Browsing by Author "Kayikci, Caner"

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Effect of Low- and High-Dose Methotrexate on Wound Healing in Rats
(Acta Cirurgica Brasileira, 2025) Karasu, Abdullah; Kuscu, Yagmur; Kayikci, Caner; Yildirim, Serkan; Kuscu, Oguzhan; Kiliclioglu, Metin
Purpose: To investigate the effect of intraperitoneal treatment with low- and high-dose methotrexate (MTX) on wound healing in rats. Methods: The study sample consisted of 54 healthy rats. Under aseptic conditions, skin wounds were created with two circular fullthickness punch tools, 10 mm in diameter, one on the right and the other one on the left of the dorsal vertebral line. The rats were randomly assigned to one of three main treatment groups. On the 0th day (2 hours before wound creation), 7th day, and 14th day, the control group received 0.3-mL saline, the low-MTX group received 3 mg/kg MTX, and the high-MTX group received 30 mg/kg MTX, all administered intraperitoneally. The wounds were evaluated seven, 14, and 21 days after injury through morphometrical, biochemical, histopathological, and immunohistochemical analyses. Results: MTX dose-dependently decreased the degree of inflammation and angiogenesis, tissue hydroxyproline level, and HSP70 and tumor necrosis factor-alpha expression in the early phase of wound healing. It also suppressed epithelialization and collagen 1 expression throughout the wound-healing process. Conclusion: The wounds treated with high-dose of MTX had statistically delayed wound closure on days 7, 14 and 21 compared to the saline group, while wounds treated with low-dose of MTX only had statistically delayed wound closure on day 14. In addition, weight loss was observed in rats treated with high-dose MTX, which was thought to reflect its toxicity. The dose-dependent adverse effect of MTX on wound healing may be due to its antiproliferative, antifibrotic, anti-inflammatory, and antiangiogenic effects.
Effects of Silymarin on Immunohistochemical Bax and 8-Ohdg Expression, Biochemical Markers and Sperm Parameters in an Experimental Varicocele Model in Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2025) Belhan, Saadet; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Yildirim, Serkan
In this study, the effects of silymarin on immunohistochemical Bcl-2-associatedXprotein (Bax)and8-hydroxy-2'- methoate (DMT e cide is e of t che cals used to otectdeoxyguanosinesomeagriural(8-OHdG) areas m expression, harmful o nisms. biochemical DMT markers and sperm parameters were investigated with an sidues elea ed directly or ind rectly to the envi onm nt experimentally induced varicocelemodelin rats.Thestudy use serious problems in nature DMT resid es mixed wi hthe quat c environmen adversely affect aquat c organisms a d was conducted on 36 Wistar albino rats. The distribution of rats within the group was made in an equal number. Rats in the control group were administered physiological saline daily is effect is carr ed to humans through the food chain. In thisvia oral gavage. In the sham group, an incision was made on udy, oxdative s ress respon es nd ced by DMT pe tic de inontastacusleptodacylus were nvestigated.Forthispurpose, the midline and the renal vein (located on the left) was made xidatvisible.ve A stress probe and was placed antioxidant on thisparametevein. sThe probe Thiobarbituric was coiled acid with the vein but not ligated. In the silymarin group, silymarin active s bstances (TBARS) Glutathione (GSH) Superoxidewas administered by oral gavage at adoseof 75 mg/kg 3 smu ase (SOD) c ta ase CAT) and lutathi ne pe oxidase times a week for 8 weeks. Ligation was performed on rats in PX) caused by dimethoate (DMT) pesticde in P.leptodactylus the varicocele group, unlike the sham group. Varicocele was created inthe varicocele+silymarin groups (50 mg/kg, 75 mg/ 17.5, 35, and 70 mg i-1 concentr tions at 24 and 96 h urs ere investigated Results were determined using EiISA kits. kg). Silymarin application was started 8 weeks after varicocele induction and was applied 3 days a week for 8 weeks. After the analysis, it was seen that sperm parameters were negatively ere obse ved between decreases n CAT and GPx activi ies and affected in the varicocele group. Additionally, severe caspase cr ases in TBARS leve s SPSS 24 0 packa e program one-way 3, 8-OHdG and Bax expressions were detected. Silymarin NOVA (Duncan 05) was used n the evaluation of bio hem cal administration reduced the intensity of expression and had nalyzes As a res lt,t was determined that DMT caused oxidative positive effects on spermatology. These positive effects were even more pronounced with the 75 mg dose. Based on the ress formation in P leptodac ylus and caused changes n enzyme results obtained, silymarin may have the potential to reduce both clinical and pathological symptoms in varicocele cases. tivities.
Investigation of the Effect of Silymarin on Oxidative Dna Damage and Inflammatory Markers in Ischemia/Reperfusion Injury Following Experimental Testicular Torsion/Detorsion in Rats
(Tubitak Scientific & Technological Research Council Turkey, 2021) Belhan, Saadet; Yildirim, Serkan; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Kuscu, Yagmur
The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats (10-12 weeks of age, weighing 280-300 g) were randomly divided into five groups. Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin (250 mg/kg) group; and torsion 3 h / detorsion 24 h + silymarin (250 mg/kg) group. In the study, 720 degrees torsion was applied to the left testicle. At the end of the study, blood was collected from the rats, and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha (Tnf-alpha) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities decreased, while malondialdehyde (MDA) levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.
Serum Retinol, Alpha-Tocopherol, Cholecalciferol, and Some Mineral Levels in Ruminants With Congenital Digestive and Urogenital System Anomalies1
(Revista Pesquisa veterinaria Brasileira, 2024) Karasu, Abdullah; Genccelep, Musa; Kayikci, Caner; Kuscu, Yagmur
This study aimed to investigate the relationship between serum vitamin and mineral levels and congenital defects in digestive and urogenital system anomalies in calves, lambs, and kids. The study material consisted of 13 calves, 15 lambs, 10 kids clinically and radiologically diagnosed with congenital digestive and urogenital system anomalies and 10 newborn clinically healthy calves, 10 lambs, and 10 kids. Congenital defects were diagnosed by clinical and radiological examination. Blood samples were collected from all animals, and sera were extracted for biochemical analysis. Vitamins A, D, and E, calcium, phosphorus, sodium, potassium, chlorine, magnesium, copper, iron, zinc, selenium, and manganese levels were measured in serum samples. Penile urethral diverticulum in kids, atresia ani, atresia ani with vaginal fistula in lambs, and atresia ani and atresia coli defects in calves were determined. Copper levels were higher, and zinc levels were lower in kids with penile urethral diverticulum compared to the control group. Vitamin A levels were lower in lambs with digestive system anomalies compared to the control group. Meanwhile, copper levels were higher in lambs with digestive system anomalies. Vitamin A and D levels were lower in calves with digestive system anomalies compared to the control group. There was no difference in the levels of the other parameters compared to the control group. In conclusion, insufficient serum vitamin A levels may play a role in the etiopathogenesis of congenital intestinal atresia in calves and lambs. Therefore, we believe that parenteral vitamin A administration to the mother, especially in the last trimester of pregnancy in regions with continental climates and poor green vegetation, would be beneficial. Further research should be conducted to determine the role of vitamin A in the etiopathogenesis of congenital atresia ani and coli.