Browsing by Author "Kansu, Gulay"

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Evaluation of the Chitosan-Coating Effectiveness on a Dental Titanium Alloy in Terms of Microbial and Fibroblastic Attachment and the Effect of Aging
(inst Za Kovinske Materiale I in Tehnologie, 2015) Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Gungor, Serap; Evis, Zafer; Uyar, Pembegul; Akca, Gulcin; Kansu, Gulay
The aim of this study was to obtain a biocompatible and antimicrobial implant surface by coating Ti6Al4V with chitosan which can be used to create a smooth transmucosal region for a faster and better wound healing and an increased bioactivity. Ti6Al4V plates were first abraded and ultrasonically cleaned and then coated with chitosan. In order to simulate the conditions of an oral environment, a group of coated plates were treated in a thermocycle apparatus. The coatings were evaluated with SEM, EDS, XRD and FTIR spectroscopy. The fibroblastic cell behavior was determined using HGF-1 cells. P. gingivalis was used to assess the effectiveness of chitosan as an antimicrobial coating. It can be said that the Ti6Al4V plates were successfully coated with chitosan, indicated by the presence of the C, H and O elements in the EDS results. There were no significant differences between the XRD patterns of the coated and uncoated plates; however, the characteristic bands of chitosan were observed in the FTIR patterns of both the coated and aged samples. The fibroblast-cell attachment and proliferation were enhanced while the bacterial proliferation was inhibited by the chitosan coating. Chitosan was shown to be a biologically useful material that can be used as the coating material for transmucosal regions of dental implants.
Investigation of Surface Structure and Biocompatibility of Chitosan-Coated Zirconia and Alumina Dental Abutments
(Wiley, 2018) Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Koc, Serap Gungor; Evis, Zafer; Arpaci, Pembegul Uyar; Kansu, Gulay
Background: For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2O3) and zirconia (ZrO2) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues. Objective: The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces. Materials and Methods: The substrates were coated via solution casting technique. Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment. To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used. The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis. The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium salt assay. Results: It was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating. The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces. Conclusion: Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.