Browsing by Author "Stormo, Keith"

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The Effectiveness of Recombinant Ol Fusion Protein (ovalbumin-Lhrh in Suppressing Reproductive Functions When Injected in Single-Dose Vaccination Protocols With Different Adjuvants
(Elsevier Science Bv, 2013) Karakus, Ferda; Yilmaz, Ayhan; Hakan, Bunyamin; Stormo, Keith; Ulker, Hasan
The objective of this study was to evaluate the effectiveness of recombinant LHRH fusion protein, Ovalbumin-LHRH-7 (OL), using a single-dose vaccination protocol in combination with different adjuvants in suppressing reproductive functions in buck kids. For this purpose, either a mixture of free OL antigen and encapsulated OL antigen, or encapsulated OL antigen was used. Thirty-nine native buck kids at 12 weeks of age were divided into control (n = 7) and treatment groups (n = 8 bucks/group). The four treatment groups were formed according to the different vaccine formulations: Group CpG received 0.5 mg free OL protein together with 1.0 mg of encapsulated protein with CpG adjuvant. Group mFCA received 0.5 mg free OL protein together with 1.0 mg of encapsulated protein with modified Freund's complete adjuvant. Group IS received 1.5 mg encapsulated OL protein with a mix of inulin and saponin adjuvants. Group ISmFCA received 1.5 mg encapsulated OL protein with a mix of inulin, saponin and modified Freund's complete adjuvants. Scrotal circumference in CpG and mFCA groups were significantly smaller than that of Control, IS and ISmFCA groups (P < 0.05). Numbers and percentage of bucks having spermatozoa in their ejaculate were significantly lower in CpG and mFCA groups (P < 0.05). OL immunization completely suppressed sperm production, except one buck, in CpG and mFCA groups (P < 0.05). These results imply that it is possible to use OL protein in a single injection protocol for the purpose of immunocastration. Further investigation with a larger number of animals should be carried out to determine the longevity of response to a single injection. (C) 2013 Elsevier B.V. All rights reserved.
Evaluation of One-Shot Vaccination Protocol for Suppressing Reproductive Functions in Rams Using Encapsulated Ovalbumin-Lhrh Protein
(Agricultural Research Communication Centre, 2018) Yilmaz, Ayhan; Karakus, Ferda; Hakan, Bunyamin; Stormo, Keith; Cengiz, Firat; Ulker, Hasan
The objective of study were to determine the effectiveness of Ovalbumin-LHRH-7 (OL) protein administered with cytosine guanine (CpG) adjuvant and Incomplete Freund's Adjuvant (IFA), and used one-shot immunization (single-dose vaccination) protocol in which booster dose included in microspheres in rams. Fifty ram lambs at about a year old were used. Treatment groups receiving Ovalbumin LHRH and control contained 10 animals. They were stratified according to age (weeks), live weight and scrotal circumference size, and were randomly assigned to five groups. Scrotal circumference, sexual activities and the numbers of rams having sperm in the ejaculate were affected from treatment (P<0.05) depending on the dose and vaccination protocol. However, immunization did not affect live weight changes in any treatment groups (P>0.05). Findings clearly demonstrate that the effects of OL immunization on reproductive traits in yearling rams were prominent when it was administered at higher dose and classical one primary and one booster immunization as free protein form. Also we observed that the effect of higher and single dose of OL protein in encapsulated form on reproductive traits had the partial suppressing. CpG adjuvant along with IFA was proved to be an effective adjuvant and could be suggested to be used and alternative to FCA in hormone immunization.
The Use of Encapsulated Ovalbumin-Lhrh (Ol) Protein in Single-Dose Vaccination Protocols for Lhrh Immunization
(Polska Akad Nauk, inst Genetyki I Hodowli Zwierzat, 2013) Ulker, Hasan; Stormo, Keith; deAvila, David; Reeves, J. Jerry
The objective of this study was to evaluate effectiveness of Ovalbumin-LHRH-7 (OL) protein when injected in crude, purified, free or encapsulated forms and using a single vaccination protocol along with CpG, inulin and saponin adjuvants. Fifty six C57BL/6 mice in seven groups (n=8) received various treatments and doses: Group 1 was control; Group 2 and 3 were injected twice with purified or crude OL protein, respectively, 4 wks apart. Group 4 and 5 were injected only once with purified or crude OL protein, respectively. Group 6 was injected only once with a mix of purified OL protein and encapsulated purified OL protein. Group 7 was injected only once with a mix of crude OL protein and encapsulated crude OL protein. There was an immunization effect observed on the I-125 LHRH % binding (P<0.05). Antibodies (Abs) against LHRH were identified on week 5 of immunization in groups 2, 3 and 4. Boosting at week 5 caused a significant increase in LHRH antibody (Ab) concentrations in groups 2 and 3. Numbers of pregnant animals and prengnancy rates were suppressed in all treatment groups at various degrees (P<0.05). Numbers of pups born were affected by immunization (P<0.05). Concluding, immunization with OL protein generated either biological or both immunological and biological effects in the most of treatment groups. The study confirmed the earlier findings that purified OL protein with CpG adjuvant is effective in inducing immune response and suppressing reproductive functions. However, the original idea that the non-capsulated antigen/adjuvant mix would work as primary injection, while encapsulated counterpart would mimic booster injections in a single vaccination protocol could not be confirmed in this study. Further studies to determine affecting factors for single-injection LHRH immunization are needed.