Browsing by Author "Sensoy, S."

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Comparative Adaptation Responses of Melon (Cucumis Melo L.) Genotypes To Salinity Stress
(Tarbiat Modares Univ, 2021) Erdinc, C.; Inal, B.; Erez, E.; Ekincialp, A.; Sensoy, S.
The objective of this work was to understand the mechanisms of physiological, biochemical, and molecular responses to salinity stress of three Turkish melon genotypes (YYU 1, YYU 4 and CU 196) and cv. Ananas. The study used Randomized Complete Block Design (RCBD) and pots were irrigated with Hoagland nutrient solution after two-leaf stage until harvesting by 50 and 75 mM NaCl concentrations. For evaluation of responses, chlorophyll and carotenoid content, total phenolic and flavonoid amount, proline variations, and nutrient elements were determined. Moreover, qRT-PCR analyses were performed to identify the expression level of six TF (Transcription Factors) genes (WRKY24, TCP15, CmHD-Zip, mTERF2, Dof3 and CmADH2). Increase in salt application led to increase in chlorophyll content in the melon genotypes, but decrease (about 55%) in cv. Ananas. Phenolic, flavonoid, and proline contents varied based on the melon genotypes, but generally increased in Ananas. Expression levels of TCP15 and WRKY24 showed more fold change at 75 mM NaCl treatment. On the other hand, the expression of CmADH2 and Dof3 showed more fold change at 50 mM NaCl treatment. Finally, according to adaptation mechanisms of melon genotypes, the study might help in selection and detection of the salt tolerant ones.
Determination of Genetic Diversity Among Cumra Melon Genotypes by Issr Markers
(Cukurova Univ, Fac Agriculture, 2012) Turkmen, O.; Sensoy, S.; Erdinc, C.; Paksoy, M.
The present study aimed to determine genetic relationships among 32 melon genotypes collected from Cumra-Konya by molecular markers. Thirty-six polymorphic ISSR markers obtained from 10 primers were used to define the genetic similarity among the melon genotypes by dendrograms or two and three dimensional scaling obtained from Simple Matching distance matrix. The computer program POPGENE was also used to calculate the statistical measures of genetic variation (Nei's gene diversity (H), Shannon's information index (I), and percentage of polymorphic loci). Based on the molecular Simple Matching distance matrix, the most similar genotypes were C15-C22 followed by C23-C26. Of all evaluated genotypes, the most distinct one was C28. The genetic variation estimates for melon genotypes in the present study were high (H = 0.27, I = 0.43 and 100 % polym).
Determination of Genetic Diversity in Some Local Alfalfa (Medicago Sativa L.) Ecotypes Using Ssr Markers
(Pakistan Agricultural Scientists Forum, 2021) Ertus, M. M.; Sensoy, S.
In this study, the genetic diversity and relationships were examined by using SSR markers among six local alfalfa (Medicago sativa L.) ecotypes grown in Eastern Anatolian Region of Turkey. Eleven SSR primers produced 111 polymorphic bands in 90 alfalfa genotypes (15 genotypes from each ecotypes). The maximum (15) and minimum (5) allele numbers were obtained from the primers AFcat32 and AFct11, respectively. The polymorphism rate among the ecotypes ranged from 54.55% to 74.03%. According to the similarity coefficient matrix used to determine the degree of relationship, the closest similarity (0.68) was determined between the ecotypes Catak and Ercis, but the most distant one (0.37) was determined between the ecotypes Gurpmar and Muradiye. From the ecotypes, the genotypes closest to each other (0.88) were found to be in the population of Gurpinar, but the genotypes farthest to each other (0.41) were found to be in the population of Ercis. Based on Nei genetic diversity, the highest genetic diversity was found in the population of Ercis, while the lowest one was obtained in the population of Gurpinar. Moreover, no very closely related genotypes were detected in the studied ecotypes having high genetic diversity.
Determination of the Anthracnose (Colletotrichum Lindemuthianum (Sacc. and Magn.) Lambs. Scrib.) Resistance in Some Turkish Bean Genotypes by Artificial Inoculation and Molecular Methods
(Pakistan Agricultural Scientists Forum, 2017) Erdinc, C.; Turkmen, O.; Demir, S.; Sensoy, S.
A total of 123 bean genotypes collected from different regions of Turkey and 7 foreign anthracnose-resistant varieties were evaluated for resistance to anthracnose disease caused by the fungal pathogen Colletotrichum lindemuthianum (Sacc. and Magn.) Lambs. Scrib. Analysis was conducted using artificial inoculation as well as resistance-linked molecular markers. Artificial inoculation was performed in a growth chamber using Race 55. Molecular markers were obtained from SCAR [SAS13 (950 bp, Co-4(2)), SC08 (910 bp, Co-4), SF10 (1072 bp, Co-10), SZ04 (567 bp, Co-6)] and RAPD (OA18(1500) (1500 bp, Co-1(5)) primers associated with resistant genes. Results of artificial inoculation showed that in addition to the 7 foreign varieties, 21 Turkish bean genotypes were anthracnose-resistant, while the remaining 102 Turkish genotypes were not. Moreover, results of molecular-marker screening indicated the presence of one or more amplicons associated with resistant-gene markers (Co-4(2), Co-4, Co-6, Co-10 and Co-1(5)) in the majority of resistant genotypes. Only one accession (G89) had all 5 amplicons, and 6 accessions (G19, G20, G34, G93, G97 and Jaguar) had no amplicons. The present study discovered Turkish bean germplasm of both Andean and Mesoamerican source to have a high level of resistance against anthracnose Race 55. In addition, amplicons connected with several resistance genes were found in this broad bean germplasm. Bean genotypes distinguished in the present study as anthracnose resistant could be utilized in future reproducing programs.
Effects of Drought Stress on Plant Growth Parameters, Membrane Damage Index and Nutrient Content in Common Bean Genotypes
(Pakistan Agricultural Scientists Forum, 2017) Kabay, T.; Erdinc, C.; Sensoy, S.
Common bean (Phaseolus vulgaris L.) is an important crop in both Turkey and the world. Drought is one of the important abiotic stress factors limiting the quality and quantity of common bean. This study was conducted to determine the drought-tolerant and susceptible common bean genotypes in Lake Van Basin of Turkey. Total 48 bean genotypes consisted of 40 genotypes from Lake Van Basin, 6 from different parts of Turkey and 1 drought susceptible varietyZulbiye and 1 drought tolerant variety-Yakutiye. The drought experiment was designed as completely randomized plot with 4 replicates in plastic greenhouse conditions. The four-week-old bean seedlings were subjected to drought stress by completely stopping irrigation for 8 days compared to control seedlings. The investigated traits were growth rate, fresh and dry shoot weights, fresh and dry root weights, stem diameter, area and number of leaves, leaf relative water content (LRWC), membrane damage index (MDI), and ion contents (K, Ca and Na) beside the 0-5 visual scale assessment of drought. At the end of the study, it was determined that plant growth and development of the bean genotypes were negatively affected by drought; however, the studied bean genotypes reacted variously to this stress factors. The genotypes V7, V15, V33, V82, V89 and V-A1 were found to be drought sensitive as the variety Yakutiye, while the genotypes V21, V62, V69, V71, V86, V95 and T7 were found to be drought tolerant as the variety Zulbiye.
Effects of Endophytic Bacteria on Some Physiological Traits and Nutrient Contents in Pepper Seedlings Under Drought Stress
(Centenary University, 2021) Sadak, A.; Akkopru, A.; Sensoy, S.
The present study was conducted to determine the effects of the endophytic bacteria (EB) on some physiological traits and nutrient contents in pepper (Capsicum annuum L.) seedlings grown under drought stress. The pepper cv. Mostar F1 and two EB isolates [Bacillus thurigiensis (CA41/1) and Ochrobactrum sp. (CB36/1)] were employed under drought stress condition. The first EB application was at a density of 108 CFU mL-1 as 10 mL plant-1 at the first cotyledon leaf stage and the second one was two weeks later. The seedlings were irrigated by gravimetric method on a regular basis every two days. Twenty days after EB application, irrigation was terminated completely in half of the applications in order to form drought stress for 7 days. Among the studied traits, membrane damage index, leaf relative water content, amount of malondialdehyde, catalase enzyme activity, ascorbate peroxidase enzyme activity, and the contents of some mineral elements (K, Ca and Mg) were significantly different in drought stressed seedlings compared the control (regularly irrigated) seedlings. EB (Especially CA41/1) had generally positive effects on most studied traits, whereas drought stress had generally negative effects on the mentioned traits. There might be a high potential of EB fighting against drought stress in pepper; however, one keeps in mind that there is variation in the performance of EB; therefore, the best EB combinations have to be determined even for cultivars in each plant species in future studies. © 2021, Centenary University. All rights reserved.
Effects of Humic Acid, Whey and Arbuscular Mycorrhizal Fungi (Amf) Applications on Seedling Growth and Fusarium Wilt in Zucchini (Cucurbita Pepo L.)
(Pakistan Agricultural Scientists Forum, 2013) Sensoy, S.; Ocak, E.; Demir, S.; Tufenkci, S.
The effects of Arbuscular Mycorrhizal Fungi (AMF, Gigaspora margarita), whey (50 ml kg(-1)), and Humic acid (HA, 500 mg kg(-1)) applications on seedling growth and Fusarium wilt (FON, Fusarium oxysporum sp. niveum) in hybrid zucchini (Cucurbita pepo L.) cultivar Focus F-1 were investigated. The study was consisted of total 16 treatments including different combinations of FON, AMF, humic acid and whey. There were significant effects of the treatments on seedling growth and some nutrient contents. The treatment which had the highest suppression on Fusarium wilt was the single AMF treatment followed by triple application of AMF, HA, and whey.
Effects of Two Different Amf Species on Growth and Nutrient Content of Pepper Seedlings Grown Under Moderate Salt Stress
(Academic Journals, 2008) Turkmen, O.; Sensoy, S.; Demir, S.; Erdinc, C.
This study was carried out to determine the effects of different Arbuscular Mycorrhizal Fungi (AMF) species on the growth and nutrient contents of pepper seedlings (cv. Demre) grown under moderate salt stress. Two different mychorrhizas (Glomus intraradices and Gigaspora margarita) were tested on a growing media containing moderate salt stress (75 ppm NaCl). The study was replicated four times with 8 plants in each replicate. At the end of the study, some nutrients such as P, K, Ca, and Na and plant growth parameters such as shoot height, stem diameter, root length, and dry and fresh weights of shoots and roots were investigated. Saline condition had negative effects on the seedlings. Both AMF species had positive effects on salt tolerance based on the plant growth parameters and nutrient contents. G. intraradices caused better response in seedling development compared to G. margarita, though insignificantly.
Efficacy of Issr and Srap Techniques for Molecular Characterization of Some Cucurbita Genotypes Including Naked (hull-Less) Seed Pumpkin
(Pakistan Agricultural Scientists Forum, 2012) Inan, N.; Yildiz, M.; Sensoy, S.; Kafkas, S.; Abak, K.
This study was carried out to determine the efficacy of inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) techniques for molecular characterization some Cucurbita genotypes including naked (hull-less) seed pumpkin. Plant material consisted of sixteen genotypes belonging to Cucurbita pepo L., and four genotypes belonging to Cucurbita moschata Duchesne, and four genotypes belonging to Cucurbita maxima Duchesne. A larger part of the Cucurbita pepo genotypes (7 Turkish and 4 foreign) were seed pumpkin and some of them were naked seed types used as appetizer and/or for oil production. Morphological characterization was also done according to UPOV criteria. The sixty bands were obtained by using 8 ISSR primers and all these bands were found polymorphic. In the SRAP study, 8 primer combinations were used, a total of 71 bands were scored and all these bands were polymorphic. In the ISSR analyses, the genetic similarity coefficients varied between 0.07 and 0.96, while in SRAP it was between 0.13 1.0. The correlation coefficient between ISSR and SRAP genetic similarity data was very high (r = 0.947). The genetic variation as measured by ISSR and SRAP markers revealed high diversity among Cucurbita genotypes (H = 0.30, I = 0.46 and 100 % polym.). The genetic diversity among Turkish naked seed pumpkin genotypes (H = 0.09, I = 0.14 and 26.7 % polym.) was only a little less than that of the foreign accessions (H = 0.12, I = 0.18 and 32.1 % polym.). Some differences were observed between molecular and morphological studies, and it was concluded that only morphological or molecular analyses but a combination of both approaches are often more reliable in genetic variability studies in Cucurbita genus.
Evolution of Common Beans Collected From Lake Van Basin for Their Resistance To the Common Bacterial Blight (Xanthomonas Axonopodis Pv. Phaseoli)
(Corvinus Univ Budapest, 2018) Erdinc, C.; Ekincialp, A.; Akkopru, A.; Yildiz, M.; Sensoy, S.
The present study determined the reactions of common bean landraces grown in Lake Van Basin of Turkey against common bacterial blight disease (CBB) caused by Xanthomonas axonopodis pv. phaseoli (Xap). For this purpose 83 bean landraces collected from the basin and two resistant (HR-45, HR-67) and one susceptible (Dresden) lines were evaluated for their reaction to Xap. The experiments were conducted in randomized experimental design with three replications in a growth chamber having 23 +/- 2 degrees C temperature and 16 h light-8 h dark period. Bean seeds were sown in the pots having 2: 1 mixture of peat: perlite and Xap inoculated by spraying when the seedlings reached two trifoliate leaves stage. Disease severity was assessed three weeks after Xph inoculation using 1-5 scale. In light of the findings, it was detected that there was a variation with regard to tolerance to the disease among common bean landraces. While fourteen landraces were assigned as resistant against CBB, 49 landraces were found to be moderately susceptible to CBB. The present study demonstrated the existence of resistance sources against CBB within Lake Van Basin bean landraces that could potentially be used for breeding resistant cultivars.
Genetic Diversity in Some Turkish Pepper (Capsicum Annuum L.) Genotypes Revealed by Aflp Analyses
(Academic Journals, 2009) Aktas, H.; Abak, K.; Sensoy, S.
The genetic relationships among 14 Turkish pepper (Capsicum annuum L) genotypes, 6 of them inbred lines, were determined by comparing their molecular traits. The taxonomic relationships and genetic variation among these genotypes were investigated with those of 5 foreign pepper genotypes. Fifty-six (26%) polymorphic AFLP markers out of total 215 DNA fragments from 4 primer pairs were used to define the genetic similarity among the pepper genotypes by dendrograms or two and three dimensional scaling. Two genotype-specific markers for the genotype PM-702 were among the polymorphic ones. The inbred lines of Alata Agricultural Research Institution were partitioned to similar clusters and constituted extremely low genetic variation. On the other hand, other local Turkish genotypes had comparatively higher genetic diversity.
Genetic Relationships Among Various Sihke Melon Landraces
(Cukurova Univ, Fac Agriculture, 2012) Sensoy, S.; Sahin, U.
Hplc Analysis of Blackberry Fruits for Organic Acid and Sugar Contents
(int Soc Horticultural Science, 2015) Sensoy, I. Gazioglu; Gundogdu, M.; Sensoy, S.; Celik, F.; Dogan, A.
Blackberry has an important potential in fruit species because it is commonly used in both the fresh and processing market. In the present study, the organic acid (citric acid, malic acid, succinic acid, and fumaric acid) and sugar (fructose, glucose, and sucrose) contents of the blackberry genotypes grown in Van province of Turkey were determined. Organic acid and sugars are among the criteria in fruit maturation as well as they are effective in several physiological events. The citric acid was the predominant organic acid in the studied blackberry genotypes and ranged from 5.690 to 12.019 g/kg. The citric acid was followed by malic acid which varied from 1.412 to 6.346 g/kg. While the genotype 65ED02 had the highest total organic acid content (20.827 g/kg), the genotype 65ER01 had the lowest total organic acid content (8.469 g/kg). Fructose was the predominant sugar in the studied blackberry genotypes and ranged from 11.272 to 25.241 g/kg. While the genotype 65ED01 had the highest total sugar content 44.399 g/kg), the genotype 65GV01 had the lowest total sugar content (22.117 g/kg).
Imaging Techniques of Tomatoes (Solanum Lycopersicum L.) Grown With Different Organic and Conventional Fertilizer Applications
(Centenary University, 2023) Kipcak Bitik, S.; Sensoy, S.
In this study, which was carried out in field conditions in Van in 2019 and 2020, different image generation methods (biocrystallization method, and circular and rising picture chromatography methods) were used to determine the difference between various organic and conventional fertilizer applications. 3% frozen tomatoes and 16% CuCl2.2H2O were applied in the biocrystallization method, 80% frozen tomatoes and 1% silver nitrate solution were used in the circular chromatography method, and 100% frozen tomatoes and 0.5% silver nitrate and 0.5% iron sulfate solution were employed in the rising picture method. In light of the visual findings obtained at the end of the study, it has been determined that there are some differences between organic and conventional fertilizer applications. As the alteration between organic and conventional products; the center number difference in the copper crystallization method; the smoothness of the rings formed in the circular chromatogram and the vividness of the colors; in the rising painting method, it clearly reveals the transitions between colors and the difference in light and dark tones that occur in colors. © 2023, Centenary University. All rights reserved.
Iron (Fe) Deficiency Tolerance in Tomato Recombinant Inbred Lines Obtained From Tolerant and Sensitive Genotypes
(International Society for Horticultural Science, 2011) Bayram, M.; Sensoy, S.; Dasgan, H.Y.
The first aim of this study was to determine the iron (Fe) deficiency tolerance levels of the reciprocal recombinant inbred lines (RILs) in tomato. The RILs (F6 generation) have been created using tolerant and sensitive inbred parents for long time studies since 1994. The second aim was to obtain data which can be used to map the quantitative trait loci (QTL) for iron (Fe) deficiency tolerance in tomato. In this study, a total of 224 genotypes have been used. 107 genotypes contained the cytoplasm of the tolerant parent - 'Roza' - and 117 genotypes contained the cytoplasm of the sensitive parent-227/1. The plants were grown in a growth chamber in hydroponic medium. 35-day-old plants were subjected to additional 10 mM NaHCO3 to induce Fe-deficiency stress by stabilization of pH to 7.8-8.2. At least 10 plants from each F6 family were evaluated in each of five replications. After 10 days under Fe stress condition, plants were monitored for chlorosis. In order to quantitatively measure chlorophyll status, a Minolta chlorophyll meter (SPAD-502) was used to measure differential tones of green colour of young leaves. The plants were classified on the basis of SPAD and total Fe concentrations. The measured parameters of the RILs indicated the normal distributions. There were significant differences among the RILs for active, total irons (P≤0.05) and chlorophyll (P≤0.001) concentrations. It was concluded that Fe deficiency tolerance in tomato is inherited quantitatively and controlled by multi genes. The findings of this study showed there was a lack of strong definitive data supporting the possible cytoplasmic interactions in the inheritance of this trait.
Phenotypic and Molecular Genetic Diversityamong Some Turkish Bean Genotypes
(Pakistan Agricultural Scientists Forum, 2017) Erdinc, C.; Turkmen, O.; Dasgan, H. Y.; Sensoy, S.
The genetic relationships among 96common Turkish bean (Phaseolus vulgaris L.) genotypes were investigated based on72 phenotypic characters and molecular ISSR and RAPD markers. Main seed color and predominant secondary seed color were determined as the primary characteristics to distinguish the bean accessions. The mentioned traits, among 72 individual phenotypic traits, explained 58.46 % of phenotypic variation in the first three axes of the principal component analysis (PCA) beside flower duration, plant height, and a number of nodes on the stem. The 21 ISSR primers and 8 RAPD primers having clear and readable band data were also employed, 358 and 116 polymorphic bands were obtained from them, respectively. Polymorphism information content value for ISSR varied between 0.15 and 0.50, while that for RAPD was from 0.31 to 0.48. Among the examined genotypes, molecular genetic relationship determined based on dendrogram obtained by Jaccard distance matrix. Based on the results, the 52% and 48% of the bean genotypes were categorized as Mesoamerican and Andean originated genotypes. In addition, genetic variation values were determined by using Nei and Shannon coefficients and they were highly variable among bean genotypes.
Response of Four Zucchini (Cucurbita Pepo L.) Hybrids To Different Arbuscular Mycorrhizal Fungi
(Pakistan Agricultural Scientists Forum, 2011) Sensoy, S.; Demir, S.; Tufenkci, S.; Erdinc, C.; Demirer, E.; Unsal, H.; Ekincialp, A.
Four zucchini hybrids (Focus F-1, Comet F-1, Natali F-1, and Ezra F-1) inoculated by three different arbuscular mycorrhizal fungi (AMF) [Glomus intraradices (Gi), Glomus etinucatum (Ge), and Gigaspora margarita (Gm)] in a growth chamber experiment were evaluated for seedling traits, nutrient uptake, colonization, and relative mycorrhizal dependency (RMD). Relative mycorrhizal dependency ranged widely among four zucchini hybrids. Gm inoculations had higher positive RMDs, while Gi inoculations had lower negative RMDs. Gm-inoculated Focus F-1 had the highest RMD (30.22%). Arbuscular mycorrhizal fungi inoculated seedlings had wider cotyledons and stems. There were also significant effects of AMF inoculation on the most of nutrients.
Response of Turkish Melon Genotypes To Fusarium Oxysporum F. Sp Melonis Race 1 Determined by Inoculation Tests and Rapd Markers
(Eugen Ulmer Gmbh Co, 2007) Sensoy, S.; Demir, S.; Buyukalaca, S.; Abak, K.
The response to Fusarium oxysporum f. sp. melonis (F.o.m.) race 1 of 79 Cucumis melo L. genotypes - 56 accessions collected from different parts of Turkey, 5 local cultivars and 18 foreign genotypes - was determined by using pathogenicity tests and RAPD markers (E07 and G17). For pathogenicity tests, at least 15 individuals of each melon genotype were artificially inoculated by either conidia suspension culture or sand culture of F.o.m. race 1. Melon seedlings were evaluated on a 0-3 scale for the presence of vascular browning two weeks after inoculation with conidia suspension culture resp. four weeks after inoculation with sand culture. The disease incidence on the 79 melon genotypes ranged from 0 to 100%. Seven melon genotypes were resistant (CU129, CU258, T8, Y9, Y10, Y15, and Y63), three melon genotypes (CU280, CU309, and T1) were heterogeneous, and all the others were susceptible to F.o.m. race 1 based on disease incidence and vascular browning. The success of the E07 marker in detecting susceptible genotypes was higher than that of G17; the mistmatch ratios of E07 and G17 markers were 5.06 and 58.23%, respectively.
Sirmo (Album Spp.), Wild Herb Species Used in Herby Cheese
(int Soc Horticultural Science, 2016) Guldigen, O.; Sensoy, S.
There are several herbs used in herby cheese production in Eastern and Southeastern Anatolia regions in Turkey. Sirmo (Allium spp.), known as wild garlic, is the most widely known herb among them. Sirmo is collected before flowering in the spring of the surrounding country land and is added into the herby cheese whilst fresh or pickled with other herbs (mostly belongs to Apiaceae). The above-ground parts of Sirmo plants other than bulb and flowers are collected while budding, sliced into 1-3 cm pieces, and added into herby cheese. Some Sirmo species are used in the region as vegetables and spices. There are at least eight Sirmo species (Allium atroviolaceum, A. aucheri, A. cardiostemon, A. fuscoviolaceum, A. paniculatum, A. schoenoprasum L., A. scorodoprasum L. subsp. rotundum, and A. vineale L.) widely used in herby cheese. Sirmo gives a special flavor to herby cheese and it has the highest vitamin C content among other herbs. Sirmo has one of the highest total antioxidant capacities in trolox equivalents of herbs used in herby cheese. Conditions might activate some pathogens in traditionally produced herby cheese. However, studies indicate that Sirmo species have inhibitory effect on these pathogens. Sirmo positively affects some beneficial microbial activity in herby cheese and could increase its nutritional content. The majority of Sirmo species commonly used in herby cheese in the region has not yet been cultivated and there is lack of information about their cultivation possibilities. In addition, there is no established standard for the use of Sirmo as well as other herbs in cheese production. This review gives information about these wild Allium spp. used in herby cheese and discusses their future.