Browsing by Author "Gur, Tugba"

Now showing 1 - 15 of 15

Assessment of Therapeutic Potential of Silver Nanoparticles Synthesized by Ferula Pseudalliacea Rech. F. Plant
(Elsevier, 2022) Kocak, Yilmaz; Oto, Gokhan; Meydan, Ismet; Seckin, Hamdullah; Gur, Tugba; Aygun, Aysenur; Sen, Fatih
Plant-mediated synthesis of silver nanoparticles (Ag NPs) has increased its use in various biomedical applications due to its cost-effectiveness, renewable and environmentally friendly properties. Therefore, the focus of the study was to synthesize Ag NPs and to evaluate their antibacterial, and antioxidant activities by using the aqueous root extract of Ferula pseudodalliacea plant as a reducing agent. In addition, the synthesis of nanoparticles was confirmed by performing Ag NPs/Fp characterization processes. According to the findings of our research, color change due to surface plasmon resonance was confirmed by UV-vis spectrometry. The crystal properties of Ag NPs/Fp were determined according to the XRD model. Phytochemicals responsible for coating and reduction of Ag NPs/Fp were observed by FT-IR analysis. It has been shown that the therapeutic effect of Ag NPs/Fp exhibits better antimicrobial and antioxidant activity than aqueous extract. In addition, it was determined that Ag NPs/Fp structures showed the best antifungal effect against Candida albicans ATTC 90028 pathogen and gave a better zone than Rifampicin antibiotic. Therefore, studies at the molecular level and more comprehensively are required to determine the bioactive components and pharmacological effects of Ferula pseudodalliacea plant to confirm our antibacterial, antioxidant, and antifungal activity results.
Characterization of Rheum Ribes With Zno Nanoparticle and Its Antidiabetic, Antibacterial, Dna Damage Prevention and Lipid Peroxidation Prevention Activity of in Vitro
(Academic Press inc Elsevier Science, 2022) Meydan, Ismet; Burhan, Hakan; Gur, Tugba; Seckin, Hamdullah; Tanhaei, Bahareh; Sen, Fatih
This study aims to investigate the antidiabetic, antimicrobial, DNA damage, and lipid peroxidation prevention activity of ZnO NPs/Rr formed as a result of the interaction of Rheum ribes (R.ribes) plant with ZnO. The ZnO NPs/Rr obtained as a result of the reaction were confirmed using high-reliability characterization methods. According to the data obtained as a result of the study, it is seen that the activity of ZnO NPs/Rr to prevent lipid peroxidation is quite strong. Lipid peroxidation inhibition activity of ZnO NPs/Rr at the highest concentration of 250 mu g/ml was calculated as % 89.1028. It was observed that ZnO NPs/Rr prevented DNA damage by % 92.1240 at the highest concentration of 100 mu g/ml. It was determined that the antidiabetic effect of ZnO NPs/Rr formed by ZnO of R. ribes plant, which is used as a medicinal plant as an antidiabetic, was significant. It appears to have a strong antidiabetic property compared to the positive control acarbose. In our current study, it was observed that ZnO NPs/Rr formed zones ranging from 8 +/- 3.0 to 21 +/- 4.5 against Gram-positive and Gram-negative microorganisms. It has been determined that ZnO nanoparticles have an antibacterial effect.
Chitosan/Pva-supported Silver Nanoparticles for Azo Dyes Removal: Fabrication, Characterization, and Assessment of Antioxidant Activity
(Royal Soc Chemistry, 2024) Meydan, Ismet; Aygun, Aysenur; Tiri, Rima Nour Elhouda; Gur, Tugba; Kocak, Yilmaz; Seckin, Hamdullah; Sen, Fatih
With the advancement of technology, studies in the field of nanotechnology have attracted great interest in recent years. The fact that nanomaterials have superior advantages over micromaterials provides a wide range of uses. Green synthesis is an effective way to prepare nanomaterials with an easy, fast, and environmentally friendly method. Within the scope of the study, AgNPs were synthesized using basil extract and combined with chitosan/PVA as a support material. By using chitosan/PVA support materials, the surface area of AgNPs was increased and it was aimed to improve their properties. The synthesized AgNPs@chitosan/PVA nanocomposite was characterized using various methods. In the UV-Vis spectrum, an absorbance peak was observed at 430 nm for the AgNPs@chitosan/PVA nanocomposite, and the particle size was determined as 25.10 nm according to TEM results. In addition, the photocatalytic and antioxidant activities of AgNPs@chitosan/PVA nanocomposite were investigated. The antioxidant activity of the AgNPs@chitosan/PVA (100 mu g mL-1) nanocomposite against DPPH and H2O2 was determined as 89.18% and 71.87%, respectively. The photocatalytic activity of the AgNPs@chitosan/PVA nanocomposite against methylene blue (MB), methylene red (MR), methylene orange (MO), safranin, and crystal violet (CV) dyes was 77%, 85%, 79%, 54%, and 9%, respectively. While the highest photocatalytic activity was observed against MR dye, very low photocatalytic activity was observed for CV. In light of the results obtained, it can be said that the AgNPs@chitosan/PVA nanocomposite has the potential to be used as an antioxidant agent and photocatalyst. The photocatalytic activity of AgNPs@chitosan/PVA nanocomposite was tested against different dyes such as methylene blue, methylene orange, and methylene red as an effective photocatalyst.
Exploring Thermodynamics and Kinetic Parameters of Immobilized Catalase Enzyme Via Adsorption on Krill Clay
(desalination Publ, 2017) Nas, Mehmet Salih; Gur, Aycan; Gur, Tugba; Yonten, Vahap
The thermodynamic parameters and immobilization kinetics of catalase enzyme via adsorption onto krill clay were performed in aqueous solution in batch system. The adsorbent was characterized by X-ray fluorescence, scanning electron microscope and Brunauer-Emmett-Teller N-2 adsorption measurements. The effect of some parameters to the adsorption process such as contact time, stirring speed, initial enzyme concentration, initial ionic strength, temperature and pH were analyzed. Maximum adsorption capacity (q(m)) showed a great dependence on pH and initial enzyme concentration. It was found at pH 5.5, and initial enzyme concentration, 0.0075 g L-1 values. Pseudo-first-order, pseudo-second-order and intraparticle diffusion were used to fit the kinetics data on kinetic models. Furthermore, thermodynamic activation parameters such as enthalpy, entropy, Gibbs free energy and activation energy (Delta H degrees, Delta S degrees, Delta G degrees and E-a) which reveal that catalase enzyme adsorption occur spontaneously and in endothermic nature were determined. The obtained results confirmed that the applicability of krill clay is an efficient immobilizing supporter for catalase enzyme and krill clay material can be used as a very effective adsorbent in immobilization of this enzyme from aqueous solutions.
Facile Bio-Fabrication of Pd-Ag Bimetallic Nanoparticles and Its Performance in Catalytic and Pharmaceutical Applications: Hydrogen Production and In-Vitro Antibacterial, Anticancer Activities, and Model Development
(Elsevier, 2022) Gulbagca, Fulya; Aygun, Aysenur; Altuner, Elif Esra; Bekmezci, Muhammed; Gur, Tugba; Sen, Fatih; Vasseghian, Yasser
The production of nanoparticles by the biosynthesis method attracts great attention due to their environmentally friendly structure and biocompatibility. In this study, a green method for the synthesis of Palladium-Silver nanoparticles (Pd-Ag NPs) using the extract of Nigella satioa seeds is reported. Pd-Ag NPs obtained by the green synthesis method were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), UV-vis spectrometry, and X-Ray diffraction patterns (XRD). Pd-Ag NPs were seen to have a spherical structure in the TEM analysis image and the average particle size was found to be 6.80 nm. In addition, the anticancer and antibacterial activities of Pd-Ag NPs synthesized by the green synthesis method were investigated. Pd-Ag NPs had lethality of 69.26%, 52.28%, 76.90%, and 57.49% respectively, against Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Bacillus subtilis, and Escherichia coli bacteria at 200 mu g/mL. Besides, the antibacterial activity of Pd-Ag NPs against B. subtilis, S. aureus, and MRSA bacteria was developed by the Neuro-fuzzy (ANFIS) model. The minimum inhibitory concentration (IC50) values of Pd-Ag NPs against human breast cancer cells, human endometrial carcinoma cells, and human cervical cancer cell lines were determined as 12.4384 +/- 0.39 mu g/mL, 13.5043 +/- 0.539 mu g/mL, 17.7172 +/- 0.782 mu g/mL, respectively. The catalytic activity of Pd-Ag NPs was investigated by sodium borohydride (NaBH4) hydrolysis. Enthalpy, entropy, turner of frequency (TOF), and activation energy values were calculated as 24.51 kJ/mol, -183.15 J/mol.K, 1387.29 h(-1), 27.01 kJ/mol, respectively. In the light of the obtained results, it promises that Pd-Ag NPs may play a therapeutic role in complications related to cancer and bacterial infections. The use of Pd-Ag NPs as catalysts will contribute to the development and application of new nano-catalysts to reduce environmental pollution. (C) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.
Facile Synthesis of Biogenic Palladium Nanoparticles Using Biomass Strategy and Application as Photocatalyst Degradation for Textile Dye Pollutants and Their In-Vitro Antimicrobial Activity
(Pergamon-elsevier Science Ltd, 2022) Liang, Yunyi; Demir, Halit; Wu, Yingji; Aygun, Aysenur; Tiri, Rima Nour Elhouda; Gur, Tugba; Vasseghian, Yasser
Among biological applications, plant-mediated Pd NPs for multi-drug resistance (MDR) developed in pathogenic bacteria were synthesized with the help of biomass of lemon peel, a biological material, with a non-toxic, environmentally friendly, human-nature green synthesis method. Characterization of synthesized Pd NPs was carried out by UV-Vis spectrometry, Transmissive Electron Microscopy (TEM), X-ray diffraction (XRD), and
Green Synthesis and Characterization of Camellia Sinensis Mediated Silver Nanoparticles for Antibacterial Ceramic Applications
(Elsevier Science Sa, 2020) Gol, Fatma; Aygun, Aysenur; Seyrankaya, Abdullah; Gur, Tugba; Yenikaya, Cengiz; Sen, Fatih
This study focuses on the use of green synthesized silver nanoparticles (Ag-NPs) with the aid of Camellia sinensis (black tea) extract to provide antibacterial activity on ceramic structure. The synthesized Ag nanoparticles were added to the glaze used in the ceramic structures and mixed homogeneously. The homogeneous mixture was characterized by transmission electron microscopy (TEM), X-Ray Diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and UV-Vis spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR) techniques. The SPR band of the synthesized biogenic Ag NPs was observed as 422 nm during the reaction at mom temperature. TEM analysis revealed that Ag NPs were spherical and a particle size between 10 and 20 nm. Furthermore, the antibacterial properties of the homogeneous mixture (Ag NPs and glaze) were tested against Escherichia coli (E. coli, Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Biogenic Ag NPs at a concentration of 100 mu g/ml were observed to have 90%, 75%, 75%, 80% lethal effects against S. aureus, MRSA, B. Subtilis, and E. Coli bacteria, respectively. The antibacterial results of Ag NPs obtained with the help of Camellia sinensis show that they may have potential application and development in the field of ceramics. In addition, the antibacterial activity of commercially available antibiotics and the prepared Ag NPs were analyzed in ceramic glazes.
Green Synthesis and Characterization of Camellia Sinensis Mediated Silver Nanoparticles for Antibacterial Ceramic Applications (Vol 250, 123037, 2020)
(Elsevier Science Sa, 2021) Gol, Fatma; Aygun, Aysenur; Seyrankaya, Abdullah; Gur, Tugba; Yenikaya, Cengiz; Sen, Fatih
Green Synthesis, Characterization and Bioactivity of Biogenic Zinc Oxide Nanoparticles
(Academic Press inc Elsevier Science, 2022) Gur, Tugba; Meydan, Ismet; Seckin, Hamdullah; Bekmezci, Muhammed; Sen, Fatih
In this study, we tried to enlighten the structure of zinc oxide nanoparticles (ZnO NPs) obtained from Thymbra Spicata L. plant by using green synthesis method in various ways. Some properties of zinc oxide nanoparticles were determined by using the characterization methods that scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDX), fouirer transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet visible spectroscopy (UV-Vis) spectroscopy methods. The detected Zn nanoparticle sizes were determined to be between 6.5 nm and 7.5 nm. In addition to these studies, we investigated the antimicrobial effects of zinc oxide nanoparticles obtained by green synthesis against some pathogens. According to the results, it was seen that zinc oxide nanoparticles formed zones with a diameter of 16.3 mm, 10.25 mm, 13 mm and 10.2 mm, respectively, against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 bacteria and Candida albicans ATTC 90028 fungus, respectively. However, the radical quenching activity (DPPH) of the nanoparticles (Ts-ZnONP (79.67%)) was determined to be quite good compared to the positive control BHA. In addition, it is seen that the protective effect of ZnO NPs against DNA damage increases depending on the concentration. At a concentration of 100 mg/L, the DNA damage inhibitory effect was found to be maximum. In line with the comprehensive results, it was determined that the zinc oxide nanoparticles obtained with the green synthesis method have the potential of use in a wide variety of fields.
Green Synthesis, Characterizations of Silver Nanoparticles Using Sumac (Rhus Coriaria L.) Plant Extract and Their Antimicrobial and Dna Damage Protective Effects
(Frontiers Media Sa, 2022) Gur, Tugba
Today, antimicrobial resistance against bacteria has become an important global public health problem. In this sense, the development of new biomedical solutions is becoming increasingly important. Especially plant-based nanoparticles produced by green synthesis are used in many fields. AgNPs have an important place in nanoscience and nanotechnology, especially in nanomedicine. Therefore, the present study was conducted to synthesize AgNPs using the medicinal plant extract sumac and to characterize them using advanced techniques and to determine the antibacterial activity of some bacteria that cause disease. Nanoparticles produced by green synthesis are used in a wide area around the world due to their many advantages such as environmentally friendly, economically and non-toxically. In this study, AgNPs were biosynthesized using sumac extract and evaluated for their antibacterial potency against Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Pseudomonas aeruginos, and Candida albicans. UV-Vis spectroscopy of the prepared sumac-mediated silver solution showed the absorption maximum at about 400 nm. According to the TEM results obtained, it was observed that the particles were spherical, approximately 4 nm in size, and showed a homogeneous distribution. The sizes of nanoparticles formed by XRD pattern were supported and silver nanoparticles were obtained. According to the obtained XRD results, the crystal nature of nanoparticles in face-centered cubic structure was confirmed by the peaks in the XRD model corresponding to the planes (111) (200) (220) and (311). It was observed that the synthesized AgNPs provided a strong protection against plasmid DNA damage. It was determined that the inhibition zone diameters of biosynthesized nanoparticles measured in terms of antibacterial activity were between 10 and 14 mm. As a result, the study revealed significant antibacterial activity of the synthesized AgNPs due to extensive membrane damage.
Highly Active Pdpt Bimetallic Nanoparticles Synthesized by One-Step Bioreduction Method: Characterizations, Anticancer, Antibacterial Activities and Evaluation of Their Catalytic Effect for Hydrogen Generation
(Pergamon-elsevier Science Ltd, 2023) Aygun, Aysenur; Gulbagca, Fulya; Altuner, Elif Esra; Bekmezci, Muhammed; Gur, Tugba; Karimi-Maleh, Hassan; Sen, Fatih
Metallic nanoparticles (MNPs) have important applications in medicine and technology. Bimetallic NPs, which are among the metallic nanoparticles, are of great interest due to their properties. An innovative method by green synthesis has been developed to obtain bimetallic NPs. Aromatic plants are used in this synthesis method. One of the plants used for green synthesis is Nigella sativa and it has a unique place among plants for use as medicine. In this study, the synthesis of Palladium-Platinum bimetallic nanoparticles (PdPt NPs) and the catalytic, antibacterial, and anticancer activity of synthesized PdPt NPs by green synthesis method using Nigella sativa seed extract are reported. The synthesized PdPt NPs were characterized by Fourier Transform Infrared Spectrophotometer (FTIR), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), and UV-Vis spectrom-etry techniques. The catalytic activity of PdPt NPs was determined by performing sodium borohydride (NaBH4) hydrolysis experiments. According to the results obtained, Turnover Frequency (TOF), activation energy, entropy, and enthalpy values were found to be 1664.76 h-1, 13.93 kJ/mol,-119.02 J/mol.K, and 11.43 kJ/mol, respectively. It was determined that PdPt NPs are highly effective catalysts for hydrogen production. PdPt NPs (200 mu g/mL) were determined to have antibacterial activity of 57.58%, 64.42%, 48.68%, and 58.77% against Escherichia coli, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, and Bacillus subtilis bacteria, respectively. In addition, the cytotoxic effects of PdPt NPs, MTT against human breast cancer cell line (MDA-MB-231), human endometrial carcinoma cell line (Ishikawa, ISH), human cervical cancer cell line (HeLa), L929-Murine fibroblast cell line test, and IC50 values were calculated. The IC50 values of PdPt NPs applied against MDA-MB-231, ISH, and HeLa cancer cell lines were calculated as 9.1744 +/- 1.566 mu g/mL, 12.2431 +/- 1.132 mu g/ mL, 18.1963 +/- 1.730 mu g/mL, respectively. No significant cytotoxic effect was observed in healthy L929-murine fibroblasts. Green synthesis of PdPt NPs was determined to have significant advantages over chemical approaches. The biogenic PdPt NPs synthesized in this study suggest the design of bio-based bimetallic catalysts with high catalytic perfor-mance to prevent environmental pollution. (c) 2021 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
Homocysteine Levels and Lipid Profile in Hemodialysis Patients
(Ortadogu Ad Pres & Publ Co, 2007) Dulger, Haluk; Gur, Tugba; Sayarlioglu, Hayriye; Sekeroglu, Ramazan; Erkoc, Reha; Begenik, Hueseyin
Objective: The aim of this study was to investigate the levels of homocysteine and lipid profiles in patients included in hemodialysis programs for chronic renal failure (CRF). Material and Methods: The patients were divided into 2 groups and each group consisted of 30 patients, leading to a total of 60 patients. On the other hand, 20 healthy people comprised the control group. The first group (nontreated CRF) included new patients without a previous history of dialysis or any medical treatment; the second group (treated CRF) included patients who have received medical treatment [vitamin 13,2 (1 mg/month) and folic acid (15 mg/week)] and dialysis for at least the last 5 years. Serum total homocysteine, vitamin B-12, folic acid, creatinine, triglyceride, total cholesterol, HDL, LDL, VLDL, cholesterol levels were measured in all patients and the control group. Results: Levels of homocysteine were high in group I and group 2 patients (respectively p < 0.01, p < 0.05), whereas serum HDL cholesterol levels in group 2 were low compared to those in the control group (p < 0.01). In addition, a negative correlation was observed between homocysteine and folic acid levels in group 2 patients (r= -0.48, p < 0.01). Conclusion: The results of this study showed that homocysteine levels in CRF increased and this increase was lower in group 2 patients. Administration of folic acid reduced the levels of homocycstein. Thus, we Suggest that folic acid may be a significant factor to prevent the progression of chronic renal failure.
Investigation of Adsorption Kinetics and Removal of Copper (Ii) and Zinc From Aqueous Solution by Bardakci Clay
(Amer Chemical Soc, 2009) Gur, Aycan; Y'ld'z, Ardnan; Alkan, Erkan; Gur, Tugba
Tumor Markers and Biochemical Parameters in Colon Cancer Patients Before and After Chemotherapy
(Asian Pacific Organization Cancer Prevention, 2011) Gur, Tugba; Demir, Halit; Kotan, M. Cetin
Objectives: Tumor markers and biochemical parameters were investigated in patients suffering from colon cancer and in volunteer healthy persons. Design and Methods: Biochemical analyses of erythrocyte enzymes and serum values for series of established tumor markers were conducted for 40 patients diagnosed with colon cancer, before and after chemotherapy, and 29 healthy volunteers between 2008 and 2009. Results: Activities of catalase and carbonic anhydrase were lower in sick than healthy groups (p<0.05) before and after chemotherapy, while alkaline phosphatase and creatinine were increased. The patients with colon cancer showed significant (p<0.05) elevation of CRP, fibrinogen, CA125, AFP, and decrease in ferritinin, before but not after therapy. Conclusion: The results obtaining from this investigation can be useful for colon cancer risk assessment.
Vitamin B12, Folate Levels and Somatoform Dissociation in Conversion Disorder
(Pakistan Medical Assoc, 2020) Ozdemir, Pinar Guzel; Gur, Tugba; Cokluk, Erdem; Isik, Mesut; Tapan, Suheda
Objective: To evaluate the association of folate and vitamin B12 levels in patients with conversion disorder, and to illuminate the aetiology of conversion disorder by examining depression and somatoform dissociation. Method: The case-control study was conducted from March 2014 to May 2015 at the Medical Centre of Yuzuncu Yil University, Van, Turkey, and comprised patients diagnosed with conversion disorder and healthy controls. Blood samples were taken from both groups for vitamin B12 and folate levels. Data was collected using the Beck Depression Inventory and Somatoform Dissociation Scale. Data was analysed using SPSS 18. Results: Of the 100 subjects, 55(55%) were cases with a mean age of 27.05 +/- 9.04 years and 45(45%) were controls with a mean age of 26.56 +/- 5.96 years. The mean level of B12 was 283.93 +/- 122.96 in cases and 324.62 +/- 128.82 in controls (p=0.05). The mean level of folic acid was 5.47 +/- 1.84 in cases and 6.07 +/- 2.26 in controls (p>0.05). Conclusions: Physicians need to be vigilant about vitamin B12 levels in patients with conversion symptoms.