Browsing by Author "Türkoǧlu, S."

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Assay of Mycobacterium Tuberculosis in Biopsy Specimens Taken From Hepatic Granuloma Patients Using Polymerase Chain Reaction Method
(2001) Tuncer, I.; Köksalan, O.K.; Demir, K.; Dinçer, D.; Türkoǧlu, S.; Kaymakoǧlu, S.; Çakaloǧlu, Y.
Background/aims: In the diagnosis of hepatic tuberculosis, classical laboratory methods (including cultures and direct staining of the acid resistant bacilli) are insufficient. Diagnosis is commonly made by clinical and histological findings and then confirmed by response to antituberculous treatment. In the diagnosis of pulmonary and extra-pulmonary tuberculosis, the use of PCR to detect tuberculous bacilli is being evaluated and some authors consider it a very reliable and sensitive method. However, the use of PCR in the diagnosis of hepatic tuberculosis has not been sufficiently supported by clinical studies. The aim of this study was to assess the sensitivity of this method in demonstrating M. tuberculosis bacilli in liver tissue specimens of hepatic granuloma patients, which were fixed with formalin and imbedded in paraffin blocks. Methods: Thirty-two liver biopsy specimens taken from cases diagnosed with granulomatous hepatitis and fixed with formalin in paraffin blocks were included in the study. The specimens were divided into three groups according to clinical and laboratory findings, histopathological diagnosis of hepatic granuloma and the response to appropriate treatment: Group A (n=12): hepatic granuloma with caseification necrosis (liver tuberculosis), Group B (n=10): noncaseous hepatic granuloma (liver tuberculosis), Group C (n=10) nontuberculous hepatic granuloma patients. All biopsy materials were stained by the Ziehl-Neelsen method and with allocrom stain in a direct search for bacilli and the PCR study was then performed. DNA amplification of M. tuberculosis IS6110 gene was also done by PCR study Results: Direct stains were negative in all specimens. M. tuberculosis gene amplification was found to be positive with the first step PCR method in three (25%) of 12 patients in group A, while with nested PCR, four (33%) of 12 patients in group A, three (30%) of 10 patients in group B and one (10%) of 10 patients in group C were positive. Only seven (32%) of all 22 patients of groups A and B who were diagnosed with tuberculous granulomatous hepatitis had positive PCR tests. In the control group however, only one (10%) of the patients had a positive PCR test. Conclusion: The findings of this study show that in our patient groups, PCR is insufficiently sensitive in diagnosing TB. Possible explanations for this low sensitivity could be that DNA concentrations were low in our specimens that paraffin blocks used contained inhibitors or that the primers used were inappropriate.
Is There a Relationship Between the Histopathological Features and Anatomical Locations of Thrombi Obtained During Endovascular Thrombectomy in Acute Ischemic Stroke and Its Comorbid Diseases
(Multidisciplinary Digital Publishing Institute (MDPI), 2026) Türkoǧlu, S.; Akdeniz, H.; Günsoy, E.; Yalınkılıç, F.A.
Objective: This study aimed to assess the association between the histopathological characteristics of thrombi extracted during endovascular thrombectomy and clinical factors, including the location of the occlusion, comorbid conditions, and treatment effectiveness, in patients with acute ischemic stroke. Materials and Methods: A total of 57 patients with acute ischemic stroke who underwent endovascular thrombectomy between 1 January 2022 and 31 December 2024 were included in the study. Thrombi were analyzed histopathologically and classified into categories based on their composition (RBC-dominant, fibrin-dominant, RBC = fibrin, organized fibrin) and phase (early or late stage). CD34 staining was used to assess organized fibrin. Results: The mean age of the patients was 65.2 ± 15.3 years, 52.6% were female, and 47.4% were male. The majority of thrombi were retrieved from the MCA M1 segment (64.9%). Histopathological analysis revealed that 49.1% of thrombi were RBC-dominant, 21.1% RBC = fibrin, 19.3% fibrin-dominant, and 10.5% contained organized fibrin. Early-stage thrombi accounted for 70.2% of cases, while late-stage thrombi comprised 29.8%. Thrombus composition was significantly associated with anatomical location, with RBC-dominant thrombi being most prevalent in the proximal ICA (88.2%; p < 0.001). CD34 staining identified organized fibrin in 10.5% of thrombi, exclusively in patients who underwent stent placement. However, no statistically significant correlation was identified between CD34 positivity and thrombus composition (p > 0.05). Additionally, no notable associations were found between thrombus composition and chronic comorbidities. Conclusions: Thrombus composition and stage exhibit variability depending on anatomical location, particularly in the proximal ICA, where RBC-dominant thrombi are more frequent. Although CD34 positivity indicates organized fibrin, it does not show a significant relationship with thrombus characteristics or patient comorbidities. These findings underscore the complex interplay between thrombus histopathology, anatomical location, and procedural outcomes, highlighting the need for further investigation. © 2025 by the authors.
Predictive Value of Non-Contrast Brain CT Patterns in Differentiating Aneurysmal and Non-Aneurysmal Subarachnoid Hemorrhage
(Elsevier Inc., 2026) Türkoǧlu, S.; Günsoy, E.
Introduction This study aims to evaluate the prognostic value of CT hemorrhage patterns in identifying aneurysmal versus non-aneurysmal subarachnoid hemorrhage (SAH) and in predicting aneurysm localization, to improve early diagnosis and treatment. Methods A retrospective study included 246 patients with spontaneous SAH who underwent CT and digital subtraction angiography (DSA) from 2020 to 2024. CT hemorrhage patterns were classified into eight types, and their correlation with aneurysm presence and location was analyzed. Statistical analysis was performed using SPSS, with odds ratios (OR) calculated to assess the association between CT patterns and aneurysm localization. Results Aneurysms were identified in 67.1% of patients, with the most common locations being the anterior communicating artery (ACom) and right middle cerebral artery (RMCA). A significant association was found between aneurysms and certain CT hemorrhage patterns. Type 1 (basal cistern) and Type 4 (ventricular extension) were most strongly linked to aneurysms, with Type 4B and Type 4R patterns predominantly seen in anterior-circulation aneurysms. In contrast, Type 8 patterns, associated with perimesencephalic hemorrhage, were more frequent in non-aneurysmal cases, often linked to vascular malformations such as arteriovenous malformations (AVMs) and fistulas. Conclusion This CT-based pattern classification serves as a complementary triage tool; these CT patterns may provide insight into vascular lesion location but do not replace DSA, the gold standard for definitive vascular imaging. Further research is needed to confirm these findings and assess their broader clinical applicability. © 2026 The Association of University Radiologists.