Browsing by Author "Isler, Cafer Tayer"

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Molecular Diagnosis of Toxoplasma Gondii and Neospora Caninum in Brain Tissues of Some Wild Birds
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Muz, Mustafa Necati; Orunc Kilinc, Ozlem; Isler, Cafer Tayer; Altug, Enes; Karakavuk, Muhammet
There are limited molecular studies about Toxoplasma gondii and Neospora caninum which are economically important livestock protozoons in wild birds investigated by polymerase chain reaction (PCR) method. Molecular prevalance of both parasites in brain tissues of wild birds in Turkey is unknown. Prevalance of T. gondii was 7%, N. caninum was 14% and mix infection was found 4% in brain tissues of 101 wild birds under 20 species from two different regions of Turkey. The chi-square test has been applied to the acquired data. This is the first molecular biologic investigation for the aim of PCR diagnosis of T. gondii in brain tissues of Corvus corone, Melanitta fusca, Aquila heliaca, Aquila pomarina, Buteo rufinus, Accipiter nisus, Strix aluco and N. caninum in brain tissues of Larus genei, Corvus corone, Melanitta fusca, Anas clypeata, Perdix perdix, Aquila heliaca, Buteo rufinus in the world. This also is the first molecular diagnostic investigation of T. gondii and N. caninum in brain tissues of wild birds in Turkey.
A Snapshot Avian Surveillance Reveals West Nile Virus and Evidence of Wild Birds Participating in Toscana Virus Circulation
(Mary Ann Liebert, inc, 2017) Hacioglu, Sabri; Dincer, Ender; Isler, Cafer Tayer; Karapinar, Zeynep; Ataseven, Veysel Soydal; Ozkul, Aykut; Ergunay, Koray
Introduction: Birds are involved in the epidemiology of several vector-borne viruses, as amplification hosts for viruses, dissemination vehicles for the vectors, and sources of emerging strains in cross-species transmission. Turkey provides diverse habitats for a variety of wild birds and is located along major bird migration routes. This study was undertaken to provide a cross-sectional screening of avian specimens for a spectrum of vector-borne viruses. Materials and Methods: The specimens were collected in Hatay province, in the Mediterranean coast of the Anatolian peninsula, located in the convergence zone of the known migration routes. Generic PCR assays were used for the detection of members of Nairovirus, Flavivirus, and Phlebovirus genera of Flaviviridae and Bunyaviridae families. The circulating viruses were characterized via sequencing and selected specimens were inoculated onto Vero cell lines for virus isolation. Results and Discussion: Specimens from 72 wild birds belonging in 8 orders and 14 species were collected. A total of 158 specimens that comprise 32 sera (20.3%) from 7 species and 126 tissues (79.7%) from 14 species were screened. Eight specimens (8/158, 5%), obtained from 4 individuals (4/72, 5.5%), were positive. West Nile virus (WNV) lineage 1 sequences were characterized in the spleen, heart, and kidney tissues from a lesser spotted eagle (Clanga pomarina), which distinctly clustered from sequences previously identified in Turkey. Toscana virus (TOSV) genotype A and B sequences were identified in brain and kidney tissues from a greater flamingo (Phoenicopterus roseus), a great white pelican (Pelecanus onocrotalus), and a black stork (Ciconia nigra), without successful virus isolation. Partial amino acid sequences of the viral nucleocapsid protein revealed previously unreported substitutions. This study documents the involvement of avians in WNV dispersion in Anatolia as well in TOSV life cycle.