Effects of Silymarin on Immunohistochemical Bax and 8-Ohdg Expression, Biochemical Markers and Sperm Parameters in an Experimental Varicocele Model in Rats

Abstract

In this study, the effects of silymarin on immunohistochemical Bcl-2-associatedXprotein (Bax)and8-hydroxy-2'- methoate (DMT e cide is e of t che cals used to otectdeoxyguanosinesomeagriural(8-OHdG) areas m expression, harmful o nisms. biochemical DMT markers and sperm parameters were investigated with an sidues elea ed directly or ind rectly to the envi onm nt experimentally induced varicocelemodelin rats.Thestudy use serious problems in nature DMT resid es mixed wi hthe quat c environmen adversely affect aquat c organisms a d was conducted on 36 Wistar albino rats. The distribution of rats within the group was made in an equal number. Rats in the control group were administered physiological saline daily is effect is carr ed to humans through the food chain. In thisvia oral gavage. In the sham group, an incision was made on udy, oxdative s ress respon es nd ced by DMT pe tic de inontastacusleptodacylus were nvestigated.Forthispurpose, the midline and the renal vein (located on the left) was made xidatvisible.ve A stress probe and was placed antioxidant on thisparametevein. sThe probe Thiobarbituric was coiled acid with the vein but not ligated. In the silymarin group, silymarin active s bstances (TBARS) Glutathione (GSH) Superoxidewas administered by oral gavage at adoseof 75 mg/kg 3 smu ase (SOD) c ta ase CAT) and lutathi ne pe oxidase times a week for 8 weeks. Ligation was performed on rats in PX) caused by dimethoate (DMT) pesticde in P.leptodactylus the varicocele group, unlike the sham group. Varicocele was created inthe varicocele+silymarin groups (50 mg/kg, 75 mg/ 17.5, 35, and 70 mg i-1 concentr tions at 24 and 96 h urs ere investigated Results were determined using EiISA kits. kg). Silymarin application was started 8 weeks after varicocele induction and was applied 3 days a week for 8 weeks. After the analysis, it was seen that sperm parameters were negatively ere obse ved between decreases n CAT and GPx activi ies and affected in the varicocele group. Additionally, severe caspase cr ases in TBARS leve s SPSS 24 0 packa e program one-way 3, 8-OHdG and Bax expressions were detected. Silymarin NOVA (Duncan 05) was used n the evaluation of bio hem cal administration reduced the intensity of expression and had nalyzes As a res lt,t was determined that DMT caused oxidative positive effects on spermatology. These positive effects were even more pronounced with the 75 mg dose. Based on the ress formation in P leptodac ylus and caused changes n enzyme results obtained, silymarin may have the potential to reduce both clinical and pathological symptoms in varicocele cases. tivities.