Browsing by Author "Ozer, Goksel"

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Genetic Diversity Assessment of Promising Walnut (Juglans Regia L.) Genotypes Using RAPD, ISSR, and IPBS Markers
(Sciendo, 2025) Basak, Ibrahim; Muradoglu, Ferhad; Ozer, Goksel; Guler, Emrah
This study investigates the genetic diversity and relationships among different walnut genotypes using three molecular markers: RAPD, ISSR, and iPBS. The RAPD markers produced 23 polymorphic bands, with an average of 7.77 bands per primer and a 75.53% polymorphism rate. ISSR markers generated 22 polymorphic fragments, averaging 8.50 fragments per primer with a 75.65% polymorphism rate. iPBS markers yielded 65 bands, with 72.39% being polymorphic and an average of 11.75 fragments per primer. The PIC values were highest for RAPD markers (average 0.31), followed by ISSR (0.25) and iPBS markers (0.22). RAPD markers also had the highest resolving power (RP) with an average value of 4.93, followed by iPBS (4.80) and ISSR markers (3.90). The diversity parameters, particularly the number of effective alleles, observed heterozygosity, and expected heterozygosity, indicated that the ISSR marker system exhibits the highest genetic diversity among the walnut genotypes. The Mantel test results showed significant correlations among the marker types, especially between iPBS markers and the combined marker analysis, emphasizing the importance of integrating multiple marker systems for a comprehensive assessment of genetic diversity. Clustering and Principal Coordinates Analysis (PCoA) revealed consistent genetic relationships and clustering patterns, with certain genotypes showing close genetic affiliations across all marker systems. This study emphasizes the value of using a combination of molecular markers to gain a thorough understanding of genetic variability in walnut genotypes, essential for conservation and breeding efforts. Future research should continue to use multiple marker systems to further understand the genetic structure and diversity in walnuts and other economically important species. Diese Studie untersucht die genetische Diversit & auml;t und die Beziehungen zwischen verschiedenen Walnuss-Genotypen unter Verwendung von drei molekularen Markersystemen: RAPD, ISSR und iPBS. Die RAPD-Marker erzeugten 23 polymorphe Banden mit einem Durchschnitt von 7,77 Banden pro Primer und einer Polymorphismusrate von 75,53 %. ISSR-Marker generierten 22 polymorphe Fragmente mit durchschnittlich 8,50 Fragmenten pro Primer und einer Polymorphismusrate von 75,65 %. Die iPBS-Marker lieferten 65 Banden, von denen 72,39 % polymorph waren, bei einem Durchschnitt von 11,75 Fragmenten pro Primer. Die PIC-Werte waren bei den RAPD-Markern am h & ouml;chsten (Durchschnitt 0,31), gefolgt von ISSR (0,25) und iPBS-Markern (0,22). Die RAPD-Marker wiesen ebenfalls die h & ouml;chste Aufl & ouml;sungskraft (RP) mit einem Durchschnittswert von 4,93 auf, gefolgt von iPBS (4,80) und ISSR-Markern (3,90). Die Diversit & auml;tsparameter, insbesondere die Anzahl effektiver Allele, die beobachtete Heterozygosit & auml;t und die erwartete Heterozygosit & auml;t, zeigten, dass das ISSR-Markierungssystem die gr & ouml;ss te genetische Diversit & auml;t unter den Walnuss-Genotypen aufweist. Die Ergebnisse des Mantel-Tests ergaben signifikante Korrelationen zwischen den Markertypen, insbesondere zwischen den iPBS-Markern und der kombinierten Markeranalyse, was die Bedeutung der Heranziehung mehrerer Markersysteme f & uuml;r eine umfassende Bewertung der genetischen Diversit & auml;t unterstreicht. Clusteranalysen und Hauptkoordinatenanalysen (PCoA) zeigten konsistente genetische Beziehungen und Clusterungsmuster, wobei bestimmte Genotypen & uuml;ber alle Markersysteme hinweg enge genetische Verwandtschaften aufwiesen. Diese Studie hebt den Wert der kombinierten Anwendung verschiedener molekularer Marker hervor, um ein umfassendes Verst & auml;ndnis der genetischen Variabilit & auml;t bei Walnuss-Genotypen zu erlangen, was f & uuml;r Erhaltungs- und Z & uuml;chtungsprogramme von entscheidender Bedeutung ist. Zuk & uuml;nftige Forschung sollte weiterhin mehrere Markersysteme einsetzen, um die genetische Struktur und Diversit & auml;t bei Waln & uuml;ssen und anderen wirtschaftlich wichtigen Arten besser zu verstehen.
Morphometric Traits and Ipbs Based Molecular Characterizations of Walnut (Juglans Regia L.) Genotypes
(Springer, 2022) Basak, Ibrahim; Ozer, Goksel; Muradoglu, Ferhad
In this study, walnut genotypes that were selected during two growing seasons among thousands of seedlings were analyzed in terms of detailed morphometric, phenological, and chemical traits. A multivariate analysis was conducted with valuable traits for breeding and selection such as morphometric traits, chemical composition, and phenological characteristics. Also, genotypes were characterized by a retrotransposon-based iPBS marker system. The correlation analysis showed significant positive and negative correlations between agro-morphological characters. The principal component analysis explained 71.44% of the total variance into five main components. Principal component and hierarchical cluster analysis divided genotypes into three groups and identified subgroups based on both agro-morphological characters and iPBS marker systems. A high level of polymorphism ratio was observed for tested markers. Mantel's test demonstrated relatively low correlations between molecular and morphological treats (r = 0.04). The genetic similarities among all individuals ranged from 0.39 (between 018 and 015 or 045 genotypes) to 0.98 (between 090 and 094 genotypes) with a mean similarity of 0.67. Remarkable phenotypic and molecular variations were observed among the genotypes. The features of some investigated genotypes were above the acceptable thresholds for walnut selection in breeding programs, and our study indicated that iPBS markers can be beneficial in walnut breeding programs, allowing the evaluation of the genetic relationship between genotypes, helping to differentiate and select the best genotypes to improve agronomic properties.