Browsing by Author "Ozturk, Cihat"

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Determination of Mic Values of Various Antimicrobial Agents and Presence of Resistance Genes in Pasteurella Multocida Strains Isolated From Bovine
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Gulaydin, Ozgul; Gurturk, Kemal; Ekin, Ismail Hakki; Ozturk, Cihat
Pasteurella multocida is an important bacterium that can cause respiratory infections in cattle. Due to the usage of antimicrobial agents in the treatment of the disease frequently, it is critical to follow the antimicrobial susceptibility of the isolates. In this study, minimal inhibitory concentrations (MIC) of various antimicrobial agents and presence of genes related to resistance were investigated in 59 P. multocida strains isolated from the respiratory tract of cattle. According to MIC values determined by E-test, all of the isolates were susceptible to enrofloxacin, chloramphenicol and gentamicin, but resistant to cefoxitin. In addition, high resistance to ampicillin (88.14%), tilmicosin (64.41%), clindamycin (83.05%) and streptomycin (59.32%) were observed in the isolates. When the resistance genes were examined by PCR, it was determined that blaROB-1, tet H, sul II, str A/aphA 1 and erm 42 genes could play an important role in penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglycoside and macrolide resistance, respectively. It was concluded that the usage of ampicillin, tetracycline, sulfamethoxazole + trimethoprime, macrolide and aminoglycosides should be considered for the treatment of respiratory tract infections caused by P. multocida in cattle. Also, it was determined that antimicrobial resistance genes could play an important role in the development of resistance in P. multocida.
Investigation of Selected Bacterial Agents Causing Sheep Abortion in the Van Province by Rt-Pcr and Histopathological Methods
(veterinarni A Farmaceuticka Univerzita Brno, 2023) Gulaydin, Ozgul; Ozturk, Cihat; Ekin, Ismail Hakki; Ilhan, Ziya; Ilhan, Fatma
Abortion causes significant economic losses in the sheep industry. Determination of the aetiology is important in dealing with abortions. The present study was aimed to identify selected important bacterial pathogens in the abortion cases of sheep. A total of 113 samples (105 aborted sheep foetuses, 4 placentas, and 4 vaginal swab samples) from 85 different sheep flocks were examined by real-time PCR (RT-PCR) regarding Chlamydia (C.) spp., C. abortus, Brucella (B.) spp., B. melitensis, Salmonella (S.) spp., S. Abortusovis, Coxiella (C.) burnetii, Listeria (L.) spp., L. monocytogenes, and Campylobacter spp. All cases that were found to be positive for bacterial agents by RT-PCR, were examined pathologically. Tissue samples of foetuses that were found to be positive for B. melitensis and L. monocytogenes by RT-PCR were also investigated immunohistochemically. A total of 35 (30.9%) samples were found to be positive by RT-PCR, with 15 (42.8%), 9 (25.7%), 5 (14.2%), 4 (11.4%), 1 (2.8%), and 1 (2.8%) of them being identified as C. abortus, B. melitensis, S. Abortusovis, C. burnetii, L. monocytogenes and Campylobacter spp., respectively. The presence of the antigen was confirmed also immunohistochemically in the cases with B. melitensis and L. monocytogenes. As a consequence, C. abortus was found to cause the highest rate of sheep abortion cases, which should be taken into account when implementing control measures in epidemiological investigations.
The Investigation of The Presence of Some Bacterial and Viral Agents in Pearl Mullet (Chalcalburnus Tarichii, Pallas 1811) by Real-Time Pcr and The Histopathological Examination
(Parlar Scientific Publications (p S P), 2018) Gulaydin, Ozgul; Ozturk, Cihat; Onalan, Sukru; Karapinar, Zeynep; Arabaci, Muhammed; Ekin, Ismail Hakki; Ilhan, Fatma
The aim of this study was to identify some bacterial (Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae) and viral (infectious pancreatic necrosis virus, viral hemorrhagical septicemia virus and infectious hematopoietic virus) pathogens by comparing the bacteriological culture and molecular methods with histopathological examination of various tissues seen in Pearl mullet from freshwater rivers inflowing to the Van lake. Accordingly, bacterial and viral agents were detected in a total of 180 pearl mullet samples by bacteriological culture and Real-Time PCR methods. The samples were collected from six different freshwater rivers that flows to Van lake by random sampling. In the study, Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Infectious Pancreatic Necrosis Virus, Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus could not be isolated. However, Aeromonas spp. was identified by bacteriological culture and Real Time PCR methods at 22.22% and 53.33% respectively. The most dominant species was observed to be Aeromonas hydrophila among all identified Aeromonas spp. In histopathological examinations of Aeromonas spp. positive samples, disorders were detected in gill, liver, spleen and kidney tissues. Telangiectasia, edema, hyperplasia and adhesions were observed on the seconder laminates of gills. In liver, the order of the Remak cords were disarranged, and degenerative changes formed in hepatocytes. Melano-macrophages loaded with hemosiderin were intensively detected in all Aeromonas positive samples. As a result, the risk of motile Aeromonas infections in the pearl mullet living in freshwaters flowing into the Van lake is still highly and this could lead to serious adverse effects on human health. To prevent these effects, attention must be paid to hygiene conditions and cold chain in fish served for consumption, the and rivers must be protected from pollution.
Phylogenetic Analysis of Black Queen Cell Virus and Deformed Wing Virus in Honeybee Colonies Infected by Mites in Van, Eastern Turkey
(Polish Soc veterinary Sciences Editorial office, 2018) Karapinar, Zeynep; Oguz, Bekir; Dincer, Ender; Ozturk, Cihat
This study aimed to determine the presence and prevalence of viral and parasitic infections causing high rates of colony loss in honey bee colonies in Van province, eastern Turkey. Twenty-six different apiaries were collected from five counties in Van province. These samples were tested by Reverse-Transcriptase PCR (RT-PCR) for acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), black queen cell virus (BQCV) and deformed wing virus (DWV). Selected positives were sequenced, phylogenetically analyzed and investigated in terms of Varroa. DWV and BQCV were identified in 69.23% (18/26) and 88.46% (23/26) of the bees respectively whereas ABPV and CBPV were not detected in the sampled apiaries. Results of the phylogenetic analysis of DWV and BQCV sequences showed 94-100% similarity to DWV and BQCV isolates obtained from Genbank. Prevalence of varroasis was 89% (23/26) in Van. The obtained samples were identified as Varroa destructor by morphological investigation. The study showed that viral and parasitic agents commonly infect honeybees in Van province, with high prevalence rates for BQCV and DWV. There was also a high degree of conservation of DWV and BQCV sequences distinct from DWV and BQCV isolates from other geographical regions. These findings, including current prevalence and phylogenetic analysis data for DWV, BQCV and varroazis in honeybees, are useful for future studies.