Browsing by Author "Kemik, Ahu Sarbay"

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Derin Ven Trombozu Olan ve Olmayan Hastalarda D-dimer ve Tüm Hemostatik Faktörlerin Düzeyleri
(2010) Kemik, Ahu Sarbay; Kotan, Çetin; Kemik, Özgür; Tüzün, Sefa; Sumer, Aziz; Purisa, Sevim
Amaç: Gelişen tanı ve tedavi yöntemlerine rağmen, derin ven trombozu (DVT) hala önemli bir morbidite ve mortalite nedeni olmaya devam etmektedir. DVT sık karşılaşılan bir hastalık olup, Amerika Birleşik Devletlerinde her yıl 250 000’den fazla kişiyi etkilemektedir. DVT tanısında mevcut tanı araçlarının kısıtlılıklarından dolayı plazma D-dimer gibi laboratuar ölçümlerine son dönemlerde ilgi artmıştır. Bu nedenle biz, derin ven trombozlu hastalardaD-dimer düzeylerini ve tüm hemostatik faktörleri incelemeyi amaçladık. Gereç ve Yöntem: Derin ven trombozlu 120 hastanın ve derin ven trombozu olmayan 115 hastanın, D-dimer düzeylerini ve Von Willebrand faktör düzeylerini enzim bağlı immunosorbent assay (ELISA) yöntemi ile inceledik. Kolesterol, kreatinin, INR, faktör VIII: C oranı ve aPTT ölçümleri standard metod ile, fibrinojen ölçümü ise Clauss metodu ile ölçüldü. Bulgular: Yaş, vücut kitle indeksi (VKİ), aPTT, INR ve total kolesterol bakımından derin ven trombozlu hastalarla derin ven trombozu olmayan hastalar karşılaştırıldığında, anlamlı farklılık bulunamadı (p > 0,05). Plasma D-dimer düzeyleri, kreatinin, faktör VIII: C ve Von Willebrand faktör düzeylerini derin ven trombozlu hastalarda, derin ven trombozu olmayan hastalara göre anlamlı olarak yüksek bulduk (sırasıyla, p<0,001, p<0,01). Diğer taraftan, fibrinojen düzeylerini, derin ven trombozlu hastalarda düşük bulduk (p< 0,01). Sonuç: Derin ven trombozunun teşhis ve tedavisinde D-dimer düzeylerinin ve hemostatik faktörlerin önemli rol oynadığı kanısındayız.
Dickkopf-1 Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2014) Kaba, Mehmet; Pirincci, Necip; Benli, Erdal; Gecit, Ilhan; Gunes, Mustafa; Yuksel, Mehmet Bilgehan; Kemik, Ahu Sarbay
Background: Evidence indicates that Dickkopf-1 (DKK-1) levels may be a biomarker for cancer risk. The aim of this study was to assess DKK-1 and its correlation with clinic-pathological features in patients with bladder cancer. Materials and Methods: DKK-1 levels were determined in serum samples from 90 patients with bladder cancer before transurethral tumor resection. The concentrations of DKK-1 were determined by using enzyme linked immune-sorbent assay (ELISA). Results: Elevated preoperative DKK-1 levels were associated with tumor stage (p<0.001), grade (p<0.001) and histological grade (p<0.001). Conclusions: The results of our study demonstrated that the level of serum DKK-1 is correlated with both disease progression and increase in the tumor grade. Preoperative serum DKK-1 elevation may thus represent a novel marker for the determination of bladder cancer and the detection of patients with a likely poor clinical outcome.
Haptoglobin Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2012) Pirinicci, Necip; Gecit, Ilhan; Gunes, Mustafa; Kemik, Ahu Sarbay; Yuksel, Mehmet Bilgehan; Kaba, Mehmet; Aslan, Mehmet
Although alteration in the haptoglobin phenotype has been reported in patients with bladder cancer, serum haptoglobin levels have not been evaluated. We hypothesized that serum haptoglobin can be used as a biomarker. The aim of this study was to evaluate the expression of haptoglobin in bladder cancer and to determine the relationship with clinicopathological features. A total of 68 serum specimens obtained before surgery were used to investigate haptoglobin expression using the sandwich ELISA technique. Serum haptoglobin levels were higher in the patients with bladder cancer compared to healthy controls (p<0.0001). Additionally, the levels of haptoglobin protein increased with increasing tumor grades (p<0.001) and were significantly higher in patients with metastatic disease and the presence of lymphovascular involvement, lymph node metastases and increasing tumor burden (p<0.0001). This study suggests that elevated haptoglobin levels are associated with a higher stage, grade, and extent of distant metastasis and larger tumor size. Haptoglobin may therefore provide a useful diagnostic and treatment biomarker for patients with bladder cancer.
Human Vascular Adhesion Protein-1 (vap-1): Serum Levels for Hepatocellular Carcinoma in Non-Alcoholic and Alcoholic Fatty Liver Disease
(Biomed Central Ltd, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Itik, Veyis; Dulger, Ahmet Cumhur; Purisa, Sevim; Tuzun, Sefa
Background: The incidence of hepatocellular cancer in complicated alcoholic and non-alcoholic fatty liver diseases is on the rise in western countries as well in our country. Vascular adhesion protein-1 (VAP-1) levels have been presented as new marker. In our study protocol, we assessed the value of this serum protein, as a newly postulant biomarker for hepatocellular cancer in patients with a history of alcoholic and non-alcoholic fatty liver diseases. Methods: Pre-operative serum samples from 55 patients with hepatocellular cancer with a history of alcoholic and non-alcoholic fatty liver diseases and patients with cirrhosis were assessed by a quantitative sandwich ELISA using anti-VAP-1 mAbs. This technique is used to determine the levels of soluble VAP-1 (sVAP-1) in the serum. Results: sVAP-1 levels were evaluated in patients with hepatocellular cancer and liver cirrhosis. There was a significant difference in mean VAP-1 levels between groups. Serum VAP-1 levels were found higher in patients with hepatocellular cancer. Conclusion: These findings indicate that the serum level of sVAP-1 might be a beneficial marker of disease activity in chronic liver diseases.
Kolorektal Kanserli Hastalarda Doku İnhibitor Metalloproteinaz-3 Düzeylerinin Önemi
(2010) Tüzün, Sefa; Purisa, Sevim; Dülger, Ahmet Cumhur; Kemik, Özgür; Hasırcı, İsmail; Beğendik, Hüseyin; Kemik, Ahu Sarbay
Pek çok matriks metalloproteinaz düzeyleri, kolorektal kanserli hastalarda araştırılmış ve aralarında güçlü bir ilişki bulunmuştur. Ancak matriks metalloproteinazların doku inhibitörleri ile kanser arasında çok fazla araştırma yapılmamıştır. Biz bu çalışmamızda doku inhibitörü matriks metalloproteinaz-3 (TIMP-3) düzeylerini kolorektal kanserli hastalarda incelemeyi amaçladık. Çalışmamızda 100 kolorektal kanserli hastanın serum düzeyleri enzim bağlı immunosorbent ölçüm yöntemi ile analiz edildi. Kolorektal kanserli hastalarda serum TIMP-3 düzeyleri kontrollerimize göre, yüksek bulduk. Hastalarımızın serum CEA düzeyleri ile TIPM-3 düzeyleri arasında da pozitif korelasyon saptadık. Elde ettiğimiz sonuçlara göre, kolorektal kanserli hastalarda serum TIMP-3 düzeylerinin çok önemli bir prognostik markır olabileceğini düşünmekteyiz. Bizim bulduğumuz bu sonuçların, kanser araştırmalarına ışık tutacağını düşünmekteyiz
Levels of Matrix Metalloproteinase-1 and Tissue Inhibitors of Metalloproteinase-1 in Gastric Cancer
(Baishideng Publ Grp Co Ltd, 2011) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Dulger, Ahmet Cumhur; Adas, Mine; Begenik, Huseyin; Kotan, Cetin
AIM: To evaluate the levels of preoperative serum matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in gastric cancer. METHODS: One hundred gastric cancer patients who underwent gastrectomy were enrolled in this study. The serum concentrations of MMP-1 and TIMP-1 in these patients and in fifty healthy controls were determined using an enzyme-linked immunosorbent assay. RESULTS: Higher serum MMP-1 and TIMP-1 levels were observed in patients than in controls (P < 0.001). Serum MMP-1 and TIMP-1 levels were positively associated with morphological appearance, tumor size, depth of wall invasion, lymph node metastasis, liver metastasis, perineural invasion, and pathological stage. They were not significantly associated with age, gender, tumor location, or histological type. CONCLUSION: Increased MMP-1 and TIMP-1 were associated with gastric cancer. Although these markers are not good markers for diagnosis, these markers show in advanced gastric cancer. (C) 2011 Baishideng. All rights reserved.
The Levels of Mmp-2 İn Pancreatıc Juıce From Pancreatıc Adenocarcınoma Patients
(2010) Kemik, Ahu Sarbay; Kemik, Özgür; Dülger, A.cumhur; Adas, Mine; Purisa, Sevim; Tüzün, Sefa; Beğendik, Hüseyin
Pankreas kanseri için erken teşhis edilebilecek ve yüksek riskli hasta grubunda özel bir biomarkır yoktur. Pankreastan salınan proteinlerin bulunduğu zengin bir kaynak olan panreatik sıvı, pancreas kanserinin biyolojik keşfi için ideal bir kaynaktır. 10 pankreatik adenokarsinomalı hasta ve 10 sağlıklı bireyde pancreas suyu alınarak, MMP-2 düzeyleri enzim bağlı immunosorbent ölçüm yöntemi (ELISA) ile belirlendi. Pankreas salgısı MMP-2 düzeyleri, kontrol grubuna göre, pancreas kanserli hastalarda yüksek bulundu (p<0.001). MMP-2 düzeyleri pancreas kanserinin tanımlanmasında ve taramasında yarar sağlayacak, klinik uygulamaya konabilecek bir markırdır.
The Levels of the D-Dimer and All Haemostatic Factors in Patients With and Without Deep Venous Thrombosis
(Yerkure Tanitim & Yayincilik Hizmetleri A S, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Purisa, Sevim; Tuzun, Sefa; Kotan, Cetin
Objective: Although improved diagnostic tools and treatment approach, deep venous thrombosis is still an important cause of morbidity and mortality. Deep venous thrombosis is a common condition that affects more than 250 000 patients each year in the United States. In spite of limitations of the available diagnostic tools for detecting deep venous thrombosis, laboratory tests such as plasma D-dimer has been gained much interest recently. So, we aimed to investigate the D-dimer and all hemostatic factors in patients with deep vein thrombosis. Material and Methods: We investigated the levels of the D-dimer and Von Willebrand factor by enzyme linked immunosorbent assay (ELISA) in 120 patients with deep venous thrombosis and in 115 patients without deep venous thrombosis. We used standard methods for detecting the levels of the cholesterol, creatinin, INR, factor VIII: C ratio, and aPTT. We used Clauss method for detecting the fibrinogen levels. Results: We obtained important results in patients with deep venous thrombosis compared to in patients without deep vein thrombosis. Age, BMI, aPTT, INR, and total cholesterol levels were not found statistically differences in patients with deep venous thrombosis (p> 0,05). Plasma D-dimer, creatinin, factor VII: C, and Von Willebrand factor levels were found higher in patients with deep venous thrombosis than in patients without deep venous thrombosis (respectively, p<0,001, p<0,01). But, plasma fibrinogen levels were not found lower in patients with deep venous thrombosis (p<0,01). Conclusion: In conclusion, we suggested that the plasma D-dimer and hemostatic factor levels may play an important role in diagnosis and treatment of deep venous thrombosis.
Lipid Peroxidation and Transforming Growth Factor-Β1 Levels in Gastric Cancer at Pathologic Stages
(Galenos Publ House, 2012) Tuzun, Sefa; Yucel, Ahmet Fikret; Pergel, Ahmet; Kemik, Ahu Sarbay; Kemik, Ozgur
Objective: High levels of TGF-beta 1 and enhanced TGF-beta 1 receptor signaling are related to the pathology of gastric cancer. This effect is caused by oxidative stress and lipid peroxidation products. The aim of this study was to investigate the levels of TGF-beta 1 and lipid peroxidation products in gastric cancer patients and their correlation with pathologic stage. Material and Methods: Lipid peroxidation products and TGF-beta 1 levels were studied in the serum samples of 50 gastric cancer patients and 18 control subjects. Results: HNE-protein adducts and TGF-beta 1 levels were significantly higher in T2, T3 and T4 gastric cancers than in either the T1 stage or controls (p<0.001). Pathologic stage was correlated with TGF-beta 1 levels (r=0.702, p<0.05). Conclusion: These markers production may contribute to tumor angiogenesis and aid in the prognosis of the gastric cancer.
Plasma Human Neutrophil Proteins-1, And-3 Levels in Patients With Bladder Cancer
(Springer, 2013) Gunes, Mustafa; Gecit, Ilhan; Pirincci, Necip; Kemik, Ahu Sarbay; Purisa, Sevim; Ceylan, Kadir; Aslan, Mehmet
Human neutrophil proteins-1, -2, and -3 (HNPs -1, -2, and -3) are expressed in several tumor types. However, the role of HNPs 1-3 in human bladder cancer has not yet been determined. We investigated the association between the plasma levels of HNPs 1-3 and clinicopathological parameters in bladder cancer patients. The plasma levels of HNPs 1-3 were measured in 60 patients with bladder cancer and in 58 healthy controls. The plasma levels of HNPs 1-3 were determined by a solid-phase enzyme-linked immunosorbent assay (ELISA). Plasma samples were obtained before surgery. Plasma samples were permitted to clot and were then stored at -80 A degrees C until use. The levels of the HNPs increased from grade 1 to 4 tumors and this difference was statistically significant (p < 0.001). Additionally, plasma HNP levels were significantly higher in patients with metastatic bladder cancer and in patients with lymphovascular involvement, metastasis of the lymph nodes, and increased tumor burden (p < 0.001). The preoperative plasma levels of HNPs 1-3 paralleled the progression and pathological stages of the malignancies. This study suggests that HNPs 1-3 promote tumor invasion and are potential indicators of disease progression in patients with bladder cancer.
Preoperative Serum Placenta Growth Factor Level as a New Marker for Stage Ii or Iii Colorectal Cancer Patients
(Turkish Soc Gastroenterology, 2012) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Purisa, Sevim; Tukenmez, Baris; Tuzun, Sefa
Background/aims: We first reported in this study that serum placenta growth factor and carcinoembryonic antigen in combination were useful markers for selecting early-stage colorectal cancer patients. The aim of the present study was to determine whether serum placenta growth factor could provide carcinoembryonic antigen-independent prognostic information on patients undergoing curative surgery. Methods: Serum and tissue samples were collected from 158 patients with colorectal cancer and from 50 controls. Serum and tissue levels of placenta growth factor were measured by enzyme-linked immunosorbent assay. The serum placenta growth factor levels in colorectal cancer patients were compared with those in healthy controls, and we retrospectively assessed the association between serum placenta growth factor levels and clinicopathological findings and survival. Results: Expression of placenta growth factor was significantly higher in colorectal cancer tissues compared with non-tumor tissues. The mean serum placenta growth factor level in patients was significantly higher than that in controls and significantly higher in patients with large tumor, lymph-node involvement and distant metastasis. Conclusions: Elevated serum placenta growth factor levels are significantly associated with colorectal cancer development, lymph or distant invasive phenotypes and survival, especially in stage II or III patients.
Preoperative Vascular Endothelial Growth Factor Levels as a Prognostic Marker for Stage Ii or Iii Colorectal Cancer Patients
(Libertas Acad, 2011) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Purisa, Sevim; Dulger, A. Cumhur; Begenik, Huseyin; Tuzun, Sefa
Background: The aim of the present study was to determine whether serum vascular endothelial growth factor (VEGF) can provide prognostic information independent of carcinoembryonic antigen levels in patients undergoing curative surgery. Methods: Serum samples were collected from 158 patients with colorectal cancer and from 100 controls. Serum and tissue levels of VEGF were measured by enzyme-linked immunosorbent assay. Serum VEGF levels in colorectal cancer patients were compared with those in healthy controls, and we retrospectively assessed the association between serum VEGF levels and clinicopathologic findings and survival. Results: VEGF expression was significantly higher in colorectal cancer tissue compared with nontumor tissue. Mean serum VEGF levels in patients were significantly higher than those in controls, and significantly higher in patients with large tumors, lymph node involvement, and distant metastases. Conclusion: Elevated serum VEGF was significantly associated with poor survival, but was only an independent risk factor for poor survival in Stage II and/or III disease. Elevated serum VEGF is significantly associated with development of colorectal cancer, and lymph or distant invasive phenotypes and survival, especially in Stage II and III patients.
The Relationship Among Acute-Phase Response Proteins, Cytokines and Hormones in Cachectic Patients With Colon Cancer
(Bmc, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Hasirci, Ismail; Purisa, Sevim; Dulger, Ahmet Cumhur; Tuzun, Sefa
Backgraund: Acute-phase response proteins (APRP), cytokines and hormones have been claimed to be an independent prognostic factor of malignancies, however the basis for their association with prognosis remains unexplained. We suggest that in colon malignancies, as similar to pancreatic and lung cancers, changes in APRP are associated with angiogenesis. Methods: C-reactive protein (CRP), albumin, IL-1 alpha, IL-1 beta, IL-6, IL-8, IL-10, TNF-alpha, midkine, VEGF-A, VEGF-C, leptin, adiponectin, and ghrelin serum levels are studied in 126 colon cancer patients and 36 healthy subjects. Results: We found statistically significant difference and correlations between two groups. We found significantly higher serum CRP, IL-1 alpha, IL-1 beta,IL-6, IL-8, IL-10, TNF-alpha, VEGF-A, VEGF-C and leptin concentrations in patients relative to controls (p < 0.001). We found lower levels of the serum albumin, midkine, adiponectin and ghrelin in patients compared to control subjects (p < 0.001). Conclusions: Cachexia in patients with colon cancers is associated with changes in APRP, cytokines and hormone concentrations. These biomarkers and cachexia together have a direct relationship with accelerated angiogenesis. This may lead to a connection between the outcomes in malignancies and the biomarkers.
Relationship Between the Levels of Oxidative Stress in Mesenteric and Peripheral Serum and Clinicopathological Variables in Colorectal Cancer
(Galenos Publ House, 2012) Yucel, Ahmet Fikret; Kemik, Ozgur; Kemik, Ahu Sarbay; Purisa, Sevim; Tuzun, Ishak Sefa
Objective: To explore the differences existing between the levels of oxidative stress in peripheral and mesenteric serum in patients with colorectal cancer. Material and Methods: One hundred fifty patients with colorectal cancer who underwent surgery between May 2005 and March 2010 were prospectively analyzed. The differences between oxidative stress parameters in their peripheral and mesenteric blood were measured. The associations between peripheral and mesenteric levels and the staging and clinicopathological variables were investigated. Results: Oxidative stress parameters were higher in patients with advanced tumor staging (p<0.01), lymph node invasion (p<0.01), and venous invasion (p<0.01). Differences between oxidative stress parameters in peripheral and mesenteric blood samples were also observed. Conclusions: The mesenteric levels of the oxidative stress markers were higher than the peripheral levels in these colorectal cancer patients. Higher levels of these oxidative stress markers are associated with an advanced state of cancer.
Serum Levels of Vegf and Egf in Thyroid Gland Tumors
(2010) Purisa, Sevim; Kemik, Ahu Sarbay; Dülger, A.cumhur; Hasırcı, İsmail; Kemik, Özgür; Tüzün, Sefa; Adas, Mine
Amaç: VEGF ve EGF serum düzeyleri troid bezitümörlerinin patogenezinde ve onlarınbüyümesindde rol oynar. Hipotezimiz tiroid tumorhücreleri tarafından üretilen bu sitokininlerperiferal kanda düzeylerini yansıttığınısavunmaktayız.Metod: ELISA kit kullanarak tiroid bezi kanserli20 hastanın ve sağlıklı bireylerimizin periferalkanında bu sitokininleri ölçtük.Bulgular: Biz 10 tiroid adenomalı ve 10 tiroidpapillar karsinomlu hastaların serum VEGF veEGF düzeylerini sağlıklı bireylerimize göre,istatiksel olarak yüksek bulduk. Ancak, tiroidadenomlu ve tiroid papillar karsinomlu hastalararasında ise bu sitokininlerin konsantrasyonlarıarasında bir fark göremedik. Sonuç: Tirodi bezi tumor hücreleri tarafından busitokininlerin üretimindeki değişikliğin peripheralkan düzeylerini yansıttığını düşünmekteyiz. Aynızamanda VEGF ve EGF serum düzeyeleriarasındaki farklılık, tiroid tumor hücreleritarafından üretimini yüksek olması şeklindeaçıklanabilir ve foliküle ve endoteliyal hücreproliferasyonunu güçlü etkisini vurgulamaktadır.
Serum Procarboxypeptidase a and Carboxypeptidase a Levels in Pancreatic Disease
(Sage Publications Ltd, 2012) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Begenik, Huseyin; Dulger, Ahmet Cumhur; Purisa, Sevim; Tuzun, Sefa
Background and objectives: To determine the serum levels of procarboxypeptidase A (pro-CPA) and carboxypeptidase A (CPA) in patients with acute and chronic pancreatitis and pancreatic cancer. Materials and methods: Serum samples obtained from 96 patients with acute pancreatitis, 101 patients with chronic pancreatitis, 98 patients with pancreatic cancer and 96 control groups were assayed for biochemical parameters and serum pro-CPA and CPA. Results: Serum pro-CPA and CPA levels were significantly higher in acute and in chronic pancreatic cancer patients compared to control group (p < 0.001). Pancreatic cancer patients had significantly higher serum pro-CPA and CPA levels when compared with acute and chronic pancreatitis cases (p < 0.001). Conclusion: These data prove for increased pro-CPA and CPA levels as a biomarker for the diagnosis of pancreatitis and pancreatic cancer.
Serum Vascular Endothelial Growth Factor Receptor-3 Levels in Patients With Esophageal Squamous Cell Cancer
(Edizioni Luigi Pozzi, 2017) Sumer, Aziz; Demir, Ali; Kemik, Ahu Sarbay; Yavuz, Alpaslan; Suvak, Burak; Dulger, Ahmet Cumhur; Kemik, Ozgur
AIM: Esophageal cancer is one of the most aggressive tumors of the gastrointestinal tract. In this study, we quantified the serum vascular endothelial growth factor-3 (VEGFR-3) expression in patients with esophageal squamous cell carcinoma (ESCC) to evaluate the role of VEGFR-3 in ESCC. MATERIALS AND METHODS: Ninety five patients with ESCC were studied. Pre-therapy and preoperative samples were stored and ELISA was used to designate the concentrations of VEGFR-3. RESULTS: The serum values of VEGFR-3 were significantly higher in patients with ESCC than in healthy donors (p<0.0001). CONCLUSIONS: The results imply a very good sensitivity of VEGFR-3 in ESCC. VEGFR-3 may be a good diagnostic biomarker for ESCC.