Browsing by Author "Koçak, Y."

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Investigation of Antioxidant and Cytoprotective Effects of Allium Schoenoprasum L (Sirmo) Plant Ethanol Extract in Liver Damage Caused by Carbontetrachloride in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Koçak, Y.; Yücel, U.M.
Liver diseases, morbidity and mortality rates are increasing in the world and existing drugs are insufficient for treatment. For this reason, there has been an increased interest in the discovery of new drugs against liver damage and the search for alternative therapies. This study investigated the cytoprotective and antioxidant effects of Allium schoenoprasum (AS) extract against carbon tetrachloride (CCl4) toxicity in rats. The in vivo experimental procedure was established as follows; Group 1, control (C) animals; Group 2, carboxymethylcellulose ( CMC); Group 3, CCl4; Group 4, Legalon (LGL, silymarin); Groups 5, 6 and 7 were treated with AS (100, 200 and 400 mg kg-1) extract only. Groups 8,9 and 10 were administered AS+CCl4. On the 11th day of the experiment, the animals were sacrificed and analyzed . According to the histopathological and biochemical analysis results; It was found that AS extract reduced tissue damage in a dose-dependent manner against CCl4 toxicity. When antioxidant and oxidative biomarkers were examined, positive effects of LGL and AS were observed in correcting the changed oxidant/antioxidant balance status due to oxidative stress caused by CCl4. The results showed that AS had a dose-dependent hepatoprotective effect against CCl4-induced liver toxicity and it was evaluated that thi s effect might be due to its antioxidant activity. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of Total Flavonoid, Dpph Radical Scavenging, Lipid Peroxidation and Antimicrobial Activity of Allium Schoenoprasum L. Plant Growing in Van Region
(Centenary University, 2020) Koçak, Y.; Oto, G.; Meydan, İ.; Şeçkin, H.
This study, the effect of ethanol extract of Allium schoenoprasum plant was investigated on some antioxidant parameters and pathogenic microorganisms that affect human health. In this coverage, DPPH (1,1-diphenyl-2-picrilhydrazyl) method was used to determine the antioxidant effect of A. schoenoprasum ethanol extract. Lipid peroxidation prevention activity was evaluated by TBA (Thiobarbituric acid) method. Besides, the antimicrobial activity of plant extract on some pathogenic bacteria was investigated via disk diffusion method. Quercetin was used to determine total flavonoid components. According to our findings, the total flavonoid component amount found in 0.2 mg/ml ethanol extract was found equivalent to quercetin with 44.465 ± 0.204 µg/ml. In addition, A. schoenoprasum showed that it was effective compared to BHA (Beta hydroxy acid), which is the positive control of lipid peroxidation activity. While the lowest and highest concentration values of DPPH radical Scavenging activity were 60,125 - 96,285%, these values were found to be 82.178 - 97.357% for BHA. Ethanol extract of A. schoenoprasum, was determined to show antimicrobial activity against some pathogenic microorganisms such as Bacillus subtilis, Escherichiacoli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans. © 2020, Centenary University. All rights reserved.