Browsing by Author "Komuroglu, Ahmet Ufuk"

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Betatrophin Association With Serum Triglyceride Levels in Obstructive Sleep Apnea Patients
(Wolters Kluwer Medknow Publications, 2019) Sertogullarindan, Bunyamin; Komuroglu, Ahmet Ufuk; Ucler, Rifki; Gunbatar, Hulya; Sunnetcioglu, Aysel; Cokluk, Erdem
Background: Obstructive sleep apnea (OSA) is a common sleep problem, in which patients are at increased risk for metabolic and cardiovascular problems, including metabolic syndrome, diabetes mellitus (DM), and dyslipidemia. Betatrophin is a novel protein that regulates fatty acid and triglyceride (TG) metabolism and is related to obesity and metabolic abnormalities, including metabolic syndrome, DM, and dyslipidemia. Although OSA and betatrophin share common abnormalities, their relationship has not been investigated. AIM: The aim of this study is to investigate the relationships among betatrophin, OSA, and the serum lipid profile. METHODS: Ninety consecutive patients with suspected OSA underwent polysomnography (PSG) to confirm OSA. Plasma betatrophin, leptin, adiponectin, and the full lipid profile were analyzed. The patients were categorized as OSA or control based on the apnea-hypopnea index (AHI). RESULTS: About 61% of patients had OSA, and 39% had normal PSG. The levels of betatrophin, leptin, and adiponectin were higher in patients with OSA (256.59 +/- 29.35, 374.20 +/- 37.93, and 17.86 +/- 2.63 mu g/mL, respectively) compared to the controls (141.86 +/- 26.20, 205.53 +/- 14.75, and 7.52 +/- 1.02 mu g/mL, respectively). Betatrophin levels were correlated with the AHI, leptin (r = 0.413, P = 0.002, r = 0.782, P = 0.000). TG levels were significantly higher, and high-density lipoprotein cholesterol (HDL-C) levels were lower, in OSA patients compared to controls (244 +/- 20.33 vs. 138 +/- 14.89, and 37.21 +/- 1.26 vs. 43.78 +/- 1.62, respectively). The TG level was correlated with betatrophin (r = 0.353, P = 0.013). Multiple regression analysis showed that the AHI, leptin, and arousals were independent predictors of betatrophin level (B = 1.70 P = 0.046 95%, B = 0.56 P < 0.005, and B = 1, 2, P = 0.003, respectively). CONCLUSIONS: Our results suggest a complex relationship between OSA, betatrophin, TG, and HDL, as well as other adipokines. Our results require further investigation to assess this complex association and re-evaluate previous related studies.
Cardiovascular Evaluation and Serum Paraoxonase-1 Levels in Adolescents With Polycystic Ovary Syndrome
(Taylor & Francis inc, 2020) Cetin, Mecnun; Tuncdemir, Perihan; Karaman, Kamuran; Yel, Servet; Karaman, Erbil; Ozgokce, Mesut; Komuroglu, Ahmet Ufuk
The aim of our study was to evaluate whether cardiovascular disease risks seen in adults with polycystic ovary syndrome (PCOS) develop in adolescents with PCOS using conventional Doppler echocardiography (CDE) and tissue Doppler echocardiography (TDE) or not. The other aim was to investigate the association of paraoxonase-1 (PON-1) level with cardiovascular parameters. 30 PCOS patients and 30 control patients were included in the study. All patients were evaluated with TDE and CDE. Paraoxonase-1 levels of both groups were studied. In CDE study, myocardial performance index (MPI) was higher in the PCOS group than in the control group (0.54 +/- 0.11, 0.50 +/- 0.12, p = .049, respectively). In the TDE study, early diastolic myocardial velocity (E)'/late diastolic myocardial velocity (A ') was lower in PCOS group than in the control group (2.07 +/- 0.08, 2.44 +/- 0.10, p = .008, respectively). PON-1 was higher in PCOS group than in the control group (26.81 +/- 3.05, 18.68 +/- 1.18, p = .011, respectively). Cardiovascular disease risks, which are among the long-term complications of PCOS, seem to begin from the early stage of PCOS. The high PON-1 level was thought to increase in response to increased oxidative stress in PCOS.Impact statement What is already known on this subject? Polycystic ovary syndrome (PCOS) is one of the most commonly seen endocrinopathy in the adolescent age group. PCOS has detrimental effects on the cardiovascular system in the adult population which is reported in many studies. What the results of this study add? The result of this study showed that cardiovascular effects, which are among the long-term complications of PCOS, seem to begin from the early stage of PCOS. And also, serum paraoxonase-1 level increases in response to the oxidative stress in the adolescent with PCOS.
Chemopreventive Efficacy of Juniper Berry Oil (Juniperus Communis L.) on Azoxymethane-Induced Colon Carcinogenesis in Rat
(Routledge Journals, Taylor & Francis Ltd, 2021) Yaman, Turan; Uyar, Ahmet; Komuroglu, Ahmet Ufuk; Keles, Omer Faruk; Yener, Zabit
The aim of this study was to investigate the chemopreventive effects of juniper berry (JB) oil on azoxymethane (AOM)-induced colon cancer in rats. Thirty-two male Wistar albino rats were allocated into four groups: Control, AOM, AOM?+?JB, and JB groups. Whereas the control group was fed with standard pellet feed, the AOM and AOM?+?JB groups were administered of AOM (15?mg/kg body weight) subcutaneously once every 2 weeks for 10?weeks. AOM?+?JB and JB groups additionally received JB oil (100??l/kg) orally. At the end of the 16-week experimental period, blood and tissue samples were obtained from the rats following necropsy. The macroscopic findings showed that the application of JB oil significantly decreased adenoma and adenocarcinoma formation both numerically and dimensionally. Immunohistochemically, CEA, COX-2, and Ki-67 immune-expressions decreased, and the immune-expression of caspase-3 increased in AOM?+?JB treated rats. Additionally, JB oil supplementation ameliorated antioxidant defense systems and lipid peroxidation within the colon tissue of AOM?+?JB treated rats. These results reveal that the JB oil acted as a chemopreventive dietary agent, inhibiting cell proliferation and COX-2 expression and inducing apoptosis, resulting in a significant reduction in colon tumor formation.
Eeffect of Low-Level Laser Therapy on Peri-Miniscrew Fluid Prostaglandin E2 and Substance P Levels: a Controlled Clinical Trial
(Aves, 2021) Alkan, Ozer; Kaya, Yesim; Yuksek, Esra; Komuroglu, Ahmet Ufuk
Objective: This study aims to evaluate the effect of low-level laser therapy on peri-miniscrew fluid prostaglandin E2 (PGE2) and substance P (SP) levels during orthodontic treatment. Methods: A total of 15 individuals were included in this study. Miniscrews were inserted to the inter-radicular region of the maxillary right and left second premolar and the first molar teeth, and diode lasers were randomly applied to the right or left side. Irradiation was performed at 940 nm wavelength using a gallium-aluminum-arsenide diode laser with 100 mW power output, 0.125 cm(2) spectral area, 8 J/cm(2) energy density, and 10 seconds of exposure time. Peri-miniscrew fluid samples were collected on the 1st, 3rd, and 7th days, and PGE2 and SP levels were assessed. For statistical comparison, two-way (factors) analysis of variance with repeated measurements on one-factor levels was used at statistical significance (p) of <0.05. Results: PGE2 levels on the 1st, 3rd, and 7th days were 160.64 +/- 10.05, 135.17 +/- 37.18, and 98.57 +/- 22.94, respectively, in the control group and 150.75 +/- 9.08, 87.17 +/- 40.67, and 78.10 +/- 16.50, respectively, in the laser group. SP levels on the 1st, 3rd, and 7th days were 79.90 +/- 12.05, 64.61 +/- 10.05, and 70.05 +/- 9.10, respectively, in the control group and 76.32 +/- 11.39, 60.25 +/- 9.08, and 65.71 +/- 5.59, respectively, in the laser group. The differences in PGE2 and SP levels between the laser and control groups were not statistically significant at all time intervals. Conclusion: Low-level laser therapy cannot be recommended as a clinical adjunct therapy to reduce inflammation and pain around the miniscrews.
Effect of Meloxicam and Flunixin Meglumine on Some Kidney Parameters in Geriatric Male Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2024) Komuroglu, Ahmet Ufuk; Basbugan, Yildiray; Yuksek, Nazmi; Altug, Nuri; Ozdek, Ugur; Dede, Semiha
Advanced age, called geriatrics, negatively affects the aging- related physiological changes on bodysystems. non-steroidal anti- inflammatory drugs (NSAIDs) used in geriatric pets or humans have negative effects on the kidneys. For this purpose, , the effects of Meloxicam and Flunixin Meglumine, which are NSAIDs frequently used in Veterinary Medicine, on the liver and kidney structure and function in geriatric male rats were evaluated. Twenty-four male geriatric rats (30-36 month old) and twenty-four 3-month-old young male Wistar albino rats were used in the study. Six groups were created, with eight rats in each group: young control (YC), young Meloxicam (YM), young Flunixin Meglubine (YFM), geriatric control (GC), geriatric meloxicam (GM), geriatric Flunixin Meglubine (GFM). Control groups (YC and GC) received an intraperitoneal injection of saline using the same volume as in the othergroups. Meloxicam was administered at 5.8 mgkg-1to-1 to the YM and GM groups, and Flunixin Meglumine at 2.5 mgkg-1-1 intraperitoneally to the YFM and GFM groups once a day for 5 days. Neutrophil Gelatinase-Associated Lipocalin (NGAL), Cystatin C (Cyc-c), Kidney Injury Molecule-1 (KIM-1), Interleukin-18 (IL-18), Urea, Creatinine (Crea), Albumin (Alb), and Total Protein (TP) levels, were determined in sera and urine samples. Serum NGAL, Cys-C, and KIM-1 levels in the GC group were found to be significantly higher than those in the YC group (P<0.05). P <0.05). Administration of both NSAIDs caused an increase in serum Cyc-c and NGAL levels in both young and geriatric rats (P<0.05). P <0.05). Since both Meloxicam and Flunixin Meglumine administration caused an increase in NGAL and Cys-c levels in young and geriatric rats, adjusting the drug dose and frequency of administration by evaluating the pretreatment renal function should be considered as a preventive measure.
Effects of Ibuprofen and Low-Level Laser Therapy on Orthodontic Pain by Means of the Analysis of Interleukin 1-Beta and Substance P Levels in the Gingival Crevicular Fluid
(Urban & Vogel, 2021) Kaya, Yesim; Alkan, Ozer; Komuroglu, Ahmet Ufuk; Keskin, Siddik
Objective The goal of this study was to compare the effects of ibuprofen and low-level laser therapy in alleviating orthodontic pain observed after elastomeric separator placement (ESP) by means of the analysis of interleukin 1-beta (IL-1 beta) and substance P (SP) levels in gingival crevicular fluid (GCF) and visual analog scale (VAS). Materials and methods A total of 60 subjects requiring ESP for the banding of maxillary first molars were randomly assigned to the ibuprofen, laser, and control groups. The ibuprofen and control groups received, respectively, 400 mg ibuprofen and placebo lactose tablets orally 1 h before ESP; the laser group received a single low-level laser irradiation session immediately after ESP. GCF samples were collected immediately after ESP (day 0) and on days 1, 3, and 7. Pain intensity was evaluated using the VAS immediately after ESP (baseline) and at hours 2 and 6, as well as on days 1, 3, and 7. Results Although IL-1 beta levels increased significantly on days 1, 3, and 7 compared to day 0, intergroup comparison results revealed insignificant differences. SP levels indicated insignificant within-group differences. Only the SP levels of the ibuprofen group showed a significant decrease on days 0 and 1 compared to the laser and control groups. In all groups, VAS scores increased from baseline to a peak level on day 1, followed by a significant decrease on days 3 and 7. Intergroup comparison results of VAS scores indicated less pain intensity in the ibuprofen group compared to the control group at baseline. Conclusions Only the ibuprofen group exhibited significant decreases in SP levels on days 0 and 1, as well as in VAS scores at baseline.
Effects of Licorice Root (Glycyrrhiza Glabra) Extract on the Livers of Obese Rats
(Taylor & Francis Ltd, 2024) Uyar, Ahmet; Ozdere, Baris; Yaman, Turan; Komuroglu, Ahmet Ufuk
We have investigated anti-obesity effects of the extract of licorice (Glycyrrhiza glabra) root in rats with diet-induced obesity and hyperlipidemia by using histopathological and biochemical methods. Thirty-two Wistar albino rats were divided to four groups of eight: normal control (C), high fat diet (HFD), high fat Diet + Glycyrrhiza glabra (HFD+M), and normal diet with Glycyrrhiza glabra (M). The high fat diet contained 300 g/kg fat (4000 kcal/kg); the daily dosage of Glycyrrhiza glabra extract was 1g/kg body weight by orogastric gavage. Supplementation of Glycyrrhiza glabra extract dramatically reduced increases in body weight caused by the induction of obesity. A hepatoprotective effect of Glycyrrhiza glabra extract was supported by the almost normal histology in the livers of the HFD+M rats, in contrast to the degenerative changes in the HFD rats, which included macrovesicular and microvesicular fat deposits, hydropic degeneration, dilatation of sinusoids and coagulation necrosis of some hepatocytes. Serum levels of alanine transaminase (ALT), aspartic transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol (HDL and LDL) and triglycerides, were ameliorated by Glycyrrhiza glabra extract treatment. We conclude that Glycyrrhiza glabra extract given together with HFD could prevent obesity and reduce liver damage in rats.
Effects of Silymarin on Immunohistochemical Bax and 8-Ohdg Expression, Biochemical Markers and Sperm Parameters in an Experimental Varicocele Model in Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2025) Belhan, Saadet; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Yildirim, Serkan
In this study, the effects of silymarin on immunohistochemical Bcl-2-associatedXprotein (Bax)and8-hydroxy-2'- methoate (DMT e cide is e of t che cals used to otectdeoxyguanosinesomeagriural(8-OHdG) areas m expression, harmful o nisms. biochemical DMT markers and sperm parameters were investigated with an sidues elea ed directly or ind rectly to the envi onm nt experimentally induced varicocelemodelin rats.Thestudy use serious problems in nature DMT resid es mixed wi hthe quat c environmen adversely affect aquat c organisms a d was conducted on 36 Wistar albino rats. The distribution of rats within the group was made in an equal number. Rats in the control group were administered physiological saline daily is effect is carr ed to humans through the food chain. In thisvia oral gavage. In the sham group, an incision was made on udy, oxdative s ress respon es nd ced by DMT pe tic de inontastacusleptodacylus were nvestigated.Forthispurpose, the midline and the renal vein (located on the left) was made xidatvisible.ve A stress probe and was placed antioxidant on thisparametevein. sThe probe Thiobarbituric was coiled acid with the vein but not ligated. In the silymarin group, silymarin active s bstances (TBARS) Glutathione (GSH) Superoxidewas administered by oral gavage at adoseof 75 mg/kg 3 smu ase (SOD) c ta ase CAT) and lutathi ne pe oxidase times a week for 8 weeks. Ligation was performed on rats in PX) caused by dimethoate (DMT) pesticde in P.leptodactylus the varicocele group, unlike the sham group. Varicocele was created inthe varicocele+silymarin groups (50 mg/kg, 75 mg/ 17.5, 35, and 70 mg i-1 concentr tions at 24 and 96 h urs ere investigated Results were determined using EiISA kits. kg). Silymarin application was started 8 weeks after varicocele induction and was applied 3 days a week for 8 weeks. After the analysis, it was seen that sperm parameters were negatively ere obse ved between decreases n CAT and GPx activi ies and affected in the varicocele group. Additionally, severe caspase cr ases in TBARS leve s SPSS 24 0 packa e program one-way 3, 8-OHdG and Bax expressions were detected. Silymarin NOVA (Duncan 05) was used n the evaluation of bio hem cal administration reduced the intensity of expression and had nalyzes As a res lt,t was determined that DMT caused oxidative positive effects on spermatology. These positive effects were even more pronounced with the 75 mg dose. Based on the ress formation in P leptodac ylus and caused changes n enzyme results obtained, silymarin may have the potential to reduce both clinical and pathological symptoms in varicocele cases. tivities.
Evaluation of Glut1, Igf-2, Vegf, Fgf 1, and Angiopoietin 2 in Infantile Hemangioma
(Elsevier France-editions Scientifiques Medicales Elsevier, 2021) Koker, Sultan Aydin; Komuroglu, Ahmet Ufuk; Koksoy, Adem Yasin; Siraz, Ulku Gul; Tekin, Emine; Koker, Alper
Introduction: Infantile hemangioma (IH) is a common vascular tumor in children. It is reported that IHs are associated with immunochemical markers such as vascular endothelial growth factor (VEGF)-A, glucose transporter isoform 1 (GLUT1), and insulin-like growth factor-2 (IGF-2). Material and methods: This cross-sectional study focused on pediatric patients with IH. A total of 46 patients (mean age 14.2 +/- 21.9 months) with IH and 45 healthy controls (mean age 21.8 +/- 15.08 months) were enrolled. Demographic data, clinical findings, and laboratory parameters were recorded. Blood samples were collected. Serum GLUT1, IGF-2, VEGF-A, fibroblast growth factor 1 (FGF1), and angiopoietin 2 levels were assessed by enzyme-linked immunosorbent assay. Results: Serum GLUT1, IGF-2, and VEGF-A levels were significantly higher in patients with IH than in healthy controls (8.80 +/- 4.07 pg/mL vs. 5.66 +/- 4.34 pg/mL, 281.10 +/- 84.12 pg/mL vs. 234.19 +/- 75.38 pg/mL, 1196.99 +/- 389.34 pg/mL vs. 996.99 +/- 349.16 pg/mL, respectively, p = 0.026, p = 0.030, and p = 0.036). Serum GLUT1, IGF-2, and VEGF-A levels in patients with complicated hemangioma were significantly higher than in healthy controls (9.69 +/- 3.94 pg/mL vs. 5.66 +/- 4.34 pg/mL, 289.94 +/- 83.18 pg/mL vs. 234.19 +/- 75.38 pg/mL, 1276.22 +/- 388.24 pg/mL vs. 996.99 +/- 349.16 pg/mL, respectively, p = 0.017, p = 0.022, and p = 0.011). Serum GLUT1, IGF-2, and VEGF-A levels in patients with hemangioma receiving propranolol treatment were significantly higher than in healthy controls. Serum FGF1 levels were higher in patients with IH, complicated hemangioma, and hemangioma receiving propranolol treatment than in healthy controls but the difference was not statistically significantly. Conclusion: Serum GLUT1, IGF-2, and VEGF-A levels were positively correlated with disease severity in patients with hemangioma, for example, in complicated hemangioma and hemangioma requiring propranolol treatment. However, further research on larger and different age subgroups is warranted to assess these markers. (C) 2021 French Society of Pediatrics. Published by Elsevier Masson SAS. All rights reserved.
Evaluation of Serum Ghrelin, Nesfatin-1, Irisin, and Vasoactive Intestinal Peptide Levels in Temporal Lobe Epilepsy Patients With and Without Drug Resistance: a Cross-Sectional Study
(Assoc Medica Brasileira, 2021) Erkec, Ozlem Ergul; Milanlioglu, Aysel; Komuroglu, Ahmet Ufuk; Kara, Mehmet; Huyut, Zubeyir; Keskin, Siddik
OBJECTIVE: Epilepsy is a common disorder that affects the nervous systems of 1% of worldwide population. In epilepsy, one-third of patients are unresponsive to current drug therapies and develop drug-resistant epilepsy. Alterations in ghrelin, nesfatin-1, and irisin levels with epilepsy were reported in previous studies. Vasoactive intestinal peptide is among the most common neuropeptides in the hippocampus, which is the focus of the seizures in temporal lobe epilepsy. However, there is also lack of evidence of whether these four neuropeptide levels are altered with drug resistant temporal lobe epilepsy or not. The aim herein was the evaluation of the serum levels of nesfatin-1, ghrelin, irisin, and Vasoactive intestinal peptide in drug-resistant temporal lobe epilepsy patients and temporal lobe epilepsy (TLE) without drug resistance, and to compare them to healthy controls. METHODS: This cross-sectional study group included 58 temporal lobe epilepsy patients (24 with drug resistant temporal lobe epilepsy and 34 with temporal lobe epilepsy who were not drug-resistant) and 28 healthy subjects. Nesfatin-1, ghrelin, irisin, and Vasoactive intestinal peptide serum levels were determined using enzyme-linked immunosorbent assay. RESULTS: The serum ghrelin levels of patients with drug resistant temporal lobe epilepsy were seen to have significantly decreased when compared to those of the control group (p<0.05). Serum nesfatin-1, vasoactive intestinal peptide, and irisin levels were seen to have decreased in the drug resistant temporal lobe epilepsy group when compared to those of the control and temporal lobe epilepsy groups; however, the difference was non-significant (p>0.05). CONCLUSIONS: The results herein suggested that ghrelin might contribute to the pathophysiology of drug resistant temporal lobe epilepsy. However, further studies are needed to confirm this hypothesis.
Heavy Metal Exposure of Workers Working at Petroleum Products Filling Stations and Its Effects on Oxidative Stress
(Parlar Scientific Publications (p S P), 2020) Atasoy, Nurhayat; Komuroglu, Ahmet Ufuk; Yucel, Ufuk Mercan; Kul, Ali Riza; Gursoy, Tulay
Petroleum products increase oxidative stress and inflammation due to its particulate content. This study aims to determine the levels of heavy metals (zinc, lead, cadmium, chromium) and to measure level of reduced glutathione and enzyme activity of superoxide dismutase in serum and hair specimens of workers (n=50, research group) employed in petroleum products filling stations in Van province. The control group (n=25) was selected working in the office and compared with the research group. Heavy metal levels in hair and serum specimens were determined by using the inductively matched plasma optical emission spectrometry instrument and serum enzyme levels were determined by using spectrophotometric methods. Zinc, lead, cadmium and chromium metal levels were higher in the research group than healthy controls. In addition, reduced glutathione level and superoxide dismutase enzyme activity decreased due to metal exposure and oxidative stress occurred in the research group. Smoking contributed to the exposure of amount of the metals. In research group, positive correlations were found between metal exposures and life span/term of employment. In addition, with the increased life span and term of employment, metal exposure increased and antioxidant enzyme levels decreased. This study shows that workers employed in stations are exposed to heavy metal toxicity and cause oxidative stress.
Influence of Lycopene Administration on Neopterin, Myeloperoxidase and Gamma Glutamyl Transferase in Diabetic Rats
(Parlar Scientific Publications (p S P), 2018) Komuroglu, Ahmet Ufuk; Yur, Fatmagul; Ekin, Ismail Hakki
In this study, it was aimed to investigate the influence of lycopene administration on serum Neopterin (NEO), Myeleperoxidase (MPO) and Gamma glutamil tramsferase (GGT) activity in rats with experimentally induced diabetes. Male Wistar-Albino rats aged 7-8 weeks and weighing 250-300 gr were used in the study. Rats were randomly allocated to four groups as control, lycopene, diabetes and diabetes -lycopene with 7 rats in each. 45 mg/kg streptozotocin (STZ) prepared in cold citrate buffer was applied via intraperitoneal route in order to induce experimental diabetes. Lycopene was prepared in corn oil and administered via peroral route through gavage in the dose of 10 mg/kg daily in lycopene and DL groups. Blood samples were taken into serum tubes from the hearts of the rats under general anesthesia at the end of 28 days of test period. Blood samples were centrifuged and serum was obtained. Neopterin, MPO and GGT activities were determined in serum samples. The lowest neopterin level was detected in control group (p<0.001). The highest neopterin level was obtained in diabetes group, neopterin level of lycopene group was lower than that of diabetes group however a statistically significant difefrence was not detected. Neopterin level of DL group was found lower than that of diabetes and lycopene groups and this decrease was statistically significant (p<0.001). MPO level was found the lowest in diabetes group compared to other groups (p<0.001). MPO level of control group was found statistically significantly higher than that of lycopene and DL group (p<0.001). No statistically significant difference was observed between groups with regard to CRP levels. GGT activity was the highest in diabetes group and the lowest in DL group (p<0.001). In conclusion, inflammation markers, neopterin and GGT were low in the groups which received lycopene. These findings suggest that lycopene may be useful for prevention of the complications of diabetes and related inflammation.
Inhibitory Effect of Stinging Nettle (Urtica Dioica L.) Extract on Body Weight Gain in Rats on a High-Fat Diet
(Termedia Publishing House Ltd, 2024) Kacak, Kenan; Yaman, Turan; Uyar, Ahmet; Komuroglu, Ahmet Ufuk
Introduction: The leaves and seeds of Urtica dioica (UD) are used in folk treatments for many diseases. Anticarcinogenic, anti-inflammatory, antioxidant, and antiallergenic properties of UD have been reported. Aim: To uncover the effects of nettle seed (Urtica dioica; UD) extract on body weight gain in rats on a high -fat diet (HFD). Material and methods: Male Wistar albino rats (n = 32) were divided into 4 groups, comprising a control group, a group that received a HFD (HFD group), a group that received UD extracts (UD group), and a group that received a HFD as well as UD extracts (HFD + UD group). UD extracts were given a daily dose of 300 mg/kg of body weight orally for 75 days. Results: The HFD led to weight gain that was partially moderated by the UD extract. Histopathological findings in the HFD + UD group were uniformly significantly lower than those in the HFD group. Serum alanine transaminase, alanine aminotransferase, triglyceride, and low -density lipoprotein levels were significantly higher in the HFD group than in the HFD + UD group, and the HDL levels were lower in the HFD group than in the control group and the HFD + UD group. Conclusions: The cholesterol levels were discovered to be highest in the HFD + UD group. Therefore, it was concluded that the UD extract did not completely protect the rats against body weight gain.
Investigation of the Effect of Silymarin on Oxidative Dna Damage and Inflammatory Markers in Ischemia/Reperfusion Injury Following Experimental Testicular Torsion/Detorsion in Rats
(Tubitak Scientific & Technological Research Council Turkey, 2021) Belhan, Saadet; Yildirim, Serkan; Kayikci, Caner; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Kuscu, Yagmur
The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats (10-12 weeks of age, weighing 280-300 g) were randomly divided into five groups. Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin (250 mg/kg) group; and torsion 3 h / detorsion 24 h + silymarin (250 mg/kg) group. In the study, 720 degrees torsion was applied to the left testicle. At the end of the study, blood was collected from the rats, and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha (Tnf-alpha) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities decreased, while malondialdehyde (MDA) levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.
Investigation of the Effects of Calcium Fructoborate on Testicular Structure in Rats Within the Framework of Biochemical Parameters, Testosterone Hormone and Dna Damage in Cadmium Chloride Induced Toxicity
(Assoc Pharmaceutical Teachers india, 2021) Belhan, Saadet; Komuroglu, Ahmet Ufuk; Ozdek, Ugur; Mendil, Ali Sefa; Kul, Ali Riza; Dortbudak, Muhammed Bahaeddin; Gezer, Arzu
Background: The present study aims to investigate the effects of calcium fructoborate on testicular DNA damage and testicular tissue biochemical markers and serum testosterone levels after cadmium chloride administration. Materials and Methods: 28 Wistar albino rats (200-220 g) in the study were divided into 4 groups with an equal number. These groups are; Control group (No chemicals applied), calcium fructoborate (100 mg) group, Cadmium chloride (200 mg/L) + calcium fructoborate (100 mg), Cadmium chloride (200 mg/L) group. The study lasted 28 days and both chemicals were applied daily with oral gavage. Results: While 8-hydroxy-2-deoxyguanosine (8-OHdG) expression was moderate in the cadmium chloride + calcium fructoborate group, the expression in the cadmium chloride group was severe. In the cadmium chloride group, testicular tissue glutathione (GSH) and 8-OHdG levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and serum testosterone levels were significantly decreased compared to other groups. It is determined that the reversal of the change in the parameters listed in the calcium fructoborate group. Conclusion: The reversal of the change in the parameters listed in the calcium fructoborate group indicates the positive strength of the present chemical. It is our suggestion to transfer calcium fructoborate into life practice by conducting further clinical studies and evaluating different parameters.
Investigation of the Protective Role of Chrysin Within the Framework of Oxidative and Inflammatory Markers in Experimental Testicular Ischaemia/Reperfusion Injury in Rats
(Wiley, 2020) Belhan, Saadet; Yildirim, Serkan; Karasu, Abdullah; Komuroglu, Ahmet Ufuk; Ozdek, Ugur
This study was performed to evaluate the effect of chrysin on testicular torsion and detorsion damage in rats in terms of biochemistry, histopathology and immunohistochemistry. The study was performed on Wistar albino rats between 250 g and 300 g. A total of 40 rats were used. Five groups were created with eight rats in each group. Group 1 was the control group, and no torsion procedure was performed. In Group 2, 2 hr of torsion and 2 hr of detorsion were applied. In Group 3, 2 hr of torsion and 24 hr of detorsion were applied. In Group 4, 2 hr of torsion, 2 hr of detorsion and 50 mg/kg intraperitoneal chrysin were applied. In Group 5, 2 hr of torsion, 24 hr of detorsion and 50 mg/kg of chrysin were applied. In the torsion/detorsion groups, the study determined decreases in glutathione and testosterone levels, increases in tumour necrosis factor-alpha, interleukin-4, interleukin-6 and interleukin-10 levels, and increases in expression levels of caspase-3 and caspase-8. Chrysin application reduced malondialdehyde, tumour necrosis factor-alpha, caspase-3 and caspase-8 expression levels. We can say that chrysin can be used to reduce damage in cases of testicular ischaemia/reperfusion. For more reliable results, further clinical trials are recommended.
Metagenomic Analysis of Intestinal Microbiota in Florated Rats
(Springernature, 2022) Komuroglu, Ahmet Ufuk; Seckin, Hamdullah; Ertas, Metin; Meydan, Ismet
Changes in gut microbiota have shown that it plays an important role in animal health and metabolic diseases. The intestinal microbiota is a complex structure that functions as an organ system with the presence of trillions of microorganisms. In this study, changes in the intestinal microbiota of Wistar rats with high fluorine were evaluated. Water containing 100 ppm NaF was given to 14 male Wistar albino rats as drinking water for 12 weeks. Fluorine is known to be an inducer of protein oxidation, lipid peroxidation, modulation of intracellular redox homeostasis, and oxidative stress. In this study, it was determined that the level of MDA (molandialdehyde), one of the oxidative stress parameters, increased significantly in the intestinal tissue after fluorine intoxication. The decrease in CAT (catalase) and SOD (superoxide dismutase) enzyme activities was found to be statistically significant. Intestinal tissues were taken under aseptic conditions and microorganisms found in flora were replicated by V3-V4 16S rRNA gene-specific primers. As a result of the sequence analysis, a statistical comparison of the control group and the fluorine applied group was made. The study we have done showed that there was a significant difference in species diversity in the intestinal microbiota of mice treated with fluorine. As a result, the composition of the intestinal microflora, especially Lactobacillus species, was significantly changed in rats with high fluorine.
A New Marker for the Evaluation of Nutrition in Pediatric Critical Care Patients: Zonulin
(Galenos Publ House, 2023) Koker, Alper; Coban, Yasemin; Aydin, Sultan; Tuncer, Gokcen Oz; Akbas, Yilmaz; Kara, Tugce Tural; Komuroglu, Ahmet Ufuk
Objective: Zonulin is a biomarker of increased intestinal permeability. Malnutrition is strongly associated with prolonged length of stay, increased infection, and mortality. This study aimed to determine how the serum zonulin level is affected in patients in PICU, and evaluate the relationship between pre -albumin and Vitamin D. Method: 35 critically ill pediatric patients were included in the study. The control group was formed of 25 healthy children. The albumin, pre -albumin, Vitamin D, and zonulin levels were examined in patients with findings of infection that regressed during ICU follow-up. The differences between prealbumin levels, Vitamin D, and zonulin were analyzed with the Mann -Whitney -U Test. Results: The most common reason for admission to the PICU was respiratory failure in 12 patients. The Vitamin D level was determined to have a mean of 28.8 +/- 12.3ng/mL and <29ng/mL in 9 patients. The albumin level was determined to be mean 3.3 +/- 0.6 mg/dL, and the pre -albumin level was mean 17.8 +/- 7.4mg/dL. The serum zonulin levels in critically ill patients were statistically significantly higher than in the control group. The difference between the zonulin levels of patients with pre -albumin values was statistically significant. The zonulin levels of patients with a Vitamin D value <20 were found to be statistically significantly higher than the zonulin levels of patients with a Vitamin D value >20 Conclusion: The higher zonulin level in critically ill pediatric patients may be associated with prolonged catabolic processes, exposure to oxidative and hypoxic stress, and bacterial translocation development associated with all of these. The results of the current study showed a statistically significant negative correlation between Vitamin D and zonulin levels. Therefore, the relationship between low Vitamin D values and a high zonulin level may be useful in evaluating chronic malnutrition. The serum zonulin level selected as a biomarker for the surveillance and management of nutrition in critically ill pediatric patients is not an appropriate marker.
Protective Effect of Calcium Fructoborate Against Carbon Tetrachloride-Induced Toxicity in Rats
(Springernature, 2023) Aysal, Hatice; Atasoy, Nurhayat; Komuroglu, Ahmet Ufuk
Carbon tetrachloride (CCL4) is a xenbiotic that can cause cellular damage with free radical production. Calcium fructoborate (CFB) is a boron-based nutritional supplement with antioxidant properties. Calcium fructoborate used in our study is marketed by Future Ceutical Corporation as FruiteX-B, which has a chemical structure similar to the natural form of boron found in edible plants. In this study, it was aimed to determine the antioxidant activity, DNA damage, and histopathological effects of CFB on the liver and kidney tissues of rats in the toxicity induced by CCL4. During 14 days of treatment, 42 wistar albino rats were divided into 7 in each group, control group, olive oil (0.25 ml twice a week), CFB (1 mg/day), CFB-CCL4 (1 mg/day, twice a week 0.5 ml), ZY-CFB (0.25 ml/twice a week, 1 mg/2 times day twice), and CCl4 (0.5 ml twice a week). AST, ALT, HDL, LDH, urea, creatinine, triglyceride, total protein and albumin levels were analyzed in the blood serum of rats. The antioxidant defense system enzymes CAT, GR, GPx, SOD activities and GSH, MDA and 8-OHdG levels in liver and kidney tissues were determined and evaluated. In addition, liver and kidney tissues were examined with only hispatological tests. As a result of the findings, it shows that CCl4 disrupts antioxidant defense mechanisms by disrupting some enzyme systems in the kidney and liver. CFB (Fruit-XB), a boronbased dietary supplement, regulates antioxidant metabolism by strengthening biochemical metabolic profiles against oxidation, and also has a protective effect against DNA damage caused by oxidation. Thus, it was concluded that CFB has antioxidant property against CCl4-induced liver and kidney toxicity.
Protective Effect of Silymarin and Gallic Acid Against Cisplatin-Induced Nephrotoxicity and Hepatotoxicity
(Wiley-hindawi, 2022) Dogan, Duygu; Meydan, Ismet; Komuroglu, Ahmet Ufuk
Objective. This study aimed to investigate the effects of gallic acid and silymarin against nephrotoxicity and hepatotoxicity caused by cisplatin. Materials and Methods. In the study, 56 Wistar Albino rats were equally divided into eight groups. Group 1 was the control group; group 2 was the group receiving cisplatin; group 3 was the group receiving cisplatin + gallic acid; group 4 was the group receiving cisplatin + silymarin; group 5 was the group receiving cisplatin + silymarin + gallic acid; group 6 was the group receiving silymarin; group 7 was the group receiving gallic acid; group 8 was the group receiving gallic acid + silymarin. AST, ALT, urea, creatinine, albumin, globulin, and total protein levels were measured at the end of the study. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione (GSH), and 8-hydroxy-2 & PRIME;-deoxyguanosine (8OH-dG) levels were measured in kidney and liver tissues. Additionally, histopathological evaluations of the tissues were also performed. Results. In kidney and liver tissues, cisplatin significantly increased MDA and 8-OHdG levels compared with treatment groups (p < 0.05). Silymarin-treated group significantly increased the SOD activity and GSH amount in the liver tissue compared with the cisplatin-treated group (p < 0.05). Gallic acid significantly increased CAT activity compared with the cisplatin-treated group (p < 0.05). It was determined that the cisplatin-treated group significantly decreased CAT and SOD activity compared with the control group (p > 0.05). Gallic acid showed a significant increase in CAT and SOD activity in kidney tissue compared with the cisplatin-treated group (p < 0.05). Conclusion. As a result, it was observed that gallic acid silymarin had a protective effect on cisplatin-induced nephrotoxic and hepatotoxic effects.