Browsing by Author "Mendi, Yesim Yalcin"

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Indirect Somatic Embryogenesis and Synthetic Seed Production in Cyclamen Coum
(Springer, 2025) Kocak, Metin; Mendi, Yesim Yalcin; Yildiz, Mehtap
Cyclamen coum grows naturally in various regions of Turkey, and especially because of its frost hardiness, it interests breeders. However, there is no bred variety of C. coum, and it is not produced for commercial use. In this study, a somatic embryogenesis protocol was developed using leaf and petiole explants from ten C. coum plants that were randomly selected in the north of Turkey. To produce somatic embryos, seven distinct experiments testing explant types, medium level, plant growth regulator (PGR) concentrations, and cold treatment were established. Somatic embryo production was obtained from leaf and petiole explants in semi-Murashige and Skoog medium (MS) supplemented with different concentrations of Naphthalene Acetic Acid (NAA) +Kinetin and NAA +6-benzyladenine (BA). The somatic embryo production rate was provided, ranging between 48.71% and 69.23%. Somatic embryogenesis-derived plantlets were acclimatized to ex-vitro conditions successfully, and 82.5% of them survived. Furthermore, in this research, the somatic embryos were encapsulated with sodium alginate (3% w/v) supplemented with MS, Woody Plant medium (WPM), or Rugini Olive Medium (ROM). The encapsulated somatic embryos tended to develop new somatic embryos with various embryo formation rates instead of directly germinating.
Synthetic Seed Production of Flower Bulbs
(Springer Nature Switzerland Ag, 2019) Kocak, Metin; Sevindik, Basar; Izgu, Tolga; Tutuncu, Mehmet; Mendi, Yesim Yalcin
Flower bulbs are perennial or annual plants with underground structures such as bulb, corn, tuber, and rhizomes. These plants have economic value especially in ornamental plant sector as cut flower, potted flower, and outdoor plants. Most of these plants have garish flower, and many of them are monocotyledon. Cyclamen, Tulipa, Lilium, Narcissus, Gladiolus, Hyacinthus, Crocus, Iris, Allium, Alstroemeria, Anemone, Orchis, Rhododendron, Freesia, Hippeastrum, Muscari, Ornithogalum, Ranunculus, and Zantedeschia are the most important geophytes that are commercially used in the world. These plants can be propagated using conventional and tissue culture techniques. Synthetic seed production is one of these techniques. Synthetic seed, namely, artificial seed, is described as artificially encapsulated plant tissues and somatic embryos with alginate hydrogel. Synthetic seed technology has significant effect on the conservation of the plant tissues and sustainability of the plants. Recently, conservation of the plant species studies significantly increased, and artificial seed method was used as the most common process to conserve important species. In this chapter, oldest and newest synthetic seed production researches were discussed and presented chronologically.