Browsing by Author "Irmak, Mehmet"

Now showing 1 - 3 of 3

Comparison of Serum Biochemical Parameters in Ketotic and Healthy Siirt Colored Mohair Goats During Early Lactation to Identify Potential Biomarkers of Lactation Ketosis
(Univ Zulia, Facultad Ciencias Veterinarias, 2025) Irmak, Mehmet; Turgut, Ali Osman; Eroglu, Mehmet; Irak, Kivanc; Tufan, Tuncay; Koca, Davut; Unver, Ali
This study aimed to compare serum biochemical parameters between ketotic and healthy Siirt colored mohair goats during early lactation, to diagnose subclinical lactation ketosis and identify potential biomarkers. A total of 77 female goats, aged 2 to 5 years and within 30 days postpartum, were evaluated under similar management conditions. The animals were classified into two groups based on serum beta-hydroxybutyric acid levels: subclinical lactational ketosis (n = 37) and health controls (n = 40). beta-hydroxybutyric acid levels were significantly elevated in the ketosis group (0.891 +/- 0.0141 mmolL-1) compared to the control group (0.595 +/- 0.0159 mmolL-1, P<0.001), confirming its diagnostic value. Other serum parameters did not show significant differences between the groups (P>0.05). On the other hand, effect size analysis revealed that glucose level decreased in does with subclinical lactational ketosis while cholesterol increased (large effect size). The study concluded that most liver and metabolic indicators remained within normal ranges, while glucose and cholesterol levels changed due to subclinical lactational ketosis. These data indicate that feeding based on pasture under extensive conditions may be insufficient to meet the energy requirements of does in lactation and emphasize the importance of early biochemical screening during lactation for effective management of metabolic disorders during early lactation stage in goats. Therefore, it is recommended the further studies to expand the knowledge of the effect of lactational ketosis on metabolic changes in goats.
Prevalence of Subclinical Pregnancy Toxemia and Its Effect on Metabolic Profile of Ewes: A Field Study
(2025) İrak, Kıvanç; Eroglu, Mehmet; Irmak, Mehmet; Koca, Davut; Turgut, Ali Osman; Keskin, İbrahim Halil; Ünver, Ali
This study investigated the prevalence of subclinical pregnancy toxemia (SPT) in pregnant ewes and its effects on biochemical parameters. A total of 114 pregnant ewes were included in the study, and the animals were classified into two groups: healthy and SPT, based on their serum β-hydroxybutyrate (β-HBA) levels. The findings demonstrated a significant increase in β-HBA levels as pregnancy progressed (first trimester: 0.45 mmol/L; second trimester 0.58 mmol/L; third trimester: 0.74 mmol/L), indicating an elevated risk of pregnancy toxemia in later gestational stages. The most notable difference was observed in triglyceride (TRIG) levels, which significantly increased in the SPT group, reaching 51.1 mg/dL (p < 0.05). Although the aspartate aminotransferase (AST) level was not changed statistically, large effect size (0.91) indicates an increase tendency of AST in SPT group. Similarly, total bilirubin (TBILC) levels were elevated in the SPT group with moderate effect size (0.61). No significant differences were found between groups in terms of glucose (GLUC), cholesterol (CHOL), total protein (TP), albumin (ALB), gamma-glutamyl transferase (GGT), or alanine aminotransferase (ALT) levels. These results suggest that SPT exhibits a subclinical nature and causes limited hepatic involvement during the early stages. In conclusion, TRIG levels emerge as a potential supportive biomarker in addition to serum β-HBA for the early diagnosis of subclinical pregnancy toxemia. Therefore, routine monitoring of these parameters may be beneficial for the early detection of SPT and for taking preventive measures before the development of clinical symptoms.
Subclinical Pregnancy Toxemia Affects Blood Parameters of Ewes and Impairs Postnatal Growth and Development of Lambs
(Wiley, 2025) Turgut, Ali Osman; Kucuk, Mursel; Irmak, Mehmet; Ozcan, Cahit; Koca, Davut; Gulendag, Erman; Keskin, Ibrahim Halil
Pregnancy toxemia (PT) is a common metabolic disorder that occurs in the last trimester of pregnancy. This study aimed to detect the effects of subclinical PT (SPT) on serum biochemical parameters of ewes and postnatal growth and development of lambs in Romanov sheep. A total of 40 ewes and 69 lambs were included in the study. Blood samples of ewes were collected at the time of parturition to evaluate serum beta-hydroxybutyric acid (beta HBA), serum nonesterified fatty acid (NEFA), cholesterol, and triglyceride. Live weight and body measurements of lambs were recorded at birth (0th day), 30th day, and 60th (weaning) day. In the diagnosis of SPT serum beta HBA concentrations were taken into account. A cut-off value of 0.8 mmol/L for beta HBA was a critical concentration and ewes with 0.8-1.6 mmol/L beta HBA were evaluated as SPT. There were no ewes with Beta HBA value higher than 1.6 mmol/L (clinical PT) in the study. There was no significant difference in serum beta HBA, NEFA and cholesterol concentrations between ewes regarding birth type (single, twin, and triplet). However, triglyceride concentrations were higher (p < 0.001) in ewes giving birth to triplets compared to ewes giving birth to single lamb. Serum beta HBA and triglyceride concentrations were higher (p < 0.01) in ewes with SPT (n = 14) compared to healthy ewes (n = 26). There was no significant difference between ewes with SPT and healthy ewes in serum NEFA and cholesterol. Repeated measures ANOVA results indicated that SPT negatively affected 0th, 30th, and 60th body weights of twin (p < 0.01) and triplet born lambs (p < 0.05). The effect of time on body weights was also found to be significant (p < 0.001). Similarly, most of the body measurements of twin and triplet born lambs were lower (p < 0.05) in the SPT group compared to healthy group. Overall results indicate that SPT may negatively affect postnatal growth and development in Romanov lambs. Because a decline in growth performance has many negative outcomes affecting production traits in sheep, these findings may be valuable for the veterinary field.