Browsing by Author "Tekbudak, Ilhan Kaya"

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Comparative Karyological Analysis of Some Turkish Cuscuta L. (Convolvulaceae)
(Firenze Univ Press, 2022) Tasar, Neslihan; Tekbudak, Ilhan Kaya; Demir, Ibrahim; Acar, Mikail; Kursat, Murat
This study investigated the somatic chromosome numbers and morphomet-ric properties of 11 different taxa belonging to the genus Cuscuta L., which is one of the parasitic flowering plants and causes significant economic losses on agricultural products. For this purpose, the species were examined karyologically and compared statistically. Belonging to the genus Cuscuta, C. campestris Yunck., C. hyalina Roth, C. kotschyana Boiss., C. babylonica Aucher ex Choisy, C. europaea L., C. kurdica Engelm., C. brevistyla A.Braun ex A.Rich, C. planiflora Ten., C. approximata Bab., C. lupuliformis Krock. C. palaestina Boiss. the chromosome number and morphology of the species were investigated using karyological techniques. Chromosome numbers of the species; C. kotschyana, C. babylonica, C. europaea, C. kurdica, C.planiflora 2n=14; C. campes-tris, C. hyalina, C. approximata, C. lupuliformis, and C. palaestina 2n=28 and C. brevi-styla 2n=42 is determined. Also, the species' chromosome number, total chromosome length, relative length, arm ratio, centromere index and centromere states, and karyo-type asymmetry values were determined. Chromosome numbers of C. kotschyana and C. kurdica taxa were defined for the first time in this study. Thus, new data on the sys-tematics of these species have been revealed.
Determining Phylogenetic Relationships of Some Endemic and Rare Astragalus Taxa in the Van Lake Basin
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2023) Genli, Gulistan; Tekbudak, Ilhan Kaya; Usta, Mustafa; Mukemre, Muzaffer
Astragalus, the most diverse genus in the world, contains about 3000 different taxa. This genus has 489 taxa and 63 sections in Turkey, giving it a 51% endemism rate. 82 Astragalus taxa were collected from the Van Lake Basin in 2019 for this research. These species' morphological diagnoses revealed that four of them were rare and 17 of them were endemic. In this research, the universal primers ITS4 and ITS5 were used to amplify the ITS1 and ITS2 sections, which included the 5.8S gene of rDNA. Different programs (SnapGene, CLC DNA Workbench) were used to analyze the genome information of species in the genus Astragalus. The DNA sequences of Astragalus species have been uploaded to the GenBank database of the National Center for Biotechnology Information (NCBI) (Maryland, USA), which is open to all researchers worldwide. The length of the ITS in the study, including the 5.8S sequence, varied from 669 to 687 bp. The results showed that the phylogenetic tree combined with the most stable secondary (20) structure obtained from the universal ITS4 and ITS5 primers is an effective tool for the identification of Astragalus taxa.
Seed Morphology of Cuscuta L. (Convolvulaceae) in Turkey and Its Systematic Importance
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Demir, Ibrahim; Angishan, Firat; Tekbudak, Ilhan Kaya
Seed morphologies of 14 Cuscuta taxa from Turkey were examined by patterns of macro and micro morphological features were determined. Seed epidermis cell shapes were generally either irregular or polygonal. The surface pattern was mostly reticulate. This research revealed that Cuscuta species in Turkey have different seed morphologies. The results obtained will aid in the taxonomic evaluation of morphologically closely related species.