Browsing by Author "Koyun, Hasan"

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Caveolin-1 Polymorphisms in Patients With Severe Obstructive Sleep Apnea
(Taylor & Francis Ltd, 2017) Asker, Selvi; Taspinar, Mehmet; Koyun, Hasan; Ozbay, Bulent; Arisoy, Ahmet
Objective: To investigate the associations of G14713A and T29107A polymorphic variants of Caveolin-1 with severe obstructive sleep apnea (OSA). Materials and methods: This study was performed on 86 severe OSA patients and 86 controls. Genotyping was performed to investigate the association of G14713A and T29107A polymorphisms of Caveolin-1 with severe OSA. Results: The distribution of genotypes of T29107A was significantly different between controls and OSA patients with a higher proportion of TT carriers in the OSA group. Conclusion: T29107A-specific genotype of Caveolin-1 may be linked with severe OSA pathogenesis.
Detection of Growth Hormone (Gh) Gene Polymorphism in Norduz Sheep
(Agricultural Research Communication Centre, 2022) Yousif, Nzar Ibrahim; Koyun, Hasan
Background: The Norduz sheep is a local breed of sheep bred for its meat, milk, progeny and fleece and is one of the genetic resources in the rural areas of Norduz, a district of the Turkish province of Van. The PCR-based method involves amplification of a conserved DNA region and digestion of the PCR products with DNA restriction endonucleases throughout the genome. Based on the resulting polymorphisms, statistical methods can be used to identify the desired genotypes among the alleles found. The objective of this study was to identify the polymorphisms of the growth hormone gene and determine their association with six growth traits in Norduz sheep. Methods: Out of 50 Norduz sheep, three different loci, GHY1, GHY2 and GHY3 (with the length of 599, 690 and 679 bp respectively) of GH gene on chromosome 11 of sheep genome were selected to identify polymorphisms among individuals using the PCR-RFLP technique (GH/Mspl). Result: In this research study, no polymorphisms were detected after digesting three loci of the GH gene with the enzyme Mspl. The 50 individuals of Norduz sheep had only AB genotypes for all three loci, indicating that no polymorphisms were present in the regions of the GH genes studied.
Detection of Putative Loci Affecting Milk Yield in Turkish Awassi Sheep Using Microsatellite Markers
(Springer, 2024) Koncagul, Seyrani; Kiraz, Selahaddin; Koyun, Hasan
On the basis of comparisons between bovine and ovine genome mapping information, the aim of the study was to analyze the genetic diversity of selected DNA microsatellites from the bovine genome and to investigate their correlation with the average daily milk yield in Awassi sheep. 18 informative microsatellite markers were selected from the significant QTL regions affecting milk yield identified in the bovine genome in previous studies. The selected microsatellite markers were then amplified by PCR as reciprocal amplifications on the genomic DNA of Awassi sheep, with standard daily milk yield records. Thus, in this study, 18 microsatellite markers associated with milk yield in the bovine genome were examined for both determination of genetic polymorphism within the flock and the effects of marker loci on average daily milk yield in Awassi sheep. Allele frequencies of markers were determined based on the results of fragment analysis. The analysis of variance showed that the 123 bp allele at the marker locus BMS1341 on BTA2 significantly influenced the average daily milk yield of Ivesi sheep (P < 0.01). On the other hand, the BMS381 locus with a 115 bp allele on BTA2, the MCM140 locus with a 185 bp allele on BTA6, the BMS2721 locus with a 155 bp allele, the BM1237 locus with 174 and 180 bp alleles on BTA7, and finally, the BMS1967 locus with a 117 bp allele, the BM4208 locus with 176 and 182 bp alleles, and the INRA locus with a185 bp allele on BTA8 showed moderately significant effects on the average daily milk yield of Ivesi ewes (P < 0.05).
Genomic Scan To Detect Qtl Using Snp Markers for Simulated Data by Regression Analysis in Half-Sib Design
(Medwell online, 2009) Koyun, Hasan; Koncagul, Seyrani; Okut, Hayrettin
The aim of the present study was to conduct a genome-wide screening for QTL (Quantitative Trait Loci) detection using the simulated phenotype and genotype data sets obtained from the QTL-MAS workshop 12. A genome scan was carried out in 45 half-sib families to identify QTL influencing a hypothetical trait. Among six chromosomes, each chromosome with 1000 SNP loci, I I informative markers at least 2 cM apart from one another were chosen based on PICs with highest chi(2)-statistic. Half-sib data were pooled and used simultaneously in the analyses conducted for each chromosome separately. Data were analyzed by generating an F-statistic every I cM on a linkage map by regression of phenotypes on the probabilities of inheriting an allele from the sire. Permutation tests at chromosome-wide significance thresholds were carried out over 1000 iterations. Among six chromosomes, significant putative QTL were detected on chromosome 1 (27 cM), 2 (36 cM), 3 (18 cM), 4 (0 cM) and 5 (96 cM) across the families (alpha = 0.01 and alpha = 0.05). There was no QTL detected, exceeding chromosome-wide significance level of p<0.05 and p<0.01 on chromosome 6.
Growth Hormone (gh) Gene Polymorphisms in Norduz Sheep
(2018) Mohammed, Rasul Mahmood Mohammed; Koyun, Hasan
Genetik belirteçler ve bunların hayvan moleküler genetiğine uygulamaları yaygın olarak polimorfizmleri belirlemek için kullanılmaktadır. Sınırlı Parça Uzunluğu Polimorfizmi (RFLP), homolog DNA dizilerindeki varyasyonları kullanan bir genetik analiz molekülü yöntemidir ve bilgilendirici genetik belirteçlerden biri olarak kabul edilmektedir. Büyüme hormonu (GH) geni canlının büyüme ve gelişiminde önemli rol oynar Araştırmanın amacı Norduz koyunlarında GH polimorfizmini tespit etmektir. İzole edilen koyun genomik DNA'sında Büyüme hormaonuna etkiyen 422 bp uzunluklu GH1 (Hua ve ark., 2009) geni ve 934 bp uzunluklu GH (Kuulasma, 2002) genleri PCR ile çoğaltılmış ve HaeIII kesim enzimi ile kesilmişlerdir. Buna göre GH1 lokusunda fragmanları 366 bp ve 56 bp olan AA genotipi ve 422 bp ve 366 bp'lik fragmanlara sahip heterozigot AB genotipi Ancak homozigot BB genotipi görülmemiştir. Ayrıca GH lokusu için her üç genotipte AA, AB, BB saptanmıştır. Araştırılan sürü popülasyonun her iki lokus için Hardy-Weinberg dengesinde (HWE) olmadıkları tespit edilmiştir. Bu çalışma da GH1ve GH / HaeIII' nin büyüme karakterleri için koyunlarda genetik bir marker olarak kullanılabileceği sonucuna varmıştır.
Importance and Determination of Body Electric Current Pre and Post Feeding in Turkish Karayaka Sheep
(2024) Bayram, Rıdvan; Çelikyürek, Hasan; Koyun, Hasan
Many scientific studies are conducted directly or indirectly with humans, animals, and plants. We believe that body electricity, which is generated and constantly present in the bodies of living beings, should be considered in scientific studies as an effective factor for production activity. We believe that body electricity should be included in the environment to bring the rumen fluids of sheep used in Daisy II rumen simulators closer to reality. In this way, the most realistic environment is created by adding the influencing factors of body electricity and many factors that can affect the outcome. The study was conducted on a total of 16 Karayaka ewes, including 4 lambs, 4 one-year- old ewes, 4 pregnant ewes and 4 lactating ewes. The data obtained in the study were collected by measuring the body electricity of the animals before and after grazing in 3 different periods for each group. At the end of the study, it was found that the value of body electricity of sheep determined at 0.12±0.001 v (volt) before feeding was higher than the value determined at 0.09±0.002 v after feeding. The difference between the two values was found to be statistically significant (P<0.05). However, it was found that the fact that the live weights of the animals in the groups were different and they were in different physiological periods did not cause a significant (P>0.05) difference in the electrical body currents before and after feeding (except in lambs (P<0.05)). It can be said that the measurements made in other periods and groups can change the electrical body currents after feeding and that the electrical body currents differ according to the animals fed in the barn, especially during the grazing period, before going to pasture and when returning to pasture.
Investigation of Genetic Structure in Van Cats Using Microsatellite Markers
(Elsevier Science Bv, 2017) Koyun, Hasan; Koncagul, Seyrani; Karakus, Kadir; Okut, Hayrettin; Kucuk, Mursel; Yilmaz, Ayhan; Taspinar, Mehmet
A Mitochondrial Dna-Based Molecular Phylogenetics Study of the Mahalli Goat as a New Animal Genetic Resource in Southern Anatolia in Turkey
(Agricultural Research Communication Centre, 2024) Kiraz, Selahaddin; Koncaguel, Seyrani; Vural, Mehmet Emin; Koyun, Hasan
Background: The Karacada & gbreve; Mountains are of extinct volcanic origin and cover a large area in the triangle between Diyarbak & imath;r (Center, Nar and Ergani districts), & Scedil;anl & imath;lurfa (Siverek and Viransehir districts) and Mardin (Derik district). Agricultural activities are limited due to the stony structure of the region; therefore, small animal husbandry is intensively practiced in the villages of the region. Mahalli goats, which are morphologically new domestic goats, are distinguished from other domestic goats by their spiral horn structure. They are not only resistant to cold weather conditions, but also resistant to diseases. Therefore, present study aimed to determine the genetic diversity and phylogenetic relationships of Mahalli goats in Diyarbak & imath;r region. Methods: The study was carried out at & Scedil;anl & imath;urfa, Harran University of Agricultural Faculty, Laboratory of Animal Biotechnology and Molecular Genetics, in 2019-2020. Out of 24 Mahalli goats, forward and reverse primers were used to amplify the 598 bp region over the mtDNA D-loop region encompassing the 481 bp most variable region (HVR1). Analysis of goat D-loop sequences revealed 481 regions of 0.378 G+C, 97 polymorphic regions and 19 haplotypes. The phylogenetic analysis of Mahalli goats was carried out using molecular genetic techniques. Result: As a result of data analysis, both haplotype diversity (HD) and nucleotide diversity (ND) were calculated to be 1.000 +/- 0.0039 and 0.0375 +/- 0.00209, respectively. The genetic distance between the haplotypes of Mahalli goat ranged from 0.003 to 0.087 and the genetic distance between the haplotypes of Mahalli goat and other domestic goat breeds in Turkey ranged from 0.016 to 0.027. All 19 haplotypes used in this study were represented in the lineage A phylogenetic tree.
Polymorphisms of Growth Hormone (gh) Gene and Their Effects on Some Growth Traits in Norduz Sheep
(2020) Yousıf, Nızar Ibrahım; Koyun, Hasan
Genetik veya DNA belirteçleri, bireyler arasındaki genetik varyasyonları ayırt etmesinde önemli birer araçtırlar. Son zamanlarda DNA belirteçler insan, hayvan, su ürünleri yetiştiriciliği ve bitki genomlarında polimorfizmi belirlemek için yaygın olarak kullanılmaktadır. Hayvanlarda polimorfizmi tanımlamak için kullanılabilecek farklı tipte belirteçler vardır, ancak en yaygın olarak kullanılanlar RAPD, RFLP, AFLP ve makro ve microsatellite ile SNPlerdir. Bu çalışmada Norduz koyunlarında Büyüme Hormon geninin (GH) üç farklı lokusunda (GHY1, GHY2 ve GHY3) genetik polimorfizmi(leri) bulmak amaçlandı. Araştırmada, Norduz koyunlarında büyüme hormonu (GH) genlerindeki genetik varyasyonu belirlemek için PCR-RFLP tekniği uygulanmıştır. İlk önce 50 adet Norduz koyunundan genomik DNA saflaştırıldı. Daha sonra GHY1, GHY2 ve GHY3 lokuslarını çoğaltmak için üç primer seti seçildi. Her üç lokus için olası PCR ürün uzunlıkları sırarası ile 599 bç, 690 bç ve 679 bç olarak elde edildi. Ayrıca, üç lokus, bir DNA endonükleaz enzimi olan MspI ile kesildi. Sonuçlar bu çalışmada kullanılan tüm koyunlarda her üç GH lokusu polimorfizminin olmadığını göstermiştir. Bu araştırma altındaki tüm örneklerde sadece AB, heterozigot genotipi gözlenmiş ancak AA ve BB genotipleri saptanmamıştır. Sonuc olarak, gelecekte genomlarda GH polimorfizmini saptamak amaçlı araştırmalar için daha güvenilir bir teknik olan SNP belirteçler önerilir. Anahtar kelimeler: Büyüme hormonu (GH) geni, Genetik belirteçler, Norduz Koyunu, PCR-RFLP.
Relationships Between Growth Characteristics and Gdf9 Gene Polymorphisms in Awassi Sheep
(Taylor & Francis Ltd, 2024) Kiraz, Selahaddin; Koncagul, Seyrani; Koyun, Hasan
The Awassi sheep, a breed with a distinctive fat tail, is bred specifically in the south-eastern region in Turkey. It has proven its adaptability to the hot environment of this region, especially in the provinces of & Scedil;anl & imath;urfa, Hatay and Gaziantep, which are close to the Syrian border. The aim of this study was to investigate the SNP polymorphisms in the GDF9 gene/exon2 using DNA sequencing. In addition, the study investigated the relationships between SNP genotypes and growth traits, in particular birth weight (BW), weaning weight (WW) and weight at the end of the grazing season (GSW), in Awassi sheep. Four different flocks with a total of 131 Awassi lambs were used. PCR was used to amplify exon2 of the GDF9 gene region, which has a length of 815 base pairs (bp), from the genomic DNA of the Awassi sheep. Thus, four new single nucleotide polymorphisms (SNPs) were identified. The results showed that although the effects of SNP2 and SNP4 on body weight (BW) were statistically significant, only SNP4 had a significant effect on weaning weight (WW) (P < 0.05). In addition, SNP1 and SNP3 were found to have no significant effect on any developmental trait in the dataset.
Sheep in Turkey From Wild Types To Domesticated Forms
(2015) Tas, Abuzer; Karakus, Kadir; Comba, Bahat; Koyun, Hasan; Comba, Arzu; Çınaroğlu, Selim
Ülkemiz Ortadoğu ve Avrupa' da yaban hayatı çeşitliliği ile en zengin potansiyele sahip olup, pek çok hayvan türü için gen kaynağı merkezi konumundadır. İnsanının en önemli faktör olarak görüldüğü küresel ısınma, doğal yaşam alanlarının tahrip edilmesi, avlanma nedenleri ile hızla tür, ırk ve gen kaynakları, yani biyoçeşitlilik azalmaktadır. Günümüz evcil koyunların kökenini olumsuz çevre şartlarına dayanıklı ve üreme yeteneği yüksek olarak bilinen Muflon, Arkar ve Argali yabani koyunlar oluşturmaktadır. Ekonomik öneme sahip birçok özelliğinde dolayı koyun, yüzyıllar boyunca evciltme ve buna paralel olarak ıslah çalışmalarına konu olan ilk türlerdendir. Bu süreç içerisinde yabani formda tanımlanan doğal çevreye adaptasyon, türe özgü davranış biçimleri, hayvan refahı ve haklarına ilişkin birçok özelliğin de olumsuz etkilendiğinin söylenmesi yanlış olmayacaktır. Gelecek nesiller için koyun türünün var olması, her şeyden önce olumsuz çevre koşullarına karşın yaşayabilmesi, üremesi için türe ait özelliklerini kaybettirmeden korunması, moleküler düzeyde genomik çalışmaların yapılması ve genotip - çevre ilişkilerinin dikkatle incelenmesi önemlidir
Single Nucleotide Polymorphisms of Gdf9 Gene/Exon 2 Region and Their Associations With Milk Yield and Milk Content Traits in Karakas and Norduz Sheep Breeds
(Tubitak Scientific & Technological Research Council Turkey, 2021) Koyun, Hasan; Kiraz, Selahaddin; Karaca, Serhat; Koncagul, Seyrani; Yilmaz, Ayhan; Karakus, Kadir; Aygun, Turgut
Karakas and Norduz sheep have been adapted to the Lake Van region of Turkey for many years as being domestic genetic resources and meat, milk, and fleece traits are a reliable economic resource especially preferred by small family businesses around the area. In this study, the data set consisted of milk yield (MY) and milk content components; milk fat (MF), fat-free dry matter (FFDM), dry matter (DM), protein (Pro), lactose (Lac), pH, acidity H (aH), and lactic acid (Lac). Besides, Karakas (n = 30) and Norduz (n = 26) sheep were chosen to investigate based on the SNP method. Correspondingly, genomic DNA from both breeds exon 2 of the GDF9 gene region was amplified, 815 base pairs (bp) in length, by means of PCR. Therefore, there were three noval SNPs detected in both breeds under investigation. Although SNP1 and 2 with genotypes of GG and AG had statistically significant impacts on both milk production and milk components (p < 0.01), SNP3 with genotypes of TT, TC had no significant effects on the milk characteristics in question.