Browsing by Author "Inal, Behcet"

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Antioxidant Capacity and Phylogenetic Analysis of Twenty Native Grape Cultivars in Siirt Province, Turkey
(C M B Assoc, 2018) Fidan, Mehmet; Erez, Mehmet Emre; Inal, Behcet; Pinar, Suleyman Mesut; Altintas, Serdar
The quality of grape cultivars not only depends on the grape cultrvar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultivars were imestigated to construct their phylogenetic tree. According to reported data on antioxidant activity and content of phytochemicals, all cultivars exhibited different values from each other, but Rutik and Gad?v cultivars were found as significantly higher in comparison to others According to bioinformatics analysis, twenty grape cultivars were distributed into six different major groups. Rutik and Sevkeye cultivars exhibit significant distinction from other grape cultivars. The phylogenetic analysis was also associated and supported with the results of obtained data from bioactivity. The bioactivity and phylogenetic analysis were firstly identified and quantified in these grape cultivars, however, with regard to obtained data from the current study, the grape cultivars grown in Siirt province were indicated significant and valuable results and as a result, these cultivars have to be evaluated before extinction.
Bryonia Multiflora Extract Induces Autophagy Via Regulating Long Non-Coding Rnas in Breast Cancer Cells
(Routledge Journals, Taylor & Francis Ltd, 2021) Tokgun, Onur; Tokgun, Pervin Elvan; Turel, Samet; Inal, Behcet; Inci, Kubilay; Tan, Secil; Can Alvur, Ozge
Bryonia multiflora, one of the species of Bryonia L. (Cucurbitaceae) genus, is a perennial, dioecious, herbaceous plant with rhizome-shaped roots. Bryonia species have anti-inflammatory, antimicrobial, cytotoxic, antioxidant, etc., activities and their components consume antitumoral effects. Purpose of the study to investigate the effect of Bryonia Multiflora extract (BMST) on breast cancer cells. Our results revealed that MCF-7 and MDA-MB-231 cells underwent significant morphological changes leading to cell rounding. No significant changes were observed in the cell viability by MTT. Acridine orange staining of our cells gave rise to think that BMST might lead our cells to autophagy. Therefore, possible molecular mechanisms underlying morphological changes such as autophagy (LC-3B, Beclin, AMBRA1) and apoptosis (Bcl-2) were evaluated on mRNA and protein levels. BMST treated MCF-7 and MDA-MB-231 cells had increased levels of autophagy markers whereas decreased levels of Bcl-2. p21 levels were also found to be increased in both cells. Analysis of lncRNA expressions has shown that BMST treatment led to changes in the expression levels of several lncRNAs playing roles in autophagy. The current study has shown that BMST induces autophagy in MCF-7 and MDA-MB-231 cells via regulating the lncRNAs revealing that BMST could be a promising therapeutic agent.
Evaluation of Mterf Gene Expressions in Response To Phytophthora Capsici Infection in Pepper (Capsicum Annuum L.) Cultivars
(Academic Press Ltd- Elsevier Science Ltd, 2024) Altintas, Serdar; Kuru, Ibrahim Selcuk; Erez, Mehmet Emre; Catkin, Sedriye; Inal, Behcet; Bingol, Omer
Despite resistance to abiotic stresses, some plant species may still show low resistance to pathogens. This biotic stress resistance can provide advantage and increase productivity in agricultural systems. However, while there is extensive research on the pathways involved in abiotic stress tolerance, the impact on the molecular mechanisms underlying pathogen resistance remains poorly understood. Among the pathogens , Phytophthora capsici , an oomycete, causes severe damage as plant pathogen, leading to blight and fruit rot in pepper and other commercially valuable crops. This study investigates the effects of Phytophthora capsici infection on highly resistant (CM334) and susceptible (Sera Demre) pepper varieties. We examined biochemical changes and gene expression across different vegetative periods (seedling, flowering, and fruiting stages). Specifically, we analyzed biochemical pathways and the mitochondrial transcription termination factor (mTERF) gene regions associated with pathogenicity. In pepper seedlings infected with P. capsici , peroxide levels increased at all developmental stages compared to control groups. Additionally, we observed a rise in H 2 O 2 content, a molecule involved in signal transduction, likely due to the oxidative stress caused by the pathogen. The expression patterns of mTERF genes varied significantly depending on both pathogen application and the developmental stage of the plants. In the seedling stage, inoculation with P. capcici resulted in decreased expression of TERF2, mTERF6, and mTERF8 genes in leaf parts of both resistant and susceptible varieties. However, mTERF28 expression showed a significant increase. Interestingly, mTERF14 and mTERF27 gene expressions increased in the resistant variety but decreased in the susceptible one. These findings, although not providing a complete picture of host resistance mechanisms, highlight the crucial role of specific plant genes in the defense pathways against Phytophthora capsici . This knowledge can contribute to selecting pepper varieties with sustainable resistance to this devastating pathogen.
Genetic Diversity Among Some Asparagus Species Using Rdna Its, Cpdna Trnl Intron Sequence and Screening for Antioxidant Activity
(Hard, 2019) Altintas, Serdar; Pakyurek, Mine; Sensoy, Suat; Erez, M. Emre; Inal, Behcet
Three species of genus Asparagus native to Lake Van Basin of Turkey were analyzed using internal transcribed spacer (ITS) and cpDNA trnL intron sequence. Furthermore, these species were screened for their antioxidant activity and total phenolic and flavonoid contents. Phylogenetic analysis based on ITS data revealed two clades: the first clade consisted of Asparagus persicus and Asparagus officinalis, and clade II consisted of Asparagus palaestinus. Similarly, phylogenetic analysis using trnL intron sequence revealed two clades. In term of total phenolic content and total flavonoid content, the highest value were measured for acetone extract of Asparagus persicus sample No. 2 and Asparagus persicus sample No. 6, respectively. The highest antioxidant activity using FRAP assay was found for Asparagus persicus sample No. 3. We concluded that these species could be useful for increasing genetic diversity among cultivated species and for breeding.
Molecular Characterization of Agonoscena Pistaciae Burckhardt and Lauterer (Hemiptera Psyllidae) Populations Spreading in Southeast Anatolia
(Springer, 2022) Dilmen, Halil; Ozgokce, Mehmet Salih; Inal, Behcet
This study aims to explain the genetic kinship relationships among populations of the psyllid species collected from several pistachio orchards in Turkey. Agonoscena pistaciae (Burckhardt and Lauterer) (Hemiptera, Psyllidae) is one of the most important pests of pistachio trees (Pistacia vera L.). This species, which continues its biological periods on pistachio trees, has been rapidly spreading in some parts of the world. Although pistachio psyllid leads to significant yield losses in pistachio trees in Gaziantep and Adiyaman in Turkey, in Siirt province such infestation has historically been very rare. However, the population density has significantly increased in Siirt province and districts in recent years. In this study, an internal transcribed spacer (ITS) DNA barcode identifier was used to determine whether these new outbreaks are a simple spread or the evolution of a new A. pistaciae biotype. In this context, by obtaining a 716-bp long ITS gene region by the polymerase chain reaction process, the genetic kinship relationships among populations of A. pistaciae in Gaziantep, Adiyaman, and Siirt provinces, which have significant potential in pistachio production, were revealed. When the data obtained were examined, no genetic differences could be detected among the population members of A. pistaciae spreading in Siirt, Gaziantep, and Adiyaman provinces. It was determined that A. pistaciae living in these regions are the same species. In addition, in the study the Wolbachia sp. type using the 16S ribosomal RNA gene region was determined.