Browsing by Author "Purisa, S."

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Circulating Levels of Vegf Family and Their Receptors in Hepatocellular Carcinoma
(2010) Kemik, O.; Sumer, A.; Sarbay Kemik, A.; Purisa, S.; Tuzun, S.
To analyze the serum levels and prognostic significance of vascular endothelial growth factor (VEGF) family (VEGF-A, -C and -D) and their receptors (VEGFR-1 and -2) in patients with hepatocellular carcinoma. The serum levels of VEGF family members were measured in 56 control subjects and 68 patients with hepatocellular carcinoma using an enzyme-linked immunosorbent assay (ELISA). These measurements were correlated with clinic pathological features. The serum levels of VEGF-A and its receptor, VEGFR-1, were significantly higher in patients with hepatocellular carcinoma than in controls (p<0.001). There was no significant difference in serum levels of VEGF-C and VEGFR-2 between patients and controls. VEGF-A levels were associated with advanced tumor stage and presence of metastases. VEGFR-1 was associated with metastases, advanced overall stage, tumor differentiation. VEGFR-2 levels were associated with poor tumor differentiation. Serum VEGF levels are significantly in the same cohort of patients with variable clinic pathological features and prognostic values. The measurement of VEGF-A and its VEGFR-1 levels in sera may reflect the development of tumor, metastasis and angiogenesis (Tab. 2, Ref. 20). Full Text in free PDF www.bmj.sk.
Human Neutrophil Peptides 1, 2 and 3 (Hnp 1-3): Elevated Serum Levels in Colorectal Cancer and Novel Marker of Lymphatic and Hepatic Metastasis
(Sage Publications Ltd, 2013) Kemik, O.; Kemik, A. S.; Sumer, A.; Begenik, H.; Purisa, S.; Tuzun, S.
Objective. Biological levels of localized colon tumors are needed for the diagnosis and prevention of colon cancer. Numerous biomarker studies are produced currently through research. We investigated some peptides with the aim of identifying biomarkers for colorectal cancer and lymphatic and hepatic metastasis. Materials and methods. A total of 100 patients with histologically proven colorectal cancer and 60 normal volunteers were included in this study. Preoperative serum was collected for analysis. Human neutrophil peptides levels (HNP) were assayed by solid phase RIA (Radio Immun Assay) based on monoclonal antibodies. Results. The HNP-1, -2 and -3-also known as alpha-defensin-1, -2 and -3-are present in elevated concentrations in the plasma from colorectal cancer patients. Conclusion. Preoperative high plasma HNP 1-3 levels are associated with colorectal cancer. The HNP 1-3 levels may procure information on patients with lymph node or hepatic metastasis.
A Novel Screening Test for Colon Cancer: Talin-1
(verduci Publisher, 2014) Bostanci, O.; Kemik, O.; Kemik, A.; Battal, M.; Demir, U.; Purisa, S.; Mihmanli, M.
OBJECTIVE: Cell adhesion and angiogenesis within the extracellular matrix involve special signaling molecules, such as integrins and the actin binding protein Talin-1. The aim of this study was to investigate and describe the expression of Talin-1 for the early detection of colon cancer. PATIENTS AND METHODS: Blood serum samples were collected from 50 healthy volunteers and from 90 patients with colon cancer. Using an enzyme-linked immunosorbent assay (ELISA), all 140 samples were analyzed. RESULTS: Preoperative levels of Talin-1 in the serum were significantly higher in patients with colon cancer (p < 0.001). No significant correlation was found between preoperative levels of Talin-1 in the serum and the age and gender of the patients (p < 0.05). However, a significant correlation was found between Talin-1 levels and the tumor grade, TNM stage, and lymph node metastasis (p < 0.001). CONCLUSIONS: Talin-1 may play a role in the reinforcement of cell proliferation, cell adhesion, and angiogenesis in colon cancer. Thus, the Talin-1 protein activity may be a novel biomarker to detect colon cancer in humans.
Relationship Between Clinicopathologic Variables and Serum and Tissue Levels of Dickkopf-1 in Patients With Rectal Cancer
(BMJ Publishing Group, 2011) Kemik, O.; Kemik, A.S.; Sumer, A.; Begenik, H.; Purisa, S.; Tuzun, S.; Kotan, C.
The major biomarker for rectal cancer is the pathologic development of the tumor. In our study, we identified Dikkopf-1 (DKK1) as a novel biomarker and a therapeutic target for rectal cancer. To emphasize the biological and clinicopathologic significance, we performed tumor tissue and serum analysis of 150 rectal cancer samples with enzymelinked immunosorbent assay. Serum DKK-1 levels are found significantly higher in controls, in poor differentiation, and depth of invasion (in pT3 and pT4), present lymph node metastasis, and TNM stage (in pT3 and pT4) according to good differentiation, depth of invasion (in pT1 and pT2), absent lymph node metastasis, and TNM stage (in pT1 and pT2; P < 0.001 and P < 0.0001, respectively). Tissue DKK-1 levels are found in patients with rectal cancer than in control tissues (P < 0.0001). Dikkopf-1 correlated significantly with depth of invasion (P = 0.009), lymph node metastasis (P = 0.028), venous involvement (P = 0.019), and advanced pTNM stage (P = 0.001). There was no correlation between DKK-1 and age or sex (P > 0.05). This marker is also a potential candidate for development of rectal cancer cells and cancer progression. Copyright © 2011 by The American Federation for Medical Research.
Serum Des-Γ in Patients With Pancreatic Head Adenocarcinoma
(Comenius Univ, 2011) Kemik, A. S.; Kemik, O.; Purisa, S.; Tuzun, S.
The total des-gamma-carboxyprothrombin (DCP) produced in pancreatic adenocarcinoma and the correlation between the tissue DCP and serum DCP levels has been examined. Fifty patients with resectable pancreatic head cancer were examined regarding both the tissue and serum levels of DCP. The mean tissue DCP in the cancer tissue, non-cancer tissue, the serum levels in patients, and the serum levels in controls was 190.500 mAU/ml mg protein, 32.000 mAU/ml mg protein, and 46.000 mAU, and 10.000 mAU, respectively. The elevation of tissue and serum levels of DCP in pancreatic adenocarcinoma patients is a very useful marker for pancreatic adnocarcinoma and should be used without any other tumor marker (Tab. 2, Ref. 15). Full Text in free PDF www.bmj.sk.
Serum Level of Soluble Vascular Adhesion Molecule 1 in Patients With Rectal Cancer
(TIP ARASTIRMALARI DERNEGI, 2011) Kemik, O.; Kemik, A.S.; Hasirci, I.; Adaş, M.; Purisa, S.; Dülger, A.C.; Tüzün, S.
Aim: Vascular adhesion molecule 1 (VCAM-1) plays an important role in solid tumor enlargement and/or metastases. This study evaluated the clinical significance of measuring serum levels of soluble VCAM-1 in rectal cancer and aimed to clarify the biologic significance of its local expression. Method: Serum was collected from 90 patients with rectal cancer and 40 healthy volunteers. Cancer tissue was collected from 84 patients. The level of soluble VCAM-1 in serum and cancer tissue was measured enzyme linked immunosorbent assay.Result: The mean soluble VCAM-1 level in patients was significantly higher than that in control subjects. Elevated serum soluble VCAM-1 was significantly associated with clinicopathologic paramaters such as tumor size, lymph node metastasis, distant metastasis, and poor prognosis. The prognosis for stage 2 patients positive for soluble VCAM-1 level was comparable to that for stage 3 patients. In addition, the serum level of soluble VCAM-1 level was not found correlation with the cancer tissue level. Conclusion: The preoperative level of soluble VCAM-1 level reflected disease progression and was a sensitive biomarker for rectal cancer.
Serum Thymidine Kinase Is Associated With Gastric Adenocarcinoma
(2011) Kemik, O.; Kemik, A.S.; Purisa, S.; Tuzun, S.
Thymidine kinase (TK) plays an essential role in the processing of thymidine within the cell and therefore it is an important marker of proliferation, particularly in tumor cells. The aim of our study was to determine the diagnostic and prognostic importance of TK measurement in gastric adenocarcinoma. The serum level of TK was measured in 72 patients with gastric adenocarcinoma. Serum levels of TK were significantly elevated in gastric adenocarcinoma in comparison with healthy individuals. Also, we have found that there are significant variations in TK serum concentrations when measured that correlate with important variations in clinico-pathological features and prognostic significance.
Transforming Growth Factor Beta-1 in Human Colorectal Cancer Patients
(TIP ARASTIRMALARI DERNEGI, 2011) Kemik, O.; Kemik, A.S.; Purisa, S.; Hasirci, I.; Dülger, A.C.; Adaş, M.; Tüzün, S.
Aim: Colorectal cancer patients are treated with surgery and sometimes radiotherapy and chemotherapy. Transforming growth factor beta-1 (TGF-β1) acts both as an inhibitor of tumor growth and as a promoter of tumor progression. The aim of this study was to determine the levels of TGF-β1 in plasma in colorectal cancer patients and relate these to the effect of clinicopathological variables. Method: One hundred patients scheduled for colorectal cancer surgery were included. Blood samples were taken during surgery and later assayed with enzyme linked immunsorbent assay for total TGF-β1 and active TGF-β1. Result: Total and active TGF-β1 was higher in tumor samples compared to controls (p<0.001).Total TGF-β1 was higher in patients with metastases compared to patients without. Active TGF-β1 levels were not found statistically different in patients with metastases. Conclusion: Higher levels of total TGF-β1 in plasma at surgery may be indicate of distant metastases, Measurement of total TGF-β1 in colorectal cancer patients may be of clinical use in the future.