Browsing by Author "Kaya, Mehmet Salih"

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Antidiabetic Properties of Lyophilized Extract of Acorn (Quercus Brantii Lindl.) on Experimentally Stz-Induced Diabetic Rats
(Elsevier Ireland Ltd, 2015) Dogan, Abdulahad; Celik, Ismail; Kaya, Mehmet Salih
Ethnopharmacological relevance: Acorn obtained from the Quercus brantii Lindl. (QB) tree has been used in Turkish folk medicine. Some studies have reported as an antioxidant, antimicrobial, anti-inflammatory, gastroprotective, antitumoural of QB properties previously, however, its effect on the management of type diabetic 2 and oxidative stress complications is still unexplored. The aims of our study were the evaluation of the protective effect and antioxidant role of acorn lyophilized seed plant extract against STZ-induced diabetic complications as oxidative stress, hepatotoxicity and nephropathy, lipidemia and serum biomarkers of diabetes (SBD). Materials and methods: In-vivo studies were performed on STZ-induced diabetic rats Experiment was designed as I [Normal Control (NC)], II [Diabetes mellitus (DM)], III [DM+Acarbose (20 mg/kg b.w) (DM+AC-20)], IV [DM+ QB (100 mg/kg b.w) (DM+QB-100)], V [DM+QB (250 mg/kg b.w) (DM+ QB-250)] and VI [DM+QB (500 mg/kg b.w) (DM+QB-500)] groups. Results: This study showed that the biochemical analysis showed a considerable increase in the HRDB, DB, LP, MDA and fluctuated ADSC in the II group as compared to that of control group whereas, AC and the plant lyophilized seed plant extract supplementations diet restored the STZ-induced diabetic complications towards the control. alpha-glycosidase activity in DM group showed statistically significant increase with respect to control group in small intestine. Moreover, in accordance with the effects of seed extract; in diabetic rat groups to whom acorn seed extract and acarbose were given, the levels of almost all the concerned parameters were reached to the ones measured at control group. As a result, it was concluded that acorn seed extract had certain healing effects on many complications caused by diabetes. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
The Antioxidant Role of Sideritis Caesarea Infusion Against Tca Toxicity in Rats
(Cambridge Univ Press, 2011) Celik, Ismail; Kaya, Mehmet Salih
Sideritis caesarea (SC) Duman, Aytac&Baser is a member of the Lamiaceae family. The present study was designed to investigate the antioxidant properties of the aerial parts of island green tea SC against TCA effects in rats. Biomarkers selected for monitoring antioxidant capacity were the activities of glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase, catalase (CAT), GSH level and malondialdehyde (MDA) content in various organs of rats. Three experimental groups, A (untreated - control), B (only TCA-treated) and C (TCA + SC-treated), were studied. At the end of the 50 d experiment, the MDA content in tissues increased significantly in group B, whereas no significant changes were observed in group C as compared with that of the control group. Antioxidant enzyme activities such as SOD and CAT increased significantly in the brain, liver and kidneys of group B but decreased significantly in group C as compared with group B. The GSH level and GR activity increased significantly in the brain and liver of group C as compared with the control and TCA-exposed rats. Hence, the study reveals that the constituents present in SC impart protection against chemical-induced oxidative injury that may result in the development of cancer.
Çörek Otu (Nigella Sativa) Tohumunun İnsan Hücresel Bağışıklık Sisteminin Cd3+, Cd4+, Cd8+ Hücreleri ve Toplam Lökosit Sayısı Üzerine Etkileri
(2003) Kaya, Mehmet Salih; Kara, Mehmet; Özbek, Hanefi
Amaç: Çörek otu (Nigella sativä) tohumunun insanlarda hücresel bağışıklık sisteminin T lenfosit alt grupları ve toplam lökosit sayısı üzerine etkisinin araştırılması. Yöntem: Çalışma, yaş ortalaması 24 olan 30 gönüllü erkek üzerinde yapıldı. Uygulama öncesinde her bir gönüllüden EDTA'lı standart kan sayım tüplerine 2 ml venöz kan örneği alınarak Coulter Epics XL Flow Sitometri cihazında CDS+ (olgun T lenfosit), CD4+ (yardımcı T lenfosit) ve CD8+(süpresör-sitotoksik T lenfosit) hücreleri, Coulter STKS cihazında ise toplam lökosit sayımı yapıldı ve sonuçlar kontrol olarak kullanıldı. Deneklere 4 hafta süresince her gün 30 mg/kg çörek otu tohumu oral yoldan verildi ve 4. hafta sonunda tekrar kan örnekleri alınarak analiz edildi. Bulgular: CD3+ ve total lökosit değerlerinde istatistiksel açıdan anlamlı bir artış olduğu (sırasıyla P<0.01 ve P<0.001), diğer parametrelerin de arttığı ancak, bu artışın anlamsız (P>0.05) olduğu gözlendi. Sonuç: Çörek otu tohumunun insan bağışıklık sistemini güçlendirebileceği düşünüldü.
Evaluation of Neurotoxic and Immunotoxic Effects of Trichloroacetic Acid on Rats
(Sage Publications inc, 2010) Celik, Ismail; Isik, Ismail; Kaya, Mehmet Salih
This study was carried out to investigate the neurotoxic and immunotoxic effects of trichloroacetic acid (TCA) on rats at subchronic exposure. The neurotoxic effects of TCA were evaluated by measuring the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Biomarkers selected for immunotoxic monitoring were the activities of adenosine deaminase (ADA) and myeloperoxidase (MPO) in various tissues of rats exposed to 2000 parts per million (ppm) dosage of TCA for 52 days. Results showed that the administrations of TCA decreased BChE activities in heart and lungs tissue of the rats treated with TCA. With regard to the immunotoxic effects, ADA activity significantly decreased in the heart, lungs and spleen whereas MPO activity increased after subchronic exposure with 2000 ppm dosage in all of the tissues except for heart tissue of rats compared with controls. The observations presented led us to conclude that the administration of TCA at subchronic was decreased BChE and ADA activities whereas increased MPO activity in various tissues of rats. This may reflect the potential role of these parameters as useful biomarkers for toxicity of TCA.
Evaluation of the Effects of Intermittent Fasting Diet and Capsaicin Treatment on the Survival of Laser Damaged Drg Neurons in Experimental Obesity Model
(Wiley, 2022) Seker, Ayse; Kaya, Mehmet Salih; Ustun, Ramazan
High Fat Diet Induces Anti-Inflammatory Effect in Experimental Colitis
(Natl inst Science Communication-niscair, 2023) Emlik, Huseyin; Bayiroglu, Fahri; Mis, Leyla; Kaya, Mehmet Salih; Gunbatar, Nizamettin
The prevalence of ulcerative colitis, one of the inflammatory bowel diseases, has been increasing worldwide. Though fatty diet leads to development of colitis, only little is known about the separate and combined effects of different fat compositions and intermittent fasting, and the effects of preventative dietary changes on experimental colitis. In this study, we investigated the effect of feeding type and frequency on the inflammation ulcerative colitis. Five groups, each comprising 7 male Wistar albino rats, were formed viz. Colitis (as a control), High sucrose diet + Colitis, High sucrose diet + Intermittent fasting + Colitis, High fat diet + Colitis, High fat diet + Intermittent fasting + Colitis. The intermittent fasting group (experimental group) was not fed for 2 days (non-consecutive) in a week (except water), otherwise fed with ad libitum. Following the 7-wk feeding application, the rats were treated with intrarectal administration of 4% acetic acid (pH 2.4). After the rats were sacrificed, the levels of interleukin-10, insulin, C-reactive protein, leptin, tumor necrosis factor-alpha, interleukin-6, insulin-like growth factor-1 and adiponectin were analyzed from the blood samples. Analyzes of results revealed a statistically significant decrease in interleukin-10, C-reactive protein, tumor necrosis factor-alpha, interleukin-6 and adiponectin levels in High sucrose diet + Intermittent fasting + Colitis and High fat diet + Intermittent fasting + Colitis groups (P<0.05). According to these findings, the amount of fat and fatty acid composition in the fatty diet may have a protective effect against the development of colitis. Intermittent fasting further enhances this protective effect by lowering proinflammatory cytokine levels and reducing systemic inflammation in the body.
In Case of Obesity, Longevity-Related Mechanisms Lead To Anti-Inflammation
(Springer, 2014) Kaya, Mehmet Salih; Bayiroglu, Fahri; Mis, Leyla; Kilinc, Dide; Comba, Bahat
The exact mechanisms which contribute to longevity have not been figured out yet. Our aim was to find out a common way for prompting longevity by bringing together the well-known applications such as food restriction, exercise, and probiotic supplementing in an experimental obesity model. Experimental obesity was promoted in a total of 32 young (2 months old) and 32 aged (16 months old) male Wistar albino rats through 8-week cafeteria diet (salami, chocolate, chips, and biscuits). Old and young animals were divided into groups each consisting of eight animals and also divided into four subgroups as obese control, obese food restriction, obese probiotic-fed and obese exercise groups. Probiotic group diet contained 0.05 %w/total diet inactive and lyophilized Lactobacillus casei str. Shirota. The exercise group was subjected to treadmill running 1 h/day, at 21 m/min and at an uphill incline of 15 % for 5 days a week. Food restriction group was formed by giving 40 % less food than the others. The control group was fed regular pellet feed ad libitum. This program was continued for 16 weeks. Blood samples from all the groups were analyzed for fasting glucose, insulin, IGF-1, insulin-like growth factor binding protein 3 (IGFBP-3), interleukin (IL)-6, IL-12, malondialdehyde (MDA), fT3, TT3, fT4, TT4, and liver tissue MDA levels were measured. All applications showed anti-inflammatory effects through the observed changes in the levels of IGFBP-3, IL-6, and IL-12 in the young and old obese rats. While the interventions normally contribute to longevity by recruiting different action mechanisms, anti-inflammatory effect is the only mode of action for all the applications in the obesity model.
Protective Effects of Silymarin on Methotrexate-Induced Damages in Rat Testes
(Univ Sao Paulo, Conjunto Quimicas, 2018) Yaman, Turan; Uyar, Ahmet; Kaya, Mehmet Salih; Keles, Omer Faruk; Uslu, Baris Atalay; Yener, Zabit
The present study aimed to investigate the protective effects of silymarin (SMN), an antioxidant, on methotrexate (MTX)-induced damage in rat testes. Thirty-two Wistar albino rats were divided into four groups (n = 8): control, MTX (20 mg/kg, i.p. on days 1 and 5), SMN (200 mg/kg, orally), and MTX + SMN (20 mg/kg, i.p. on days 1 and 5 and SMN 200 mg/kg orally) groups. At the end of the 6-week trial period, histopathological, immunohistochemical, biochemical, and spermatological analyses were performed on testes tissues. Histopathologically, MTX-induced damage, including depletion of germ cell and loos of spermatozoa, was significantly improved with SMN treatment. Immunohistochemically, the immunoreactivity of glutathione peroxidase 1 (GPx1) and manganese superoxide dismutase 2 (SOD2) were detected more intensely in the MTX + SMN group than in the MTX group. Biochemical examinations revealed that SMN supplementation decreased the lipid peroxidation and increased enzymatic antioxidants in the SMN-treated rats. Spermatologically, significant differences were found in the density, motility, dead-to-live sperm ratio, and abnormal sperm rate in the MTX + SMN group compared to the MTX group. In conclusion, SMN seems to have protective effects as an antioxidant against MTX-induced damage in rat testes.
The Effects of Food Restriction, Exercise and Probiotic Bacteria Administration on Some Parameters Related With Longevity in Experimental Obese Rat Model
(2009) Kaya, Mehmet Salih; Bayıroğlu, Fahri
Bu çalışmada, gıda kısıtlaması, probiyotik bakteri ve egzersiz uygulamalarının genç ve yaşlı obez sıçanlarda, uzun ve yaşlılık patolojilerinden uzak yaşama ile ilgili olduğu düşünülen bazı parametrelerin karşılaştırmalı olarak gözlenmesi amaçlanmıştır. Bu amaçla, 32 adet genç (2 aylık) ve 32 adet yaşlı (16 aylık) erkek Wistar albino sıçanda 8 haftalık kafeterya diyeti (salam, çikolata, cips, bisküvi) uygulaması ile diyete bağlı deneysel obezite oluşturuldu. Obezite oluşturulduktan sonra normal pelet yem ile beslemeye geçildi. Genç ve yaşlı hayvanlar her bir grupta 8 tane olacak şekilde kendi içerisinde obez kontrol, obez gıda kısıtlamalı, obez probiyotik bakteri uygulamalı ve obez egzersiz grubu olmak üzere dörder alt gruba ayrıldı. Obez gıda kısıtlamalı gruba kontrollerine göre %40 daha az gıda verilerek kısıtlama oluşturuldu. Obez probiyotik grubun yemlerine %0.05 oranında inaktive ve lyofilize edilmiş Lactobacillus casei str. Shirota bakterisi ilave edildi. Obez egzersiz grubu ise haftada 5 gün 21m/dak hız ve %15 eğimli motorlu koşu bandında günde 1 saat koşturuldu. 16 haftalık uygulamalar sonrasında gruplardan alınan kan örneklerinden AKŞ, İnsülin, IGF-1, IGFBP-3, IL-6, IL-12, MDA, fT3, TT3, fT4 ve TT4 değerleri analiz edilirken, karaciğer dokusundan ise MDA analizi gerçekleştirildi. Yapılan analizler sonucunda genç ve yaşlı obezlerin IGFBP-3, IL-6 ve IL-12 seviyeleri üzerine bütün uygulamaların antiinflamatuar etkili olduğu gözlenmiştir. Bu bulgular sonucunda obezite modelinde her üç uygulamanın da antiinflamatuar etki göstermesi, uzun yaşamayı olumsuz etkileyen kronik inflamasyon tehdidi karşısında organizmada öncelikle bunun ortadan kaldırılmasına dönük ortak mekanizmanın kullanıldığı ortaya konulmuştur. Bu da uzun yaşama ile ilgili parametreleri olumlu etkileyen uygulamaların, canlı organizmanın fizyolojik durumuna bağlı olarak etkinliklerini farklı mekanizmalar üzerinden gösterebildiğini ortaya koymaktadır. Sonuç olarak obez bireylerde uzun yaşama ile ilgili en önemli müdahalenin organizmada genel inflamasyon olgusunu azaltmak yoluyla olabileceği söylenebilir .