Browsing by Author "Karakurt, Emin"

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Comparison of Classical and Atypical Forms of Ovine Pulmonary Adenocarcinomas Using Immunohistochemical and Molecular Techniques
(University Agriculture, Fac Veterinary Science, 2025) Karakurt, Emin; Coskun, Nuvit; Keles, Omer Faruk; Beytut, Enver; Yaman, Turan; Uysal, Ayfer Yildiz; Cicek, Haci Ahmet
This study aimed to evaluate the classical and atypical forms of ovine pulmonary adenocarcinomas (OPA) by immunohistochemical and molecular methods. OPA forms were compared among themselves in terms of proliferating cell nuclear antigen (PCNA), p53 tumour suppressor gene, carcinoembryonic antigen (CEA) and vascular endothelial growth factor-A (VEGF-A) expressions. Avidin-biotin-peroxidase method was used for immunohistochemistry. Half of the cases were classified as classical form and other six were determined to be atypical form. All of the OPA forms had positive reactions for PCNA, p53, CEA and VEGF-A immunoreactivity. However, immunohistochemical and statistical analyses indicated no significant difference between the classical and atypical forms of OPA. In addition, Jaagsiekte sheep retrovirus nucleic acid detection was investigated using reverse transcription polymerase chain reaction (RT-PCR). Nucleic acids from paraffinated tissue blocks were isolated using a phenol/chloroform based method. Reverse transcription and polymerase chain reaction was performed using 2X One-step RT-PCR kit (Hibrigen, Turkiye, Catalogue Number: MG-OSPM-01). Five of the paraffin-embedded tissue samples (5/12) tested positive. Positive amplicons were sequenced and sequence data revealed that all isolates were type 2 exogenous Jaagsiekte sheep retrovirus (exJSRV), and positive samples contained minor amino acid changes among themselves. In conclusion, immunohistochemical parameters revealed the absence of significant difference between atypical and classical OPAs in terms of cancer dynamics, such as proliferation index, metastasis, angiogenesis capacity, and escape from apoptosis, and molecular methods also supported these findings.
Evaluation of the Relationship Between Inflammatory Reaction and Interleukins in Ovine Pulmonary Adenocarcinomas
(Urmia Univ, 2023) Karakurt, Emin; Coskun, Nuvit; Beytut, Enver; Keles, Omer Faruk; Dag, Serpil; Yilmaz, Volkan; Kurtbas, Emre
In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 -2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 -2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor -associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.(c) 2023 Urmia University. All rights reserved.