Browsing by Author "Mi̇s, L."

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Evaluation of Oxidant/Antioxidant Markers, Cytokines, DNA Damage and Trace Mineral Levels in Goats Naturally Single and Co-Infected with Theileria Ovis and Anaplasma Ovis
(Alexandria University, Faculty of Veterinary Medicine, 2024) Mi̇s, L.; Deǧer, Y.; Oğuz, B.
The impact of co-infections with Theileria ovis and Anaplasma ovis on caprine health is very limited knowledge and is rarely documented in the literature. Türkiye is endemic for tick-borne diseases (TBDs), posing a risk of co-infections in goats. To evaluate the impact of single and co-infection with T. ovis and A. ovis on caprine health, four groups of goats were examined: healthy goats, goats infected with T. ovis, goats infected with A. ovis and goats co-infected with both species. Sera from blood samples was tested for oxidant/antioxidant markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST)], cytokines [interleukins (IL)-6, IL-1, tumor necrosis factor-α (TNF-α)], DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and trace minerals [(copper (Cu), zinc (Zn), manganese (Mn), selenium (Se)]. MDA, 8-OHdG, TNF-α, IL-1 and IL-6 levels, CAT and GST enzyme activities were found to be significantly increased in both Theileria ovis and Anaplasma ovis single- and co-infections (P<0.05). In goats, single and co-infected with A. ovis showed a statistically significant increase in SOD enzyme activity compared to goats infected only with T. ovis (P<0.05). In goats co-infected with A. ovis and T. ovis, considerable decrease in GPx enzyme activity were observed (P<0.05). Zn and Se levels in single infected with A. ovis indicated a significant decrease. Also, Cu, Zn, and Se levels in co-infected animals decreased significantly (P<0.05). Significant increase in lipid peroxidation and antioxidant enzyme activity in goats with single and double infections indicates impaired erythrocyte membrane integrity due to oxidative stress. Moreover, increased proinflammatory cytokine concentrations in all groups indicate that a high degree of systemic inflammation is induced during parasitaemia. © 2024 Alexandria University, Faculty of Veterinary Medicine. All rights reserved.
Protective Effects of Naringin on Liver Enzymes (Ast, Alt, Alp) and Histopathology in Cyclophosphamide-Induced Rats
(Ataturk Universitesi, 2018) Yaman Bülbül, G.; Mi̇s, L.; Şengül, E.; Yildirim, S.; Çelebi, F.; Çinar, A.
In this study, it is aimed to investigate the protective effects of Naringin on liver enzymes (Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and histopathology in CYP-induced rats. In our study, forty male adult Sprague Dawley rat weighing approximately 200-250 g were used and 5 gropus formed. Analyzes of liver enzymes were done in auto analyzer and spectrophotometer. The liver tissue samples stained with hematoxylin eosin were examined by light microscopy. CYP was found to cause hapatotoxicity and showed increased liver enzymes in rats. AST, ALT and ALP values were 58±11, 32±8, 38±6 in the control group; 237±42, 168±44, 74±11 in the CYP group; 223±33, 158±42, 62±9 in the Naringin 50+CYP group; 117±25, 107±24, 48±8 in Naringin 100+CYP group; 54±9, 31±7, 36±6 in the group of Naringin 100 were found respectively. According to the values obtained from the control group, there is an increase of P<0.001 in all of the AST, ALT and ALP values in CYP-treated groups. Degenerative and necrotic tissue damage caused by CYP in the liver tissue was found to be reduced as dose-dependent with effect of Naringin. In conclusion, different doses of Naringin were determined to be protective effective for CYP-induced hepatotoxicity. © 2018 Ataturk Universitesi.All Rights Reserved.