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Browsing by Author "Gulhan, T"

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    Article
    Brucellosis in Horses Around Van, Turkey
    (indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Aksakal, A; Gulhan, T; Boynukara, B
    A programme is being carried out in Turkey to eradicate brucellosis from cattle by compulsory slaughtering of positive cases on serological test surveillance. In spite of the very strict application of the programme, the results obtained so far are not satisfactory in respect of eliminating the disease. It seems that it will be very difficult to achieve this goal without considering the disease status in other domestic animals. Brucella abortus is associated with chronic inflammatory conditions including fistulous withers, poll-evil and also joint infection in horses. Consequently, in this study, to evaluate prevalence of brucellosis in horses, a comparative study of standard tube agglutination test (STAT) and Rose Bengal Plate Agglutination Test (RBPT) were carried out on horse sera.
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    The Characterization of Protein Profiles of the Isolated Aeromonas Sobria Strains From Animal Faeces by Sds-Page
    (indian veterinary Journal, 2004) Boynukara, B; Korkoca, H; Senler, NG; Gulhan, T; Atalan, E
    Aeromonas spp. are members of the Vibrionaceae (Janda, 1991). Although they are common in fresh surface water, their presence in faeces of several animals and humans has been proved (Boynukara et al., 2002; Jindal et al., 1993). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins have been used for typing several bacterial species, including Aeromonads (Stephenson and Tabaqchali 1986; Stephenson et al., 1987). A more sensible approach might be to identify isolates as A. hydrophila, A. sobria or A. caviae, and use an electrophoretic fingerprinting technique to further characterize them (Popoff, 1984), Maclnnes et al. (1979) did the first DNA hybridization experiments with Aeromonas, and concluded that the genus consist of two main evolutionary lines; a diverse group of motile Aeromonads and genetically more homogeneous non-motile Aeromonads. Fanning et al. (1985) confirmed that all strains of Aeromonas were more closely related to each other than to species in other families, and found that motile Aeromonas species could be divided into at least 10 different DNA hybridization. In this study, we aimed to characterize the Aeromonas sobria isolated from Turkish Van cat, chicken, gull and Rainbow trout and identified according to Popoff (1984) using SDS-PAGE method.