Browsing by Author "Sensoy, Suat"

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The Application of Nanoparticles on the Physiological, Morphological, Enzyme Activities, and Nutrient Uptake of Lettuce Under Different Irrigation Regimes
(Springer, 2025) Kilic, Hilal Karacan; Cakmakci, Talip; Sensoy, Suat
This study aimed to determine the effects of selected plant growth-promoting silver nanoparticles (AgNPs) on certain physiological traits, plant growth, enzyme activity, and plant nutrient content of lettuce grown under different irrigation regimes. In this research, four irrigation regimes were tested as experiments in a randomized complete block design layout: I100 (full irrigation), I80 (20% deficit), I60 (40% deficit), and I40 (60% deficit). Additionally, four nanoparticle doses (AgNP0, 0 ppm; AgNP20, 20 ppm; AgNP40, 40 ppm; and AgNP80, 80 ppm) were applied to the crop. The results demonstrated that the head diameter of lettuce decreased by 28% and 30% under the I60 and I40 water stress conditions, respectively. However, AgNPs enhanced the head diameter of lettuce by 10.9% compared to the control (non-application of AgNPs). The application of AgNPs increased the nutrient content of lettuce, including potassium (K), sodium (Na), iron (Fe), copper (Cu), and manganese (Mn), by 38%, 19%, 23%, 367%, and 20%, respectively. Plant height was 98.2% higher in the full irrigation treatment (I100) than in the 40% irrigation treatment (I40). Dry matter content was 10.2% higher in the AgNP80 treatment compared to the AgNP0 treatment. In addition, the highest catalase (CAT) enzyme content was obtained in the AgNP40 treatment. The present research suggests that applying AgNPs represents a sustainable and eco-friendly strategy for improving lettuce's plant growth and nutrient uptake under water stress conditions. Therefore, AgNPs can be used in drought stress tolerance studies.
Arbuscular Mycorrhizal Fungi Affect Seedling Growth of Melon Hybrid Cultivars
(Friends Science Publ, 2013) Sensoy, Suat; Bicer, Seymus; Unsal, Husameddin
Hybrid melon cultivars (Super Magnum F-1, Extra Early Galia F-1, Rambo F-1 and Sempati F-1) were inoculated with 3 different AMF (Arbuscular Mycorrhizal Fungi) [Gigaspora margarita (Gm), Glomus intraradices (Gi), and G. etunicatum (Ge)] in order to examine the effects of AMF on seedling growth in melon. The seedling traits, nutrient uptake, colonization, and relative mycorrhizal dependency (RMD) were assessed in the experiment. Mycorrhizal colonization of melon cultivars ranged from 38.9 to 54.9%. The nitrogen, phosphorous, potassium, magnesium, and manganese contents were affected by melon cultivars, AMF, or their combinations. Relative mycorrhizal dependency also varied widely; only half of the melon cultivar-mycorrhizae combinations showed positive mycorrhizal dependencies. The Gi inoculations had higher positive RMDs, while the Gm inoculations had lower negative RMDs. (c) 2013 Friends Science Publishers
Bioactive Components and Antioxidant Capacities of Different Miniature Tomato Cultivars Grown by Altered Fertilizer Applications
(Springer, 2018) Erdinc, Ceknas; Ekincialp, Aytekin; Gundogdu, Muttalip; Eser, Fuat; Sensoy, Suat
This study investigated the organic acid and phenolic compound levels, total phenolic (TP) and antioxidant capacity (TEAC) of three miniature tomato cultivars grown on a farmer's field with three different fertilizer applications. Analysis of phenolic compound (protocatechuic, vanillic, gallic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid, rutin, quercetin, catechin and phloridzine) organic acid (citric, tartaric, malic, succinic and fumaric acid), TP and TEAC levels in fruit samples showed statistically significant (p < 0.05) differences between tomato cultivars and fertilizer applications. Rutin and chlorogenic acid were the predominant phenolic compounds found in all three cultivars. The highest rutin value (50.48 mg kg(-1) FW) was found in the Black Zebra fertilized with DAP plus organic and biochemical fertilizers and the lowest (20.52 mg kg(-1) FW) in the Black Zebra fertilized with DAP only. The highest chlorogenic acid value (63.31 mg kg(-1) FW) was found in the sweet pea currant fertilized with DAP only and the lowest (21.06 mg kg(-1) FW) in the Black Zebra fertilized with DAP plus chemical fertilizer. Citric acid content was the dominant organic acid in all three cultivars, with the highest citric acid value (6439.50 mg kg(-1) FW) found in the Sweet Pea Currant cherry tomato fertilized with DAP plus organic and biochemical fertilizers and the lowest (2435.20 mg kg(-1) FW) in the Window Box Yellow fertilized with DAP plus chemical fertilizer. Total phenolic and antioxidant levels in the window box yellow were significantly lower as compared to the Black Zebra and sweet pea currant varieties for all three fertilizer applications.
Bioactive Constituents of Allium Vineale L. Accessions From Eastern Turkey
(Elsevier, 2022) Cakmakci, Ozlem; Sensoy, Suat; Alan, Ali R.
Edible parts of Alliums are rich sources of health promoting bioactive compounds such as polyphenols, flavonoids, and antioxidants. These compounds are known to promote human health by reducing the risk of various cancer, cardiovascular and brain and diabetes diseases. Allium vineale is an edible wild Allium species collected from nature and used in herbed cheese production. Quality of herbed cheese is associated with A. vineale leaves, the main ingredient used as fresh or dried in the process of cheese production. The aim of this study was to evaluate bioactive constituents in above ground organs of 10 accessions from an A. vineale germplasm collected from Van province of Turkey. Each accession was represented with 10 plants in the analyses for bioactive compounds. It was found that A. vineale accessions were significantly different from each other for their bioactive content levels. Statistically significant differences were found among the accessions for total phenolic content and antioxidant activities. On average, A. vineale samples contained 60.11 g GAE kg(-1) total phenolic compound and 98.84 mmol TR kg(-1) total antioxidant capacity. The main phenolic compound detected in A. vineale was protocatechuic acid (similar to 221 g kg(-1)). The findings obtained from this study provide an opportunity for selecting genetically superior accessions with high bioactive compound production as starting materials in the improvement of new A. vineale cultivars suited for controlled cultivation.
Biochemical and Morphological Characteristics of Some Macrofungi Grown Naturally
(Mdpi, 2021) Selem, Ezelhan; Alp, Yekbun; Sensoy, Suat; Uzun, Yusuf; Cavusoglu, Seyda; Karatas, Neva; Szopa, Agnieszka
Recently, the production of macro-fungi (mushrooms) has steadily increased, and so has their economic value, in global terms. The use of functional foods, dietary supplements, and traditional medicines derived from macro-fungi is increasing as they have numerous health benefits as well as abundant nutrients. This study aimed to determine some biochemical contents (pH, soluble solid contents (SSC), total antioxidant capacity (TAC) and total phenolic contents (TPC)) of eight edible macro-fungi species growing naturally (in the wild) in Turkey. The samples were collected in the Van Yuzuncu Yil University (VAN YYU) campus area in the months of April-May 2018, in different locations, and brought to the laboratory, and the necessary mycological techniques were applied for their identification. Location, habitats, collection dates and some morphological measurements were determined for all identified species. Biochemical parameters of the macro-fungi species were analyzed separately both in cap and stem. The color values (L, a, b, Chroma and hue) were separately evaluated on cap surface, cap basement and stem. Results showed that there were significant differences for most of the biochemical parameters in different organs between and within species. The pH, SSC, TAC and TPC values varied from 6.62 to 8.75, 2.25 to 5.80 & DEG; brix, 15.72 to 57.67 TE mg(-1) and 13.85 to 60.16 gallic acid equivalent (GAE) fresh weight basis. As a result of the study, it was concluded that the parameters such as total antioxidant capacity, total phenolic content and soluble content in Morchella esculenta, Helvella leucopus, Agaricus bitorquis and Suillus collinitus were higher than for the other species and clearly implied that they may be further exploited as functional ingredients in the composition of innovative food products.
Comparison of Some Local Melon Genotypes Selected From Lake Van Basin With Some Commercial Melon Cultivars for Some Yield and Quality Related Traits Observed in Field and High Tunnel Conditions
(Academic Journals, 2008) Erdinc, Ceknas; Turkmen, Onder; Sensoy, Suat
This study aimed to compare some local melon (Cucumis melo L.) genotypes selected from the Lake Van Basin (65 ER 02, 65 ER 04, and 13 TAT 05) with some commercial melon cultivars (Ananas, Makdimon F(1), and Rambo F(1)) for some yield and quality related traits observed in field and high tunnel conditions for two years. At the end of the study, it was determined that Makdimon F(1) produced the highest early and total yields (14.76 and 30.51 t ha(-1), respectively). While the locally selected melon genotype 65 ER 04 followed Makdimon F(1) for the early yield (11.43 t ha(-1)), the locally selected genotype 65 ER 02 followed Makdimon F1 for the total yield (22.49 t ha(-1)). The cultivars Rambo F(1) and Makdimon F(1) had higher soluble solid contents than the others.
Detection of Active Substances in Cynara Syriaca B. and Cynara Scolymus L. Type Artichokes Growing Naturally in Siirt Region
(Parlar Scientific Publications (p S P), 2020) Aktar, Yunus; Karipcin, M. Zeki; Sensoy, Suat
This project was carried out to determine the active ingredient contents in artichoke plant, an ancient and interesting vegetable which gave its name to festivals. Artichoke; which is a vegetable that is very important for human beings in terms of health, has also taken place in mythology. The study was carried out in Siirt University, Faculty of Agriculture; Department of Horticulture. The experiment was conducted in Turkey; in the city of Siirt and the province of Kezer; one of the few places in which Cynara syriaca species grow. Different branching types (1 branch, 7 branch; 14 branch, 21 branch and 28 branch) of Cynara syriaca species in the region were used in the experiment. In addition, other varieties of Cynara scolynnts species (Saktz and Bayrampaa) which have been cultivated and taken into our food culture were also used. Cultivated species were grown in controlled environments and samples were obtained from these controlled areas, The wild type (Cynara syriaca) samples were obtained from different types of plants grown in the natural environment. Determination of active ingredient contents in leaf samples belonging to different artichoke varieties and types were performed. With the determination of active substance: active substance/branch number, active substance/head weight and active substance/head number measurements were also performed. Morphological properties of both species were also investigated. Due to the fact that wild artichokes are prickly and they grow in natural environment, it is expected that their active ingredient contents will be higher. However. at the end of the study, contrary to expectations, it was found that the cultivated artichoke varieties (Bayrampaa and Sakiz) had a richer content in terms of active substance than Cynara syriaca species with different branching characteristics. Cynara syriaca genotypes were found to have higher values in terms of active substance/branch number, active substance/head weight and active substance/head number. Whether or not a plant can he used as a drug is decided according to the active ingredient content of the same plant.
Determination of Plant-Pan Coefficients for Field-Grown Eggplant (Solanum Melongena L.) Using Class a Pan Evaporation Values
(Elsevier, 2006) Ertek, Ahmet; Sensoy, Suat; Kucukyumuk, Cenk; Gedik, Ibrahim
This study was conducted to determine the most suitable plant-pan coefficient for eggplant, using pan evaporation values, in field conditions. Application of irrigation water was based on cumulative class A pan evaporation within the irrigation intervals. Irrigation treatments consisted of one irrigation interval (11: 7 day) and five plant-pan coefficients (K(cp)1: based on percent crop canopy closure; K(cp)2: 0.50; K(cp)3: 0.70; K(cp)4: 0.90; K(cp)5: 1.10). Average irrigation values for each treatment varied from 372 to 689 mm, average evapotranspiration values ranged from 420 to 689 mm and eggplant fruit yield ranged from 10.11 to 21.14 tonnes ha(-1). The highest total and early fruit yield was obtained from the K(cp)4 and K(cp)3 treatments, respectively. Seasonal yield response factor (Ky) was 0.60. E-t/E-pan ratios for each treatment varied from 0.18 to 1.32. In addition, irrigation levels substantially affected yield (P < 0.001) and fruit number (P < 0.01). A significant correlation between irrigation water volume and plant vegetative growth traits, and between plant water consumption and fruit yield, were found. Thus, in irrigation programming, the Kcp4 plant-pan coefficient is recommended for field-grown eggplant. (c) 2006 Elsevier B.V. All rights reserved.
Effect of Pre-Harvest Organic Cytokinin Application on the Post-Harvest Physiology of Pepper (Capsicum Annuum L.)
(Mdpi, 2021) Cavusoglu, Seyda; Sensoy, Suat; Karatas, Arzu; Tekin, Onur; Islek, Firat; Yilmaz, Nurettin; Mlcek, Jiri
Peppers are cultivated in almost all provinces in Turkey, making up approximately 7% of the world's production. In this study, the effects of pre-harvest cytokinin application on the postharvest physiology of pepper (Capsicum annuum L.) fruits (cv. Akra and Melek F1) grown in ecological conditions in Turkey (Van, eastern Turkey) were investigated. During the growing period, organic cytokinin(nytrozine) at two concentrations (50 and 100 ppm doses) was applied by spraying. Peppers were harvested in green maturity and then stored at 12 degrees C for 28 days. Changes in color, respiration rate, exogenous ethylene amount, antioxidative enzyme analysis (SOD, CAT and APX), lipid peroxidation, total phenolic content (TP) and antioxidant activity (AA) were determined just after the harvest and then at the 7th, 14th, 24th and 28th days. L* values for both varieties and the control sample had fluctuations during storage, and they slightly decreased after 28 days, except for the end value of cv. Melek F1 with a 50 ppm addition. In terms of respiration rate values, the difference between applications was found to be statistically significant, except for the 14th and 24th days of storage, for cv. Akra F1; and the 0th and 28th days for cv. Melek F1, respectively. It was observed that cytokinin can delay aging in peppers of both cultivars stored for 28 days. As a result, as the used cytokinin has an organic origin, is not synthetic and does not have a negative effect on human health, it can be used safely and without a significant loss of quality characteristics for peppers. These applications have positive effects on in-package gas composition, antioxidative enzymes, lipid peroxidation, total phenolics and total antioxidant activity.
Effect of Vermicompost Application on Some Plant Characteristics in Lettuce (Lactuca Sativa L.)
(Parlar Scientific Publications (p S P), 2018) Kabay, Turgay; Alp, Yekbun; Sensoy, Suat
Vermicompost, an organic material, has a positive effect on plant growth and can be applied to all areas where organic farming is conducted. This study was carried out as a pot experiment in greenhouse conditions in order to determine the effects of vermicompost application on plant growth in lettuce cultivation. Lettuce seeds were sown in the vials containing 2: 1 mixture of peat and perlite. After the cotyledon leaves emerged, the seedlings were transferred into the 2 liters pots. Then, the control plants grown in peat (1/2 volume) + perlite (1/2 volume) media were irrigated with Hoagland nutrient solution until the end of the experiment, whereas the vermicompost applied plants grown in peat (1/3 volume)+perlite (1/3 volume) + vermicompost (1/3 volume) media were irrigated with only tap water. Twenty-seven days from the planting, the differences in seedling fresh and dry weights, shoot weights, stem diameters, root fresh and dry weights, leaf numbers, leaf temperatures, leaf areas, chlorophyll contents, total phenolic contents, total antioxidant values and antioxidative enzymes were determined. Considering these parameters, it can be said that vermicompost application has positive effects on lettuce growth.
Effects of Arbuscular Mycorrhizal Fungi in Melon (Cucumis Melo L.) Seedling Under Deficit Irrigation
(Parlar Scientific Publications (p S P), 2017) Cakmakci, Ozlem; Cakmakci, Talip; Durak, Emre Demirer; Demir, Semra; Sensoy, Suat
Melon (Cucumis melo L.) is an important vegetable and is growth in Eastern Anatolia region of Turkey since the early times. Drought is effective abiotic stress factors for plants. As is known that arbuscular mycorrhizal fungi (AMF) have positive effects on plant growth and arbuscular mycorrhizal symbiosis can play a prominent role in tolerance to abiotic stress factors. Plant material used in this study is cv. Napolyon F-i cultivated commonly in the Lake Van Basin. The effects of two different mycorrhiza applications (Glomus intraradices and Glomus spp.) on some growth parameters in melon seedlings under water deficit were investigated. There were four different water deficit applications (control, 20 %, 40 % and 60 % water deficit). At the end, it was found that mycorrhiza improved the physiological and photosynthetic parameters of inoculated plants compared with non-AMF plants in water deficit conditions. Moreover, AM Fungi increased the chlorophyll content of melon seedlings. Results indicated that AMF can ameliorate the tolerance to deficit irrigation in melon seedlings.
The Effects of Arbuscular Mycorrhizal Fungi on the Seedling Growth of Four Hybrid Cucumber (Cucumis Sativus L.) Cultivars
(Tubitak Scientific & Technological Research Council Turkey, 2012) Tufenkci, Sefik; Demir, Semra; Sensoy, Suat; Unsal, Husameddin; Demirer, Emre; Erdinc, Ceknas; Ekincialp, Aytekin
The effectiveness of different arbuscular mycorrhizal fungi (AMF) on different hybrid cucumber cultivars has not been well documented, even under normal seedling conditions. The present study therefore aimed to evaluate colonization, nutrient uptake, dependency, and other seedling traits of 4 cucumber hybrids (Ceren F-1, Beta F-1, Silyon F-1, and Maraton F-1) inoculated by 3 different AMF [Glomus intraradices (Gi), Glomus etunicatum (Ge) and Gigaspora margarita (Gm)]. Traits were evaluated in a growth chamber experiment consisting of a 4 x 4 factorial design (4 cucumber hybrids, 3 AMF plus 1 control) with 3 random replications. AMF-inoculated cucumber seedlings had shorter hypocotyledons and wider and longer cotyledons than non-inoculated seedlings. Gm-inoculated seedlings had the narrowest stem diameter and lowest leaf number. AMF-inoculated seedlings had shorter shoots and longer roots than noninoculated ones. There was significant mycorrhizal effect on the iron (Fe) content of shoots and the mycorrhizal colonization rate in roots. Relative mycorrhizal dependency (RMD) varied widely among the hybrid cucumber cultivars tested. It is thought that the determination of high-RMD cultivars could lead to improvements in cucumber seedling production in the future.
Effects of Arbuscular Mycorrhizal Fungus, Humic Acid, and Whey on Wilt Disease Caused by Verticillium Dahliae Kleb. in Three Solanaceous Crops
(Tubitak Scientific & Technological Research Council Turkey, 2015) Demir, Semra; Sensoy, Suat; Ocak, Elvan; Tufenkci, Sefik; Durak, Emre Demirer; Erdinc, Ceknas; Unsal, Husamettin
This study examined the effects of arbuscular mycorrhizal fungi (AMF), humic acid (HA), and whey (W) application on wilt disease caused by Verticillium dahliae Kleb. in tomato, pepper, and eggplant. Single, dual, and triple applications of AMF (2.5 g inocula of either Glomus mosseae or G. intraradices), HA (500 ppm), and W (50 mL kg(-1)) were found to improve the morphological growth and nutritional status of all three host species. Moreover, dual and triple applications reduced the severity of wilt disease caused by V. dahliae by between 40% and 70.5%. Triple application of AMF, HA, and W decreased the number of V. dahliae microsclerotia by 50%. Furthermore, W and HA application promoted AMF growth, with HA application resulting in significantly higher levels of AMF colonization and spore density when compared to untreated controls.
Effects of Different Arbuscular Mycorrhizal Fungi (Amf) Species on Some Bean (Phaseolus Vulgaris L.) Cultivars Grown in Salty Conditions
(Academic Journals, 2010) Ciftci, Vahdettin; Turkmen, Onder; Erdinc, Ceknas; Sensoy, Suat
This study was carried out to determine the effects of three different Arbuscular Mycorrhizal Fungi (AMF) species (Glomus mosseae, Glomus intraradices and Glomus fasciculatum) on the growth and nutrient contents of four bean cultivars (Onceler, Seker, Terzibaba and Sehirali) grown under salt stress. The constant amount of NaCl (50 ppm) was added the autoclaved growth medium containing 1:1:1: ratios of soil, sand, and manure. The five gram (25 spores g(-1)) of inoculum was placed in the seedling growth medium before the seeds were sown. At the end of the study, some nutrients such as N, P, K, Ca, Mg, Fe, Cu, Mn, and Zn and plant growth parameters such as shoot height, stem diameter, root length, leaf number, leaf area, and dry and fresh weights of shoots and roots were investigated. Moreover, the plant colonization rates of AMF species were determined. The AMF species had positive effects on the plant growth and nutrient intake. Among the bean cultivars, Onceler and Terzibaba, and among the AMF species, G. mosseae had the best results for plant growth.
Evaluation of Genetic Diversity in Turkish Melons (Cucumis Melo L.) Based on Phenotypic Characters and Rapd Markers
(Springer, 2007) Sensoy, Suat; Buyukalaca, Saadet; Abak, Kazim
The genetic relationships among 56 melon (Cucumis melo L.) genotypes collected from various parts of Turkey were determined by comparing their phenotypic and molecular traits with those of 23 local and foreign melon genotypes to investigate the taxonomic relationships and genetic variation of Turkish melon germplasm. Sixty-one phenotypic characters and 109 polymorphic RAPD markers obtained from 33 primers were used to define the genetic similarity among the melon genotypes by dendrograms or two and three dimensional scaling. There were high correlations ( r >= 0.97) among the four resulting matrices used in molecular characterization. The correlations between phenotypic ( Euclidean) and molecular Euclidean, Jaccard, Simple matching, and Nei analyses were r = 0.41, r = -0.40, r = -0.43 and r = -0.40, respectively. Related genotypes or genotypes collected from similar regions were partitioned to similar clusters. Both analyses ( phenotypic and molecular) indicated that non-sweet melon types were dissimilar from sweet types and diversity of Turkish melon genotypes was higher than that of sweet foreign cultivars examined, but similar to that of the reference accessions employed. It was also observed that sweet Turkish melon genotypes belonging to groups inodorus and group cantalupensis were highly variable and could have intermated or have crossed with other non-sweet types.
The First Report About Genetic Diversity Analysis Among Endemic Wild Rhubarb (Rheum Ribes L.) Populations Through Ipbs Markers
(Tubitak Scientific & Technological Research Council Turkey, 2021) Erdinc, Ceknas; Ekincialp, Aytekin; Turan, Sibel; Kocak, Metin; Baloc, Faheem Shahzad; Sensoy, Suat
Approximately 30% of plant species of Turkey, which is among the richest countries in terms of biodiversity, has been endemic. Wild rhubarb (Rheum ribes L.) is a wild vegetable grows especially in the eastern region of Turkey and is an endemic species. In this study, genetic relationships among 80 wild rhubarb genotypes collected from some regions of Lake Van Basin, which are in the distribution area, were tried to be determined by iPBS marker system. At the same time, a commercial variety of R. rhabarbarum, which is a cultivated species, was used as control. PCR studies were conducted with 23 iPBS primers to determine genetic relationships, and a total of 340 scorable bands were obtained. 100% polymorphism rate was obtained from all primers studied. While the average PIC value was found to be 0.90, the highest value was found to be 0.97 from the primer # 2220. It was determined that the genotypes were divided into 3 basic groups in the dendogram created with UPGMA based on Jaccard similarity coefficient.
Fruit Quality Parameters and Molecular Analysis of Apple Germplasm Resources From Van Lake Basin, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2015) Kaya, Tuncay; Balta, Fikri; Sensoy, Suat
The objective of this study was to determine some fruit quality characters and genetic variability of native apple germplasm resources from Van Lake Basin, eastern Turkey. With respect to fruit quality characters, apple genotypes had a range of 46.00-94.99 mm for fruit diameter, 43.04-310.99 g for fruit weight, 0.71-1.13 for fruit shape index, 3.99-14.05 kg cm(-2) for fruit flesh firmness, 9.0%-14.4% for soluble solids content, and 0.15%-1.75% for titratable acidity. Five genotypes revealed no alternate bearing and nine genotypes showed partial alternate bearing. The genetic variability was characterized by random amplified polymorphic DNA (RAPD) for 35 native apple genotypes belonging to the basin and two standard apple cultivars. The average level of polymorphism across genotypes was 89.29% as revealed by RAPD. The highest genetic similarity was detected between the G10 and G11 (79.31%) genotypes and the similarity rate varied between 38% and 79% among genotypes. According to the dendrogram derived from RAPD data, the G3, G23, G28, and G24 genotypes formed a cluster. These results implied that the Van Lake Basin may be one of the native expansion areas of the genus Malus.
Genetic Characterization of Rheum Ribes (Wild Rhubarb) Genotypes in Lake Van Basin of Turkey Through Issr and Ssr Markers
(Friends Science Publishers, 2019) Ekincialp, Aytekin; Erdinc, Ceknas; Turan, Sibel; Cakmakci, Ozlem; Nadeem, Muhammad Azhar; Baloch, Faheem Shehzad; Sensoy, Suat
Rheum ribes L. (wild rhubarb) is one of the less known plants to the world and the only species from the Rheum genus present in Turkey. In this study, one R. rhabarbarum (as check genotype) and 80 R. ribes genotypes were collected from different geographical locations of Turkey for the investigation of diversity and genetic structure using ISSR (Inter Simple Sequence Repeat) and SSR (Simple Sequence Repeats) markers. SSR markers reflected higher (100%) polymorphism as compared to the ISSR marker. However, ISSR markers produced higher average Polymorphism Information Content (PIC) value (0.805) than the SSR markers (0.724). A Similar range of (PIC) values with ISSR markers was found greater (0.935-0.395) as compared to the range of SSR makers (0.88-0.47). Using Jaccard similarity index, genetic distance was measured for both markers and average genetic distance was found to be higher with ISSR markers as compared to the SSR markers. Neighbor-joining analysis clustered genotypes into 3 groups for both marker systems. During this study some distinct genotypes like R. rhabarbarum, YYUERC19, YYUERCO9 and YYUNIER65 were investigated that can be used as candidate parents for the development of R. ribes L. varieties. Structure analysis grouped the genotypes according to altitude by clustering genotypes having at more than 2000 m in one group and genotypes less than 2000 m altitude in another group. Genetic variations observed in this study can be applied to investigate various traits of interest for the R. ribes L. breeding. (C) 2019 Friends Science Publishers
Genetic Diversity Among Some Asparagus Species Using Rdna Its, Cpdna Trnl Intron Sequence and Screening for Antioxidant Activity
(Hard, 2019) Altintas, Serdar; Pakyurek, Mine; Sensoy, Suat; Erez, M. Emre; Inal, Behcet
Three species of genus Asparagus native to Lake Van Basin of Turkey were analyzed using internal transcribed spacer (ITS) and cpDNA trnL intron sequence. Furthermore, these species were screened for their antioxidant activity and total phenolic and flavonoid contents. Phylogenetic analysis based on ITS data revealed two clades: the first clade consisted of Asparagus persicus and Asparagus officinalis, and clade II consisted of Asparagus palaestinus. Similarly, phylogenetic analysis using trnL intron sequence revealed two clades. In term of total phenolic content and total flavonoid content, the highest value were measured for acetone extract of Asparagus persicus sample No. 2 and Asparagus persicus sample No. 6, respectively. The highest antioxidant activity using FRAP assay was found for Asparagus persicus sample No. 3. We concluded that these species could be useful for increasing genetic diversity among cultivated species and for breeding.
The Impact of High Temperatures on Some Plant Growth Parameters in Some Common Bean Genotypes
(Hard, 2022) Kabay, Turgay; Ekincialp, Aytekin; Sensoy, Suat
Common bean is one of the most cultivated vegetables globally and in Turkey. Especially in areas with a temperate climate, the losses in productivity and quality limit production due to high temperatures. Thus, the present study aimed to determine the bean genotypes tolerant to high temperature stress and to reduce the above-mentioned yield and quality losses to benefit the researchers and producers. In the present study, 46 bean genotypes and 2 bean cultivars, determined to be tolerant and sensitive to high temperatures in previous studies, were used. Variations in 0-5 visual scale value, plant fresh and dry weight, root fresh and dry weight, stem diameter, plant height, leaf number and area, relative leaf water content, membrane damage index and ion (K and Ca) content were determined in bean plants. Bean seeds were sown in 2-liter pots containing 2:1 peat + perlite mixture, 3 plants per pot based on the randomized blocks experimental design. From the seed sowing until the first real leaf appearance, the pots were irrigated with tap water and then, the pots were irrigated with Hoagland nutrient solution. The control plants were grown in a polyethylene greenhouse and the plants subjected to high temperature were grown in an extra polyethylene covered high tunnel in a polyethylene greenhouse to create high temperature stress. There was a daytime temperature difference of 6 degrees C (optimum: 32.17 degrees C for high temperature application; optimum: 26.17 degrees C for control application) between the control and high temperature applications. The analyzes conducted on the plants revealed that the genotypes V21, V28, V32, V82, T65, and V-a1 and cv. Yakutiye were tolerant to high temperature stress, while the genotypes V88, V89, T7, and T72 and cv. Zulbiye were the sensitive ones.