Guler, Emrah
This study investigates the genetic diversity and relationships among different walnut genotypes using three molecular markers: RAPD, ISSR, and iPBS. The RAPD markers produced 23 polymorphic bands, with an average of 7.77 bands per primer and a 75.53% polymorphism rate. ISSR markers generated 22 polymorphic fragments, averaging 8.50 fragments per primer with a 75.65% polymorphism rate. iPBS markers yielded 65 bands, with 72.39% being polymorphic and an average of 11.75 fragments per primer. The PIC values were highest for RAPD markers (average 0.31), followed by ISSR (0.25) and iPBS markers (0.22). RAPD markers also had the highest resolving power (RP) with an average value of 4.93, followed by iPBS (4.80) and ISSR markers (3.90). The diversity parameters, particularly the number of effective alleles, observed heterozygosity, and expected heterozygosity, indicated that the ISSR marker system exhibits the highest genetic diversity among the walnut genotypes. The Mantel test results showed significant correlations among the marker types, especially between iPBS markers and the combined marker analysis, emphasizing the importance of integrating multiple marker systems for a comprehensive assessment of genetic diversity. Clustering and Principal Coordinates Analysis (PCoA) revealed consistent genetic relationships and clustering patterns, with certain genotypes showing close genetic affiliations across all marker systems. This study emphasizes the value of using a combination of molecular markers to gain a thorough understanding of genetic variability in walnut genotypes, essential for conservation and breeding efforts. Future research should continue to use multiple marker systems to further understand the genetic structure and diversity in walnuts and other economically important species. Diese Studie untersucht die genetische Diversit & auml;t und die Beziehungen zwischen verschiedenen Walnuss-Genotypen unter Verwendung von drei molekularen Markersystemen: RAPD, ISSR und iPBS. Die RAPD-Marker erzeugten 23 polymorphe Banden mit einem Durchschnitt von 7,77 Banden pro Primer und einer Polymorphismusrate von 75,53 %. ISSR-Marker generierten 22 polymorphe Fragmente mit durchschnittlich 8,50 Fragmenten pro Primer und einer Polymorphismusrate von 75,65 %. Die iPBS-Marker lieferten 65 Banden, von denen 72,39 % polymorph waren, bei einem Durchschnitt von 11,75 Fragmenten pro Primer. Die PIC-Werte waren bei den RAPD-Markern am h & ouml;chsten (Durchschnitt 0,31), gefolgt von ISSR (0,25) und iPBS-Markern (0,22). Die RAPD-Marker wiesen ebenfalls die h & ouml;chste Aufl & ouml;sungskraft (RP) mit einem Durchschnittswert von 4,93 auf, gefolgt von iPBS (4,80) und ISSR-Markern (3,90). Die Diversit & auml;tsparameter, insbesondere die Anzahl effektiver Allele, die beobachtete Heterozygosit & auml;t und die erwartete Heterozygosit & auml;t, zeigten, dass das ISSR-Markierungssystem die gr & ouml;ss te genetische Diversit & auml;t unter den Walnuss-Genotypen aufweist. Die Ergebnisse des Mantel-Tests ergaben signifikante Korrelationen zwischen den Markertypen, insbesondere zwischen den iPBS-Markern und der kombinierten Markeranalyse, was die Bedeutung der Heranziehung mehrerer Markersysteme f & uuml;r eine umfassende Bewertung der genetischen Diversit & auml;t unterstreicht. Clusteranalysen und Hauptkoordinatenanalysen (PCoA) zeigten konsistente genetische Beziehungen und Clusterungsmuster, wobei bestimmte Genotypen & uuml;ber alle Markersysteme hinweg enge genetische Verwandtschaften aufwiesen. Diese Studie hebt den Wert der kombinierten Anwendung verschiedener molekularer Marker hervor, um ein umfassendes Verst & auml;ndnis der genetischen Variabilit & auml;t bei Walnuss-Genotypen zu erlangen, was f & uuml;r Erhaltungs- und Z & uuml;chtungsprogramme von entscheidender Bedeutung ist. Zuk & uuml;nftige Forschung sollte weiterhin mehrere Markersysteme einsetzen, um die genetische Struktur und Diversit & auml;t bei Waln & uuml;ssen und anderen wirtschaftlich wichtigen Arten besser zu verstehen.
Preserving Quality and Extending Shelf Life of Climacteric Persimmon Fruits Using Melatonin and Modified Atmosphere Packaging
(Wiley, 2025) Kucuker, Emine; Gundogdu, Muttalip; Guler, Emrah; Sumbul, Ahmet; Tekin, Onur; Hallac, Bulent
Quality and product losses during the postharvest storage of climacteric persimmon fruits pose significant challenges due to their short shelf life and limited marketing period. This study examined the effects of melatonin (1 mM) and modified atmosphere packaging (MAP) on preserving the quality, biochemical properties, and organic acid contents of persimmons, as well as extending their shelf life during storage at 0 degrees C +/- 0.5 degrees C. The MAP resulted in the lowest weight loss, recording only 6.63%. The combination of melatonin and MAP provided the highest fruit firmness at 8.69 kg/cm2, along with a total antioxidant level of 12.40 mu mol TE 100 g-1. Additionally, this treatment improved total phenolic content and most individual organic acids, except for fumaric acid, which was highest in the MAP treatment. The organic acid contents of the fruits varied during storage, depending on the specific acid. Malic acid was the predominant organic acid and was consistently better preserved by the treatments compared to the control. It is important to note that the reduction in malic acid was approximately 1.5 times less in the treated fruits than in the controls, while the decline of other organic acids was 3-4 times greater. In conclusion, both MAP and the combination of melatonin and MAP were effective methods for preserving the quality attributes and extending the shelf life of persimmon fruits.
Use of Spermidine To Preserve Organic Acids, Polyphenols, and Quality of Cold Stored Plum Fruits
(Academic Press inc Elsevier Science, 2023) Gundogdu, Muttalip; Guler, Emrah; Aglar, Erdal; Arslan, Tuncer; Kan, Tuncay; Celik, Kenan
One of the primary goals of fruit production and storage is to ensure pre-and post-harvest food quality. Polyamines have recently been used to improve food quality after harvest. We used spermidine concentrations of 0.5, 1, and 1.5 mM in plum fruits for 20 and 40 days of cold storage. Each dose provided significantly higher preservation of sensory properties, organic acids, and phenolic compounds than the control, with a gradual improvement by doses. Malic acid was the predominant organic acid and was gradually preserved by spermidine doses, including 25% more malic acid for the 1.5 mM spermidine treatment. Similarly, chlorogenic acid, the most abundant phenolic compound studied, was also 25% higher when the spermidine dose rose to 1.5 mM. The 1.5 mM spermidine notably inhibited weight loss and respiration rate. Organic acids and phenolic compounds were particularly protected by the 1 and 1.5 mM doses, and multivariate approaches suggested that spermidine has a comprehensive stability effect on secondary metabolites. This study proposes spermidine as a comprehensive metabolic preserver that protects polyphenols and organic acids, thus preserving the taste and healthiness of cold-stored plum fruits.