Browsing by Author "Keles, O. F."

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Exploring the Protective Effects of Chinar (Platanus Orientalis L.) Leaf on Testes Damage Induced by Ethanol in Rat Model
(Pakistan Agricultural Scientists Forum, 2024) Yaman, T.; Dogan, A.; Keles, O. F.; Donmez, F.; Battal, A.; Anuk, O. O.
The aim of this study was to investigate the protective effects of an infusion obtained from dried Chinar (Platanus orientalis L.; PO) leaves against ethanol (EtOH)-induced damage in rat testes. Thirty male Wistar rats were randomly and equally divided into 5 groups, as the Control, EtOH 20%, EtOH 20% + Silymarin (10 mg/kg/day), EtOH 20% + PO20 mg/mL infusion, and EtOH 20% + PO -60 mg/mL infusion. Histopathologically, treatment with PO -60 leaf infusion resulted in improvements in EtOH-induced damage in the testes by inhibiting the depletion of germ cells and loss of spermatozoa. Immunohistochemically, an increase in the expression of endothelial nitric oxide synthase (eNOS) and caspase-3 was observed in the EtOH group. Treatment with the PO extract markedly reduced the EtOH-induced expression of eNOS and caspase-3 in the EtOH 20% + PO -60 group. According to the biochemical results, both of the PO infusion treatments caused a decrease in the malondialdehyde levels compared to the EtOH group. On the other hand, the glutathione contents in the treatment groups were significantly higher than ethanol group. Fluctuations in antioxidant defense enzyme activities were determined. PO leaf might have a protective role against EtOH-induced testicular damage in rats by inducing apoptosis via the upregulation of eNOS expression and preventing lipid peroxidation.
Protective Effect of Astaxanthin in the Lung Injury Caused by Ischemia Reperfusion of the Lower Extremities
(Pakistan Agricultural Scientists Forum, 2019) Akkoyun, H. T.; Uyar, A.; Bengu, A. S.; Akkoyun, M. Bayramoglu; Arihan, O.; Keles, O. F.
Pathological and biochemical alterations due to lower extremity (I/R) damage and protective effects of astaxanthine (AST) were investigated. Rats were divided into four groups. GI-Sham group (n=7):Anesthesia without (I/R)(2hours);GII-I/R (n=7) : 2 hours of ischemia and 2 hours of reperfusion under anesthesia; Group III-AST(n=7): Rats were subchronically orally administered for 7 days at 125 mg/kg astaxanthin (AST) and then anesthetized (2hours) without ischemia; GIV-I/R+AST (n=7) : 7 days prior to ischemia rats were subchronically orally administered 125 mg/kg astaxanthin (AST) and then 2 hours of ischemia and reperfusion under anesthesia; Then lung tissues were investigated for MDA,GSH and histopathology. An increase in MDA and a decrease in GSH was observed I/R administered group compared to control. Histopathological evaluations showed intense congestion in pulmonary veins and alveolar septum and partial alveolar macrophage and erythrocyte accumulation and edema was observed in lumens of some bronchioles and alveoli in the second and fourth group compared control. Second group (3.41) damage score had high significance compared to control (p <= 0.001). Fourth group damage score (0.92) was indifferent from control but significantly different from I/R group (p <= 0.001). As a result; The protective effect of AST has been demonstrated by biochemical, histopathological and immunohistochemical effects.