Browsing by Author "Rağbetli, M.Ç."

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Effects of Diclofenac Sodium Exposure During Pregnancy on Cox-2 Expression in the Ductus Epididymis and Ductus Deferens of the Offspring Rat
(Ondokuz Mayis Universitesi, 2024) Kuşcu, O.; Altindağ, F.; Aslan, R.; Rağbetli, M.Ç.
The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis (DE) and ductus deferens (DD) of the offspring rat. 8 Eight adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham (S) and diclofenac sodium (DS). It was injected into the S group with 1 ml/kg/day saline for 10 days, and it was injected into the DS group with 6.1 mg/kg/day DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis (CPE) and severe COX-2 in the cauda epididymis (CE), while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis (COE) of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system. © 2024 Ondokuz Mayis Universitesi. All rights reserved.
The Effects of Streptozotocin on Spinal Cord Motor Neurons Count in Rat: a Stereological Study
(Yuzuncu Yil Universitesi Tip Fakultesi, 2019) Rahım, O.O.; Çölçimen, N.; Rağbetli, M.Ç.
In this study we investigated effects of Streptozotocin (STZ) administered at different doses on the total number of neurons in segment Thoracic 11 (T11) of the spinal cord of adult rats by using stereological methods. Twenty-eight adult male Wistar albino rats were used. The rats were divided into four groups; control group, saline group (0.5 ml of 0.9% NaCl i.p.), 45mg/kg of STZ (i.p.) group and 65 mg/kg of STZ (i.p) exposed group. At the seventh day, the rats were perfused with 10% neutral buffered formaldehyde. T11, which is one of the transverse spinal cord segments, was isolated and processed for routine histologic examination. Semithin sections (0.5µm thick) were obtained using a ultramicrotome according to systematic random sampling strategies. The sections were stained with Toluidine Blue with Borax. The motor neurons were counted unbiased stereological disector/Cavalieri combination method. The groups were compared statistically. In terms of motor neuron number, no significant difference among the groups was found (p>0.05). Our results indicated that exposure to different doses of Streptozotocin has no effect on the T11 segment of the spinal cord total motor neuron number of the adult rats. © 2019, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of the Effects of Diclofenac Sodium in Rat Ovary on the Number of Preantral Follicles by Stereological Methods in Prenatal Period
(Yuzuncu Yil Universitesi Tip Fakultesi, 2017) Çölçimen, N.; Rağbetli, M.Ç.; Kara, M.; Arıhan, O.; Akyol, V.
For healthy future generations pregnancy is a period in which maximum amount of attention should be given. In this period, choice, duration and dosage of medication as well as the changing physiology of the mother should be taken into account. Non-steroidal anti-inflammatory drugs (NSAIDs) are a group of drugs that have analgesic, anti-pyretic and antiinflammatory effects. This group of drugs can be used in the treatment of the ongoing rheumatic diseases or which occur during the pregnancy period and the complications that occur due to pregnancy. We aimed to show how it affects the number of ovarium preantral follicles of newborn fetus on rats by using Diclofenac Sodium which belongs to this drug family group. Diclofenac Sodium (1 mg/ kg, IM) was injected for 15 days from the 5th day of pregnancy to pregnant rats in Diclofenac Sodium group and saline (1 ml/ kg, IM) was injected to the pregnant rats of the sham group at the same time period. In the 4th week after birth, ovarian tissue preparates of rat pups were stained with hematoxylin-eosin and ovarian total tissue volumes and preantral follicle counts were evaluated under light microscope. There was no statistically significant difference between groups for ovarium total tissue volumes and preantral follicle numbers (p>0.05). Lowest value of follicular intensity was observed in Diclofenac sodium group among study groups however no statistically important difference was found (p>0.05). Results obtained in our study may be related with administration of drug in not a high dosage (1mg/kg). Further studies may be performed with higher doses. © 2017, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.