Browsing by Author "Kemik, Ozgur"

Now showing 1 - 20 of 45

Are Serum Mac 2-Binding Protein Levels Elevated in Esophageal Cancer? a Control Study of Esophageal Squamous Cell Carcinoma Patients
(Hindawi Ltd, 2018) Cobanoglu, Ufuk; Mergan, Duygu; Dulger, Ahmet Cumhur; Celik, Sebahattin; Kemik, Ozgur; Sayir, Fuat
Objective. Elevated serum Mac 2-binding protein (M2BP) levels have been observed in some cancers. As far as we know, its importance has not been investigated in esophageal squamous cell carcinoma (ESCC). The investigated problem of this study was to evaluate whether there was a difference between ESCC patients and the control group in terms of M2BP. Also, we evaluated the diagnostic performance of serum M2BP alone or in combination with the CEA for patients with ESCC. Material and Methods. Blood serum samples were collected from 50 healthy donors and 150 patients with ESCC. M2BP levels of all 200 samples were quantified by ELISA (enzyme-linked immunosorbent assay). Patients who had been diagnosed with ESCC and did not have any other malignancies were enrolled to study. Results. The two groups did not significantly differ in terms of age (p > 0 05). In the control group, the mean serum M2BP level was 14.97 coproduct 3.46 ng/mL. The mean serum M2BP level of the ESCC patients was 176.65 +/- 22.14 ng/mL. The serum M2BP level was significantly higher in patients with ESCC than in the control group (p < 0 001). Gender was also comparable in both groups (p = 0 695). Conclusions. Our analysis demonstrated that this marker may be associated with the mechanism of the disease. Despite that serum M2BP is not a specific marker for ESCC, it can be used as an adjuvant biomarker for the diagnosis of ESCC.
B7-H3: a Useful Emerging Diagnostic Marker for Colon Cancer
(Hindawi Ltd, 2022) Bostanci, Ozgur; Sayin, Pinar; Kiziltan, Remzi; Algul, Sermin; Aydin, Mehmet Akif; Kemik, Ozgur
Background. Colon cancer is the second most common malignancy causing the majority of cancer-related deaths. B7-H3 concentrations have drawn major interest as possible diagnostic biomarkers of cancer. The aim of this study was to measure the preoperative serum B7-H3 levels and to determine those that are replaced in colon cancer. Method. We measured preoperative serum B7-H3 concentrations of 90 patients aged 57-69 years diagnosed with colon cancer and 50 age-matched healthy individuals. B7-H3 levels were determined using the sandwich enzyme-linked immunosorbent assay (ELISA). Patients were categorized by stage based on the TNM staging system, and the serum levels of B7-H3 were compared between patients with different TNM stages. Result. No statistically significant difference was found between the patient and control groups in terms of age and gender. Preoperative serum B7-H3 levels were statistically significantly higher in patients with colon cancer than in the healthy group (p < 0.001). Preoperative serum B7-H3 concentration of the stage IV patients was significantly higher than those of the patients with stage I and stage II disease. In addition, higher serum B7-H3 levels were associated with lymph node status and distant metastasis in colon cancer. Conclusion. We showed that B7-H3 is highly expressed in colon cancer and can be used as a candidate diagnostic biomarker and a potential target in colon cancer in future.
Complications Following Endoscopic Retrograde Cholangiopancreatography: Minimal Invasive Surgical Recommendations
(Public Library Science, 2014) Koc, Bora; Bircan, Huseyin Yuce; Adas, Gokhan; Kemik, Ozgur; Akcakaya, Adem; Yavuz, Alpaslan; Karahan, Servet
Background: ERCP has a complication rate ranging between 4% and 16% such as post-ERCP pancreatitis, hemorrhage, cholangitis and perforation. Perforation rate was reported as 0.08% to 1% and mortality rate up to 1.5%. Besides, injury related death rate is 16% to 18%. In this study we aimed to present a retrospective review of our experience with post ERCP-related perforations, reveal the type of injuries and management recommendations with the minimally invasive approaches. Methods: Medical records of 28 patients treated for ERCP-related perforations in Okmeydani Training and Research Hospital between March 2007 and March 2013 were reviewed retrospectively. Patient age, gender, comorbidities, ERCP indication, ERCP findings and details were analyzed. All previous and current clinical history, laboratory and radiological findings were used to assess the evaluation of perforations. Results: Between March 2007 and March 2013, 2972 ERCPs were performed, 28 (0.94%) of which resulted in ERCP-related perforations. 10 of them were men (35.8%) and 18 women (64.2%). Mean age was 53.36 +/- 14.12 years with a range of 28 to 78 years. 14 (50%) patients were managed conservatively, while 14 (50%) were managed surgically. In 6 patients, laparoscopic exploration was performed due to the failure of non-surgical management. In 6 of the patients that ERC-Prelated perforation was suspected during or within 2 hours after ERCP, underwent to surgery primarily. There were two mortalities. The mean length of hospitalization stay was 10.46 +/- 2.83 days. The overall mortality rate was 7.1%. Conclusion: Successful management of ERCP-related perforation requires immediate diagnosis and early decision to decide whether to manage conservatively or surgically. Although traditionally conventional surgical approaches have been suggested for the treatment of perforations, laparoscopic techniques may be used in well-chosen cases especially in type II, III and IV perforations.
Cutaneous Necrosis as a Result of Isosulphane Blue Injection in Mammarian Sentinel Lymph Node Mapping: Report of Two Cases
(Sage Publications Ltd, 2014) Bircan, Huseyin Y.; Ozcelik, Umit; Koc, Bora; Kemik, Ozgur; Demirag, Alp
Skin-sparing mastectomy with sentinel lymph node biopsy (SLNB) and synchronous breast reconstruction are widely used in breast cancer surgery nowadays. Difficulties in feeling confident in this technique and postoperative surgical complications are the major obstacles against the widespread usage of this technique. Compared with the other surgical techniques, the complications are hard to treat. Cutaneous necrosis because of methylene blue used for sentinel lymph node mapping in patients who underwent skin-sparing mastectomy and SLNB is already reported in the literature. We present here two cases with cutaneous necrosis because of isosulphane blue injection after skin-sparing mastectomy and SLNB as a rare complication of dye injection.
The Effects of Aging on Lymphocyte Subgroups in Males and Females
(int Scientific Literature, inc, 2014) Inal, Ali; Koc, Bora; Bircan, Huseyin Yuce; Ogan, Ebru; Yavuz, Alpaslan; Kemik, Ozgur
Background: Age-associated immune senescence is a catch-all phrase that has been used to describe a plethora of changes to the immune system across the lifespan. Aging is associated with a decline in immune function. Our aim in this study was to investigate how lymphocyte subgroups in peripheral blood are affected by aging among males and females. Material/Methods: Study participants were 70 healthy individuals from 3 different age groups, observed from January 2010 to January 2012. The average levels of CD3+, CD4+, CD8+, CD19+, CD16+/CD56+, CD3+/CD69+, and CD19+/CD69+ were determined for each group and compared in terms of age and sex. Results: We found significant reduction in the level of CD3+T cells related with age, but no significant changes in CD19+ B cell levels (p<0.005). Aging significantly reduces activated B cell (CD19+/CD69+) levels in males (p<0.005). Conclusions: Our results show that there may be differences between males and females in terms of immune senescence.
Epcam Is a New Potential Serum Biomarker for Early Gastric Cancer
(Bayrakol Medical Publisher, 2023) Eroglu, Ersan; Kiziltan, Remzi; Algul, Sermin; Kemik, Ozgur; Altinli, Ediz
Aim: Overexpression of epithelial cell adhesion molecule (EpCAM) has been shown in a wide spectrum of epithelial cancers. In this study we aimed to examine the utility of serum EpCAM levels as a new marker for early diagnosis of gastric cancer, and to determine its association with different stages of gastric cancer. Material and Methods: A total of 88 patients who underwent resection due to gastric cancer and 44 healthy subjects as the control group were included in the study. The diagnosis of gastric cancer was confirmed by a combination of upper gastrointestinal endoscopy, endoscopic ultrasonography, and histopathology. EpCAM levels were evaluated using the enzyme-linked immunosorbent assay (ELISA) method following the instructions of the manufacturer. Results: The mean preoperative EpCAM level was found as 235.7 +/- 34.2 pg/mL in the patient group and 42.5 +/- 2.38 pg/mL in the control group. The mean preoperative serum EpCAM level was found to be significantly higher in gastric cancer patients compared to the control group (p<0.001). In addition, mean EpCAM levels were higher in patients with class T4 and T2 compared to T1. Mean EpCAM levels were also significantly higher in N1 and N2 classes compared to N0, and in M1 compared to M0 (all, p<0.001). Discussion: The results of this study indicate that the elevated expression of the EpCAM levels can predispose to the development of gastric cancer. Thus, the analysis of this marker could be a useful biomarker for screening patients with gastric cancer.
Evaluation of Preoperative and Postoperative Total Serum Sialic Acid Levels in Patients With Colon Cancer
(Edizioni Luigi Pozzi, 2020) Iliklerden, Umit Haluk; Peksen, Caghan; Kalayci, Tolga; Kemik, Ozgur
AIM: The aim of this study was to compare the preoperative and postoperative (48th hour) total serum sialic acid levels of the patients with colon cancer and to investigate if the total serum sialic acid levels can be used as a tumor marker in colon cancer. METHODS: Preoperative and postoperative (48th hour) total serum sialic acid levels of 100 patients that were diagnosed with colon cancer and 70 healthy individuals were examined. All total serum sialic acid levels were determined by the methods of Warren. RESULTS: Total sialic acid levels of both patient groups were significantly higher when compared to the control group (p<0.0001). Also, highly significant difference was found between preoperative and postoperative total serum sialic acid levels (p<0.001). CONCLUSION: Evaluation of total serum sialic acid levels may play a critical role in colon cancers. Total serum sialic acid levels may serve as a non-invasive tool for early diagnosis of colon cancer.
Evaluation of the Open and Laparoscopic Appendectomy Operations With Respect To Their Effect on Serum Il-6 Levels
(Turkish Assoc Trauma Emergency Surgery, 2016) Bartin, Mehmet Kadir; Kemik, Ozgur; Caparlar, Mehmet Ali; Bostanci, Mustafa Taner; Oner, Muzaffer Onder
BACKGROUND: Postoperative serum inflammatory cytokine levels are thought to reflect the magnitude of surgical stress. Cytokine interleukin-6 (IL-6) is an early marker of systemic inflammatory response and tissue damage. This study evaluated levels of IL-6 after open and laparoscopic appendectomy to compare the degree of surgical stress associated with these procedures. METHODS: IL-6 levels were measured pre- and postoperatively in the plasma of 200 consecutive patients with a diagnosis of acute appendicitis. After preoperative randomization, 100 patients underwent open appendectomy, and 100 patients underwent laparoscopic appendectomy. RESULTS: Preoperative concentrations of IL-6 were 65.22 +/- 4.76 pg/mL in the open appendectomy group and 65.73 +/- 6.34 pg/mL in the laparoscopic appendectomy group (p=0.752). Postoperative levels were 105.28 +/- 16.14 pg/mL and 76.11 +/- 16.18 pg/mL, respectively (p<0.05). CONCLUSION: Lower postoperative serum IL-6 levels suggest that laparoscopic surgery is associated with lower degree of surgical stress. Laparoscopic appendectomy has significant advantage over open appendectomy due to more rapid postoperative recovery.
Gossypiboma: Retained Surgical Sponge After a Gynecologic Procedure
(Hindawi Ltd, 2010) Sumer, Aziz; Carparlar, M. Ali; Uslukaya, Omer; Bayrak, Vedat; Kotan, Cetin; Kemik, Ozgur; Iliklerden, Umit
We report on a case of gossypiboma. A 54-year-old woman was admitted to our hospital with abdominal mass. She had undergone a caesarean operation 23 years previously. The mass in the right abdominal quadrant was suspected by abdominal computed tomography and magnetic resonance imaging. The mass was removed by laparotomy excision and the final diagnosis was gossypiboma.
Hepatic Encephalopathy in Connection With Budd-Chiari Syndrome Due To Infection With Echinococcus Multilocularis: a Case Report
(Elmer Press inc, 2011) Dulger, Ahmet Cumhur; Kemik, Ozgur; Selvi, Fatih; Begenik, Huseyin; Emre, Habib; Erdur, Fatih Mehmet
Budd-Chiari syndrome (BCS) is a hepatic venous outflow block generally resulting from disorders affecting hepatic venous system. Elevated hepatic venous pressure results in portal hypertension. BCS may also cause hepatic encephalopathy. Echinococcus multilocularis is a tapeworm parasite and the natural course of the disease may affect liver parenchyma as well as hepatic venous tree. It is the most terrible parasitic disease of the liver and is easily confused with hepatic malignancies. Albendazole therapy may suppress disease progression. Alveolar echinococcosis of the liver rarely causes Budd-Chiari syndrome-related hepatic encephalopathy (HE). We report a rare case of alveolar echinococcosis-related BCS with HE, who was successfully managed by rifaximin and albendazole.
Human Vascular Adhesion Protein-1 (vap-1): Serum Levels for Hepatocellular Carcinoma in Non-Alcoholic and Alcoholic Fatty Liver Disease
(Biomed Central Ltd, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Itik, Veyis; Dulger, Ahmet Cumhur; Purisa, Sevim; Tuzun, Sefa
Background: The incidence of hepatocellular cancer in complicated alcoholic and non-alcoholic fatty liver diseases is on the rise in western countries as well in our country. Vascular adhesion protein-1 (VAP-1) levels have been presented as new marker. In our study protocol, we assessed the value of this serum protein, as a newly postulant biomarker for hepatocellular cancer in patients with a history of alcoholic and non-alcoholic fatty liver diseases. Methods: Pre-operative serum samples from 55 patients with hepatocellular cancer with a history of alcoholic and non-alcoholic fatty liver diseases and patients with cirrhosis were assessed by a quantitative sandwich ELISA using anti-VAP-1 mAbs. This technique is used to determine the levels of soluble VAP-1 (sVAP-1) in the serum. Results: sVAP-1 levels were evaluated in patients with hepatocellular cancer and liver cirrhosis. There was a significant difference in mean VAP-1 levels between groups. Serum VAP-1 levels were found higher in patients with hepatocellular cancer. Conclusion: These findings indicate that the serum level of sVAP-1 might be a beneficial marker of disease activity in chronic liver diseases.
Is Combined Therapy More Effective Than Growth Hormone or Hyperbaric Oxygen Alone in the Healing of Left Ischemic and Non-Ischemic Colonic Anastomoses
(Hospital Clinicas, Univ Sao Paulo, 2013) Adas, Mine; Kemik, Ozgur; Adas, Gokhan; Arikan, Soykan; Kuntsal, Leyla; Kapran, Yersu; Toklu, Akin Savas
OBJECTIVE: Our aim was to investigate the effects of growth hormone (GH), hyperbaric oxygen and combined therapy on normal and ischemic colonic anastomoses in rats. METHODS: Eighty male Wistar rats were divided into eight groups (n = 10). In the first four groups, non-ischemic colonic anastomosis was performed, whereas in the remaining four groups, ischemic colonic anastomosis was performed. In groups 5, 6, 7, and 8, colonic ischemia was established by ligating 2 cm of the mesocolon on either side of the anastomosis. The control groups (1 and 5) received no treatment. Hyperbaric oxygen therapy was initiated immediately after surgery and continued for 4 days in groups 3 and 4. Groups 2 and 6 received recombinant human growth hormone, whereas groups 4 and 8 received GH and hyperbaric oxygen treatment. Relaparotomy was performed on postoperative day 4, and a perianastomotic colon segment 2 cm in length was excised for the detection of biochemical and mechanical parameters of anastomotic healing and histopathological evaluation. RESULTS: Combined treatment with hyperbaric oxygen and GH increased the mean bursting pressure values in all of the groups, and a statistically significant increase was noted in the ischemic groups compared to the controls (p < 0.05). This improvement was more evident in the ischemic and normal groups treated with combined therapy. In addition, a histopathological evaluation of anastomotic neovascularization and collagen deposition showed significant differences among the groups. CONCLUSIONS: Combined treatment with recombinant human growth hormone and hyperbaric oxygen resulted in a favorable therapeutic effect on the healing of ischemic colonic anastomoses.
Laminin: a New Biomarker for Gastric Cancer
(Kuwait Medical Assoc, 2024) Kalayci, Tolga; Yilmaz, Ozkan; Iliklerden, Umit Haluk; Kemik, Ozgur; Kotan, Mehmet Cetin
Objective: To evaluate the diagnostic and prognostic utility of serum laminin levels in gastric cancer Design: A prospective and observational study Setting: Department of General Surgery, Van Yuzuncu Yil University Medical Faculty, Van, Turkey Subjects: Between June 2018 and September 2018, eighty patients who were diagnosed with gastric cancer as the patient group, and forty volunteers as the control group were included in the study. Intervention: From each patient, 3 ml of peripheral blood sample was collected. Main outcome measures: Enzyme-linked immunosorbent assay kit for human laminin were used for the detection of laminin in samples of serum. Data on patient demographics (age, gender) and serum laminin levels were recorded in both control and gastric cancer groups. In gastric cancer patients, serum laminin levels were further analyzed with respect to tumor stages and tumor size. Results: Serum laminin levels were significantly higher in gastric cancer patients [median (min-max): 205 (165-483) vs. 12 (9-18) ng/mL, P <0.001]. Laminin levels were higher in patients with advanced invasion depth, distant organ metastasis and lymph node metastasis ( P <0.001). The sensitivity and specificity determined from the receiver operating characteristic curves at cut-off level of 70 were 95% and 97% for serum laminin, respectively. Conclusion: The serum concentration of laminin can be used as a biomarker at the time of diagnosis for gastric cancer with high sensitivity and specificity. In addition, laminin can be used to discriminate between earlier, advanced or metastatic stages of gastric cancer.
Laparoscopic Treatment of Gallstone Ileus
(Sage Publications Ltd, 2014) Bircan, Huseyin Y.; Koc, Bora; Ozcelik, Umit; Kemik, Ozgur; Demirag, Alp
Gallstone ileus is a rare complication of cholelithiasis that has high morbidity and mortality. An intestinal obstruction can be caused by migration of a large gallstone through a biliary enteric fistula or by impaction within the intestinal tract. In this study, we present the case of an 81-year-old woman with a mechanical bowel obstruction by a gallstone that was treated by laparoscopy.
Levels of Matrix Metalloproteinase-1 and Tissue Inhibitors of Metalloproteinase-1 in Gastric Cancer
(Baishideng Publ Grp Co Ltd, 2011) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Dulger, Ahmet Cumhur; Adas, Mine; Begenik, Huseyin; Kotan, Cetin
AIM: To evaluate the levels of preoperative serum matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in gastric cancer. METHODS: One hundred gastric cancer patients who underwent gastrectomy were enrolled in this study. The serum concentrations of MMP-1 and TIMP-1 in these patients and in fifty healthy controls were determined using an enzyme-linked immunosorbent assay. RESULTS: Higher serum MMP-1 and TIMP-1 levels were observed in patients than in controls (P < 0.001). Serum MMP-1 and TIMP-1 levels were positively associated with morphological appearance, tumor size, depth of wall invasion, lymph node metastasis, liver metastasis, perineural invasion, and pathological stage. They were not significantly associated with age, gender, tumor location, or histological type. CONCLUSION: Increased MMP-1 and TIMP-1 were associated with gastric cancer. Although these markers are not good markers for diagnosis, these markers show in advanced gastric cancer. (C) 2011 Baishideng. All rights reserved.
The Levels of the D-Dimer and All Haemostatic Factors in Patients With and Without Deep Venous Thrombosis
(Yerkure Tanitim & Yayincilik Hizmetleri A S, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Purisa, Sevim; Tuzun, Sefa; Kotan, Cetin
Objective: Although improved diagnostic tools and treatment approach, deep venous thrombosis is still an important cause of morbidity and mortality. Deep venous thrombosis is a common condition that affects more than 250 000 patients each year in the United States. In spite of limitations of the available diagnostic tools for detecting deep venous thrombosis, laboratory tests such as plasma D-dimer has been gained much interest recently. So, we aimed to investigate the D-dimer and all hemostatic factors in patients with deep vein thrombosis. Material and Methods: We investigated the levels of the D-dimer and Von Willebrand factor by enzyme linked immunosorbent assay (ELISA) in 120 patients with deep venous thrombosis and in 115 patients without deep venous thrombosis. We used standard methods for detecting the levels of the cholesterol, creatinin, INR, factor VIII: C ratio, and aPTT. We used Clauss method for detecting the fibrinogen levels. Results: We obtained important results in patients with deep venous thrombosis compared to in patients without deep vein thrombosis. Age, BMI, aPTT, INR, and total cholesterol levels were not found statistically differences in patients with deep venous thrombosis (p> 0,05). Plasma D-dimer, creatinin, factor VII: C, and Von Willebrand factor levels were found higher in patients with deep venous thrombosis than in patients without deep venous thrombosis (respectively, p<0,001, p<0,01). But, plasma fibrinogen levels were not found lower in patients with deep venous thrombosis (p<0,01). Conclusion: In conclusion, we suggested that the plasma D-dimer and hemostatic factor levels may play an important role in diagnosis and treatment of deep venous thrombosis.
Lipid Peroxidation and Transforming Growth Factor-Β1 Levels in Gastric Cancer at Pathologic Stages
(Galenos Publ House, 2012) Tuzun, Sefa; Yucel, Ahmet Fikret; Pergel, Ahmet; Kemik, Ahu Sarbay; Kemik, Ozgur
Objective: High levels of TGF-beta 1 and enhanced TGF-beta 1 receptor signaling are related to the pathology of gastric cancer. This effect is caused by oxidative stress and lipid peroxidation products. The aim of this study was to investigate the levels of TGF-beta 1 and lipid peroxidation products in gastric cancer patients and their correlation with pathologic stage. Material and Methods: Lipid peroxidation products and TGF-beta 1 levels were studied in the serum samples of 50 gastric cancer patients and 18 control subjects. Results: HNE-protein adducts and TGF-beta 1 levels were significantly higher in T2, T3 and T4 gastric cancers than in either the T1 stage or controls (p<0.001). Pathologic stage was correlated with TGF-beta 1 levels (r=0.702, p<0.05). Conclusion: These markers production may contribute to tumor angiogenesis and aid in the prognosis of the gastric cancer.
Massive Alimentary Tract Bleeding Due To Cytomegalovirus Infection in an Elderly Patient
(Pagepress Publ, 2014) Koc, Bora; Bircan, Huseyin Yuce; Altaner, Semsi; Cinar, Ozlem; Ozcelik, Umit; Yavuz, Alpaslan; Kemik, Ozgur
In recent years, cytomegalovirus (CMV) has been recognized as an important common pathogen in immunocompromized patients. This is due to the increasing number of immunosuppressive medications, intensive cancer chemotherapy use, recurrent transplantations, progressively aging population, and the higher number of human immunodeficiency virus infections. Cytomegalovirus infection especially interests the gastrointestinal tract, anywhere. from the mouth to the anus. Namely, the most commonly affected area is the colon, followed by duodenum, stomach, esophagus and small intestine. The most frequent manifestations of CMV colitis are: diarrhea. fever, gastrointestinal bleeding and abdominal pain. We report here the case of an 82-year-old woman, who was treated for non Hodgkin lymphoma: she was admitted to the emergency, department for abdominal pain and diffuse arthralgia following massive upper and lower-gastrointestinal bleeding, due to duodenal and colonic ulcers related to CMV infection.
Matrix Metalloproteinases in Pathogenesis of Hemorrhoidal Disease
(Southeastern Surgical Congress, 2013) Kisli, Erol; Kemik, Ahu; Sumer, Aziz; Kemik, Ozgur
The aim of this study is to investigate the accuracy of serum matrix metalloproteinase (MMP) levels in an effort to find a reliable factor that may play an important role in pathogenesis of hemorrhoidal disease. Twenty control subjects and 21 Grade I, 19 Grade II, 20 Grade III, and 21 Grade IV patients with internal hemorrhoid were included in this prospective study. The mean ages of control subjects were 47.65 +/- 6.71 standard deviation (SD) years (range, 37 to 60 years). The mean age of internal Grade I, Grade II, Grade III, and Grade IV patients with internal hemorrhoid were 48.85 +/- 6.44, 47.20 +/- 6.75, 44.90 +/- 6.13, and 42.95 +/- 3.49 SD years (ranges, 38 to 58, 38 to 60, 34 to 55, and 38 to 50 years), respectively. Ten milliliters of blood was taken from all subjects. Enzymelinked immunosorbent assay (ELISA) for MMP-1, -2, -7, and -9 levels were performed using an ELISA kit (R&D Systems) following the manufacturer's instructions. There was an important difference between Grade I and Grade II groups in the serum levels of MMP-9 (P <0.01). Patients with Grade III hemorrhoidal disease had significantly higher serum levels of all MMP than patients with Grade I and Grade II hemorrhoidal disease (P < 0.001). Also, patients with Grade 4 hemorrhoidal disease had higher serum levels of MMP-7 and -9 according to Grade I, II, and III groups (P < 0.01, 0.001). High serum levels of MMP are present in patients with hemorrhoids, suggesting the possible mechanism in the pathogenesis of hemorrhoids.
Nidogen-2: a New Biomarker in Colon Cancer Patients
(Edizioni Luigi Pozzi, 2022) Kiziltan, Remzi; Cikman, Oztekin; Algul, Sermin; Aydin, Mehmet Akif; Kemik, Ozgur
AIM: In the present study, we followed Nidogen-2 levels and clinicopathological parameters of patients with colon cancer. MATERIAL AND METHODS: Eighty-eight patients (F/M, 43/45; Mean age +/- SD, 57.86 +/- 1.78 years) were included. The results of serum Nidogen-2 levels were shown with respect to stage, gender, age, and metastasis. Nidogen-2 levels in the sera of colon cancer patients and healthy donors were analyzed with ELISA. RESULTS: The expression levels were significantly higher in patients (1010.8 +/- 184.36 pg/mL) than in healthy subjects (51.85 +/- 1.44 pg/mL; p<0.001). Moreover, the Nidogen-2 expression significantly increased in the clinical stages of colon cancer (p<0.01). The Nidogen-2 levels did not vary by patient age or gender. DISCUSSION: Under normal conditions, Nidogen-2 is a basal membrane protein. Nidogen-2 is primarily expressed in the extracellular matrix. Nidogen-2 has been defined as a major means to analyze the molecular pathways involved in cancer development and progression. Besides its important functions, it has been hypothesized that secreted Nidogen-2 may be a diagnostic biomarker for cancer detection. CONLUSION: These findings suggest that increased expression of Nidogen-2 may have great pathological importance in the development of colon cancer and may also show a diagnostic value for colon cancer.