Browsing by Author "Gurler, Sukru"

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Genetic Variability of Csn1s1 Gene in Goat Populations Raised in Southeastern Region of Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2013) Bozkaya, Faruk; Gurler, Sukru; Yerturk, Mugdat
The objective of this study was to investigate genetic variability of CSN1S1 gene coding for alpha-s1-casein in goat populations raised in Southeastern Region of Turkey. Blood samples were collected from goats raised in Kilis(n=60), Sanliurfa (n=56), and Siirt (n=55) provinces of Turkey. From the blood samples DNA was isolated by using phenol-chloroform extraction. Genotypes of animals were determined by using polymerase chain reaction (PCR), allele specific PCR or PCR and restriction fragment length polymorphism methods. In Kilis and Sanliurfa populations CSN1S1 A*, B*, F and N alleles were observed, while in Siirt population only A* and B* alleles were found. Frequencies of A*, B*, F and N alleles were 0.375, 0.367, 0.017 and 0.242 in Kills, 0.632, 0.208, 0.009 and 0.151 in Sanliurfa and 0.782, 0.218, 0.000 and 0.000 in Siirt populations, respectively. CSN1S1 E and 01 alleles were not observed among the populations studied. Observed and expected genotype frequencies did not differ significantly (P>0.05). The results of this study suggested that there were sufficient genetic variability of CSN1S1 gene especially in Sanliurfa and Kilis populations in order to select individuals for different breeding purposes.
Investigation on the Polymorphism of Some Loci by Using Pcr-Rflp in Japanese Quails (Coturnix Coturnix Japonica) Raised in Different Locations of Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2013) Bozkaya, Faruk; Gurler, Sukru; Yerturk, Mugdat
The aim of this study was to investigate the restriction fragment length polymorphism (RFLP) of some loci on chromosomes 1 (CJA1) and 3 (CJA3) in Japanese quails (Coturnix coturnix japonica) raised in Turkey. With this study population genetics parameters were estimated in order to select individuals for establishing a reference population, which would be used for studies on recombination frequency. Fertilized eggs obtained from flocks raised in six different provinces of Turkey were incubated for 120 h and 191 embryos were collected. From the tissue samples of the embryos, DNA was isolated by using DNA isolation kits. Particular regions of SEMA3E, IFR1, HAL, LOC396025, UGP2, LOC396192, TLX and BMP5 loci were amplified with specifically designed primers for each locus by using PCR technique. The PCR products were cut with an appropriate restriction enzyme for each locus and analysed by using agarose gel electrophoresis. Presence of different alleles, allele and genotype frequencies, heterozygosities and genetic distances were estimated. Out of the eight loci studied, polymorphism was found for the SEMA3E and TLX loci on 1. and 3. chromosomes, respectively, while five loci were found to be monomorphic and one locus (HAL) could not be amplified by PCR. The populations studied were found to be mostly in Hardy-Weinberg equilibrium. The results indicated that the SEMA3E and TLX loci can be used for studying recombination frequencies in the populations included into the study.