Browsing by Author "Takci, Z."

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Decreased Serum Paraoxonase and Arylesterase Activities in Patients With Rosacea
(Wiley, 2015) Takci, Z.; Bilgili, S. G.; Karadag, A. S.; Kucukoglu, M. E.; Selek, S.; Aslan, M.
Background: Recent evidence suggests that oxidative stress may be an important phenomenon in the pathophysiology of rosacea. Paraoxonase-1 (PON1) is an antioxidant enzyme with three activities: paraoxonase, arylesterase and dyazoxonase. In this study, we evaluated serum paraoxonase and arylesterase activities, and serum lipid hydroperoxide (LOOH) levels in patients with rosacea in comparison to healthy controls. Material and methodThe study included 39 rosacea patients and healthy controls, consisting of 40 age- and sex-matched healthy volunteers. Serum paraoxonase and arylesterase activities were measured using paraoxon and phenylacetate substrates. Serum LOOH levels were measured with the ferrous ion oxidation-xylenol orange assay. ResultsIn rosacea group mean serum paraoxonase and arylesterase activities were 74.5438.30UL(-1) and 141.29 +/- 22.27kUL(-1) respectively, which were significantly lower than controls (P=0.010, 0.005; respectively). Mean serum LOOH level of rosacea group was 8.17 +/- 1.91molL(-1) which was significantly higher than controls (P=0.009). There were no statistically significant differences between the clinical subtypes of the disease, menopause situation or ocular involvement with the respect to the serum paraoxonase and arylesterase activities and LOOH levels (all; P>0.05). ConclusionsSerum PON1 enzyme activities have decreased significantly in rosacea. These findings support that decreased PON1 activity and increased oxidative stress may play a role in the pathogenesis of rosacea. Further studies are needed to elucidate the role of PON1 activity in the pathophysiology of rosacea.
Effect of Systemic Isotretinoin Therapy on Mucociliary Clearance and Nasal Surface Mucosa in Acne Patients
(2013) Takci, Z.; Simsek, G.G.; Karabulut, H.; Buran, Y.; Karadag, A.S.
Background: Currently, there are no studies investigating the topical or systemic effects of retinoids on human nasal mucosa. We aimed to investigate the effect of systemic isotretinoin therapy on mucociliary transport and nasal surface mucosa using the saccharine test (ST) and nasal cytology techniques. Methods: A total of 30 patients with severe or moderate acne were enrolled in this study. The median prescribed dose of isotretinoin was 0.75 mg per kg per day. Clinical and biochemical examinations were carried out periodically. The ST and nasal cytology were performed before treatment and during the third month of therapy. Results: Of the 30 patients who initially agreed to participate in the research, 21 completed the study (18 female and 3 male, mean ± standard deviation (SD) aged 20.9 ± 4.7 years, range 15-32 years). There was a significant difference between the mucociliary clearance time for subjects in the pre- and post-treatment periods (173.8 ± 89.2 seconds vs 245.2 ± 191.6 seconds, respectively; P=.009). Cytological examination revealed that the squamous cell ratio was significantly lower and the reactive changes of the respiratory epithelium were significantly higher 3 months after isotretinoin therapy than before therapy (P=.010, P=.002, respectively). There were mild signs of inflammation according to the number of neutrophilic leukocytes (8.3% vs 26.6%, P=.06) after 3 months of isotretinoin therapy. Conclusion: Systemic isotretinoin alters the mucociliary transport, decreases the squamous cell ratio, increases the reactive changes in the respiratory epithelium significantly, and increases neutrophils in the nasal surface mucosa in the third month of treatment. Copyright © 2013 Journal of Drugs in Dermatology.
Elevated Insulin Resistance in Patients With Recurrent Aphthous Stomatitis
(Springer Heidelberg, 2015) Takci, Z.; Karadag, A. S.; Ertugrul, D. T.; Bilgili, S. G.
Objectives The role of glucose metabolism disorders in periodontal diseases including recurrent aphthous stomatitis (RAS) is currently attracting attention. The aim of this study is to investigate insulin resistance (IR) in patients with RAS in otherwise healthy individuals. Materials and methods The study enrolled 81 patients with RAS and 61 healthy control subjects. Blood glucose, insulin, C-peptide, hemoglobin A1c, total cholesterol (TC), high-density lipoprotein (HDL) cholesterol, and HOMA-IR were measured in each individual. Results Forty-two of the RAS group were in the active, and 39 of the RAS group were in the passive stage. The levels of c-peptide, insulin, and HOMA-IR were remarkably higher in the RAS group (p=0.015; p<0.0001; p<0.0001, respectively) than the control group. The levels of HbA1c, glucose (p=0,045), TC (p=0,008), HDL cholesterol (p=0,002), and HOMA-IR (p=0.022) were significantly higher in patients with RAS in the active stage. Conclusion The study revealed an elevated IR in patients with RAS that was not previously shown. IR was more prominent when the patients were in the active stage that elevated inflammatory cytokines may induce IR by interfering with insulin signaling. Further studies, investigating the interplay between RAS, inflammation, and IR are needed. Clinical relevance Patients who admitted the hospital with RAS might be screened for prediabetes.
Immunoregulatory Effects of Isotretinoin in Patients With Acne
(Wiley-blackwell, 2012) Karadag, A. S.; Ertugrul, D. T.; Bilgili, S. G.; Takci, Z.; Akin, K. O.; Calka, O.
Background In vitro studies have shown that retinoids influence T-cell differentiation. Objectives To study the effect of isotretinoin on T-cell differentiation markers in patients with acne. Methods A total of 37 patients with acne vulgaris (25 female, 12 male, age 19.6 +/- 3.7 years) and 30 age- and sex-matched healthy controls (19 female, 11 male, age 20.5 +/- 4.4 years) were included in the study. Screening for biochemical parameters in serum samples were done just before initiation (pretreatment) and after 3 months of isotretinoin treatment (post-treatment) in the acne group. Results Baseline levels of tumour necrosis factor (TNF)-alpha (P < 0.0001), interleukin (IL)-4 (P < 0.0001), IL-17 (P < 0.0001) and interferon (IFN)-gamma (P = 0.002) were significantly higher in the acne group compared with the control group. TNF-alpha (P < 0.0001), IL-4 (P < 0.0001), IL-17 (P < 0.0001) and IFN-gamma (P < 0.0001) levels decreased after isotretinoin treatment. TNF-alpha and IL-4 values after isotretinoin treatment were similar to those of the control group. However, levels of IL-17 (P < 0.0001) after isotretinoin treatment were higher than those of the control group, despite a significant decline after treatment. Levels of IFN-gamma (P < 0.0001) after isotretinoin treatment were lower than those of the control group. Conclusions This study shows that isotretinoin treatment significantly decreases TNF, IL-4, IL-17 and IFN-gamma levels in patients with acne. We failed to show that isotretinoin redirects naive T helper (Th) differentiation preferentially towards the Th2 cell lineage.
Response To 'letter To the Editor' by Agilli Et Al. Entitled 'assessment of Decreased Serum Paraoxonase Activity in Patients With Rosacea in Terms of Methodology
(Wiley-blackwell, 2016) Takci, Z.; Bilgili, S. G.; Karadag, A. S.; Kucukoglu, M. E.; Selek, S.; Aslan, M.