Browsing by Author "Meydan, İ."

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Investigation of Total Flavonoid, Dpph Radical Scavenging, Lipid Peroxidation and Antimicrobial Activity of Allium Schoenoprasum L. Plant Growing in Van Region
(Centenary University, 2020) Koçak, Y.; Oto, G.; Meydan, İ.; Şeçkin, H.
This study, the effect of ethanol extract of Allium schoenoprasum plant was investigated on some antioxidant parameters and pathogenic microorganisms that affect human health. In this coverage, DPPH (1,1-diphenyl-2-picrilhydrazyl) method was used to determine the antioxidant effect of A. schoenoprasum ethanol extract. Lipid peroxidation prevention activity was evaluated by TBA (Thiobarbituric acid) method. Besides, the antimicrobial activity of plant extract on some pathogenic bacteria was investigated via disk diffusion method. Quercetin was used to determine total flavonoid components. According to our findings, the total flavonoid component amount found in 0.2 mg/ml ethanol extract was found equivalent to quercetin with 44.465 ± 0.204 µg/ml. In addition, A. schoenoprasum showed that it was effective compared to BHA (Beta hydroxy acid), which is the positive control of lipid peroxidation activity. While the lowest and highest concentration values of DPPH radical Scavenging activity were 60,125 - 96,285%, these values were found to be 82.178 - 97.357% for BHA. Ethanol extract of A. schoenoprasum, was determined to show antimicrobial activity against some pathogenic microorganisms such as Bacillus subtilis, Escherichiacoli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans. © 2020, Centenary University. All rights reserved.
Metagenomic Analysis of Intestinal Microbiota in Florated Rats
(Humana Press Inc., 2022) Kömüroǧlu, A.U.; Seckin, H.; Ertas, M.; Meydan, İ.
Changes in gut microbiota have shown that it plays an important role in animal health and metabolic diseases. The intestinal microbiota is a complex structure that functions as an organ system with the presence of trillions of microorganisms. In this study, changes in the intestinal microbiota of Wistar rats with high fluorine were evaluated. Water containing 100 ppm NaF was given to 14 male Wistar albino rats as drinking water for 12 weeks. Fluorine is known to be an inducer of protein oxidation, lipid peroxidation, modulation of intracellular redox homeostasis, and oxidative stress. In this study, it was determined that the level of MDA (molandialdehyde), one of the oxidative stress parameters, increased significantly in the intestinal tissue after fluorine intoxication. The decrease in CAT (catalase) and SOD (superoxide dismutase) enzyme activities was found to be statistically significant. Intestinal tissues were taken under aseptic conditions and microorganisms found in flora were replicated by V3-V4 16S rRNA gene-specific primers. As a result of the sequence analysis, a statistical comparison of the control group and the fluorine applied group was made. The study we have done showed that there was a significant difference in species diversity in the intestinal microbiota of mice treated with fluorine. As a result, the composition of the intestinal microflora, especially Lactobacillus species, was significantly changed in rats with high fluorine. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.