Browsing by Author "Khalilzadeh, Balal"

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Advances in Nanomaterial Based Optical Biosensing and Bioimaging of Apoptosis Via Caspase-3 Activity: a Review
(Springer Wien, 2018) Khalilzadeh, Balal; Shadjou, Nasrin; Kanberoglu, Gulsah Saydan; Afsharan, Hadi; de la Guardia, Miguel; Charoudeh, Hojjatollah Nozad; Rashidi, Mohammad-Reza
Caspase-3 plays a vital role in intrinsic and extrinsic pathways of programed cell death and in cell proliferation. Its detection is an important tool for early detection of some cancers and apoptosis-related diseases, and for monitoring the efficacy of pharmaceuticals and of chemo- and radiotherapy of cancers. This review (with 72 references) summarizes nanomaterial based methods for signal amplification in optical methods for the determination of caspase-3 activity. Following an introduction into the field, a first large section covers optical assays, with subsections on luminescent and chemiluminescence, fluorometric (including FRET based), and colorimetric assays. Further section summarize methods for bioimaging of caspase-3. A concluding section covers current challenges and future perspectives.
Development of a Reliable Microrna Based Electrochemical Genosensor for Monitoring of Mir-146a, as Key Regulatory Agent of Neurodegenerative Disease
(Elsevier Science Bv, 2019) Khalilzadeh, Balal; Rashidi, Mohammad; Soleimanian, Alireza; Tajalli, Habib; Kanberoglu, Gulsah Saydan; Baradaran, Behzad; Rashidi, Mohammad-Reza
A MicroRNA (miR) based electrochemical method for quantification of miR-146a, a known biomarker for neurodegenerative disease, was developed. In this bioassay, the capture microRNA (C-miR) was self-assembled on the gold surface and used for quantification of target microRNA (T-miR) of miR-146a. For this purpose, an optimized concentration of C-miR was immobilized on the surface of gold electrode and used for capture of target analyte (T-miR). All of preparation steps were characterized by electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and atomic force microscopy (AFM). At the optimized conditions, the linear dynamic range, limit of quantification and relative standard deviation of the proposed bioassay were obtained as 10 pM to 1 mu M, 10 pM and 1.59%, respectively. The unprocessed human serum sample was used as a real sample and the results fully confirm that the designed microRNA based biosensor is capable for detection of miR-146a as neurodegenerative disease biomarker. The developed method offers a more precise and high sensitive tool to be used in clinical applications for early detection of neurodegenerative disease like Alzheimer's and Parkinson. (C) 2019 Elsevier B.V. All rights reserved.
Electrochemical Biosensors as a Novel Platform in the Identification of Listeriosis Infection
(Mdpi, 2023) Mehrannia, Leila; Khalilzadeh, Balal; Rahbarghazi, Reza; Milani, Morteza; Kanberoglu, Gulsah Saydan; Yousefi, Hadi; Erk, Nevin
Listeria monocytogenes (L.M.) is a gram-positive bacillus with wide distribution in the environment. This bacterium contaminates water sources and food products and can be transmitted to the human population. The infection caused by L.M. is called listeriosis and is common in pregnant women, immune-deficient patients, and older adults. Based on the released statistics, listeriosis has a high rate of hospitalization and mortality; thus, rapid and timely detection of food contamination and listeriosis cases is necessary. During the last few decades, biosensors have been used for the detection and monitoring of varied bacteria species. These devices are detection platforms with great sensitivity and low detection limits. Among different types of biosensors, electrochemical biosensors have a high capability to circumvent several drawbacks associated with the application of conventional laboratory techniques. In this review article, different electrochemical biosensor types used for the detection of listeriosis were discussed in terms of actuators, bioreceptors, specific working electrodes, and signal amplification. We hope that this review will facilitate researchers to access a complete and comprehensive template for pathogen detection based on the different formats of electrochemical biosensors.
Electrochemiluminescence Methods Using Cds Quantum Dots in Aptamer-Based Thrombin Biosensors: a Comparative Study
(Springer Wien, 2020) Isildak, Ibrahim; Navaeipour, Farzaneh; Afsharan, Hadi; Kanberoglu, Gulsah Soydan; Agir, Ismail; Ozer, Tugba; Khalilzadeh, Balal
The detection of thrombin by using CdS nanocrystals (CdS NCs), gold nanoparticles (AuNPs) and luminol is investigated in this work. Thrombin is detected by three methods. One is called the quenching method. It is based on the quenching effect of AuNPs on the yellow fluorescence of CdS NCs (with excitation/emission wavelengths of 355/550 nm) when placed adjacent to CdS NCs. The second method (called amplification method) is based on an amplification mechanism in which the plasmonics on the AuNPs enhance the emission of CdS NCs through distance related Forster resonance energy transfer (FRET). The third method is ratiometric and based on the emission by two luminophores, viz. CdS NCs and luminol. In this method, by increasing the concentration of thrombin, the intensity of CdS NCs decreases, while that of luminol increases. The results showed that ratiometric method was most sensitive (with an LOD of 500 fg.mL(-1)), followed by the amplification method (6.5 pg.mL(-1)) and the quenching method (92 pg.mL(-1)). Hence, the latter is less useful.
Electrodeposition of Cerium Oxide Nanoparticles on the Graphenized Carbon Ceramic Electrode (Gcce) for the Sensitive Determination of Isoprenaline in Human Serum by Differential Pulse Voltammetry (Dpv)
(Taylor & Francis inc, 2022) Shafaei, Sepideh; Akbari Nakhjavani, Sattar; Kanberoglu, Gulsah Saydan; Khalilzadeh, Balal; Mohammad-Rezaei, Rahim
Cerium oxide nanoparticles (CeO2) are widely used as the electrocatalyst in solar cells, fuel cells, water splitting, and electroanalytical experiments. Therefore, controlled electrodeposition of CeO2 on the surface of electrodes may improve the performance of electrochemical devices. CeO2 was electrochemically deposited on the surface of a graphenized carbon ceramic electrode (GCCE) for the determination of isoprenaline in biological samples. The pH, temperature, deposition time, and applied potential were optimized for the electrodeposition. According to differential pulse voltammetry, the linear range, detection limit, and sensitivity of the designed assay were from 5 to 310 nM, 3.03 nM and 25.21 x 10(-5 )mu A mu M(-1 )cm(-2), respectively. Easy preparation, low cost, and high production repeatability of the developed CeO2/GCCE suggest further applications. The prepared modified electrode was successfully utilized for the determination of isoprenaline in human serum with satisfactory results.
Preparation of Cerium Oxide-Mwcnts Nanocomposite Bulk Modified Carbon Ceramic Electrode: a Sensitive Sensor for Tamoxifen Determination in Human Serum Samples
(Springer, 2021) Shafaei, Sepideh; Hosseinzadeh, Elyas; Kanberoglu, Gulsah Saydan; Khalilzadeh, Balal; Mohammad-Rezaei, Rahim
In this study, cerium oxide and multi-walled carbon nanotubes nanocomposite were incorporated into the carbon ceramic electrode (CeO2-MWCNTs/CCE) as a renewable electrode for the electrocatalytic purposes. To demonstrate capability of the fabricated electrode, determination of tamoxifen as an important anticancer drug with differential pulse voltammetry technique was evaluated in details. Linear range, limit of detection and sensitivity of the developed sensor were found to be 0.2-40 nM, 0.132 nM and 1.478 mu A nM(-1) cm(-2), respectively. Ease of production, low cost and high electron transfer rate of the CeO2-MWCNTs/CCE promises it as a novel electro-analytical tool for determination of important species in real samples.