Browsing by Author "Meydan, I."

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Arum Italicum Mediated Silver Nanoparticles: Synthesis and Investigation of Some Biochemical Parameters
(Academic Press Inc., 2022) Meydan, I.; Seckin, H.; Burhan, H.; Gür, T.; Tanhaei, B.; Sen, F.
The science world advancing day by day contributes to living systems in many areas with the development of nanotechnology. Besides being easily obtained from plants, the advantages it brings increase the importance of nanotechnology. Environmentally friendly, economical, and compatible with plants are just a few of the advantages it brings. Silver metal is one of the most preferred active ingredients in nanoparticle synthesis. Arum italicum is used in the treatment of various diseases in the health sector due to the structures it contains. In our study, nanoparticle synthesis was made by using Ag metal with Arum italicum plant. Then, the antimicrobial, DNA damage prevention and DPPH radical quenching activity of Ag NPs/Ai nanoparticles were investigated. The interaction of the plant with Ag, analysis by X-ray diffraction (XRD), UV visible spectrophotometer (UV–vis), scanning electron microscope and energy dispersive X-ray (SEM-EDX), Fourier-converted infrared spectroscopy (FT-IR) methods has been done. It has been observed that Ag NPs/Ai clusters formed by Arum italicum with Ag have an antibacterial effect against Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli pathogens. However, an antifungal effect hasn't been observed against Candida albicans fungus. Pseudomonas aeruginosa bacteria exerted a stronger effect than an antibiotic. It is seen that Ag NPs/Ai has a protective and anti-damage effect against DNA damage. The antioxidant effect of Ag NPs/Ai is remarkable when DPPH radical quenching activity is compared to positive control BHA and BHT. © 2021 Elsevier Inc.
Characterisation, Antibacterial and Antioxidant Effects of Mountain Tea (Sideritis L.) Mediated Silver Nanoparticles in Preventing Dna Damage
(Springer, 2025) Gur, T.; Bekmezci, M.; Meydan, I.; Seckin, H.; Sen, F.
Nanoparticle-based products formed by combining atomic and molecular level materials find their place in many fields such as industry, cosmetics, medicine and pharmacy. In our study, we synthesised Ag NPs from mountain tea (Sideritis L.) using green chemistry method and demonstrated their material properties by characterisations such as SEM, EDX, FT-IR, XRD and UV-Vis spectroscopy. It was observed that the obtained silver nanoparticles formed inhibition zone diameters of 18 mm, 10 mm and 10 mm against Bacillussubtilis ATCC 6633, Pseudomonasaeruginosa ATCC 27853 and Staphylococcusaureus ATTC 29213 bacteria, respectively. Candidaalbicans ATTC 90028 pathogen, which we used as a fungus, formed a zone diameter of 10 mm. In addition the effect of Ag NPs against DNA damage was determined by gel electrophoresis method. In the images, it is seen that the damaged DNA in the 2 end well is preserved due to the increase in concentration. The effects of Ag NPs used in the 5th and 6 th wells are much better seen compared to the others. Ag NPs showed strong resistance to possible DNA damage. DPPH method was used to investigate the antioxidant effects of AgNPs produced by green synthesis, which is a biological procedure, and as a result, the radical quenching activity of mountain tea was found to be quite good with a value of 80% compared to the positive control BHA. We hope that Ag NPs synthesised from mountain tea, whose structure and properties have been extensively elucidated by various methods, may have a wide range of uses that can serve humanity.
Characterization of Zn Nanoparticles of Platonus Orientalis Plant, Investigation of Dpph Radical Extinquishing and Antimicrobial Activity
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Bazancir, N.; Meydan, I.
Zinc(Zn) is one of the favorite elements because of its nanostructures and properties. It is known to be involved in many tasks, such as DNA protection, membrane stabilization, protein, ribosome, and carbohydrate production. The aim of our present study is to examine some biochemical parameters directly related to human health as a result of the characterization of the Platonus orientalis plant with Zn. In our study, ZnO particles were obtained with the green synthesis method using Platonus orientalis plant extract and ZnC4H6O4. Scanning electron microscopy was used for SEM and SEM / EDX images of synthesized ZnO NPs. The character ization process was performed using a UV visible spectrophotometer and fourier transformed infrared spectroscopy (FT-IR). As a result of DPPH(2,2-diphenyl-1-picrylhydrazyl) analysis, zinc nanostructures have an important antioxidant potential. The antimicr obial activity of synthesized metal nanoparticles against some pathogens causing disease in humans by the disk diffusion method was investigated. ZnO NPs/Po has been shown to have antibacterial properties against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, and Pseudomonas aeruginosa ATCC 27853. According to the results of the study, the effects of ZnO NPs / Po, we believe that this material will shed light on the scientific world, especially in the field of pharmacology. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Evaluation of Antioxidant, Antibacterial and Thermal Stability Properties of Silver Nanoparticles Synthesised With Infundibulicybe Gibba Extract
(Springer, 2025) Meydan, I.; Seckin, H.; Kocak, Y.; Okumus, E.; Bekmezci, M.; Sen, F.
Mushrooms have been used by people for centuries for various purposes due to their unique taste, high nutritional content and pharmacological effects. Bioactive components in the structure of mushrooms are an important biological source for the green synthesis of silver nanoparticles (AgNPs) as reducing and stabilizing agents. In this study, AgNPs/Ig were synthesized using the mushroom species Infundibulicybe gibba. The color transformation of the formed nanoparticles from yellow to brown occurred and peaked at 370 nm in the UV-Vis spectrum. The nanoparticles had amorphous shape and their molecular characterization was determined by Fourier transform infrared spectroscopy (FTIR). AgNPs/Ig was much more stable (- 50.02 mV) and its hydrodynamic diameter was much lower (216.50 nm) compared to the mushroom extract. A significant increase in the antioxidant activity (IC50 5.66 mg/ml) and thermal stability of the formed nanoparticles was determined. Lipid peroxidation inhibition of the extract and nanoparticles was measured as IC50 value of 6.75 and 5.51 mg/ml, respectively. In the antimicrobial analysis results, while the mushroom extract did not show any inhibition against the selected microorganisms, AgNPs/Ig showed a low antimicrobial activity. As a result, the synthesis of AgNPs/Ig was carried out through green synthesis, which is environmentally friendly, safe, cost-effective, easy to use and does not contain toxic chemicals, and it has been revealed that AgNPs synthesized using this mushroom species have advantageous potential for use.
Investigation of Mycosynthesized Silver Nanoparticles by the Mushroom Pleurotus Eryngii in Biomedical Applications
(Springer, 2023) Kocak, Y.; Meydan, I.; Karahan, T. Gur; Sen, F.
The mycosynthesis of nanoparticles using mushroom is a promising and innovative approach to Biogenic Sustainable Nanotechnology. In this study, mycosynthesis of silver nanoparticles (AgNPs) was carried out using an edible mushroom extract of Pleurotus eryngii (Pe). AgNPs were characterized by ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analyses. UV-vis analysis exhibited an absorption peak at 415 nm, which corresponds to the surface plasmon resonance of AgNPs. FT-IR and XRD revealed the presence of bioactive components and face-centered cubic structure in synthesis. SEM and EDX analyze confirmed that the AgNPs were spherical and 10-45 nm in size, and the presence of strong signals from silver metal. The therapeutic properties of AgNPs were tested by DNA (Deoxyribonucleic acid) damage, antioxidant (2,2 '-diphenyl-1-picrylhydrazyl (DPPH) method), and lipid peroxidation (LPO) assays. Biosynthesized AgNPs were found to restore DNA damage at concentrations of 50 and 75 mu g/mL. The highest DPPH radical scavenging activity of AgNPs was recorded as 89.44% at 400 mu g/mL and was more effective than Pe extract. AgNPs demonstrated potent activity in inhibiting LPO with a percentage of 88.38% at a concentration of 300 mu g/mL. The biological activity of AgNPs was found to be concentration dependent and had more activity than the Pe extract. The obtained results predict that the mycosynthesis method is less toxic, cost-effective and environmentally friendly, and the AgNPs produced will contribute to the development of new-generation drugs in the fields of biomedicine and pharmacology.
Role of Trametes Multicolor in Green Nanotechnology Based Antioxidant, Antimicrobial, Lipid Peroxidation Inhibition From Fungi To Nanoparticles
(Springer, 2024) Kocak, Y.; Okumus, E.; Meydan, I.; Seckin, H.; Bekmezci, M.; Sen, F.
Fungi as source for new-bio based materials has a wide range of potential uses in the pharmaceutical, cosmetic, and medical sectors. They contain abundant natural bioactive chemical resources. This study reports the use of Trametes multicolour (Tm) mushroom extract for the ecofriendly production of silver nanoparticles (AgNPs). The color of the mushroom extract turns dark brown after a certain period of time when combined with metal. (AgNPs/Tm) and then showed maximum absorption at 318 nm, the wavelength of the surface plasmon resonance of AgNPs. The morphology of AgNPs/Tm was spherical and an EDX peak in the 3 keV region indicated the presence of Ag atoms. The binding properties of the biocomponents involved in NPs handling and stabilising the NPs were identified using Fourier transform infrared spectroscopy (FTIR) data. The synthesized NPs exhibited much higher stability (- 37.63 mV), antioxidant activity (IC50 18.92 mg/ml) and inhibition against lipid peroxidation (IC50 7.46 mg/ml) compared to the mushroom extract. Although there was a significant decrease in the hydrodynamic diameter of the NPs, there was an increase in their thermal stability and antimicrobial activity. As a result, the existence and current potential of a new biomaterial suitable for cost-effective and large-scale production in the synthesis of AgNPs has been demonstrated for the first time.