Browsing by Author "Sulaiman, Seerwan Hamadameen"

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The Analytical Evolution of Glibenclamide (Glyburide) Antidiabetic Agents: Analytical Tools, Challenges, and Future Trends
(Taylor & Francis inc, 2025) Barzani, Hemn A. H.; Anwar Omer, Rebaz; Salih Barzani, Khalamala Ibrahim; Sulaiman, Seerwan Hamadameen; Ali, Hoshyar Saadi
Glyburide (glibenclamide), a second-generation sulfonylurea, remains a cornerstone in the management of type 2 diabetes mellitus. However, its narrow therapeutic index and typically low plasma concentrations demand highly sensitive and reliable quantification methods for applications spanning pharmacokinetics, therapeutic drug monitoring, clinical diagnostics, pharmaceutical quality control, and environmental surveillance. This review provides a critical overview of four decades of methodological progress in glyburide analysis, synthesizing evidence from major scientific databases, including Scopus, Web of Science, ScienceDirect, PubMed, and Google Scholar. High-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) have emerged as the gold-standard approaches, offering exceptional sensitivity, specificity, and reproducibility across diverse matrices. In contrast, spectrophotometric methods provide cost-effective and straightforward options for routine analyses, while electrochemical techniques, particularly voltammetry with nanotextured electrodes, demonstrate remarkable sensitivity, sustainability, and applicability to trace detection. Capillary electrophoresis, though less commonly adopted, offers distinct benefits in resolution and solvent economy. Critical challenges are highlighted, including the quantification of glyburide at clinically relevant levels (2-400 ng/mL in plasma, with LOD as low as 0.038 ng/mL and LOQ 1.5 ng/mL), as well as overcoming matrix interferences and stability issues. Rigorous method validation remains essential to ensure accuracy and clinical utility. Finally, this review identifies key future directions, emphasizing the integration of green analytical chemistry principles, miniaturized platforms, and nanostructured sensing technologies to advance selective, sensitive, and environmentally sustainable glyburide analysis. These innovations hold the potential to transform glyburide monitoring from laboratory-based methods to real-world applications, including point-of-care testing and environmental health monitoring.
Analytical Techniques for Methyldopa and Metabolites: A Comprehensive Review
(Taylor & Francis Ltd, 2025) Barzani, Hemn A. H.; Sulaiman, Seerwan Hamadameen; Omer, Rebaz Anwar; Mer, Ali Hussein; Ali, Hoshyar Saadi
Methyldopa, a centrally acting alpha 2-adrenergic agonist, remains a key antihypertensive drug, particularly prescribed for pregnant and renal-impaired patients. Its clinical significance has led to extensive research aimed at developing reliable analytical methods for its accurate, sensitive, and selective determination in pharmaceutical formulations and biological matrices. Relevant literature was retrieved from Scopus, Web of Science, ScienceDirect, PubMed, and Google Scholar, restricted to English-language publications. This review critically examines the diverse analytical approaches used for Methyldopa quantification, outlining their principles, advantages, limitations, and applicability in both advanced and resource-limited settings. Chromatographic methods, especially high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), remain the most robust techniques, offering excellent sensitivity (LOD: 10-50 ng/mL for HPLC; as low as 0.7-15 ng/mL for LC-MS/MS) with rapid analysis times. While LC-MS/MS provides superior detection, it is limited by high costs and technical expertise requirements. Electrochemical methods, particularly voltammetry, stand out for their affordability, rapid analysis, and feasibility in decentralized laboratories, achieving LOD values as low as 0.01-0.05 mu M. Spectrophotometric approaches, primarily UV-Vis, remain the simplest and most cost-effective options, making them useful for routine quality control, though with reduced selectivity and higher detection limits. Key analytical challenges include Methyldopa's low concentration in biological fluids, chemical instability, and matrix interferences. This review provides a comparative evaluation of chromatographic, spectrophotometric, and electrochemical techniques, emphasizing the need for portable, low-cost platforms to expand accessibility in therapeutic monitoring. Overall, it offers critical insights for advancing Methyldopa analysis and improving clinical management in diverse healthcare settings.
Comprehensive Evaluation of Analytical Techniques for the Quantification of Etoposide in Various Matrices
(Taylor & Francis Inc, 2025) Barzani, Hemn A. H.; Sulaiman, Seerwan Hamadameen; Omer, Rebaz Anwar; Ahmad, Sarbast Naser; Ali, Hoshyar Saadi
Etoposide (ETO) is a semi-synthetic derivative of podophyllotoxin that is a common topoisomerase II inhibitor in the care of testicular cancer, small-cell lung cancer, leukemias, and lymphomas. Though the utility of ETO is extensive, it is constrained by variable bioavailability, a narrow therapeutic index, and potential for severe toxicities, which necessitate accurate quantification of ETO in pharmaceutical, clinical, and environmental applications. For more than four decades, measures of ETO have been described and developed. Spectrophotometric methods offer simplicity and low cost but lack specificity for complex matrices. High-performance liquid chromatography (HPLC) remains the reference standard, particularly when coupled with UV, fluorescence, or mass spectrometry (LC-MS or MS/MS), and also serves as the established method for pharmacokinetics and therapeutic drug monitoring. Other techniques, including capillary electrophoresis and emerging analytical methods, offer complementary advantages for resolution-challenged applications, thereby enhancing quality in ultra-trace and portable approaches. This review provides a narrative account of all aspects of these measures, specifically considering the basic operating principles, advantages, and disadvantages of each, the complexity of stability, matrix challenges, and ultra-trace measures. The future perspective is the need for greener, cost-effective, and clinically adapted clinical technologies, which will ultimately improve etoposide monitoring practices and patient outcomes.