Phenotypic and Molecular Genetic Diversityamong Some Turkish Bean Genotypes

Abstract

The genetic relationships among 96common Turkish bean (Phaseolus vulgaris L.) genotypes were investigated based on72 phenotypic characters and molecular ISSR and RAPD markers. Main seed color and predominant secondary seed color were determined as the primary characteristics to distinguish the bean accessions. The mentioned traits, among 72 individual phenotypic traits, explained 58.46 % of phenotypic variation in the first three axes of the principal component analysis (PCA) beside flower duration, plant height, and a number of nodes on the stem. The 21 ISSR primers and 8 RAPD primers having clear and readable band data were also employed, 358 and 116 polymorphic bands were obtained from them, respectively. Polymorphism information content value for ISSR varied between 0.15 and 0.50, while that for RAPD was from 0.31 to 0.48. Among the examined genotypes, molecular genetic relationship determined based on dendrogram obtained by Jaccard distance matrix. Based on the results, the 52% and 48% of the bean genotypes were categorized as Mesoamerican and Andean originated genotypes. In addition, genetic variation values were determined by using Nei and Shannon coefficients and they were highly variable among bean genotypes.